西农979中长穗偃麦草(Thinopyrum ponticum)的遗传成分分析
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摘要
西农979是我国黄淮麦区优质高产、早熟耐寒兼抗赤霉病的小麦新品种。十倍体长穗偃麦草(Thinopyrum ponticum)7E染色体携带有抗赤霉病和抗叶锈病等多种抗性基因。为明确西农979品种的遗传基础,以西农979及其主要供体亲本小偃6号、早优504、陕229、陕213和西农881为材料进行系谱分析,结果表明,西农979及其主要供体亲本陕229、陕213、西农881均为小偃6号的衍生系;小偃6号是十倍体长穗偃麦草的衍生系。在此基础上,利用十倍体长穗偃麦草7E染色体上106个特异分子标记进行分析,发现有6个标记在西农979和小偃6号中扩增出了十倍体长穗偃麦草的特异片段,西农979和小偃6号均携带有十倍体长穗偃麦草7E染色体短臂上分子标记Xwmc653-Xwmc809之间的75.10～77.46cM区段,标记Xcfd31-Xgwm350之间的86.16～87.32cM区段,以及7E染色体长臂标记Xmag1932-Xdauk144之间的147.71～149.51cM区段。结果表明,西农979携带的十倍体长穗偃麦草7E染色体上的遗传物质源自小偃6号,这为进一步研究和利用西农979提供了理论参考。
Xinong 979 is a famous facultative cultivar with improved quality, high yield potential, and multi-resistanceto various diseases, and has been widely extended in the southern Huanghuai Wheat Zone in China. Chromosome 7 Eof Thinopyrum ponticum carries Fusarium head blight(FHB) resistance gene(Fhb7), leaf rust resistance gene(Lr19)and stem rust resistance gene(Sr25). In this study, the pedigree and 7 E chromosome molecular marker of Th. ponticumwere used to analyze the genetic relationship among Xinong 979 and its 5 donor parents(Xiaoyan 6, Zaoyou 504,Shaan 229, Shaan 213, and Xinong 881), and Th. Ponticum. Pedigree analysis showed that the Xinong 979, Shaan 229,Shaan 213, and Xinong 881 were all derived from Xiaoyan 6. Xiaoyan 6 was a derivative from Th. ponticum. Six of106 specific molecular markers of Th. ponticum chromosome 7 E showed specific bands of Th. ponticum in Xinong 979 and Xiaoyan 6. Markers of Xwmc653 and Xwmc809 were located on 7 ES 75.10-77.46 cM; and Xcfd31 and Xgwm350 were located on 7 ES 86.16-87.32 cM; and Xmag1932 and Xdauk144 were located on 7 EL 147.71-149.51 cM. Theresults showed that Xinong 979 was a derivative from Xiaoyan 6. The study provides a reference for further researchand utilization of Xinong 979.
Xinong 979 is a famous facultative cultivar with improved quality, high yield potential, and multi-resistanceto various diseases, and has been widely extended in the southern Huanghuai Wheat Zone in China. Chromosome 7 Eof Thinopyrum ponticum carries Fusarium head blight(FHB) resistance gene(Fhb7), leaf rust resistance gene(Lr19)and stem rust resistance gene(Sr25). In this study, the pedigree and 7 E chromosome molecular marker of Th. ponticumwere used to analyze the genetic relationship among Xinong 979 and its 5 donor parents(Xiaoyan 6, Zaoyou 504,Shaan 229, Shaan 213, and Xinong 881), and Th. Ponticum. Pedigree analysis showed that the Xinong 979, Shaan 229,Shaan 213, and Xinong 881 were all derived from Xiaoyan 6. Xiaoyan 6 was a derivative from Th. ponticum. Six of106 specific molecular markers of Th. ponticum chromosome 7 E showed specific bands of Th. ponticum in Xinong 979 and Xiaoyan 6. Markers of Xwmc653 and Xwmc809 were located on 7 ES 75.10-77.46 cM; and Xcfd31 and Xgwm350 were located on 7 ES 86.16-87.32 cM; and Xmag1932 and Xdauk144 were located on 7 EL 147.71-149.51 cM. Theresults showed that Xinong 979 was a derivative from Xiaoyan 6. The study provides a reference for further researchand utilization of Xinong 979.
引文
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[19]Zhang X L,Shen X R,Hao Y Y,et al. A genetic map of Lophopyrumponticum chromosome 7E,harboring resistance genes to Fusariumhead blight and leaf rust. Theoretical and Applied Genetics,2011,122(2):263-270.
[20]Mullan D J,Platteter A,Teakle N L,et al. EST-derived SSR markersfrom defined regions of the wheat genome to identify Lophopyrumelongatum specific loci. Genome,2005,48(5):811-822.
[21]Zhang L Y,Bernard M,Leroy P,et al. High transferability of breadwheat EST-derived SSRs to other cereals. Theoretical and AppliedGenetics,2005,111(4):677-687.
[22]Yu J K,Dake T M,Singh S,et al. Development and mapping ofEST-derived simple sequence repeat markers for hexaploid wheat.Genome,2004,47(5):805-818.
[23]Chen H M,Li L Z,Wei X Y,et al. Development,chromosomelocation and genetic mapping of EST-SSR markers in wheat.Chinese Science Bulletin,2005,50(20):2328-2336.
[24]Xue S L,Zhang Z Z,Lin F,et al. A high-density intervarietal mapof the wheat genome enriched with markers derived from expressedsequence tags. Theoretical and Applied Genetics,2008,117(2):181-189.
[25]Ayala-Navarrete L,Bariana H S,Singh R P,et al. Trigenomicchromosomes by recombination of Thinopyrum intermedium andTh.ponticum translocations in wheat. Theoretical and AppliedGenetics,2007,116(1):63-75.
[26]裴阿卫,王怡,庞红喜,等.强筋型优质小麦新品种陕253的选育研究(Ⅰ).中国农学通报,2004,20(4):101-103.
[27]张荣琦,陈春环,赵晓农,等.利用远缘杂交技术选育小麦新品种之研究.中国农学通报,2006,22(6):186-188.
[28]宁锟,王怡.陕229小麦新品种选育及其特性研究.西北农业学报,1996,5(2):15-18.
[29]Cuthbert P A,Somers D J,Thomas J,et al. Fine mapping Fhb1,a major gene controlling fusarium head blight resistance in breadwheat(Triticum aestivum L.). Theretical and Applied Genetics,2006,112(8):1465-1472.
[30]刘新伦,王超,牛丽华,等.普通小麦-十倍体长穗偃麦草衍生新品种抗赤霉病基因的分子鉴别.中国农业科学,2017,50(20):3908-3917.
[2]Dvo?ák J,Chen K C. Phylogenetic relationships between chromosomesof wheat and chromosome 2E of Elytrigia elongata. Canadian Journalof Genetics and Cytalogy,1984,26(2):128-132.
[3]Liu Z,Li D Y,Zhang X Y. Genetic relationships among five basicgenomes St,E,A,B and D in Triticeae revealed by genomic southernand in situ hybridization. Journal of Integrative Plant Biology,2007,49(7):1080-1086.
[4]Ayala-Navarrete L,Mechanicos A A,Gibson J M,et al. The Pontinseries of recombinant alien translocations in bread wheat:singletranslocations integrating combinations of Bdv2,Lr19 and Sr25disease-resistance genes from Thinopyrum intermedium and Th.ponticum. Theoretical and Applied Genetics,2013,126(10):2467-2475.
[5]Zheng Q,Lv Z L,Niu Z X,et al. Molecular cytogenetic characterizationand stem rust resistance of five wheat-Thinopyrum ponticum partialamphiploids. Journal of Genetics and Genomics,2014,41(11):591-599.
[6]Shen X R,Kong L R,Ohm H. Fusarium head blight resistance inhexaploid wheat(Triticum aestivum)-Lophopyrum genetic linesand tagging of the alien chromatin by PCR markers. Theoretical andApplied Genetics,2004,108(5):808-813.
[7]Shen X,Ohm H. Molecular mapping of Thinopyrum-derived Fusariumhead blight resistance in common wheat. Molecular Breeding,2007,20(2):131-140.
[8]Guo J,Zhang X L,Hou Y L,et al. High-density mapping of the majorFHB resistance gene Fhb7 derived from Thinopyrum ponticum and itspyramiding with Fhb1 by marker-assisted selection. Theoretical andApplied Genetics,2015,128(11):2301-2316.
[9]Ceoloni C,Forte P,Gennaro A,et al. Recent developments in durumwheat chromosome engineering. Cytogenetic and Genome Research,2005,109(1/2/3):328-344.
[10]Li H,Wang X. Thinopyrum ponticum and Th.intermedium:thepromising source of resistance to fungal and viral diseases of wheat.Journal of Genetics and Genomics,2009,36(9):557-565.
[11]Zheng Q,Luo Q L,Niu Z X,et al. Variation in chromosomeconstitution of the Xiaoyan series partial amphiploids and itsrelationship to stripe rust and stem rust resistance. Journal ofGenetics and Genomics,2015,42(11):657-660.
[12]李振声.小麦远缘杂交新品种——小偃6号.山西农业科学,1986(5):30.
[13]Tao F,Wang J J,Guo Z F,et al. Transcriptomic analysis reveal themolecular mechanisms of wheat higher-temperature seedling-plantresistance to Puccinia striiformis f. sp. Tritici. Frontiers in PlantScience,2018,9:240.
[14]李琼,王长友,刘新伦,等.小偃6号及其衍生品种(系)遗传多样性的SSR分析.麦类作物学报,2008,28(6):950-955.
[15]张玲丽,孙道杰,冯毅,等.西农979抗赤霉病基因Fhb1的分子鉴定及其亲缘关系分析.麦类作物学报,2014,34(9):1199-1204.
[16]Yang Y,Zhao X L,Xia L Q,et al. Development and validation ofa Viviparous-1 STS marker for pre-harvest sprouting tolerance inChinese wheats. Theoretical and Applied Genetics,2007,115(7):971-980.
[17]孙道杰,张玲丽,冯毅,等.西农系列小麦骨干新品种赤霉病抗源浅析.麦类作物学报,2016,36(6):822-823.
[18]Guo J,He F,Cai J J,et al. Molecular and cytological comparisonsof chromosomes 7el(1),7el(2),7E(e),and 7E(i)derived fromThinopyrum. Cytogenetic and Genome Research,2015,145(1):68-74.
[19]Zhang X L,Shen X R,Hao Y Y,et al. A genetic map of Lophopyrumponticum chromosome 7E,harboring resistance genes to Fusariumhead blight and leaf rust. Theoretical and Applied Genetics,2011,122(2):263-270.
[20]Mullan D J,Platteter A,Teakle N L,et al. EST-derived SSR markersfrom defined regions of the wheat genome to identify Lophopyrumelongatum specific loci. Genome,2005,48(5):811-822.
[21]Zhang L Y,Bernard M,Leroy P,et al. High transferability of breadwheat EST-derived SSRs to other cereals. Theoretical and AppliedGenetics,2005,111(4):677-687.
[22]Yu J K,Dake T M,Singh S,et al. Development and mapping ofEST-derived simple sequence repeat markers for hexaploid wheat.Genome,2004,47(5):805-818.
[23]Chen H M,Li L Z,Wei X Y,et al. Development,chromosomelocation and genetic mapping of EST-SSR markers in wheat.Chinese Science Bulletin,2005,50(20):2328-2336.
[24]Xue S L,Zhang Z Z,Lin F,et al. A high-density intervarietal mapof the wheat genome enriched with markers derived from expressedsequence tags. Theoretical and Applied Genetics,2008,117(2):181-189.
[25]Ayala-Navarrete L,Bariana H S,Singh R P,et al. Trigenomicchromosomes by recombination of Thinopyrum intermedium andTh.ponticum translocations in wheat. Theoretical and AppliedGenetics,2007,116(1):63-75.
[26]裴阿卫,王怡,庞红喜,等.强筋型优质小麦新品种陕253的选育研究(Ⅰ).中国农学通报,2004,20(4):101-103.
[27]张荣琦,陈春环,赵晓农,等.利用远缘杂交技术选育小麦新品种之研究.中国农学通报,2006,22(6):186-188.
[28]宁锟,王怡.陕229小麦新品种选育及其特性研究.西北农业学报,1996,5(2):15-18.
[29]Cuthbert P A,Somers D J,Thomas J,et al. Fine mapping Fhb1,a major gene controlling fusarium head blight resistance in breadwheat(Triticum aestivum L.). Theretical and Applied Genetics,2006,112(8):1465-1472.
[30]刘新伦,王超,牛丽华,等.普通小麦-十倍体长穗偃麦草衍生新品种抗赤霉病基因的分子鉴别.中国农业科学,2017,50(20):3908-3917.
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