胃炎颗粒中3种活性成分的定性鉴别及芍药苷含量测定
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摘要
目的建立胃炎颗粒中3种主要中药成分的定性鉴别和芍药苷含量测定的方法。方法采用薄层色谱(TLC)法对方中黄芪、白芍、白芷进行定性鉴别;采用高效液相色谱法测定芍药苷的含量,色谱柱为Agilent-TC C_(18)柱(250 mm×4. 6 mm,5μm),流动相为乙腈-甲醇-0. 1%磷酸溶液(7∶13∶80),流速为1. 0 m L/min,检测波长为230 nm,柱温为30℃。结果 TLC鉴别法专属性强,色谱斑点清晰,阴性对照无干扰。芍药苷色谱峰与其他色谱峰分离良好,进样质量浓度在20～120μg/m L范围内与峰面积线性关系良好(r=0. 999 9);平均加样回收率为101. 44%,RSD为1. 00%(n=6); 3批次9个样品每10 g中芍药苷的含量平均值为14. 62 mg。结论该方法可用于制剂中黄芪、白芍、白芷的成分鉴别及芍药苷的含量测定。
Objective To establish a method for qualitative identification and content determination of paeoniflorin in Weiyan Granules.Methods TLC method was adopted to qualitatively identify Astragali Radix, Paeoniae Radix Alba and Angelicae Dahuricae Radix in Weiyan Granules,and HPLC method was adopted to determine the content of paeoniflorin in Weiyan Granules,the chromatographic column was Agilent-TC C_(18) column(250 mm × 4. 6 mm,5 μm),the mobile phase was acetonitrile-methanol-0. 1% phosphoric acid(7 ∶ 13 ∶ 80),the flow rate was 1. 0 m L/min,the detection wavelength was 230 nm,and the column temperature was 30 ℃. Results TLC identification method had strong specificity, clear chromatographic spots, and no interference from negative control. The chromatographic peaks of paeoniflorin were well separated from other chromatographic peaks. The linear relationship between the concentration of paeoniflorin and the peak area was good in the range of 20-120 μg/m L( r = 0. 999 9). The average recovery of paeoniflorin was 101. 44%, RSD was1. 00%( n = 6). The average paeoniflorin content of nine samples( three from each batch) was 14. 62 mg/10 g. Conclusion The method can be used for qualitative identification of Astragali Radix, Paeoniae Radix Alba and Angelicae Dahuricae Radix and content determination of paeoniflorin in Weiyan Granules.
Objective To establish a method for qualitative identification and content determination of paeoniflorin in Weiyan Granules.Methods TLC method was adopted to qualitatively identify Astragali Radix, Paeoniae Radix Alba and Angelicae Dahuricae Radix in Weiyan Granules,and HPLC method was adopted to determine the content of paeoniflorin in Weiyan Granules,the chromatographic column was Agilent-TC C_(18) column(250 mm × 4. 6 mm,5 μm),the mobile phase was acetonitrile-methanol-0. 1% phosphoric acid(7 ∶ 13 ∶ 80),the flow rate was 1. 0 m L/min,the detection wavelength was 230 nm,and the column temperature was 30 ℃. Results TLC identification method had strong specificity, clear chromatographic spots, and no interference from negative control. The chromatographic peaks of paeoniflorin were well separated from other chromatographic peaks. The linear relationship between the concentration of paeoniflorin and the peak area was good in the range of 20-120 μg/m L( r = 0. 999 9). The average recovery of paeoniflorin was 101. 44%, RSD was1. 00%( n = 6). The average paeoniflorin content of nine samples( three from each batch) was 14. 62 mg/10 g. Conclusion The method can be used for qualitative identification of Astragali Radix, Paeoniae Radix Alba and Angelicae Dahuricae Radix and content determination of paeoniflorin in Weiyan Granules.
引文
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[16]胡斌,林彩,赵映兰,等.胃炎颗粒质量标准研究[J].中国药业,2009,18(23):28-29.
[17]朱艺欣,李宝莉,马宏胜,等.白芷的有效成分提取、药理作用及临床应用研究进展[J].中国医药导报,2014,11(31):159-166.
[18]徐任生.天然产物药物化学[M].北京:科学出版社,2005:596-597.
[19]周国华,傅小英.医疗机构制剂检验基本操作指南[M].北京:人民军医出版社,2012:73.
[2]周晓明.黄芪建中汤治疗胃溃疡55例[J].中国中医药现代远程教育,2014,12(2):93-94.
[3]崔虹,朱佳茜,冯秋芳,等.中药白芍化学成分及生物活性研究进展[J].海峡药学,2017,29(9):1-5.
[4]张玉杰,李明春,梁延春,等.参芪苓口服液的薄层色谱鉴别[J].云南中医学院学报,2015,38(3):21-24.
[5]孙安武.黄芪薄层色谱法探讨[J].中国中医药现代远程教育,2007,5(8):40-41.
[6]蒋鹏娜,李星,王雪慧,等.平喘颗粒质量标准研究[J].中华中医药杂志,2017,32(5):2132-2136.
[7]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015.
[8]蒋洁莹,刘瑾,李世芳,等.丹芩消郁合剂质量标准研究[J].四川中医,2016,34(3):103-106.
[9]胡婧,易凡力,刘松青.十五味黑药丸中4种活性成分的定性鉴别及芍药苷的含量测定[J].第三军医大学学报,2011,33(6):621-624.
[10]臧凯宏,吴建军,秦红岩,等.黄芪多糖对溃疡性结肠炎大鼠肠道黏膜屏障的影响[J].中药材,2017,40(1):208-211.
[11]赵唯含,毛堂友,杨美娟,等.黄芪、三七及其配伍对慢性萎缩性胃炎大鼠胃黏膜细胞凋亡及增殖的影响[J].中国中西医结合消化杂志,2017,25(5):376-380.
[12]李乃谦.探讨白芍的药理作用及现代研究进展[J].中医临床研究,2017,9(20):137-138.
[13]吴媛媛,蒋桂华,马逾英,等.白芷的药理作用研究进展[J].时珍国医国药,2009,20(3):325-627.
[14]贾福群,刘卫东,任建国.通脉颗粒的质量标准研究[J].西北药学杂志,2017,32(2):130-133.
[15]何风雷,郑华珠,石洪超,等. HPLC法测定胃疡宁丸中芍药苷的含量[J].中国医药导报,2017,14(9):133-135.
[16]胡斌,林彩,赵映兰,等.胃炎颗粒质量标准研究[J].中国药业,2009,18(23):28-29.
[17]朱艺欣,李宝莉,马宏胜,等.白芷的有效成分提取、药理作用及临床应用研究进展[J].中国医药导报,2014,11(31):159-166.
[18]徐任生.天然产物药物化学[M].北京:科学出版社,2005:596-597.
[19]周国华,傅小英.医疗机构制剂检验基本操作指南[M].北京:人民军医出版社,2012:73.
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