丙烯基硫脲和钼酸钠对紫外分光光度法和酚二磺酸光度法测定硝态氮的影响
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摘要
引入抑制剂是研究自然界氮循环的常用手段,然而抑制剂的存在可能会对某些指标(如硝态氮)的测定产生不利影响。实验结果表明,紫外分光光度法测定硝态氮的结果易受干扰物质(抑制剂)的影响,其中丙烯基硫脲(ATU)对于硝态氮测定结果的影响难以校正或消除,钼酸钠在220nm波长处的光谱吸收峰对硝态氮的吸收峰产生了覆盖,导致其测定结果偏差大;酚二磺酸光度法不易受干扰物质影响,在丙烯基硫脲或钼酸钠存在时测定结果稳定可信,可作为硝态氮测定方法使用。
The introduction of inhibitors is a common method in studying nitrogen cycling,and the presence of inhibitors may have adverse effects on the determination of certain indicators,e.g.nitrate nitrogen.Results showed that ultraviolet spectrophotometry is vulnerable to extraneous interference.The influence of allylthiourea(ATU)on the determination of nitrate nitrogen is difficult to correct or eliminate,and the spectral absorption peak of sodium molybdate at 220 nm overlapped with the absorption peak of nitrate nitrogen,thus leading to a great deviation of the latter results.In contrast,nitrate-spectrophotometric method with penol disulonic acid is not easily disturbed by extraneous substances and the results are stable and reliable with the introduction of allyl thiourea or sodium molybdate,respectively.Therefore,nitratespectrophotometric method with penol disulonic acid can be used as a reliable method for nitrate nitrogen determination.
The introduction of inhibitors is a common method in studying nitrogen cycling,and the presence of inhibitors may have adverse effects on the determination of certain indicators,e.g.nitrate nitrogen.Results showed that ultraviolet spectrophotometry is vulnerable to extraneous interference.The influence of allylthiourea(ATU)on the determination of nitrate nitrogen is difficult to correct or eliminate,and the spectral absorption peak of sodium molybdate at 220 nm overlapped with the absorption peak of nitrate nitrogen,thus leading to a great deviation of the latter results.In contrast,nitrate-spectrophotometric method with penol disulonic acid is not easily disturbed by extraneous substances and the results are stable and reliable with the introduction of allyl thiourea or sodium molybdate,respectively.Therefore,nitratespectrophotometric method with penol disulonic acid can be used as a reliable method for nitrate nitrogen determination.
引文
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[3]Wang B.Study of Inhibiting the SRB’s Activity Using the Ecological Technigues[D].Harbin:Harbin Institute of Technology,2008(in Chinese).(王博.生态抑制硫酸盐还原菌活性研究[D].哈尔滨:哈尔滨工业大学,2008.)
[4]Smith R L,Klug M J.Electron Donors Utilized by Sulfate-reducing Bacteria in Eutrophic Lake Sediments[J].Appl Microbiol Biotechnol,1981,42(1):116-121.
[5]State Environmental Protection Administration of China,Editorial Board of Water and Wastewater Monitoring and Analysis Methods.Method for Monitoring and Analyzing Water and Wastewater(Fourth Edition)[M].Beijing:China Environmental Science Press,2002(in Chinese).(国家环境保护总局,水和废水监测分析方法编委会.水和废水监测分析方法(第4版)[M].北京:中国环境科学出版社,2002.)
[6]Zhang Y,Zhang S,Liu C L,et al.Effect of Nitrification Inhibitor of Dicyandiamide on the Determination of NO-3-N in Soil[J].J Hebei Agric Sci,2010,(10):161-164(in Chinese).(张云,张胜,刘长礼,等.双氰胺抑制剂对土壤硝态氮测定的影响[J].河北农业科学,2010,(10):161-164.)
[7]Guo Z Z,Deng F Y,Yong F Z.Photometric Determination of Total Nitrogen in Water with Phenol Disulfonic Acid as Color Reagent[J].PTCA(Part B:Chem Anal),2009,(05):592-593(in Chinese).(郭姿珠,邓飞跃,雍伏曾.酚二磺酸光度法测定水中总氮[J].理化检验(化学分册),2009,(05):592-593.)
[8]Shu L.Determination of Total Nitrogen in Surface Water by Phenol Two Sulfonic Acid Colorimetry[J].Henan Geol,1998,(03):67(in Chinese).(舒兰.用酚二磺酸比色法测定地表水中总氮[J].河南地质,1998,(03):67.)
[9]Guo Z Z,Deng F Y,Yong F Z.Improvement of Determination Method of Total Nitrogen in Water[J].Chin Water Wastewater,2007,22(23):82-84(in Chinese).(郭姿珠,邓飞跃,雍伏曾.水中总氮测定方法的改进[J].中国给水排水,2007,22(23):82-84.)
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