汾河入黄口夏季微生物群落结构分析
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摘要
【背景】河流交汇区日益成为流域生态治理的焦点和热点之一。【目的】探明汾河入黄口微生物群落结构及其主要环境影响因子。【方法】应用16S rRNA基因Illumina MiSeq高通量测序技术,分析了汾河入黄口夏季微生物群落结构,并利用典范对应分析(Canonical correspondence analysis,CCA)了解影响微生物群落的主要环境因子。【结果】多样性指数分析表明该区域微生物群落多样性较高。微生物多样性分析发现优势菌门为变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)和放线菌门(Actinobacteria);在属分类水平上,相对丰度最高的菌属为芽孢杆菌属(Bacillus),其次为乳球菌属(Lactococcus)和hgcI_clade。Spearman相关性分析及典范对应分析表明环境因子对水体微生物群落结构具有显著影响。【结论】汾河与黄河微生物群落组成具有一定的差异,不同环境因子对不同微生物的影响程度不同,p H和溶解氧(Dissolved oxygen,DO)是汾河入黄口微生物群落结构的主要影响因子。
[Background] River confluences have become a focus in ecological management of a river basin. [Objective] The microbial community structure and main influencing environmental factors at the estuary of the Fenhe River into the Yellow River were studied. [Methods] The microbial community structure at estuary of the Fenhe River into the Yellow River in summer was analyzed by means of 16 S rRNA gene-based Illumina MiSeq high-throughput sequencing, and the main environmental factors affecting the microbial community were identified by canonical correspondence analysis. [Results] The alpha-diversity analysis revealed that the diversity of microbial community was high in this area. Microbial diversity analysis indicated that the dominant bacterial phyla were Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria; at genus level of taxonomic criteria, the dominant genus was Bacillus, followed by Lactococcus and hgcI_clade. Spearman correlation analysis and canonical correspondence analysis(CCA) showed that environmental factors had significant effects on the microbial community structure of water. [Conclusion] The microbial community composition was different between the Fenhe River and the Yellow River, different environmental factors had distinct effects on diverse microorganisms. The microbial communities were mostly affected by pH and dissolved oxygen(DO) at the estuary of the Fenhe River into the Yellow River.
[Background] River confluences have become a focus in ecological management of a river basin. [Objective] The microbial community structure and main influencing environmental factors at the estuary of the Fenhe River into the Yellow River were studied. [Methods] The microbial community structure at estuary of the Fenhe River into the Yellow River in summer was analyzed by means of 16 S rRNA gene-based Illumina MiSeq high-throughput sequencing, and the main environmental factors affecting the microbial community were identified by canonical correspondence analysis. [Results] The alpha-diversity analysis revealed that the diversity of microbial community was high in this area. Microbial diversity analysis indicated that the dominant bacterial phyla were Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria; at genus level of taxonomic criteria, the dominant genus was Bacillus, followed by Lactococcus and hgcI_clade. Spearman correlation analysis and canonical correspondence analysis(CCA) showed that environmental factors had significant effects on the microbial community structure of water. [Conclusion] The microbial community composition was different between the Fenhe River and the Yellow River, different environmental factors had distinct effects on diverse microorganisms. The microbial communities were mostly affected by pH and dissolved oxygen(DO) at the estuary of the Fenhe River into the Yellow River.
引文
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[17]Tang J,Xu XR,Shang CY,et al.Association of bacterial diversity in city area of Nanming river with environmental factors[J].Acta Microbiologica Sinica,2015,55(8):1050-1059(in Chinese)唐婧,徐晓蓉,商传禹,等.南明河城区河段细菌多样性与环境因子的关系[J].微生物学报,2015,55(8):1050-1059
[18]Quast C,Pruesse E,Yilmaz P,et al.The SILVA ribosomal RNAgene database project:improved data processing and web-based tools[J].Nucleic Acids Research,2013,41(D1):590-596
[19]Bates ST,Clemente JC,Flores GE,et al.Global biogeography of highly diverse protistan communities in soil[J].The ISMEJournal,2013,7(3):652-659
[20]Claesson MJ,Wang Q,O’Sullivan O,et al.Comparison of two next-generation sequencing technologies for resolving highly complex microbiota composition using tandem variable 16SrRNA gene regions[J].Nucleic Acids Research,2010,38(22):e200
[21]Liu C,Li JB,Rui JP,et al.The applications of the 16S rRNA gene in microbial ecology:current situation and problems[J].Acta Ecologica Sinica,2015,35(9):2769-2788(in Chinese)刘驰,李家宝,芮俊鹏,等.16S rRNA基因在微生物生态学中的应用[J].生态学报,2015,35(9):2769-2788
[22]Xia N,Xia XH,Liu T,et al.Characteristics of bacterial community in the water and surface sediment of the Yellow River,China,the largest turbid river in the world[J].Journal of Soils and Sediments,2014,14(11):1894-1904
[23]Jackson CR,Millar JJ,Payne JT,et al.Free-Living and particle-associated bacterioplankton in large rivers of the Mississippi River Basin demonstrate biogeographic patterns[J].Applied and Environmental Microbiology,2014,80(23):7186-7195
[24]Newton RJ,Jones SE,Eiler A,et al.A guide to the natural history of freshwater lake bacteria[J].Microbiology and Molecular Biology Reviews,2011,75(1):14-49
[25]Ren LJ,He D,Xing P,et al.Bacterial diversity and ecological function in lake water bodies[J].Biodiversity Science,2013,21(4):421-432(in Chinese)仸丼娟,何聃,邢鹏,等.湖泊水体细菌多样性及其生态功能研究迚展[J].生物多样性,2013,21(4):421-432
[26]Liu ZH,Huang SB,Sun GP,et al.Phylogenetic diversity,composition and distribution of bacterioplankton community in the Dongjiang River,China[J].FEMS Microbiology Ecology,2012,80(1):30-44
[27]Jones SE,Newton RJ,McMahon KD.Evidence for structuring of bacterial community composition by organic carbon source in temperate lakes[J].Environmental Microbiology,2009,11(9):2463-2472
[28]Jeon EM,Kim HJ,Jung K,et al.Impact of Asian dust events on airborne bacterial community assessed by molecular analyses[J].Atmospheric Environment,2011,45(25):4313-4321
[29]Wang LH,LüZ,Hao CB,et al.Degrading bacteria community structure in groundwater of a petroleum-contaminated site[J].Environmental Science and Technology,2013,36(7):1-8,146(in Chinese)王丼华,吕铮,郝春博,等.某石油污染场地地下水中降解菌群落结构研究[J].环境科学与技术,2013,36(7):1-8,146
[2]Yan WJ,Mayorga E,Li XY,et al.Increasing anthropogenic nitrogen inputs and riverine DIN exports from the Changjiang River basin under changing human pressures[J].Global Biogeochemical Cycles,2010,24(4):GB0A06
[3]Hao LX,Sun RH,Chen LD.Health assessment of river ecosystem in Haihe River basin,China[J].Environmental Science,2014,35(10):3692-3701(in Chinese)郝利霞,孙然好,陈利顶.海河流域河流生态系统健康评价[J].环境科学,2014,35(10):3692-3701
[4]Dong P,Sun YJ,Wang HQ,et al.Study of micro-ecosystem of the Wenyu River by terminal restriction fragment length polymorphism analysis[J].China Environmental Science,2011,31(4):631-636(in Chinese)董萍,孙寓姣,王红旗,等.利用T-RFLP技术对温榆河微生物群落结构研究[J].中国环境科学,2011,31(4):631-636
[5]Cébron A,Garnier J.Nitrobacter and Nitrospira genera as representatives of nitrite-oxidizing bacteria:detection,quantification and growth along the lower Seine River(France)[J].Water Research,2005,39(20):4979-4992
[6]Findlay S.Stream microbial ecology[J].Journal of the North American Benthological Society,2010,29(1):170-181
[7]Jiang JG,Shen YF.Development of the microbial communities in Lake Donghu in relation to water quality[J].Environmental Monitoring and Assessment,2007,127(1/3):227-236
[8]Li ZH.Spatial and seasonal diversity of bacterioplankton communities in Lake Dongping[D].Tai’an:Master’s Thesis of Shandong Agricultural University,2010(in Chinese)李正华.东平湖浮游细菌群落多样性分析[D].泰安:山东农业大学硕士学位论文,2010
[9]Wang P,Chen B,Li CQ,et al.Bacterial communities in Nanchang section of the Ganjiang River in wet seaon[J].China Environmental Science,2016,36(8):2453-2462(in Chinese)王鹏,陈波,李传琼,等.赣江南昌段丰水期细菌群落特征[J].中国环境科学,2016,36(8):2453-2462
[10]Wei JM,Cui LJ,Li W,et al.Characteristics of bacterial communities in surface-flow constructed wetlands[J].Environmental Science,2016,37(11):4357-4365(in Chinese)魏佳明,崔丼娟,李伟,等.表流湿地细菌群落结构特征[J].环境科学,2016,37(11):4357-4365
[11]Zhang YJ,Li K,Zhu HR,et al.Community structure of microorganisms and its seasonal variation in Beihai Lake[J].Environmental Science,2017,38(8):3319-3329(in Chinese)张雅洁,李珂,朱浩然,等.北海湖微生物群落结构随季节变化特征[J].环境科学,2017,38(8):3319-3329
[12]Chen ZJ,Ding CY,Zhu JY,et al.Community structure and influencing factors of bacterioplankton during low water periods in Danjiangkou Reservoir[J].China Environmental Science,2017,37(1):336-344(in Chinese)陈兆迚,丁传雨,朱静亚,等.丹江口水库枯水期浮游细菌群落组成及影响因素研究[J].中国环境科学,2017,37(1):336-344
[13]Staley C,Johnson D,Gould TJ,et al.Frequencies of heavy metal resistance are associated with land cover type in the Upper Mississippi River[J].Science of the Total Environment,2015,511:461-468
[14]Liu R,Wu W,Zhou XD,et al.Bacterioplankton community structure in Weihe River and its relationship with environmental factors[J].Acta Scientiae Circumstantiae,2017,37(3):934-944(in Chinese)刘睿,吴巍,周孝德,等.渭河浮游细菌群落结构特征及其关键驱动因子[J].环境科学学报,2017,37(3):934-944
[15]Bai J,Liu XS,Hou R,et al.Community structure and influencing factors of bacterioplankton in the southern South China Sea[J].China Environmental Science,2014,34(11):2950-2957(in Chinese)白洁,刘小沙,侯瑞,等.南海南部海域浮游细菌群落特征及影响因素研究[J].中国环境科学,2014,34(11):2950-2957
[16]Zwart G,Crump BC,Kamst-Van Agterveld MP,et al.Typical freshwater bacteria:an analysis of available 16S rRNA gene sequences from plankton of lakes and rivers[J].Aquatic Microbial Ecology,2002,28(2):141-155
[17]Tang J,Xu XR,Shang CY,et al.Association of bacterial diversity in city area of Nanming river with environmental factors[J].Acta Microbiologica Sinica,2015,55(8):1050-1059(in Chinese)唐婧,徐晓蓉,商传禹,等.南明河城区河段细菌多样性与环境因子的关系[J].微生物学报,2015,55(8):1050-1059
[18]Quast C,Pruesse E,Yilmaz P,et al.The SILVA ribosomal RNAgene database project:improved data processing and web-based tools[J].Nucleic Acids Research,2013,41(D1):590-596
[19]Bates ST,Clemente JC,Flores GE,et al.Global biogeography of highly diverse protistan communities in soil[J].The ISMEJournal,2013,7(3):652-659
[20]Claesson MJ,Wang Q,O’Sullivan O,et al.Comparison of two next-generation sequencing technologies for resolving highly complex microbiota composition using tandem variable 16SrRNA gene regions[J].Nucleic Acids Research,2010,38(22):e200
[21]Liu C,Li JB,Rui JP,et al.The applications of the 16S rRNA gene in microbial ecology:current situation and problems[J].Acta Ecologica Sinica,2015,35(9):2769-2788(in Chinese)刘驰,李家宝,芮俊鹏,等.16S rRNA基因在微生物生态学中的应用[J].生态学报,2015,35(9):2769-2788
[22]Xia N,Xia XH,Liu T,et al.Characteristics of bacterial community in the water and surface sediment of the Yellow River,China,the largest turbid river in the world[J].Journal of Soils and Sediments,2014,14(11):1894-1904
[23]Jackson CR,Millar JJ,Payne JT,et al.Free-Living and particle-associated bacterioplankton in large rivers of the Mississippi River Basin demonstrate biogeographic patterns[J].Applied and Environmental Microbiology,2014,80(23):7186-7195
[24]Newton RJ,Jones SE,Eiler A,et al.A guide to the natural history of freshwater lake bacteria[J].Microbiology and Molecular Biology Reviews,2011,75(1):14-49
[25]Ren LJ,He D,Xing P,et al.Bacterial diversity and ecological function in lake water bodies[J].Biodiversity Science,2013,21(4):421-432(in Chinese)仸丼娟,何聃,邢鹏,等.湖泊水体细菌多样性及其生态功能研究迚展[J].生物多样性,2013,21(4):421-432
[26]Liu ZH,Huang SB,Sun GP,et al.Phylogenetic diversity,composition and distribution of bacterioplankton community in the Dongjiang River,China[J].FEMS Microbiology Ecology,2012,80(1):30-44
[27]Jones SE,Newton RJ,McMahon KD.Evidence for structuring of bacterial community composition by organic carbon source in temperate lakes[J].Environmental Microbiology,2009,11(9):2463-2472
[28]Jeon EM,Kim HJ,Jung K,et al.Impact of Asian dust events on airborne bacterial community assessed by molecular analyses[J].Atmospheric Environment,2011,45(25):4313-4321
[29]Wang LH,LüZ,Hao CB,et al.Degrading bacteria community structure in groundwater of a petroleum-contaminated site[J].Environmental Science and Technology,2013,36(7):1-8,146(in Chinese)王丼华,吕铮,郝春博,等.某石油污染场地地下水中降解菌群落结构研究[J].环境科学与技术,2013,36(7):1-8,146
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