濒危物种雅连及其不同炮制品对2型糖尿病大鼠的降糖调脂药效差异研究
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摘要
目的考察黄连濒危物种雅连Coptis deltoidea在传统加工前后,对2型糖尿病大鼠血糖、血脂的作用差异。方法采用高糖、高脂饮食复合小剂量链脲佐菌素(STZ)诱导的方法复制大鼠2型糖尿病模型,考察雅连分别采用黄酒、生姜和吴茱萸炮制后,与生品相比,对模型大鼠空腹血糖(FBG)、空腹血清胰岛素(FINS)、糖化血清蛋白(GSP)、血清三酰甘油(TG)、总胆固醇(TC)、脂蛋白(HDL-C/LDL-C)水平,以及SCAP/SREBP-1c固醇调节通路关键蛋白及其基因表达的药效及差异。结果在降糖方面,雅连及其炮制品均能降低模型大鼠FBG及GSP水平,修复受损胰腺,但雅连经黄酒和吴茱萸炮制后,对FBG及GSP降低作用更为显著(P<0.01),其中以酒炙品降糖幅度最大;在调脂方面,雅连及其炮制品均能够降低模型大鼠血清TG、TC、LDL-C水平(P<0.05、0.01),提高HDL-C水平(P<0.05、0.01),并下调胰岛素靶器官肝脏SCAP、SREBP-1c蛋白及其基因表达(P<0.05、0.01),但酒炙及萸炙品优于姜炙品或雅连生品。结论用黄酒、生姜、吴茱萸对雅连进行炮制均为改善雅连本性苦寒不易久服弊端的传统方法,但不同炮制方式对雅连药效有不同影响,临床用药时应辨证施治,单纯降糖选用雅连酒炙品为宜;而降糖调脂综合药效则以酒炙及萸炙品更优。
Objective To investigate the difference of hypoglycemic and hypolipidemic effects of Coptis deltoidea(Coptis chinensis) and its processed products on T2DM rats. Methods Type 2 diabetes mellitus induced by high sugar, high fat diet and low dose of streptozotocin. C. deltoidea were processed with yellow wine, ginger, and evodia rutaecarpa, respectively. The effects of C. deltoideaon fasting blood glucose(FBG), fasting insulin(FINS), glycosylated serum protein(GSP), pancreatic pathology, serum triglyceride(TG), total cholesterol(TC) and lipoprotein(HDL-C or LDL-C) and key protein and gene expression of SCAP\SREBP-1c pathway in liver of model rats were investigated. Result C. deltoidea and its processed products reduced the FBG and GSP in T2DM rats. The wine products significantly reduced FBG and GSP in model rats(P < 0.01). The hypoglycemic range was better than raw and ginger products. At hypolipidemic aspect, C. deltoidea and its processed products decreased the content of TG, TC, and LDL-C(P < 0.05, 0.01), increased the content of HDL-C in serum(P < 0.05, 0.01), and down-regulated the protein and gene expression of SCAP/SREBP-1c in liver, the insulin target organ. However, wine and evodia products were better than crude drug or ginger products. Conclusion C. deltoidea processed by yellow wine, ginger and evodia rutaecarpa is traditional method to improve the disadvantages of C. deltoidea with bitter cold nature and not easy to take for a long time. However, different processing methods have different effects. Clinical medication should be combied withTCM syndrome differentiation and simple hypoglycemic effect of wine product is appropriate; while wine and evodia products were better in hypoglycemic and lipid regulation aspects.
Objective To investigate the difference of hypoglycemic and hypolipidemic effects of Coptis deltoidea(Coptis chinensis) and its processed products on T2DM rats. Methods Type 2 diabetes mellitus induced by high sugar, high fat diet and low dose of streptozotocin. C. deltoidea were processed with yellow wine, ginger, and evodia rutaecarpa, respectively. The effects of C. deltoideaon fasting blood glucose(FBG), fasting insulin(FINS), glycosylated serum protein(GSP), pancreatic pathology, serum triglyceride(TG), total cholesterol(TC) and lipoprotein(HDL-C or LDL-C) and key protein and gene expression of SCAP\SREBP-1c pathway in liver of model rats were investigated. Result C. deltoidea and its processed products reduced the FBG and GSP in T2DM rats. The wine products significantly reduced FBG and GSP in model rats(P < 0.01). The hypoglycemic range was better than raw and ginger products. At hypolipidemic aspect, C. deltoidea and its processed products decreased the content of TG, TC, and LDL-C(P < 0.05, 0.01), increased the content of HDL-C in serum(P < 0.05, 0.01), and down-regulated the protein and gene expression of SCAP/SREBP-1c in liver, the insulin target organ. However, wine and evodia products were better than crude drug or ginger products. Conclusion C. deltoidea processed by yellow wine, ginger and evodia rutaecarpa is traditional method to improve the disadvantages of C. deltoidea with bitter cold nature and not easy to take for a long time. However, different processing methods have different effects. Clinical medication should be combied withTCM syndrome differentiation and simple hypoglycemic effect of wine product is appropriate; while wine and evodia products were better in hypoglycemic and lipid regulation aspects.
引文
[1]耿耘,蒋合众,林琳,等.峨眉产黄连质量控制的研究[J].河北医药, 2009, 31(23):3298-3299.
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[6]刘睿颖,任瑶瑶,张思远,等.味连与濒危物种雅连改善T2DM大鼠糖、脂代谢紊乱的研究[J].华西药学杂志, 2018, 33(4):368-372.
[7]刘睿颖,张思远,任彬,等.从SCAP/SREBP-1c通路探究味连及雅连对2型糖尿病大鼠的降脂作用[J].中国中药杂志, 2018, 43(9):36-40.
[8]廖庆文,樊冬丽,肖小河,等.不同黄连炮制品HPLC指纹图谱研究[J].中国中药杂志, 2007, 32(2):210-214.
[9]降雪,张凡,赵佳丽,等.黄连酒炙过程中5种生物碱的变化研究[J].中国中医药信息杂志, 2011, 18(4):51-53.
[10]彭绵,马艳芬,陈澍.脂质代谢紊乱与胰岛素抵抗相关性研究进展[J].国外医学, 2004, 25(6):278-280.
[11]吕继宏.脂代谢紊乱与T2DM胰岛素分泌及胰岛素抵抗的关系探讨[D].延安:延安大学, 2014.
[12]邓雅婷,廖琼峰,毕开顺,等.黄连-吴茱萸药对化学成分的HPCL-DAD-MS分析[J].药学学报, 2008, 43(3):299-302.
[2]曾军.行将消失的瑰宝——亟待拯救的峨眉雅连情况调查报告[J].中国食品药品监管, 2013, 10(15):56-57.
[3]时晓缇.川产黄连及其炮制品降糖化学成分与药效研究[D].成都:西南交通大学, 2017.
[4]穆松牛,高云,万福生,等.链尿佐菌素加高糖高脂饮食复制大鼠2型糖尿病模型[J].中国比较医学杂志,2008, 18(2):19-21.
[5]穆松牛,康路妹,谢彦海.大鼠2型糖尿病动物模型的建立[J].江西医学院学报, 2009, 49(8):16-19.
[6]刘睿颖,任瑶瑶,张思远,等.味连与濒危物种雅连改善T2DM大鼠糖、脂代谢紊乱的研究[J].华西药学杂志, 2018, 33(4):368-372.
[7]刘睿颖,张思远,任彬,等.从SCAP/SREBP-1c通路探究味连及雅连对2型糖尿病大鼠的降脂作用[J].中国中药杂志, 2018, 43(9):36-40.
[8]廖庆文,樊冬丽,肖小河,等.不同黄连炮制品HPLC指纹图谱研究[J].中国中药杂志, 2007, 32(2):210-214.
[9]降雪,张凡,赵佳丽,等.黄连酒炙过程中5种生物碱的变化研究[J].中国中医药信息杂志, 2011, 18(4):51-53.
[10]彭绵,马艳芬,陈澍.脂质代谢紊乱与胰岛素抵抗相关性研究进展[J].国外医学, 2004, 25(6):278-280.
[11]吕继宏.脂代谢紊乱与T2DM胰岛素分泌及胰岛素抵抗的关系探讨[D].延安:延安大学, 2014.
[12]邓雅婷,廖琼峰,毕开顺,等.黄连-吴茱萸药对化学成分的HPCL-DAD-MS分析[J].药学学报, 2008, 43(3):299-302.
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