2018年我国部分地区牛病毒性腹泻病毒感染情况调查
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摘要
为了解我国目前牛病毒性腹泻病毒(BVDV)的感染情况,在内蒙古通辽、林西和吉林九台、安徽阜阳、山东聊城、江苏徐州等地区分别采集105份、89份、55份、97份、66份、39份犊牛血清,共计451份样品。通过BVDV血清中和试验对犊牛血清进行抗体检测,同时通过PT-PCR进行抗原的检测。结果显示不同地区采集的样品,BVDV血清抗体阳性率从25.6%～91.1%不等;RT-PCR检测的BVDV抗原阳性率为0～5.2%。本次调查BVDV通过对抗体与抗原的检测,以期为我国BVD的防治提供依据。
In order to understand the current situation of bovine viral diarrhea virus (BVDV) infection in China,clinical samples were collected from Tongliao, Linxi, Jilin Jiutai, Fuyang, Liaocheng, Jiangsu and other areas in Inner Mongolia. There were 105, 89, 55, 97, 66 and 39 samples were collected, totaling of 451 samples, mainly through BVDV serum neutralization method and BVDV Elisa antibody detection kit were used to detect the antibodies of calves collected from different areas. At the same time, the antigens were detected by PT-PCR. BVDV antibody detection results showed that the positive rates of samples collected from different areas ranged from 25.6% to 91.1%. The positive rate of bovine viral diarrhea virus antigen was 0~ 5.2% by RT-PCR. The detection of bovine viral diarrhea virus antibody and antigen provided the basis for the prevention and treatment of bovine viral diarrhea in China.
In order to understand the current situation of bovine viral diarrhea virus (BVDV) infection in China,clinical samples were collected from Tongliao, Linxi, Jilin Jiutai, Fuyang, Liaocheng, Jiangsu and other areas in Inner Mongolia. There were 105, 89, 55, 97, 66 and 39 samples were collected, totaling of 451 samples, mainly through BVDV serum neutralization method and BVDV Elisa antibody detection kit were used to detect the antibodies of calves collected from different areas. At the same time, the antigens were detected by PT-PCR. BVDV antibody detection results showed that the positive rates of samples collected from different areas ranged from 25.6% to 91.1%. The positive rate of bovine viral diarrhea virus antigen was 0~ 5.2% by RT-PCR. The detection of bovine viral diarrhea virus antibody and antigen provided the basis for the prevention and treatment of bovine viral diarrhea in China.
引文
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[11]童钦,吕晓妍,王炜,等.内蒙古牛病毒性腹泻病毒血清学调查[J].中国农学通报,2013,29(5):26-29.
[12]王建领,付彤,刘杰,等.牛病毒性腹泻分子及血清流行病学研究进展[J].河南农业科学,2013,41(3):7-11.
[2]李佑民,刘振润,武银莲.关于牛病毒性腹泻-黏膜病病毒(长春184)的分离与鉴定[J].中国人民解放军兽医大学学报,1993,3(2):113-121.
[3]张淑琴,郭利,冷雪,等.牛病毒性腹泻病毒BVDV-JL株的分离与鉴定[J].中国预防兽医学报,2011,33(3):181-184.
[4]殷震,刘景华.动物病毒学(第2版)[M].北京:科学出版社,1997.
[5]兰宇,孟相秋,赵建增.牛病毒性腹泻的免疫预防[J].中国动物保健,2016,2(18):29-31.
[6]王建领,付彤,刘杰,等.牛病毒性腹泻分子及血清流行病学研究进展[J].河南农业科学,2012,41(3):7-11.
[7]李海涛,苗利光,朱言柱,等.牛传染性鼻气管炎病毒JZ06-8株犊牛感染模型的建立[J].动物医学进展,2015,36(6):115-118.
[8]李佑民,刘振润,武银莲.关于牛病毒性腹泻-黏膜病病毒(长春184)的分离与鉴定[J].中国人民解放军兽医大学学报,1993,3(2):113-121.
[9]郑志刚,刘佩兰,郑增忍,等.关于牛病毒性腹泻/粘膜病血清中和抗体的调查报告[J].动物检疫,1991,05:40-42.
[10]王新平,刘红,宣华,等.绵羊感染牛病毒性腹泻-粘膜病病毒的调查研究[J].兽医大学学报,1993,03:8-9.
[11]童钦,吕晓妍,王炜,等.内蒙古牛病毒性腹泻病毒血清学调查[J].中国农学通报,2013,29(5):26-29.
[12]王建领,付彤,刘杰,等.牛病毒性腹泻分子及血清流行病学研究进展[J].河南农业科学,2013,41(3):7-11.
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