云南药用野生稻对四种水稻主要病害的抗性鉴定
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摘要
对云南药用野生稻7个不同居群的稻瘟病、纹枯病、白叶枯病和细菌性条斑病抗型表型进行鉴定,筛选抗源,并检测已克隆的抗稻瘟病、抗白叶枯病基因在它们中的存在情况。结果表明:用云南稻瘟病菌毒性菌株16t接种云南药用野生稻7个居群,除勐海药野(7)表型为感病外,其他6个居群均为抗病;勐遮药用野生稻(4)和景纳上沟药用野生稻(1)分别不含Pib和Pi2基因片段,其他5个居群都含有Pib、Pi2、Pi9、Pid2、Pikp、Pis和Pi56等基因片段;所有的参试药用野生稻高抗纹枯病;除勐往药野(13)和澜沧孟矿药野(14)对细菌性条斑病菌菌株RS105表现为感病和勐遮药野(5)对菌株RS1-20表现为感病外,其他材料对菌株RS105和RS1-20都表现为抗病;云南白叶枯病菌强致病性菌株CX30-1、菲律宾菌株PXO99和PXO86对景纳上沟药用野生稻(1)和澜沧孟矿药用野生稻(14)具有强致病性,其他居群表现为抗病至中抗; 7个居群都含有Xa5、Xa13、Xa21基因片段。本文首次报道了云南药用野生稻多个居群类型对4种主要病害的抗性,这些结果为深度挖掘利用云南药用野生稻资源的有效抗病基因奠定了基础。
Phenotypic characteristics of resistance to rice blast,sheath blight,bacterial blight,and bacterial leaf streak of seven different populations of Oryza offcinalis from Yunnan Province were identified. Detection of the presence of cloned resistant genes of rice blast and bacterial blight was carried out. The results showed that six O.offcinalis populations except Menghai( 7) had resistance to rice blast. The Mengzhe( 4) and Jingnashanggou( 1) did not contain Pib and Pi2,respectively,and all of other five O. offcinalis populations contained Pib,Pi2,Pi9,Pid2,Pikp,Pis,and Pi56. All tested O. offcinalis populations showed high resistance to sheath blight and bacterial leaf streak isolates RS105 and RS1-20,except Mengzhe( 5) was susceptible to isolate RS1-20,Mengwang( 13) and Lancangmengkuang( 14) was susceptible to isolate RS105. Strong virulent isolate of Yunnan Xoo CX30-1 and Philippines Xoo isolates PXO99 and PXO86 had strong pathogenicity to Jingnashanggou( 1)and Lancangmengkuang( 14),but other wild rice populations showed resistance or moderate resistance to these three isolates. Seven O. offcinalis populations contained Xa5,Xa13,and Xa21 genes. This study is the first report that O. offcinalis populations of Yunnan had different characteristics of resistance to four diseases,which will be beneficial to laying foundation for deeply digging resistance genes from O. offcinalis in Yunnan Province.
Phenotypic characteristics of resistance to rice blast,sheath blight,bacterial blight,and bacterial leaf streak of seven different populations of Oryza offcinalis from Yunnan Province were identified. Detection of the presence of cloned resistant genes of rice blast and bacterial blight was carried out. The results showed that six O.offcinalis populations except Menghai( 7) had resistance to rice blast. The Mengzhe( 4) and Jingnashanggou( 1) did not contain Pib and Pi2,respectively,and all of other five O. offcinalis populations contained Pib,Pi2,Pi9,Pid2,Pikp,Pis,and Pi56. All tested O. offcinalis populations showed high resistance to sheath blight and bacterial leaf streak isolates RS105 and RS1-20,except Mengzhe( 5) was susceptible to isolate RS1-20,Mengwang( 13) and Lancangmengkuang( 14) was susceptible to isolate RS105. Strong virulent isolate of Yunnan Xoo CX30-1 and Philippines Xoo isolates PXO99 and PXO86 had strong pathogenicity to Jingnashanggou( 1)and Lancangmengkuang( 14),but other wild rice populations showed resistance or moderate resistance to these three isolates. Seven O. offcinalis populations contained Xa5,Xa13,and Xa21 genes. This study is the first report that O. offcinalis populations of Yunnan had different characteristics of resistance to four diseases,which will be beneficial to laying foundation for deeply digging resistance genes from O. offcinalis in Yunnan Province.
引文
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[2]National Agricultural Technology Extension Center.Major crop diseases and pests occurred in serious situation in China in 2012(in Chinese)[J].Pesticide Market Information(农药市场信息),2012(5):49.
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[7]Xu M R,Zhou Y L,Li Z K.Research advances in on resistance genetics of rice bacterial blight and bacterial leaf streak(in Chinese)[J].Journal of Plant Genetic Resources(植物遗传资源学报),2012,13(3):370-375.
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[10]Li D Q,Chen L,Li W J,et al.Identification of bacterial blight resistance gene in Yunnan wild rice(in Chinese)[J].Acta Agronomica Sinica(作物学报),2015,41(3):386-393.
[11]Li Q,Luo S X,Huang R Q,et al.Resistance to brown planthopper of Oryza officinalis Wall.from Guangxi[A].Study on the selection of wild rice resources(in Chinese)[M].Beijing:China Science and Technology Press(北京:中国科学技术出版社),1990:49-50.
[12]Huang D H,Cen Z L,Liu C,et al.Identification and genetic analysis of resistance to bacterial leaf streak in wild rice[J].Journal of Plant Genetic Resources,2008,9(1):11-14.
[13]Peng S Q,Wei Z S,Mao C X,et al.Identification of multiresistance of O.meyeriana,O.officinalis and O.sativa f.spantanea growing in Yunnan province(in Chinese)[J].Acta Phytopathologica Sinica(植物病理学报),1982,17(4):58-60.
[14]Eizenga G C,Lee F N,Rutger J N.Screening Oryza species plants for rice sheath blight resistance[J].Plant Disease,2002,86:808-812.
[15]Prasad B,Eizenga G C.Rice sheath blight disease resistance identified in Oryza spp.accessions[J].Plant Disease,2008,92:1503-1509.
[16]Hutin M,Sabot F,Ghesquière A,et al.A knowledge-based molecular screen uncovers a broad-spectrum OsSWEET14 resistance allele to bacterial blight from wild rice[J].Plant Journal,2015,84(4):694-703.
[17]Zeng Y X,Li X M,Ma L Y,et al.Research progress on mapping of gene conferring resistance to sheath blight and exploitation of resistance resources in rice(in Chinese)[J].Chinese Journal Rice Science(中国水稻科学),2010,24(5):544-550.
[18]Zuo S M,Zhang Y F,Yin Y J,et al.Establishment and improvement of inoculation technique and rating system in researching rice sheath blight resistance in field(in Chinese)[J].Journal of Yangzhou University(Agricultural and Life Science Edition)[扬州大学学报(农业与生命科学版)],2006,27(4):57-62.
[19]Lan Y R,Wang J Y,Wang Y,et al.Improvement of rice bacterial blight resistance of Hua 201S,an elite photo-thermo-sensitive genic male sterile line,by molecular marker-assisted selection(in Chinese)[J].Chinese Journal Rice Science(中国水稻科学),2012,25(2):169-174.
[20]Nong X M,Liao H D,Liu Z M,et al.Preliminary report on variation in pathogenicity of pathogen of bacterial streak in Guangxi province(in Chinese)[J].Southwest China Journal of Agricultural Sciences(西南农业学报),1991,4:94-98.
[21]Murray M G,Thompson W F.Rapid isolation of high molecular weight plant DNA[J].Nucleic Acids Research,1980,8:4321-4325.
[22]Zhang P S,Zhao C D,Yu N,et al.Recent progress on cloning and application of rice blast resistance genes(in Chinese)[J].China Rice(中国稻米),2014,20(5):1-7.
[23]Liang B,Xiao F H,Huang F Y,et al.Resistance assessment of Yunnan wild rices to blast(in Chinese)[J].Chinese Journal Rice Science(中国水稻科学),1999,13(3):183-185.
[24]Devanna N B,Vijayan J,Sharma T R.The blast resistance gene Pi54 of cloned from Oryza officinalis interacts with Avr-Pi54 through its novel non-LRR domains[J].PLoS ONE,2014,9(8):e104840.
[25]Huang C L,Hwang S Y,Chiang Y C,et al.Molecular evolution of the Pi-ta gene resistant to rice blast in wild rice(Oryza rufipogon)[J].Genetics,2008,179(3):1527-1538.
[26]Liu J M,Cheng Z Q,Yang M Z,et al.Cloning and sequence analysis of disease resistance gene analogues from three wild rice species in Yunnan(in Chinese)[J].Scientia Agricultura Sinica(中国农业科学),2003,36(3):273-280.
[27]Bao Y,Xu S,Jing X,et al.De Novo assembly and characterization of Oryza officinalis leaf transcriptome by using RNA-seq[J].BioMed Research International,2015:982065.
[28]Qian J,Cheng Z Q,Yang M Z,et al.Isolation and sequence analysis of the Xa21 gene exon II homologs from different species of wild rice in Yunnan(in Chinese)[J].Hereditas(遗传),2005,27:382-386.
[29]Wang G L,Wu C,Zeng L,et al.Isolation and characterization of rice mutants compromised in Xa21-mediated resistance to Xanthomonas oryzae pv.oryzae[J].Theoretical and Applied Genetics,2004,108:379-384.
[2]National Agricultural Technology Extension Center.Major crop diseases and pests occurred in serious situation in China in 2012(in Chinese)[J].Pesticide Market Information(农药市场信息),2012(5):49.
[3]Qian J,Zhang S G,Ji G H.Evaluation of resistance to bacterial blight(Xanthomnas oryzae pv.oryzae)in Yunnan rice cultivars(in Chinese)[J].Journal of Yunnan Agricultural University(云南农业大学学报),2003,18(3):239-246.
[4]Zhang Q.Genetics and improvement of resistance to bacterial blight in hybrid rice in China(in Chinese)[J].Chinese Journal Rice Science(中国水稻科学),2009,23(2):111-119.
[5]Oliudo N,Ronald P C,Bogdanove A J.Xanthomonas oryzae pathovars:model pathogens of a model crop[J].Molecular Plant Pathology,2006,7(5):303-324.
[6]Lee F N,Rush M C.Rice sheath blight:a major rice disease[J].Plant Disease,1983,67:829-832.
[7]Xu M R,Zhou Y L,Li Z K.Research advances in on resistance genetics of rice bacterial blight and bacterial leaf streak(in Chinese)[J].Journal of Plant Genetic Resources(植物遗传资源学报),2012,13(3):370-375.
[8]Wei Y P,Huang D H,Chen Y Z,et al.Identification and evaluation of resistance to rice blast in Guangxi wild rice resources(in Chinese)[J].Chinese Journal Rice Science(中国水稻科学),2009,23(4):433-436.
[9]He G C.Combination of cell engineering and molecular biology an effective approach to the utilization of excellent germplasm resources of wild rice(in Chinese)[J].Progress in Bioengineering(生物工程进展),1998,18(2):41-45.
[10]Li D Q,Chen L,Li W J,et al.Identification of bacterial blight resistance gene in Yunnan wild rice(in Chinese)[J].Acta Agronomica Sinica(作物学报),2015,41(3):386-393.
[11]Li Q,Luo S X,Huang R Q,et al.Resistance to brown planthopper of Oryza officinalis Wall.from Guangxi[A].Study on the selection of wild rice resources(in Chinese)[M].Beijing:China Science and Technology Press(北京:中国科学技术出版社),1990:49-50.
[12]Huang D H,Cen Z L,Liu C,et al.Identification and genetic analysis of resistance to bacterial leaf streak in wild rice[J].Journal of Plant Genetic Resources,2008,9(1):11-14.
[13]Peng S Q,Wei Z S,Mao C X,et al.Identification of multiresistance of O.meyeriana,O.officinalis and O.sativa f.spantanea growing in Yunnan province(in Chinese)[J].Acta Phytopathologica Sinica(植物病理学报),1982,17(4):58-60.
[14]Eizenga G C,Lee F N,Rutger J N.Screening Oryza species plants for rice sheath blight resistance[J].Plant Disease,2002,86:808-812.
[15]Prasad B,Eizenga G C.Rice sheath blight disease resistance identified in Oryza spp.accessions[J].Plant Disease,2008,92:1503-1509.
[16]Hutin M,Sabot F,Ghesquière A,et al.A knowledge-based molecular screen uncovers a broad-spectrum OsSWEET14 resistance allele to bacterial blight from wild rice[J].Plant Journal,2015,84(4):694-703.
[17]Zeng Y X,Li X M,Ma L Y,et al.Research progress on mapping of gene conferring resistance to sheath blight and exploitation of resistance resources in rice(in Chinese)[J].Chinese Journal Rice Science(中国水稻科学),2010,24(5):544-550.
[18]Zuo S M,Zhang Y F,Yin Y J,et al.Establishment and improvement of inoculation technique and rating system in researching rice sheath blight resistance in field(in Chinese)[J].Journal of Yangzhou University(Agricultural and Life Science Edition)[扬州大学学报(农业与生命科学版)],2006,27(4):57-62.
[19]Lan Y R,Wang J Y,Wang Y,et al.Improvement of rice bacterial blight resistance of Hua 201S,an elite photo-thermo-sensitive genic male sterile line,by molecular marker-assisted selection(in Chinese)[J].Chinese Journal Rice Science(中国水稻科学),2012,25(2):169-174.
[20]Nong X M,Liao H D,Liu Z M,et al.Preliminary report on variation in pathogenicity of pathogen of bacterial streak in Guangxi province(in Chinese)[J].Southwest China Journal of Agricultural Sciences(西南农业学报),1991,4:94-98.
[21]Murray M G,Thompson W F.Rapid isolation of high molecular weight plant DNA[J].Nucleic Acids Research,1980,8:4321-4325.
[22]Zhang P S,Zhao C D,Yu N,et al.Recent progress on cloning and application of rice blast resistance genes(in Chinese)[J].China Rice(中国稻米),2014,20(5):1-7.
[23]Liang B,Xiao F H,Huang F Y,et al.Resistance assessment of Yunnan wild rices to blast(in Chinese)[J].Chinese Journal Rice Science(中国水稻科学),1999,13(3):183-185.
[24]Devanna N B,Vijayan J,Sharma T R.The blast resistance gene Pi54 of cloned from Oryza officinalis interacts with Avr-Pi54 through its novel non-LRR domains[J].PLoS ONE,2014,9(8):e104840.
[25]Huang C L,Hwang S Y,Chiang Y C,et al.Molecular evolution of the Pi-ta gene resistant to rice blast in wild rice(Oryza rufipogon)[J].Genetics,2008,179(3):1527-1538.
[26]Liu J M,Cheng Z Q,Yang M Z,et al.Cloning and sequence analysis of disease resistance gene analogues from three wild rice species in Yunnan(in Chinese)[J].Scientia Agricultura Sinica(中国农业科学),2003,36(3):273-280.
[27]Bao Y,Xu S,Jing X,et al.De Novo assembly and characterization of Oryza officinalis leaf transcriptome by using RNA-seq[J].BioMed Research International,2015:982065.
[28]Qian J,Cheng Z Q,Yang M Z,et al.Isolation and sequence analysis of the Xa21 gene exon II homologs from different species of wild rice in Yunnan(in Chinese)[J].Hereditas(遗传),2005,27:382-386.
[29]Wang G L,Wu C,Zeng L,et al.Isolation and characterization of rice mutants compromised in Xa21-mediated resistance to Xanthomonas oryzae pv.oryzae[J].Theoretical and Applied Genetics,2004,108:379-384.
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