互叶白千层优株筛选及丛生芽诱导培养
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摘要
本研究以3种互叶白千层枝叶为试验材料,采用同时蒸馏法提取精油,通过气相色谱-质谱(GC-MS)联用分析鉴定其化学成分。在此基础上,采用正交试验对优株诱导培养基的激素种类和浓度进行优化。结果发现,互叶白千层品种2精油中松油醇-4含量为42. 9%,1,8-桉树油素含量为11. 24%,较佳的丛生芽诱导培养基为MS+0. 05 mg/L NAA+0. 15 mg/L 6-BA+0. 05%活性炭+0. 07 mmol/L硝酸镧,p H值5. 8,该条件下的诱导率是96. 4%。结果表明,互叶白千层品种2是高精油优良株系,精油成分符合国际标准(ISO4730—1996),上述丛生芽诱导培养基为最佳诱导培养基。
引文
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[3]Souza M E,Lopes L Q,Bonez P C,et al. Melaleuca alternifolia nanoparticles against Candida species biofilms[J]. Microbial Pathogenesis,2017,104(3):125-132.
[4]Comin V M,Lopes L Q,Quatrin P M,et al. Influence of Melaleuca alternifolia oil nanoparticles on aspects of Pseudomonas aeruginosa biofilm[J]. Microbial Pathogenesis,2016,93(4):120-125.
[5]Baldissera M D,Souza C F,Júnior G B,et al. Melaleuca alternifolia essential oil enhances the non-specific immune system and prevents oxidative damage in Rhamdia quelen experimentally infected by Aeromonas hydrophila:Effects on cholinergic and purinergic systems in liver tissue[J]. Fish&Shellfish Immunology,2017,61(2):1-8.
[6]Souza C D F,Baldissera M D,Vaucher R D A,et al. In vivo bactericidal effect of Melaleuca alternifolia essential oil against Aeromonas hydrophila:silver catfish(Rhamdia quelen)as an experimental model[J]. Microbial Pathogenesis,2016,98(9):82-87.
[7]吴頔,谢吉蓉,宋琴,等.茶树油作为天然抗菌剂的研究进展[J].中国药学杂志,2013,48(21):1803-1807.
[8]Pereira T S,de Sant'anna J R,Silva E L,et al. In vitro genotoxicity of Melaleuca alternifolia essential oil in human lymphocytes[J]. Journal of Ethnopharmacology,2014,151(2):852-857.
[9]Garozzo A,Timpanaro R,Stivala A,et al. Activity of Melaleuca alternifolia(tea tree)oil on influenza virus a/PR/8:study on the mechanism of action[J]. Antiviral Research,2011,89(1):83-88.
[10]Wong Y F,Davies N W,Chin S T,et al. Enantiomeric distribution of selected terpenes for authenticity assessment of Australian Melaleuca alternifolia oil[J]. Industrial Crops and Products,2015,67(5):475-483.
[11]Baldissera M D,da Silva A S,Oliveira C B,et al. Effect of tea tree oil(Melaleuca alternifolia)on the longevity and immune response of rats infected by Trypanosoma evansi[J]. Research in Veterinary Science,2014,96(3):501-506.
[12]Santamaria J,Petermann K D,Vedovello S A,et al. Antimicrobial effect of Melaleuca alternifolia dental gel in orthodontic patients[J].American Journal of Orthodontics and Dentofacial Orthopedics,2014,145(2):198-202.
[13]吴丽君,陈达,高楠,等.高含油互叶白千层高效栽培配套技术研究[J].福建农业学报,2017,32(7):734-738.
[14]Shepherd M,Wood R,Raymond C,et al. Ecotype variation in early growth,coppicing,and shoot architecture of tea tree(Melaleuca alternifolia)[J]. Industrial Crops and Products,2015,76(12):844-856.
[15]Russell M F,Southwell I A. Monoterpenoid accumulation in 1,8-cineole,terpinolene and terpinen-4-ol chemotypes of Melaleuca alternifolia seedlings[J]. Phytochemistry,2003,62(5):683-689.
[16]Anon. Essential oils-oil of Melaeuca,terpinen-4-ol type(tea tree oil). ISO-4730[R]. Geneva:International Organisation for Standardisation,1996.
[17]黄戈,翁晓晨,张帆,等.互叶白千层的种植和加工研究进展[J].广东化工,2017,44(4):71-73.
[18]覃子海,肖玉菲,唐复呈,等.互叶白千层扦插繁殖技术[J].广西林业科学,2017,46(1):111-114.