不同耐湿基因型芝麻响应湿害胁迫的生理指标变化的研究
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摘要
为探究湿害胁迫对不同湿害敏感性芝麻生理指标的影响,本研究以耐湿基因型湖北竹山白和湿害敏感基因型鄂芝2号为试验材料,于盛花期进行湿害处理,以湿害处理前材料为对照(CK),分别测定芝麻叶片渗透调节物质[游离脯氨酸(Pro)、可溶性蛋白(SP)及可溶性糖(SS)]相对含量,保护酶[抗坏血酸过氧化物酶(APX)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)]相对活性和丙二醛(MDA)相对含量。结果表明,湖北竹山白与鄂芝2号的SS相对含量和SOD相对活性的变化情况相似,二者均分别呈"W型"和"M型"变化趋势。湖北竹山白的SP相对含量和APX、CAT、POD相对活性始终明显高于或稍高于鄂芝2号,但鄂芝2号的Pro和MDA相对含量始终明显高于湖北竹山白,上述指标的变化趋势无明显规律。综上,对湿害胁迫的耐受程度湖北竹山白强于鄂芝2号。本研究为芝麻耐湿生理响应机理的深入研究及耐湿相关基因的挖掘提供了理论依据。
In order to test effects of waterlogging stress on physiological indexes of two different genotypes of sesame in this research, the content of osmotic adjustment substances(Pro, SP and SS), the activity of protective enzymes(APX, SOD, CAT and POD) and the content of MDA were investigated during waterlogging stress at different time points at the full flowering stage. The results showed that the variation tendency of the relative content of SS and the relative activity of SOD were similar in both genotypes, respectively presented "W-type" and "M-type" change trend. The relative content of SP and the relative activity of APX, CAT, POD in waterlogging-tolerant genotype Hubei Zhushanbaizhima were always obviously or slightly higher than sensitive genotype Ezhi2. However, the relative content of Pro and MDA in sensitive genotype Ezhi2 was significantly higher than waterlogging-tolerant genotype Hubei Zhushanbaizhima. There was no obvious regularity in these indicators. In conclusion, the tolerance of Hubei Zhushanbaizhima in waterlogging was stronger than Ezhi2. This research provided a theoretical basis for further clarifying the mechanism of physiological response of waterlogging resistance and identifying the waterlogging resistance genes in sesame.
In order to test effects of waterlogging stress on physiological indexes of two different genotypes of sesame in this research, the content of osmotic adjustment substances(Pro, SP and SS), the activity of protective enzymes(APX, SOD, CAT and POD) and the content of MDA were investigated during waterlogging stress at different time points at the full flowering stage. The results showed that the variation tendency of the relative content of SS and the relative activity of SOD were similar in both genotypes, respectively presented "W-type" and "M-type" change trend. The relative content of SP and the relative activity of APX, CAT, POD in waterlogging-tolerant genotype Hubei Zhushanbaizhima were always obviously or slightly higher than sensitive genotype Ezhi2. However, the relative content of Pro and MDA in sensitive genotype Ezhi2 was significantly higher than waterlogging-tolerant genotype Hubei Zhushanbaizhima. There was no obvious regularity in these indicators. In conclusion, the tolerance of Hubei Zhushanbaizhima in waterlogging was stronger than Ezhi2. This research provided a theoretical basis for further clarifying the mechanism of physiological response of waterlogging resistance and identifying the waterlogging resistance genes in sesame.
引文
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[3] 丁霞,王林海,张艳欣,黎冬华,危文亮,张秀荣. 我国芝麻主栽品种耐湿性鉴定分析[J]. 华北农学报,2012,27(4):89-93
[4] Li Z, Mei S, Mei Z, Liu X, Fu T, Zhou G, Tu J. Mapping of QTL associated with waterlogging tolerance and drought resistance during the seedling stage in oilseed rape (Brassica napus)[J]. Euphytica, 2014, 197(3):341-353
[5] Boru G, Ginkel M Ⅴ, Kronstad W E. Expression and inheritance of tolerance to waterlogging stress in wheat[J]. Euphytica, 2001, 117(2):91-98
[6] 赵竑绯,赵阳,张驰,徐小牛. 模拟淹水对杞柳生长和光合特性的影响[J]. 生态学报,2013,33(3):898-906
[7] 阚靖博,李丽娜,曲东,王保莉.淹水培养过程中水稻土细菌丰度与群落结构变化[J]. 生物多样性,2014,22(4):508-515
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[9] 王军,周美学,许如根,吕超,黄祖六. 大麦耐湿性鉴定指标和评价方法研究[J]. 中国农业科学,2007,40(10):2145-2152
[10] Leul M, Zhou W J. Alleviation of waterlogging damage in winter rape by uniconazole application: effects on enzyme activity, lipid peroxidation, and membrane integrity[J]. Journal of Plant Growth Regulation, 1999, 18(1):9-14
[11] 刘凯文,苏荣瑞,朱建强,吴启侠,田皓. 棉花苗期叶片关键生理指标对涝渍胁迫的响应[J]. 中国农业气象,2012,33(3):442-447
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[13] 王文泉,郑永战,梅鸿献,张福锁. 不同耐渍基因型芝麻在厌氧胁迫下根系的生理与结构变化[J]. 植物遗传资源学报,2003,4(3):214-219
[14] 王美荣. 淹水胁迫下芝麻苗期生理特性和蛋白质差异表达研究[D].郑州:郑州大学,2011
[15] Molaei P, Ebadi A, Namvar A. Water relation, solute accumulation and cell membrane injury in sesame (Sesamum indicum L.) cultivars subjected to water stress[J]. Annals of Biological Research, 2012, 3(4):1833-1838
[16] Heidari M, Galavi M, Hassani M. Effect of sulfur and iron fertilizers on yield, yield components and nutrient uptake in sesame (Sesamum indicum L.) under water stress[J]. African Journal of Biotechnology, 2011, 10(44):8816-8822
[17] 张根峰,张翼. 盛花期不同渍涝时间对芝麻生理指标及产量性状的影响[J]. 中国农学通报,2010,26(23):196-199
[18] 王林海,张艳欣,黎冬华,张晓燕,吕海霞,危文亮,孙建,张秀荣. 湿害胁迫对发芽期芝麻的影响及耐湿性评价方法建立[J]. 中国油料作物学报,2011,33(6):588-592
[19] 张艳欣,王林海,黎冬华,高媛,吕海霞,张秀荣. 芝麻耐湿性QTL定位及优异耐湿基因资源挖掘[J]. 中国农业科学,2014,47(3):422-430
[20] 孙建,张秀荣,张艳欣,黄波,车卓. 芝麻不同品种(系)耐湿性的综合评价[J]. 中国油料作物学报,2008,30(4):518-521
[21] 孙建,张秀荣,张艳欣,王林海,黄波. 湿害处理对不同生育时期芝麻叶片保护酶活性和种子产量的影响[J]. 应用与环境生物学报,2009,15(6):790-795
[22] 江诗洋,黎冬华,张艳欣,魏鑫,张秀荣,王林海. 水涝胁迫下芝麻内源激素及表型响应[J]. 中国油料作物学报,2015,37(5):676-682
[23] 范月仙,王鹏,李生泉,李锐,李娜,郝辉芳. 临界生长温度锻炼对棉苗抗冷性的影响[J]. 棉花学报,2011,23(1):90-93
[24] 毕颖,刘燕,张平.蒽酮比色法测定大豆乳清废水中可溶性总糖含量[J].大豆通报,2006(3): 24-25
[25] 高京草,王慧霞,李西选. 可溶性蛋白、丙二醛含量与枣树枝条抗寒性的关系研究[J]. 北方园艺,2010(23):18-20
[26] 李忠光,李江鸿,杜朝昆,黄号栋,龚明. 在单一提取系统中同时测定五种植物抗氧化酶[J]. 云南师范大学学报(自然科学版),2002,22(6):44-48
[27] 张蕊,吕俊,米青山,王三根. 低温下外源水杨酸对水稻幼苗抗氧化酶系的影响[J]. 西南农业大学学报(自然科学版),2006,28(1): 29-32
[28] 焦蓉,刘好宝,刘贯山,王树林,侯娜,王全贞,刘朝科,冯祥国,胡晓明,靳义荣. 论脯氨酸累积与植物抗渗透胁迫[J]. 中国农学通报,2011,27(7):216-221
[29] 汪妮. 渍涝胁迫对不同基因型芝麻生理特性及产量性状的影响[D].郑州:河南农业大学,2009
[30] Yoshiba Y, Kiyosue T. Regulation of levels of proline as osmolyte in plants under water stress [J].Plant and Cell Physiology, 1997, 38(10):1095-1102
[31] 段素梅,杨安中,黄义德,吴文革,许有尊,陈刚. 干旱胁迫对水稻生长、生理特性和产量的影响[J]. 核农学报,2014,28(6):1124-1132
[32] Garcia A B, Almeida-Enhler J, Iyer S. Effects of osmoprotectants upon NaCl stress in rice [J].Plant Physiology, 1997, 115(1):159-169
[33] Liu J, Zhu J K. Proline accumulation and salt-stress-induced gene expression in a salt-hypersensitive mutant of Arabidopsis [J]. Plant Physiology, 1997, 114(2):591
[34] 刘娥娥,宗会,郭振飞, 黎用朝. 干旱、盐和低温胁迫对水稻幼苗脯氨酸含量的影响[J]. 热带亚热带植物学报,2000,8(3):235-238
[35] 袁梦雅.干旱胁迫对植物保护酶的影响[J]. 科技信息, 2011 (14):105
[36] 韩轩,蔡萍,伊卫东,韩晓亮,徐振朋,宛诣超. 内蒙古8份冷蒿种质资源材料抗寒性的初步研究[J]. 草地学报,2014,22(3):669-672
[37] 吴敏,张文辉,周建云,马闯,韩文娟. 干旱胁迫对栓皮栎幼苗细根的生长与生理生化指标的影响[J]. 生态学报,2014,34(15):4223-4233
[38] 苗雨晨, 白玲, 苗琛, 陈珈, 宋纯鹏. 植物谷胱甘肽过氧化物酶研究进展[J]. 植物学通报, 2005, 22(3): 350-356
[39] Asada K. Ascorbate peroxidase: a hydrogen peroxide scavenging enzyme in plants [J]. Physiologia Plantarum, 1992, 85(2):235-241
[2] 钟雪花,杨万年,吕应堂. 淹水胁迫下对烟草、油菜某些生理指标的比较研究[J]. 武汉植物学研究,2002,20(5):395-398
[3] 丁霞,王林海,张艳欣,黎冬华,危文亮,张秀荣. 我国芝麻主栽品种耐湿性鉴定分析[J]. 华北农学报,2012,27(4):89-93
[4] Li Z, Mei S, Mei Z, Liu X, Fu T, Zhou G, Tu J. Mapping of QTL associated with waterlogging tolerance and drought resistance during the seedling stage in oilseed rape (Brassica napus)[J]. Euphytica, 2014, 197(3):341-353
[5] Boru G, Ginkel M Ⅴ, Kronstad W E. Expression and inheritance of tolerance to waterlogging stress in wheat[J]. Euphytica, 2001, 117(2):91-98
[6] 赵竑绯,赵阳,张驰,徐小牛. 模拟淹水对杞柳生长和光合特性的影响[J]. 生态学报,2013,33(3):898-906
[7] 阚靖博,李丽娜,曲东,王保莉.淹水培养过程中水稻土细菌丰度与群落结构变化[J]. 生物多样性,2014,22(4):508-515
[8] 张永吉. 不结球白菜耐湿性鉴定及其生理机制研究[D].南京:南京农业大学,2011
[9] 王军,周美学,许如根,吕超,黄祖六. 大麦耐湿性鉴定指标和评价方法研究[J]. 中国农业科学,2007,40(10):2145-2152
[10] Leul M, Zhou W J. Alleviation of waterlogging damage in winter rape by uniconazole application: effects on enzyme activity, lipid peroxidation, and membrane integrity[J]. Journal of Plant Growth Regulation, 1999, 18(1):9-14
[11] 刘凯文,苏荣瑞,朱建强,吴启侠,田皓. 棉花苗期叶片关键生理指标对涝渍胁迫的响应[J]. 中国农业气象,2012,33(3):442-447
[12] 杨鹏,胥晓. 淹水胁迫对青杨雌雄幼苗生理特性和生长的影响[J]. 植物生态学报,2012,36(1):81-87
[13] 王文泉,郑永战,梅鸿献,张福锁. 不同耐渍基因型芝麻在厌氧胁迫下根系的生理与结构变化[J]. 植物遗传资源学报,2003,4(3):214-219
[14] 王美荣. 淹水胁迫下芝麻苗期生理特性和蛋白质差异表达研究[D].郑州:郑州大学,2011
[15] Molaei P, Ebadi A, Namvar A. Water relation, solute accumulation and cell membrane injury in sesame (Sesamum indicum L.) cultivars subjected to water stress[J]. Annals of Biological Research, 2012, 3(4):1833-1838
[16] Heidari M, Galavi M, Hassani M. Effect of sulfur and iron fertilizers on yield, yield components and nutrient uptake in sesame (Sesamum indicum L.) under water stress[J]. African Journal of Biotechnology, 2011, 10(44):8816-8822
[17] 张根峰,张翼. 盛花期不同渍涝时间对芝麻生理指标及产量性状的影响[J]. 中国农学通报,2010,26(23):196-199
[18] 王林海,张艳欣,黎冬华,张晓燕,吕海霞,危文亮,孙建,张秀荣. 湿害胁迫对发芽期芝麻的影响及耐湿性评价方法建立[J]. 中国油料作物学报,2011,33(6):588-592
[19] 张艳欣,王林海,黎冬华,高媛,吕海霞,张秀荣. 芝麻耐湿性QTL定位及优异耐湿基因资源挖掘[J]. 中国农业科学,2014,47(3):422-430
[20] 孙建,张秀荣,张艳欣,黄波,车卓. 芝麻不同品种(系)耐湿性的综合评价[J]. 中国油料作物学报,2008,30(4):518-521
[21] 孙建,张秀荣,张艳欣,王林海,黄波. 湿害处理对不同生育时期芝麻叶片保护酶活性和种子产量的影响[J]. 应用与环境生物学报,2009,15(6):790-795
[22] 江诗洋,黎冬华,张艳欣,魏鑫,张秀荣,王林海. 水涝胁迫下芝麻内源激素及表型响应[J]. 中国油料作物学报,2015,37(5):676-682
[23] 范月仙,王鹏,李生泉,李锐,李娜,郝辉芳. 临界生长温度锻炼对棉苗抗冷性的影响[J]. 棉花学报,2011,23(1):90-93
[24] 毕颖,刘燕,张平.蒽酮比色法测定大豆乳清废水中可溶性总糖含量[J].大豆通报,2006(3): 24-25
[25] 高京草,王慧霞,李西选. 可溶性蛋白、丙二醛含量与枣树枝条抗寒性的关系研究[J]. 北方园艺,2010(23):18-20
[26] 李忠光,李江鸿,杜朝昆,黄号栋,龚明. 在单一提取系统中同时测定五种植物抗氧化酶[J]. 云南师范大学学报(自然科学版),2002,22(6):44-48
[27] 张蕊,吕俊,米青山,王三根. 低温下外源水杨酸对水稻幼苗抗氧化酶系的影响[J]. 西南农业大学学报(自然科学版),2006,28(1): 29-32
[28] 焦蓉,刘好宝,刘贯山,王树林,侯娜,王全贞,刘朝科,冯祥国,胡晓明,靳义荣. 论脯氨酸累积与植物抗渗透胁迫[J]. 中国农学通报,2011,27(7):216-221
[29] 汪妮. 渍涝胁迫对不同基因型芝麻生理特性及产量性状的影响[D].郑州:河南农业大学,2009
[30] Yoshiba Y, Kiyosue T. Regulation of levels of proline as osmolyte in plants under water stress [J].Plant and Cell Physiology, 1997, 38(10):1095-1102
[31] 段素梅,杨安中,黄义德,吴文革,许有尊,陈刚. 干旱胁迫对水稻生长、生理特性和产量的影响[J]. 核农学报,2014,28(6):1124-1132
[32] Garcia A B, Almeida-Enhler J, Iyer S. Effects of osmoprotectants upon NaCl stress in rice [J].Plant Physiology, 1997, 115(1):159-169
[33] Liu J, Zhu J K. Proline accumulation and salt-stress-induced gene expression in a salt-hypersensitive mutant of Arabidopsis [J]. Plant Physiology, 1997, 114(2):591
[34] 刘娥娥,宗会,郭振飞, 黎用朝. 干旱、盐和低温胁迫对水稻幼苗脯氨酸含量的影响[J]. 热带亚热带植物学报,2000,8(3):235-238
[35] 袁梦雅.干旱胁迫对植物保护酶的影响[J]. 科技信息, 2011 (14):105
[36] 韩轩,蔡萍,伊卫东,韩晓亮,徐振朋,宛诣超. 内蒙古8份冷蒿种质资源材料抗寒性的初步研究[J]. 草地学报,2014,22(3):669-672
[37] 吴敏,张文辉,周建云,马闯,韩文娟. 干旱胁迫对栓皮栎幼苗细根的生长与生理生化指标的影响[J]. 生态学报,2014,34(15):4223-4233
[38] 苗雨晨, 白玲, 苗琛, 陈珈, 宋纯鹏. 植物谷胱甘肽过氧化物酶研究进展[J]. 植物学通报, 2005, 22(3): 350-356
[39] Asada K. Ascorbate peroxidase: a hydrogen peroxide scavenging enzyme in plants [J]. Physiologia Plantarum, 1992, 85(2):235-241
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