聚酰胺分离纯化金花葵花总黄酮的工艺研究
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摘要
利用聚酰胺柱层析对金花葵花总黄酮的分离纯化条件进行了研究。以金花葵花总黄酮质量浓度为指标,通过考察聚酰胺树脂对黄酮的吸附和解吸能力,筛选出聚酰胺柱层析分离纯化金花葵花总黄酮的最优参数。结果表明,当金花葵花总黄酮的上样液质量浓度为4.68 mg/mL、pH为4～5,在1.5 mL/min的吸附流速下上样1.5 BV时,可达到吸附饱和。接下来用3.0 BV水淋洗去杂质,再用5.0 BV体积分数为80%乙醇解吸液,在1.5 mL/min的解吸流速下进行解吸,树脂每使用5次需要进行一次再生处理,此时分离纯化效果最佳。该研究结果为金花葵花的深入开发和应用提供了依据。
The separation and purification conditions of total flavonoids from Flos Lonicerae were studied by polyamide column chromatography. Based on the concentration of the total flavonoids, by the way of the adsorption and desorption abilities of polyamide resin, the optimum parameters of the separation and purification of the total flavonoids of Hibiscus manihot L are selected and evaluated. The main results are as follows: Purification conditions are as follows: the concentration of sample solution is 4.68 mg/mL,the pH is 4～5, adsorption rare is 1.5 mL/min, sample volume is 1.5 BV, water washing resin column with 3.0BV, the elution rate is 1.5 mL/min with 5.0 BV 80% ethanol has a best effect with polyamides for five times. The result provided a basis for further development andapplication of Hibiscus manihot L.
The separation and purification conditions of total flavonoids from Flos Lonicerae were studied by polyamide column chromatography. Based on the concentration of the total flavonoids, by the way of the adsorption and desorption abilities of polyamide resin, the optimum parameters of the separation and purification of the total flavonoids of Hibiscus manihot L are selected and evaluated. The main results are as follows: Purification conditions are as follows: the concentration of sample solution is 4.68 mg/mL,the pH is 4～5, adsorption rare is 1.5 mL/min, sample volume is 1.5 BV, water washing resin column with 3.0BV, the elution rate is 1.5 mL/min with 5.0 BV 80% ethanol has a best effect with polyamides for five times. The result provided a basis for further development andapplication of Hibiscus manihot L.
引文
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[3]刘琦,辛秀兰,兰蓉,等.金花葵花中总黄酮提取工艺的研究[J].食品研究与开发,2011,32(7):19-21.
[4]徐玮.茶多酚对幼犬生长、免疫、抗氧化性能及脂类代谢的影响[D].青岛:青岛农业大学,2009.
[5]文开新,王成章,严学兵,等.黄酮类化合物生物学活性研究进展[J].草业科学,2010,27(6):115-122.
[6] Wan P F,Sheng Z L,Han Q,et al. Enrichment and purification of total flavonoids from Flos Populi extracts with macroporous resins and evaluation of antioxidant activities in vitio[J]. Journal of Chromatography B Analytical Technologies in the Biomedical and Life Science,2014,945-946(2):68-74.
[7] Chen J,Li Y,Yang L Q,et al. Biological activities of flavonoids from pathogenic-infected Astragalus adsurgens[J]. Food Chemistry,2012,131(2):546-551.
[8]王庆明,张金生,李丽华,等.微波萃取—分光光度法测定党参中黄酮的研究[J].辽宁石油化工大学学报,2010,30(2):8-10.
[9]王菲.软枣猕猴桃黄酮类化合物的提取纯化及生物活性研究[D].沈阳:沈阳农业大学,2011.
[10]王琳,朱缨,吴纪凯.参银复智制剂中银杏内酯的提取分离检测[J].中国药业,2014,23(13):36-38.
[11]岳琳娜.绞股蓝皂苷提取及分离纯化工艺的研究[D].合肥:安徽农业大学,2009.
[12]张玉,吴慧明,余建伟,等.聚酰胺分离纯化柑桔皮中总黄酮的工艺研究[J].食品工业科技,2007,28(11):147-149.
[13]刘长江,栾云峰,王菲,等.大孔吸附树脂分离纯化软枣猕猴桃总黄酮[J].食品科学,2011,32(12):145-149.
[14]刘志祥,曾超珍,张玥. AB-8大孔树脂分离纯化枸骨叶总黄酮的工艺研究[J].食品科学,2010,31(12):76-79.
[15]鄢又玉.火棘果活性成分分析制备及其制剂基质研究[D].武汉:华中科技大学,2016.
[16]卫建琮,李华峰,乔华,等.中药蒲公英花中总黄酮的分离纯化研究[J].中国药物与临床,2014,14(1):19-21.
[17]李秋哲.莲子心黄酮结构分析及其降血糖活性研究[D].福州:福建农林大学,2015.