一种光照诱导原位产生过氧亚硝酸根的新方法
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摘要
已证实体内过氧亚硝酸根离子与人体多种病理反应相关。采用S-亚硝基硫醇为一氧化氮供体化合物、水溶性优于核黄素的聚乙二醇化核黄素为光敏剂,在光照条件下各自分解出一氧化氮和超氧负离子,并迅速原位转化为过氧亚硝酸根离子。利用酪氨酸和牛血清白蛋白分别作为过氧亚硝酸根离子捕获剂,证实了这是一种体外产生过氧亚硝酸根的有效方法。
Peroxynitrite has been associated with various pathological changes in the body.S-Nitroso alcohol was used as the nitric oxide donor compound,and the PEGylated riboflavin which the water solubility was better than riboflavin as the photosensitizer.Under the light conditions,the nitric oxide and the superoxide anion were decomposed separately.It was rapidly converted into the peroxynitrite ion.The tyrosine and bovine serum albumin were used respectively as peroxynitrite ion capture agent to be proved an effective method for producing peroxynitrite in vitro.
Peroxynitrite has been associated with various pathological changes in the body.S-Nitroso alcohol was used as the nitric oxide donor compound,and the PEGylated riboflavin which the water solubility was better than riboflavin as the photosensitizer.Under the light conditions,the nitric oxide and the superoxide anion were decomposed separately.It was rapidly converted into the peroxynitrite ion.The tyrosine and bovine serum albumin were used respectively as peroxynitrite ion capture agent to be proved an effective method for producing peroxynitrite in vitro.
引文
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[17]SAWA T,AKAIKE T,MAEDA H.Tyrosine nitration by peroxynitrite formed from nitric oxide and superoxide generated by xanthine oxidase[J]. J. Biol. Chem.,2000,275(42):32 467.
[2]WINK D A,COOK J A,PACELLI R,et al. Nitric oxide(NO)protects against cellular damage by reactive oxygen species[J].Toxicol.Lett.,1995,82-83(6):221.
[3]TROSTCHANSKY A,BATTHYANY C,BOTTI H,et al.Formation of lipid-protein adducts in low-density lipoprotein by fluxes of peroxynitrite and its inhibition by nitric oxide[J]. Arch. Biochem. Biophys.,2001,395(2):225-232.
[4]RUBBO H,TROSTCHANSKY A,BOTTI H,et al.Interactions of nitric oxide and peroxynitrite with low-density lipoprotein[J].Biol.Chem.,2002,383(3/4):547-552.
[5]MATSUNATA T,NAKAJIMA T,SONODA M,et al.Modulation of reactive oxygen species in endothelial cells by peroxynitrite-treated lipoproteins[J]J. Biochem.,2001,130(2):285-293.
[6]PENNATHUR S,BERGT C,SHAO B,et al.Human atherosclerotic intima and blood of patients with established coronary artery disease contain high density lipoprotein damaged by reactive nitrogen species[J]. J. Biochem.,2004,279(41):42 977-42 983.
[7]SHAO B,BERGT C,FU X,et al.Tyrosine 192 in apolipoprotein A-I is the major site of nitration and chlorination by myeloperoxidase,but only chlorination markedly impairs ABCA1-dependent cholesterol transport[J]. J.Biochem.,2005,280(7):5 983-5 993.
[8]CERIELLO A,MERCURI F,QUAGLIARO L,et al. Detection of nitrotyrosine in the diabetic plasma:evidence of oxidative stress[J].Diabetologia,2001,44(7):834-838.
[9]DELANEY C A,TYRBERG B,BOUWENS L,et al.Sensitivity of human pancreatic islets to peroxynitrite-induced cell dysfunction and death[J].Febs Lett.,1996,394(3):300-306.
[10]REYNOLDS M R,LUKAS T J,BERRY R W,et al.Peroxynitrite-mediated tau modifications stabilize preformed filaments and destabilize microtubules through distinct mechanisms[J].Biochemistry,2006,45(13):4 314.
[11]苏江涛,张登峰,宋鑫,等.新族亚硝基硫醇分子的设计合成及NO释放稳定性研究[J].分子科学学报,2016,32(1):71-77.
[12]陈宇,孟德胜.S-亚硝基-N-乙酰基-DL-青霉胺的化学合成及其缓释片的试制备[J].重庆医学,2003,32(4):471-472.
[13]WANG J,SU B,XING H,et al.Preparation and characterization of mono-and di-d-α-tocopheryl polyethylene glycol 1000 succinate[J]. J. Appl. Polym. Sci.,2010,119(5):3 026-3 033.
[14]ROMERO-PUERTAS M C,RODRIGUEZ-SERRANO M,CORPAS F J,et al.Cadmium-induced subcellular accumulation of O-2,and H2O2,in pea leaves[J]. Plant Cell Environ.,2004,27(9):1 122-1 134.
[15]MCANINLY J,WILLIAMS D L H,ASKEW S C,et al.Metal ion catalysis in nitrosothiol(RSNO)decomposition[J]. J. Chem. Soc. Chem. Commun.,1993,23(23):1 758-1 759.
[16]闫莉,许言午,燕子,等.3-硝基酪氨酸标准品的制备[J].中国药物与临床,2006,6(9):653-654.
[17]SAWA T,AKAIKE T,MAEDA H.Tyrosine nitration by peroxynitrite formed from nitric oxide and superoxide generated by xanthine oxidase[J]. J. Biol. Chem.,2000,275(42):32 467.