盐胁迫下益生菌对玉米的促生效应研究
|
摘要
为提高盐渍化农田作物的耐盐性,从生长于高盐土壤的玉米根际中分离促生潜力的菌株,在NaCl胁迫下,测定其对玉米发芽率、发芽势、促生长和电导率的影响。结果表明,枯草芽孢杆菌NY-06、巨大芽孢杆菌YJ-1和解淀粉芽孢杆菌YJ-3有很好益生效果。在1.5%NaCl和2.0%NaCl胁迫下,3个菌株发酵液处理后的玉米株高分别增高16.3%、9.5%、16.7%和18.9%、8.3%、19.9%,地上部鲜重分别增加28.8%、21.5%、34.0%和29.3%、22.8%、36.3%,根系鲜重分别增加32.7%、15.5%、18.3%和51.9%、58.6%、56.8%,玉米叶片相对电导率分别降低11.1%、9.2%、8.0%和6.4%、9.5%、14.2%。
To improve the salt tolerance of salinized farmland crops, the strains with promoting growth potential were isolated from the rhizosphere of maize grown in high salt soil. Under the stress of NaCl, we determined their ef-fects on maize germination rate, germination potential, growth rate and blade electrical conductivity. The results showed that Bacillus subtilis NY-06, Bacillus megaterium YJ-1 and Bacillus amyloliquefaciens YJ-3 had beneficial effects on maize growth. When maize plants were drenched with the strains fermentation broth,, the three strains in-creased 16.3%, 9.5%, 16.7% of the plant height of maize, 28.8%, 21.5%, 34.0% of the fresh weight of above-ground parts, 32.7%, 15.5%, 18.2% of root fresh weight, and decreased 11.1%, 9.2%, 8.0% of blade relative conduc-tivity, respectively, under the stress of 1.5% NaCl. And under 2.0% NaCl stress, the three strains fermentation broth had the same influencing trends on maize plant as that under 1.5% NaCl stress, which increased plant height by 18.9%, 8.3%, 19.9%, increased above-ground fresh weight by 29.2%, 22.8%, 36.3%, increased root fresh weight by 51.9%, 58.6% and 56.8%, and decreased the relative conductivity of maize leaves by 6.4%, 9.5%, 14.2%, respec-tively.
To improve the salt tolerance of salinized farmland crops, the strains with promoting growth potential were isolated from the rhizosphere of maize grown in high salt soil. Under the stress of NaCl, we determined their ef-fects on maize germination rate, germination potential, growth rate and blade electrical conductivity. The results showed that Bacillus subtilis NY-06, Bacillus megaterium YJ-1 and Bacillus amyloliquefaciens YJ-3 had beneficial effects on maize growth. When maize plants were drenched with the strains fermentation broth,, the three strains in-creased 16.3%, 9.5%, 16.7% of the plant height of maize, 28.8%, 21.5%, 34.0% of the fresh weight of above-ground parts, 32.7%, 15.5%, 18.2% of root fresh weight, and decreased 11.1%, 9.2%, 8.0% of blade relative conduc-tivity, respectively, under the stress of 1.5% NaCl. And under 2.0% NaCl stress, the three strains fermentation broth had the same influencing trends on maize plant as that under 1.5% NaCl stress, which increased plant height by 18.9%, 8.3%, 19.9%, increased above-ground fresh weight by 29.2%, 22.8%, 36.3%, increased root fresh weight by 51.9%, 58.6% and 56.8%, and decreased the relative conductivity of maize leaves by 6.4%, 9.5%, 14.2%, respec-tively.
引文
[1]张建锋.盐碱地的生态修复研究[J].水土保持研究,2008,15(4):74-78.Zhang J F.Discussion on ecological rehabilitation of salt-affected soils[J].Research of Soil and Water Conservation,2008,15(4):74-78.(in Chinese)
[2]孔涛,张德胜,徐慧,等.盐碱地及其改良过程中土壤微生物生态特征研究进展[J].土壤,2014,46(4):581-588.Kong T,Zhang D S,Xu H,et al.Microbial ecological characteristics of alkaline-saline lands and its amelioration process:A review[J].Soils,2014,46(4):581-588.(in Chinese)
[3]闫洪雪,刘露,李丽,等.PGPR的研究进展及其在农业上的应用[J].黑龙江农业科学,2016(6):148-151.Yan H X,Liu L,Li L,et al.Research progress on agriculture of plant growth promoting rhizobacteria[J].Heilongjiang Agricultural Sciences,2016(6):148-151.(in Chinese)
[4]陈晓斌,张炳欣.植物根围促生细菌(PGPR)作用机制的研究进展[J].微生物学杂志,2000,20(1):38-41,44.Chen X B,Zhang B X.Research progress on the mechanism of plant root enrichment promoting bacteria[J].Journal of Microbiolo-gy,2000,20(1):38-41,44.(in Chinese)
[5]王继雯,谢宝恩,周伏忠,等.高产吲哚乙酸及高泌氨巴西固氮螺菌的筛选与鉴定[J].生物技术,2009,19(6):14-17.Wang J W,Xie B E,Zhou F Z,et al.Isolation and identification of azospirillum brasilense excreting high concentration of IAA and am-monium[J].Biotechnology,2009,19(6):14-17.(in Chinese)
[6]刘军,辛树权,赵骥民.氯化钠胁迫下植物根际促生菌对白三叶草生长的影响[J].北方园艺,2014(3):65-69.Liu J,Xin S Q,Zhao J M.Effect of pant growth promoting bacteria on growth of butch clover stressed with NaCl[J].Northern Horticul-ture,2014(3):65-69.(in Chinese)
[7]Zhou N,Zhao S,Tian C Y.Effect of halotolerant rhizobacteria isolat-ed from halophytes on the growth of sugar beet(Beta vulgaris L.)un-der salt stress[J].Fems Microbiology Letters,2017,364(11):1-8.
[8]马晓敏,张红娟,顾沐宇,等.促生菌的分离鉴定及其对柳枝稷种子在盐胁迫下的萌发促进作用[J].家畜生态学报,2015,36(6):46-52.Ma X M,Zhang H J,Gu M Y,et al.Isolation and identification of plant growth-promoting rhizobacteria(pgpr)and its promoting effect on switchgrass seed under NaCl salt stress[J].Acta Ecologiae Ani-malis Domastici,2015,36(6):46-52.(in Chinese)
[9]秦媛.盐碱地植物共生微生物资源及功能初步研究[D].北京:中国林业科学研究院,2017.
[10]冯中红,王玉琴,杨成德.番茄细菌性叶斑病菌的拮抗菌筛选、鉴定及其拮抗性能评价[J].草业学报,2015,24(8):166-173.Feng Z H,Wang Y Q,Yang C D,et al.Screening,identification and assessment of endophytic bacteria antagonistic totomato bacterial spot[J].Acta Prataculturae Sinica,2015,24(8):166-173.(in Chi-nese)
[11]王玉凤.玉米苗期对NaCl胁迫的响应与耐盐性调控机理的研究[D].沈阳:沈阳农业大学,2008.
[12]李继兵.五株芽孢杆菌新种的分离纯化及其鉴定研究[D].上海:上海海洋大学,2015.
[13]Wang F,Yuan Q H,Shi L,et al.A large-scale field study of trans-gene flow from cultivated rice(Oryza sativa)to common wild rice(O.rufipogon)and barnyard grass(Echinochloa crusgalli)[J].Plant Bio-technology Journal,2006,4(6):667-676.
[14]赵骥民,辛树权,苏艳芳.NaCl溶液胁迫下促生菌对玉米幼苗的影响[J].吉林师范大学学报(自然科学版),2008,29(4):4-7.Zhao J M,Xin S Q,Su Y F.Effect of NaCl Liquor Stress PGPR on Growth of Corn Seedlings[J].Journal of Jilin Normal University(Nat-ural Science Edition),2008,29(4):4-7.(in Chinese)
[15]李华山,雷鹏,许宗奇,等.耐盐促生菌Agrobacterium sp.DF-2增强黄瓜幼苗耐盐性的研究[J].江苏农业学报,2017,33(3):654-661.Li H S,Lei P,Xu Z Q,et al.Halotolerance in cucumber seedlings enhanced by plant growth-promoting rhizobacterium Agrobacteri-um sp.DF-2[J].Jiangsu J.of Agr.Sci.,2017,33(3):654-661.(in Chinese)
[16]Bharti N,Pandey S S,Barnawal D,et al.Plant growth promoting rhi-zobacteria Dietzia natronolimnaea modulates the expression of stress responsive genes providing protection of wheat from salinity stress[J].Scientific Reports,2016,6:34768.
[17]Singh R P,Jha P,Jha P N.The plant-growth-promoting bacterium Klebsiella sp.SBP-8 confers induced systemic tolerance in wheat(Triticum aestivum)under salt stress[J].Journal of Plant Physiolo-gy,2015,184:57-67.
[18]Karlidag H,Yildirim E,Turan M,et al.Plant growth-promoting rhi-zobacteria mitigate deleterious effects of salt stress on strawberry plants(Fragaria 3ananassa)[J].Hortscience A Publication of the American Society for Horticultural Science,2013,5(48):563-567.
[19]付艳,高树仁,杨克军,等.盐胁迫对玉米耐盐系与盐敏感系苗期几个生理生化指标的影响[J].植物生理学报,2011,47(5):459-462.Fu Y,Gao S R,Yang K J,et al.Effects of salt stress on several physiological and biochemical indicators in seedling of salt-toler-ant line and salt-sensitive line of maize(Zea mays L.)[J].Plant Physiology Journal,2011,47(5):459-462.(in Chinese)
[20]莫文萍,李春,郑元元,等.盐胁迫下解盐促生菌对棉花种子发芽过程的影响[J].农业工程学报,2006,22(8):260-263.Mo W P,Li C,Zheng Y Y,et al.Effect of relieving-salt-stress and growth-promoting bacteria on germination of cot ton seed under salt stress[J].Transactions of the CSAE,2006,22(8):260-263.(in Chinese)
[21]Sarig S,Okon Y,Blum A.Promotion of leaf area development and yield in Sorghum bicolor inoculated with Azospirillum brasilense[J].Symbiosis,1990,9:235-245.
[22]江绪文,陈嘉斌,李贺勤,等.芽孢杆菌DY-3提高烟草幼苗的耐盐性[J].植物生理学报,2016(6):941-947.Jing X W,Chen J B,Li H Q,et al.Bacillus aquimaris strain DY-3improves salt-tolerance of tobacco seedlings[J].Plant Physiology Journal,2016(6):941-947.(in Chinese)
[23]牛舒琪,何傲蕾,丁新宇,等.枯草芽孢杆菌GB03与保水剂互作对小花碱茅生长和耐盐性的影响[J].植物生理学报,2016,52(3):285-292.Niu S Q,He A L,Ding X Y,et al.Effects of Bacillus subtilis GB03and water retaining agent on growth and salt tolerance in Puccinel-lia tenuiflora[J].Plant Physiology Journal,2016,52(3):285-292.(in Chinese)
[24]柳洁,肖斌,王丽霞,等.丛枝菌根真菌对茶树耐盐性的影响[J].西北农林科技大学学报(自然科学版),2014,42(3):220-225.Liu J,Xiao B,Wang L X,et al.Influence of AMF on salt tolerance of tea[J].Journal of Northwest A&F University(Nat.Sci.Ed.),2014,42(3):220-225.(in Chinese)
[2]孔涛,张德胜,徐慧,等.盐碱地及其改良过程中土壤微生物生态特征研究进展[J].土壤,2014,46(4):581-588.Kong T,Zhang D S,Xu H,et al.Microbial ecological characteristics of alkaline-saline lands and its amelioration process:A review[J].Soils,2014,46(4):581-588.(in Chinese)
[3]闫洪雪,刘露,李丽,等.PGPR的研究进展及其在农业上的应用[J].黑龙江农业科学,2016(6):148-151.Yan H X,Liu L,Li L,et al.Research progress on agriculture of plant growth promoting rhizobacteria[J].Heilongjiang Agricultural Sciences,2016(6):148-151.(in Chinese)
[4]陈晓斌,张炳欣.植物根围促生细菌(PGPR)作用机制的研究进展[J].微生物学杂志,2000,20(1):38-41,44.Chen X B,Zhang B X.Research progress on the mechanism of plant root enrichment promoting bacteria[J].Journal of Microbiolo-gy,2000,20(1):38-41,44.(in Chinese)
[5]王继雯,谢宝恩,周伏忠,等.高产吲哚乙酸及高泌氨巴西固氮螺菌的筛选与鉴定[J].生物技术,2009,19(6):14-17.Wang J W,Xie B E,Zhou F Z,et al.Isolation and identification of azospirillum brasilense excreting high concentration of IAA and am-monium[J].Biotechnology,2009,19(6):14-17.(in Chinese)
[6]刘军,辛树权,赵骥民.氯化钠胁迫下植物根际促生菌对白三叶草生长的影响[J].北方园艺,2014(3):65-69.Liu J,Xin S Q,Zhao J M.Effect of pant growth promoting bacteria on growth of butch clover stressed with NaCl[J].Northern Horticul-ture,2014(3):65-69.(in Chinese)
[7]Zhou N,Zhao S,Tian C Y.Effect of halotolerant rhizobacteria isolat-ed from halophytes on the growth of sugar beet(Beta vulgaris L.)un-der salt stress[J].Fems Microbiology Letters,2017,364(11):1-8.
[8]马晓敏,张红娟,顾沐宇,等.促生菌的分离鉴定及其对柳枝稷种子在盐胁迫下的萌发促进作用[J].家畜生态学报,2015,36(6):46-52.Ma X M,Zhang H J,Gu M Y,et al.Isolation and identification of plant growth-promoting rhizobacteria(pgpr)and its promoting effect on switchgrass seed under NaCl salt stress[J].Acta Ecologiae Ani-malis Domastici,2015,36(6):46-52.(in Chinese)
[9]秦媛.盐碱地植物共生微生物资源及功能初步研究[D].北京:中国林业科学研究院,2017.
[10]冯中红,王玉琴,杨成德.番茄细菌性叶斑病菌的拮抗菌筛选、鉴定及其拮抗性能评价[J].草业学报,2015,24(8):166-173.Feng Z H,Wang Y Q,Yang C D,et al.Screening,identification and assessment of endophytic bacteria antagonistic totomato bacterial spot[J].Acta Prataculturae Sinica,2015,24(8):166-173.(in Chi-nese)
[11]王玉凤.玉米苗期对NaCl胁迫的响应与耐盐性调控机理的研究[D].沈阳:沈阳农业大学,2008.
[12]李继兵.五株芽孢杆菌新种的分离纯化及其鉴定研究[D].上海:上海海洋大学,2015.
[13]Wang F,Yuan Q H,Shi L,et al.A large-scale field study of trans-gene flow from cultivated rice(Oryza sativa)to common wild rice(O.rufipogon)and barnyard grass(Echinochloa crusgalli)[J].Plant Bio-technology Journal,2006,4(6):667-676.
[14]赵骥民,辛树权,苏艳芳.NaCl溶液胁迫下促生菌对玉米幼苗的影响[J].吉林师范大学学报(自然科学版),2008,29(4):4-7.Zhao J M,Xin S Q,Su Y F.Effect of NaCl Liquor Stress PGPR on Growth of Corn Seedlings[J].Journal of Jilin Normal University(Nat-ural Science Edition),2008,29(4):4-7.(in Chinese)
[15]李华山,雷鹏,许宗奇,等.耐盐促生菌Agrobacterium sp.DF-2增强黄瓜幼苗耐盐性的研究[J].江苏农业学报,2017,33(3):654-661.Li H S,Lei P,Xu Z Q,et al.Halotolerance in cucumber seedlings enhanced by plant growth-promoting rhizobacterium Agrobacteri-um sp.DF-2[J].Jiangsu J.of Agr.Sci.,2017,33(3):654-661.(in Chinese)
[16]Bharti N,Pandey S S,Barnawal D,et al.Plant growth promoting rhi-zobacteria Dietzia natronolimnaea modulates the expression of stress responsive genes providing protection of wheat from salinity stress[J].Scientific Reports,2016,6:34768.
[17]Singh R P,Jha P,Jha P N.The plant-growth-promoting bacterium Klebsiella sp.SBP-8 confers induced systemic tolerance in wheat(Triticum aestivum)under salt stress[J].Journal of Plant Physiolo-gy,2015,184:57-67.
[18]Karlidag H,Yildirim E,Turan M,et al.Plant growth-promoting rhi-zobacteria mitigate deleterious effects of salt stress on strawberry plants(Fragaria 3ananassa)[J].Hortscience A Publication of the American Society for Horticultural Science,2013,5(48):563-567.
[19]付艳,高树仁,杨克军,等.盐胁迫对玉米耐盐系与盐敏感系苗期几个生理生化指标的影响[J].植物生理学报,2011,47(5):459-462.Fu Y,Gao S R,Yang K J,et al.Effects of salt stress on several physiological and biochemical indicators in seedling of salt-toler-ant line and salt-sensitive line of maize(Zea mays L.)[J].Plant Physiology Journal,2011,47(5):459-462.(in Chinese)
[20]莫文萍,李春,郑元元,等.盐胁迫下解盐促生菌对棉花种子发芽过程的影响[J].农业工程学报,2006,22(8):260-263.Mo W P,Li C,Zheng Y Y,et al.Effect of relieving-salt-stress and growth-promoting bacteria on germination of cot ton seed under salt stress[J].Transactions of the CSAE,2006,22(8):260-263.(in Chinese)
[21]Sarig S,Okon Y,Blum A.Promotion of leaf area development and yield in Sorghum bicolor inoculated with Azospirillum brasilense[J].Symbiosis,1990,9:235-245.
[22]江绪文,陈嘉斌,李贺勤,等.芽孢杆菌DY-3提高烟草幼苗的耐盐性[J].植物生理学报,2016(6):941-947.Jing X W,Chen J B,Li H Q,et al.Bacillus aquimaris strain DY-3improves salt-tolerance of tobacco seedlings[J].Plant Physiology Journal,2016(6):941-947.(in Chinese)
[23]牛舒琪,何傲蕾,丁新宇,等.枯草芽孢杆菌GB03与保水剂互作对小花碱茅生长和耐盐性的影响[J].植物生理学报,2016,52(3):285-292.Niu S Q,He A L,Ding X Y,et al.Effects of Bacillus subtilis GB03and water retaining agent on growth and salt tolerance in Puccinel-lia tenuiflora[J].Plant Physiology Journal,2016,52(3):285-292.(in Chinese)
[24]柳洁,肖斌,王丽霞,等.丛枝菌根真菌对茶树耐盐性的影响[J].西北农林科技大学学报(自然科学版),2014,42(3):220-225.Liu J,Xiao B,Wang L X,et al.Influence of AMF on salt tolerance of tea[J].Journal of Northwest A&F University(Nat.Sci.Ed.),2014,42(3):220-225.(in Chinese)
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |