谷子SSR标记与光周期敏感性的关联分析
|
摘要
对光周期敏感是导致谷子生态适应性狭窄、生产上缺少跨区大品种的重要原因,鉴定谷子光周期敏感性相关联QTL位点是进一步揭示敏感形成遗传机制的基础。首先在长日照地区(河南省洛阳市)和短日照地区(海南省乐东县)对45份谷子品种进行抽穗期表型性状调查,并根据两地的抽穗期差异计算出光周期敏感值,最后进行SSR标记与光周期敏感性的关联分析。结果表明,40个SSR标记在45个谷子品种中共检测到150个等位基因,平均每个引物有3. 75个等位基因,其中引物b200和b124的等位基因数目最多,均为6个。利用SSR标记对45份谷子材料进行群体遗传结构分析得到最佳K值为3,即将其划分为3个亚群:其中第1亚群有5个品种,第2亚群有16个品种,第3亚群有21个品种,而剩下的3个品种则不能划分到任何一组,成为混合群。连锁不平衡分析表明,40个SSR标记间不存在明显的连锁不平衡结构。SSR标记与光周期敏感性的关联分析检测到b200(SSR8)和b127(SSR35) 2个位点与光周期敏感性关联(P <0. 05)。
Sensitivity to photoperiod is the main reason for narrow ecological adaptability and lacking cross-regional cultivars of foxtail millet. Identification of QTLs associated with millet photoperiod sensitivity is the foundation for further exploring genetic mechanisms of photoperiod sensitivity. 45 millet cultivars were surveyed for heading date in long-day region( Luoyang City,Henan Province) and short-day region( Ledong County,Hainan Province). The photoperiod sensitivity values of 45 millet cultivars were calculated according to the difference between the heading date in the two regions. Then,40 pairs of SSR markers were used to determine the genotypes of these millet cultivars. Finally association analysis between SSR markers and photoperiod sensitivity was performed. The results showed that a total of 150 alleles were detected in 45 millet cultivars,giving an average allele number of3. 75 per locus. Of the 40 SSR markers,b200 and b124 amplified the most number of alleles( 6 alleles per locus).Based on the SSR markers amplification data,the population genetic structure of 45 millet cultivars was analyzed,and the optimal K value was 3,so the 45 millet cultivars were divided into three subgroups. The first subgroup included 5 cultivars,the second subgroup included 16 cultivars and the third subgroup included 21 cultivars. The remaining three cultivars could not be grouped into any of the three subgroups,consisted of a mixed group. The linkage disequilibrium analysis showed that no significant linkage disequilibrium structures were found among 40 SSR markers. Finally association analysis between SSR markers and photoperiod sensitivity were performed,two loci,b200( SSR8) and b127( SSR35) were detected to associate with photoperiod sensitivity( P < 0. 05).
Sensitivity to photoperiod is the main reason for narrow ecological adaptability and lacking cross-regional cultivars of foxtail millet. Identification of QTLs associated with millet photoperiod sensitivity is the foundation for further exploring genetic mechanisms of photoperiod sensitivity. 45 millet cultivars were surveyed for heading date in long-day region( Luoyang City,Henan Province) and short-day region( Ledong County,Hainan Province). The photoperiod sensitivity values of 45 millet cultivars were calculated according to the difference between the heading date in the two regions. Then,40 pairs of SSR markers were used to determine the genotypes of these millet cultivars. Finally association analysis between SSR markers and photoperiod sensitivity was performed. The results showed that a total of 150 alleles were detected in 45 millet cultivars,giving an average allele number of3. 75 per locus. Of the 40 SSR markers,b200 and b124 amplified the most number of alleles( 6 alleles per locus).Based on the SSR markers amplification data,the population genetic structure of 45 millet cultivars was analyzed,and the optimal K value was 3,so the 45 millet cultivars were divided into three subgroups. The first subgroup included 5 cultivars,the second subgroup included 16 cultivars and the third subgroup included 21 cultivars. The remaining three cultivars could not be grouped into any of the three subgroups,consisted of a mixed group. The linkage disequilibrium analysis showed that no significant linkage disequilibrium structures were found among 40 SSR markers. Finally association analysis between SSR markers and photoperiod sensitivity were performed,two loci,b200( SSR8) and b127( SSR35) were detected to associate with photoperiod sensitivity( P < 0. 05).
引文
[1]邓杰,戴林建,杨苏,周田.光温条件对烤烟生长发育和品质形成的影响综述[J].作物研究,2015,9(6):678-681. doi:10. 3969/j. issn. 1001-5280.2015. 06. 26.Deng J,Dai L J,Yang S,Zhou T. Research progress oneffect of light and temperature on the growth and quality offlue-cured tobacco[J]. Crop Research,2015,9(6):678-681. doi:10. 3969/j. issn. 1001-5280. 2015. 06. 26.
[2] Garner W W,Allard H A. Effect of relative length of dayand night and other factors of the environment on growthand reproduction in plants[J]. J Agric Res,1920,18:553-606.
[3]唐志明,李晓方,庄国富,金晓春,卢东柏.水稻株高差异与光温生态敏感性的关系初探[J].广东农业科学,2007(4):7-10. doi:10. 3969/j. issn. 1004-874X. 2007. 04. 002.Tang Z M,Li X F,Zhuang G F,Jin X C,Lu D B.Searching for the correlative factors of photosensitivenessand temperature sensibility in rice[J]. Guangdong Agri-cultural Sciences,2007(4):7-10. doi:10. 3969/j.issn. 1004-874X. 2007. 04. 002.
[4]王翠玲,程芳芳,孙朝晖,库丽霞,陈晓,陈彦惠.玉米光周期敏感性的遗传特性及相关基因的研究进展[J].玉米科学,2008,16(1):11-14,19.Wang C L,Cheng F F,Sun C H,Ku L X,Chen X,Chen Y H. Advances in genetic research and relatedgenes of photoperiod sensitivity in maize[J]. Journal ofMaize Science,2008,16(1):11-14,19.
[5]杨玉杰,李海云.光周期对植物生长发育的影响研究进展[J].安徽农业科学,2010,38(13):6693-6694,6740. doi:10. 3969/j. issn. 0517-6611. 2010. 13.026.Yang Y J,Li H Y. Research progress on the effect ofphotoperiod on plant growth and development[J]. Journalof Anhui Agricultural Science,2010,38(13):6693-6694,6740. doi:10. 3969/j. issn. 0517-6611. 2010. 13.026.
[6]沈韫赜,郭双生.受控环境下光周期对小麦生长发育、产量及营养品质的影响[J].麦类作物学报,2015,35(1):64-70. doi:10. 7606/j. issn. 1009-1041. 2015. 01. 10.Shen Y Z,Guo S S. Effects of photoperiod on growth,de-velopment,yield and nutritional quality of wheat in con-trolled system[J]. Journal of Triticeae Crops,2015,35(1):64-70. doi:10. 7606/j. issn. 1009-1041. 2015. 01.10.
[7]李雪,温昕,邵铁梅,孔卫娜,刘培,焦展,安胜军.芝麻遗传工程的研究进展[J].中国农学通报,2012,28(6):153-158. doi:10. 3969/j. issn. 1000-6850.2012. 06. 029.Li X,Wen X,Shao T M,Kong W N,Liu P,Jiao Z,AnS J. Progress on genetic engineering of sesame[J]. Chi-nese Agricultural Science Bulletin,2012,28(6):153-158. doi:10. 3969/j. issn. 1000-6850. 2012. 06. 029.
[8]赵治海,崔文生,杜贵,杨少青.谷子光(温)敏不育系821选育及其不育性与光温关系的研究[J].中国农业科学,1996,29(5):23-31.Zhao Z H,Cui W S,Du G,Yang S Q. The selection ofmillet photo(thermo)sensitive sterile line 821 and a studyon the relation of sterility to illumination and temperature[J]. Scientia Agricultura Sinica,1996,29(5):23-31.
[9]贾小平,袁玺垒,李剑峰,张博,张小梅,郭秀璞,陈春燕.不同光温条件谷子资源主要农艺性状的综合评价[J].中国农业科学,2018,51(13):578-1752.doi:10. 3864/j. issn. 0578-1752. 2018. 13. 001.Jia X P,Yuan X L,Li J F,Zhang B,Zhang X M,GuoX P,Chen C Y. Comprehensive evaluation of main agro-nomic traits of millet resources under different light andtemperature conditions[J]. Scientia Agricultura Sinica,2018,51(13):578-1752. doi:10. 3864/j. issn. 0578-1752. 2018. 13. 001.
[10]贾小平,李剑峰,全建章,王永芳,董志平,张博,袁玺垒.不同光周期条件下谷子农艺性状的光周期敏感性评价[J].植物遗传资源学报,2018,19(5):919-924. doi:10. 13430/j. cnki. jpgr. 20180207005.Jia X P,Li J F,Quan J Z,Wang Y F,Dong Z P,Zhang B,Yuan X L. Evaluation of photoperiod sensitiv-ity of agronomic traits of foxtail millet varieties(Setariaitalica)under different photoperiod conditions[J]. Jour-nal of Plant Genetic Resources,2018,19(5):919-924. doi:10. 13430/j. cnki. jpgr. 20180207005.
[11]谢丽莉.谷子光周期敏感相关性状的QTL定位与分析[D].郑州:河南农业大学,2012.Xie L L. QTL mapping and analysis of photoperiod sen-sitivity of agronomic traits of foxtail millet varieties(Se-taria italica)[D]. Zhengzhou:Henan Agricultural Uni-versity,2012.
[12] Margarita Mauro-Herrera,Wang X W,Hugues Barbier,Thomas Brutnell,Katrien M Devos,Andrew N Doust.Genetic control and comparative genomic analysis offlowering time in setaria(poaceae)[J]. G3,2013,3(2):283-295. doi:10. 1534/g3. 112. 005207.
[13]贾小平,董普辉,张红晓,孔祥生.不同谷子品种(系)生长发育特性及抗倒性分析[J].河南农业科学,2015,44(8):27-31. doi:10. 15933/j. cnki.1004-3268. 2015. 08. 006.Jia X P,Dong P H,Zhang H X,Kong X S. Analysis ofgrowth and development characteristics and lodging re-sistance of different foxtail millet cultivars(strains)[J].Journal of Henan Agricultural Sciences,2015,44(8):27-31. doi:10. 15933/j. cnki. 1004-3268. 2015. 08.006.
[14]贾小平,袁玺垒,陆平,范丙友,黄华,戴凌峰.中国71个谷子种质资源的灰色关联度分析及综合评价[J].种子,2017,36(9):1001-4705. doi:10.16590/j. cnki. 1001-4705. 2017. 09. 063.Jia X P,Yuan X L,Lu P,Fan B Y,Huang H,Dai LF. Grey incidence analysis and comprehensive evalua-tion of 71 millet germplasm resources in China[J].Seed,2017,36(9):1001-4705. doi:10. 16590/j.cnki. 1001-4705. 2017. 09. 063.
[15]贾小平.谷子SSR标记的开发及遗传图谱的构建[D].北京:中国农业大学,2008.Jia X P. Development and genetic mapping of SSRmarkers in foxtail millet(Setaria italica)[D]. Beijing:China Agricultural University,2008.
[16] Evanno G,Regnaut S,Goudet J. Detecting the numberof clusters of individuals using the software STRUC-TURE:a simulation study[J]. Molecular Ecology,2005,14(8):2611-2620. doi:10. 1111/j. 1365-294X. 2005. 02553. x.
[17] Doust A N,Mauro-Herrera M,Hodgeand J G,JessicaS. The C4model grass Setaria is a short day plant withsecondary long day genetic regulation[J]. Frontiers inPlant Science,2017(8):1-10. doi:10. 3389/fpls.
[18] Jia G,Huang X,Zhi H,Zhao Y,Zhao Q,Li W,ChaiY,Yang L,Liu K,Lu H,Zhu C,Lu Y,Zhou C,FanD,Weng Q,Guo Y,Huang T,Zhang L,Lu T,FengQ,Hao H,Liu H,Lu P,Zhang N,Li Y,Guo E,Wang S,Wang S,Liu J,Zhang W,Chen G,Zhang B,Li W,Wang Y,Li H,Zhao B,Li J,Diao X,Han B.A haplotype map of genomic variations and genome-wideassociation studies of agronomic traits in foxtail millet(Setaria italica)[J]. Nature Genetics,2013,45(8):957-961. doi:10. 1038/ng. 2673.
[19] Gupta S,Kumari K,Muthamilarasan M,Parida S K,Prasad M. Population structure and association mappingof yield contributing agronomic traits in foxtail millet[J]. Plant Cell Reports,2014,33(6):881-893. doi:10. 1007/s00299-014-1564-0.
[20] Pajoro A,Severing E,Angenent G C,Immink R. His-tone H3 lysine 36 methylation affects temperature-in-duced alternative splicing and flowering in plants[J].Genome Biology,2017,18(102):1-12. doi:10. 1186/s13059-017-1235-x.
[21] Choi S C,Lee S,Kim S R,Lee Y S,Liu C Y,Cao X F,An G H. Trithorax group protein Oryza sativa Trithorax1controls flowering time in rice via interaction with Earlyheading date 3[J]. Plant Physiol,2014,164:1326-1337. doi:10. 1104/pp. 113. 228049.
[22]段桂芳.拟南芥F-box家族基因FOA2的功能初步研究[D].长沙:湖南大学,2014.Duan G F. Preliminary study on the function of F-boxfamily gene FOA2 in Arabidopsis thaliana[D]. Chang-sha:Hunan University,2014.
[2] Garner W W,Allard H A. Effect of relative length of dayand night and other factors of the environment on growthand reproduction in plants[J]. J Agric Res,1920,18:553-606.
[3]唐志明,李晓方,庄国富,金晓春,卢东柏.水稻株高差异与光温生态敏感性的关系初探[J].广东农业科学,2007(4):7-10. doi:10. 3969/j. issn. 1004-874X. 2007. 04. 002.Tang Z M,Li X F,Zhuang G F,Jin X C,Lu D B.Searching for the correlative factors of photosensitivenessand temperature sensibility in rice[J]. Guangdong Agri-cultural Sciences,2007(4):7-10. doi:10. 3969/j.issn. 1004-874X. 2007. 04. 002.
[4]王翠玲,程芳芳,孙朝晖,库丽霞,陈晓,陈彦惠.玉米光周期敏感性的遗传特性及相关基因的研究进展[J].玉米科学,2008,16(1):11-14,19.Wang C L,Cheng F F,Sun C H,Ku L X,Chen X,Chen Y H. Advances in genetic research and relatedgenes of photoperiod sensitivity in maize[J]. Journal ofMaize Science,2008,16(1):11-14,19.
[5]杨玉杰,李海云.光周期对植物生长发育的影响研究进展[J].安徽农业科学,2010,38(13):6693-6694,6740. doi:10. 3969/j. issn. 0517-6611. 2010. 13.026.Yang Y J,Li H Y. Research progress on the effect ofphotoperiod on plant growth and development[J]. Journalof Anhui Agricultural Science,2010,38(13):6693-6694,6740. doi:10. 3969/j. issn. 0517-6611. 2010. 13.026.
[6]沈韫赜,郭双生.受控环境下光周期对小麦生长发育、产量及营养品质的影响[J].麦类作物学报,2015,35(1):64-70. doi:10. 7606/j. issn. 1009-1041. 2015. 01. 10.Shen Y Z,Guo S S. Effects of photoperiod on growth,de-velopment,yield and nutritional quality of wheat in con-trolled system[J]. Journal of Triticeae Crops,2015,35(1):64-70. doi:10. 7606/j. issn. 1009-1041. 2015. 01.10.
[7]李雪,温昕,邵铁梅,孔卫娜,刘培,焦展,安胜军.芝麻遗传工程的研究进展[J].中国农学通报,2012,28(6):153-158. doi:10. 3969/j. issn. 1000-6850.2012. 06. 029.Li X,Wen X,Shao T M,Kong W N,Liu P,Jiao Z,AnS J. Progress on genetic engineering of sesame[J]. Chi-nese Agricultural Science Bulletin,2012,28(6):153-158. doi:10. 3969/j. issn. 1000-6850. 2012. 06. 029.
[8]赵治海,崔文生,杜贵,杨少青.谷子光(温)敏不育系821选育及其不育性与光温关系的研究[J].中国农业科学,1996,29(5):23-31.Zhao Z H,Cui W S,Du G,Yang S Q. The selection ofmillet photo(thermo)sensitive sterile line 821 and a studyon the relation of sterility to illumination and temperature[J]. Scientia Agricultura Sinica,1996,29(5):23-31.
[9]贾小平,袁玺垒,李剑峰,张博,张小梅,郭秀璞,陈春燕.不同光温条件谷子资源主要农艺性状的综合评价[J].中国农业科学,2018,51(13):578-1752.doi:10. 3864/j. issn. 0578-1752. 2018. 13. 001.Jia X P,Yuan X L,Li J F,Zhang B,Zhang X M,GuoX P,Chen C Y. Comprehensive evaluation of main agro-nomic traits of millet resources under different light andtemperature conditions[J]. Scientia Agricultura Sinica,2018,51(13):578-1752. doi:10. 3864/j. issn. 0578-1752. 2018. 13. 001.
[10]贾小平,李剑峰,全建章,王永芳,董志平,张博,袁玺垒.不同光周期条件下谷子农艺性状的光周期敏感性评价[J].植物遗传资源学报,2018,19(5):919-924. doi:10. 13430/j. cnki. jpgr. 20180207005.Jia X P,Li J F,Quan J Z,Wang Y F,Dong Z P,Zhang B,Yuan X L. Evaluation of photoperiod sensitiv-ity of agronomic traits of foxtail millet varieties(Setariaitalica)under different photoperiod conditions[J]. Jour-nal of Plant Genetic Resources,2018,19(5):919-924. doi:10. 13430/j. cnki. jpgr. 20180207005.
[11]谢丽莉.谷子光周期敏感相关性状的QTL定位与分析[D].郑州:河南农业大学,2012.Xie L L. QTL mapping and analysis of photoperiod sen-sitivity of agronomic traits of foxtail millet varieties(Se-taria italica)[D]. Zhengzhou:Henan Agricultural Uni-versity,2012.
[12] Margarita Mauro-Herrera,Wang X W,Hugues Barbier,Thomas Brutnell,Katrien M Devos,Andrew N Doust.Genetic control and comparative genomic analysis offlowering time in setaria(poaceae)[J]. G3,2013,3(2):283-295. doi:10. 1534/g3. 112. 005207.
[13]贾小平,董普辉,张红晓,孔祥生.不同谷子品种(系)生长发育特性及抗倒性分析[J].河南农业科学,2015,44(8):27-31. doi:10. 15933/j. cnki.1004-3268. 2015. 08. 006.Jia X P,Dong P H,Zhang H X,Kong X S. Analysis ofgrowth and development characteristics and lodging re-sistance of different foxtail millet cultivars(strains)[J].Journal of Henan Agricultural Sciences,2015,44(8):27-31. doi:10. 15933/j. cnki. 1004-3268. 2015. 08.006.
[14]贾小平,袁玺垒,陆平,范丙友,黄华,戴凌峰.中国71个谷子种质资源的灰色关联度分析及综合评价[J].种子,2017,36(9):1001-4705. doi:10.16590/j. cnki. 1001-4705. 2017. 09. 063.Jia X P,Yuan X L,Lu P,Fan B Y,Huang H,Dai LF. Grey incidence analysis and comprehensive evalua-tion of 71 millet germplasm resources in China[J].Seed,2017,36(9):1001-4705. doi:10. 16590/j.cnki. 1001-4705. 2017. 09. 063.
[15]贾小平.谷子SSR标记的开发及遗传图谱的构建[D].北京:中国农业大学,2008.Jia X P. Development and genetic mapping of SSRmarkers in foxtail millet(Setaria italica)[D]. Beijing:China Agricultural University,2008.
[16] Evanno G,Regnaut S,Goudet J. Detecting the numberof clusters of individuals using the software STRUC-TURE:a simulation study[J]. Molecular Ecology,2005,14(8):2611-2620. doi:10. 1111/j. 1365-294X. 2005. 02553. x.
[17] Doust A N,Mauro-Herrera M,Hodgeand J G,JessicaS. The C4model grass Setaria is a short day plant withsecondary long day genetic regulation[J]. Frontiers inPlant Science,2017(8):1-10. doi:10. 3389/fpls.
[18] Jia G,Huang X,Zhi H,Zhao Y,Zhao Q,Li W,ChaiY,Yang L,Liu K,Lu H,Zhu C,Lu Y,Zhou C,FanD,Weng Q,Guo Y,Huang T,Zhang L,Lu T,FengQ,Hao H,Liu H,Lu P,Zhang N,Li Y,Guo E,Wang S,Wang S,Liu J,Zhang W,Chen G,Zhang B,Li W,Wang Y,Li H,Zhao B,Li J,Diao X,Han B.A haplotype map of genomic variations and genome-wideassociation studies of agronomic traits in foxtail millet(Setaria italica)[J]. Nature Genetics,2013,45(8):957-961. doi:10. 1038/ng. 2673.
[19] Gupta S,Kumari K,Muthamilarasan M,Parida S K,Prasad M. Population structure and association mappingof yield contributing agronomic traits in foxtail millet[J]. Plant Cell Reports,2014,33(6):881-893. doi:10. 1007/s00299-014-1564-0.
[20] Pajoro A,Severing E,Angenent G C,Immink R. His-tone H3 lysine 36 methylation affects temperature-in-duced alternative splicing and flowering in plants[J].Genome Biology,2017,18(102):1-12. doi:10. 1186/s13059-017-1235-x.
[21] Choi S C,Lee S,Kim S R,Lee Y S,Liu C Y,Cao X F,An G H. Trithorax group protein Oryza sativa Trithorax1controls flowering time in rice via interaction with Earlyheading date 3[J]. Plant Physiol,2014,164:1326-1337. doi:10. 1104/pp. 113. 228049.
[22]段桂芳.拟南芥F-box家族基因FOA2的功能初步研究[D].长沙:湖南大学,2014.Duan G F. Preliminary study on the function of F-boxfamily gene FOA2 in Arabidopsis thaliana[D]. Chang-sha:Hunan University,2014.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |