前列腺癌患者血清miRNA-194和miRNA-206表达水平及临床意义
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摘要
目的探讨前列腺癌(PCa)患者血清miRNA-194和miRNA-206表达水平及临床意义。方法选取2008年5月～2014年12月我院收治的108例PCa患者为PCa组,以及同期就诊的60例良性前列腺增生(BPH)患者为BPH组、前列腺正常者60例为正常组,分析所有纳入研究者的临床及随访资料,记录年龄等一般临床资料和血清miR-NA-194和miRNA-206等指标水平及PCa患者肿瘤及预后情况,并比较不同PCa患者间上述资料的差异性。结果BPH组和PCa组患者血清miRNA-206水平较正常组低(P<0.05),而血清PSA水平和miRNA-194较正常组高(P<0.05);且PCa患者其血清miRN A-206水平较BHP患者更低,而血清PSA和miRNA-194水平则更高(P<0.05);Gl-eason评分大于7分、临床分期T3～T4期以及出现淋巴结转移和术后复发的PCa患者其血清miRNA-206水平均显著降低,而血清miRNA-194显著升高(P<0.05);死亡患者血清miRNA-206水平均显著低于生存者,而血清miRNA-194水平显著高于生存者(P<0.05);经Log-rank检验,Gleason评分大于7分、临床分期T3～T4期、出现淋巴结转移、术后复发以及血清miR NA-194水平升高和miRNA-206水平降低的PCa患者其生存时间均较短(P<0.05);经多因素Cox比例风险回归模型分析,淋巴结转移、术后复发以及血清miRNA-194升高和miRNA-206水平降低均为影响PCa患者预后的独立影响因素(P<0.05)。结论血清miRNA-194在PCa患者中显著上升,而血清miRNA-206水平显著下降,且血清miRNA-194的上升和miRNA-206的下降均为影响PCa患者预后的独立影响因素,因此临床上可将其作为评估PCa患者病情及预后的有效指标。
Objective To investigate the expression levels and clinical significance of serum miRNA-194 and miRNA-206 in patients with prostate cancer.Methods 108 PCa patients in our hospital from May 2008 to December 2014 and 60 patients with benign prostatic hyperplasia(BPH)and 60 patients with normal prostate in our hospital were selected as subjects.The clinical and follow-up data of all included investigators were retrospectively analyzed.The general clinical data including the age of the investigator and the serum miRNA-194 and miRNA-206 levels and the tumor and prognosis of PCa patients were recorded,and the differences of the above data among different PCa patients were compared.Results BPH and PCa patients had lower serum miRNA-206 levels than normal,while serum PSA and miRNA-194 levels were higher than those of the control group;and PCa patients had lower serum miRN A-206 levels than BHP patients and higher serum PSA and miRNA-194 levels(P<0.05).Gleason score is greater than 7 points,The serum levels of microRNA-206 in patients with T3-T4 clinical stage and PCA with lymph node metastasis and postoperative recurrence were significantly decreased.However,serum miRNA-194 and serum levels increased significantly(P<0.05).Serum miRNA-206 levels were significantly lower in dead patients than in survivors;serum miRNA-194 levels were significantly higher in dead patients than survivors(P<0.05).Log-rank test showed that,gleason score was greater than 7 points,clinical stage T3～T4 phase,lymph node metastasis,adjacent tissue organ invasion,postoperative recurrence,high serum miR NA-194 level and low miRNA-206 level had lower 3-year overall survival rate(P<0.05).According to the multivariate Cox proportional hazards regression model,lymph node metastasis,adjacent tissue organ invasion,postoperative recurrence,elevated serum miRNA-194 and decreased miRN A-206 levels were independent factors influencing the prognosis of PCa patients(P<0.05).Conclusion Serum miRNA-194 is significantly elevated in PCa patients,while serum miRNA-206 levels is significantly decreased,and the rise of serum miRNA-194 and the decrease of serum miRNA-206 are independent factors affecting the prognosis of PCa patients,so it can be clinically used as an effective indicator for assessing the condition and prognosis of patients with PCa.
Objective To investigate the expression levels and clinical significance of serum miRNA-194 and miRNA-206 in patients with prostate cancer.Methods 108 PCa patients in our hospital from May 2008 to December 2014 and 60 patients with benign prostatic hyperplasia(BPH)and 60 patients with normal prostate in our hospital were selected as subjects.The clinical and follow-up data of all included investigators were retrospectively analyzed.The general clinical data including the age of the investigator and the serum miRNA-194 and miRNA-206 levels and the tumor and prognosis of PCa patients were recorded,and the differences of the above data among different PCa patients were compared.Results BPH and PCa patients had lower serum miRNA-206 levels than normal,while serum PSA and miRNA-194 levels were higher than those of the control group;and PCa patients had lower serum miRN A-206 levels than BHP patients and higher serum PSA and miRNA-194 levels(P<0.05).Gleason score is greater than 7 points,The serum levels of microRNA-206 in patients with T3-T4 clinical stage and PCA with lymph node metastasis and postoperative recurrence were significantly decreased.However,serum miRNA-194 and serum levels increased significantly(P<0.05).Serum miRNA-206 levels were significantly lower in dead patients than in survivors;serum miRNA-194 levels were significantly higher in dead patients than survivors(P<0.05).Log-rank test showed that,gleason score was greater than 7 points,clinical stage T3～T4 phase,lymph node metastasis,adjacent tissue organ invasion,postoperative recurrence,high serum miR NA-194 level and low miRNA-206 level had lower 3-year overall survival rate(P<0.05).According to the multivariate Cox proportional hazards regression model,lymph node metastasis,adjacent tissue organ invasion,postoperative recurrence,elevated serum miRNA-194 and decreased miRN A-206 levels were independent factors influencing the prognosis of PCa patients(P<0.05).Conclusion Serum miRNA-194 is significantly elevated in PCa patients,while serum miRNA-206 levels is significantly decreased,and the rise of serum miRNA-194 and the decrease of serum miRNA-206 are independent factors affecting the prognosis of PCa patients,so it can be clinically used as an effective indicator for assessing the condition and prognosis of patients with PCa.
引文
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[9]赵书润,孟琳.前列腺癌患者血清睾酮水平、前列腺雄激素受体表达与精索静脉曲张相关研究[J].癌症进展,2016,14(7):661-663.
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[13]陈强,蒋敏,李丹.干扰和过表达自分泌运动因子受体对A172细胞侵袭力的影响[J].医学分子生物学杂志,2016,13(2):84-89.
[14]陈昊,何屹,候岩松,等.血清骨性标记物NTx和BALP与前列腺癌骨转移的关系及临床意义[J].临床肿瘤学杂志,2016,21(12):1111-1113.
[15]周颖,吕梦琴,魏睿,等.血清缓激肽与前列腺癌的相关性研究[J].实用肿瘤杂志,2017,32(3):212-215.
[16]彭庆,谭倩,董传江,等.miR-21通过Wnt信号通路对前列腺癌生物学行为的影响[J].解放军医药杂志,2017,28(12):684-686.
[17]赵军,谢家恩.老年前列腺癌患者血清PSA水平与代谢综合征相关指标的相关性分析[J].国际泌尿系统杂志,2017,37(6):628-630.
[18] Wilczynski M,Danielska J,Dzieniecka M,et al.Prognostic andClinical Significance of miRNA-205in Endometrioid EndometrialCancer[J].Plos One,2016,11(10):0164687.
[19]全超,刘红岩,高伟兴,等.前列腺动脉栓塞治疗高危前列腺癌合并尿潴留的疗效观察[J].西部医学,2017,29(2):203-206.
[20]张蒙,盛镔,马鹏德,等.核糖体蛋白L6在前列腺癌中的表达及临床意义[J].天津医药,2016,44(1):75-78.
[21]刘志成,苑荣亮,张成,等.人星状病毒非结构蛋白nsP1a/1相互作用的宿主细胞靶蛋白筛选[J].医学分子生物学杂志,2016,13(5):281-285.
[22]吉喆,姜玉良,王俊杰,等.质子放疗的临床应用研究进展[J].西部医学,2016,28(4):580-584.
[23]汪峰,强亚勇,张斌斌,等.腹腔镜前列腺癌根治术治疗前列腺癌的疗效及对患者血清激素水平的影响[J].现代生物医学进展,2017,17(24):4710-4713.
[24] Chen X,Zhang Q,Ma W,et al.The Abnormal Expression ofMicroRNA-542-3p in Hepatoc ellular Carcinoma and Its ClinicalSignificance.[J].Disease Markers,2018,2018(1):1-9.
[25]张钢,赵海军,邓军,等.前列腺癌中CEACAM6和ANG2的表达及其临床意义[J].医学临床研究,2016,33(11):634-636.
[2]潘印,甘泉,卢军,等.前列腺癌患者血清miR-129、miR-21检测及临床意义[J].中国卫生检验杂志,2016,25(19):2807-2809.
[3]尤泊森,柳清.前列腺癌患者血清和病理组织中miRNA-375的表达及意义[J].中国肿瘤临床与康复,2017,23(4):428-431.
[4]戴金华,廖于峰,冷江涌,等.前列腺癌患者血清miR-146a与miR-152的表达水平及临床意义[J].中国性科学,2016,25(4):7-10.
[5]郭建桥.PTEN与TGF-β在前列腺癌中的表达及其临床意义[J].解放军医药杂志,2016,28(11):61-65.
[6]姚春红,袁新初,刘冲,等.miRNA-15a在前列腺癌中的表达及诊断意义[J].中国免疫学杂志,2016,32(8):1219-1221.
[7]崔莹莹,袁学华,李柯,等.五苓胶囊对前列腺癌循环miRNA-141表达的影响[J].现代生物医学进展,2017,30(11):2140-2143.
[8]夏中友,伍季,张萌,等.前列腺癌组织中ING4与VEGF的表达及相关性研究[J].西部医学,2017,29(2):187-191.
[9]赵书润,孟琳.前列腺癌患者血清睾酮水平、前列腺雄激素受体表达与精索静脉曲张相关研究[J].癌症进展,2016,14(7):661-663.
[10] Bakkar A,Alshalalfa M,Petersen L F,et al.microRNA 338-3p exhibits tumor suppressor role and its down-regulation is as-sociated with adverse clinical outcome in prostate cancer pa-tients.[J].Molecular Biology Reports,2016,43(4):229-240.
[11]陈水兰,王姣莲.前列腺癌手术前后血清Chemerin联合EPCA-2检测及其临床意义[J].国际泌尿系统杂志,2016,36(5):702-705.
[12]虞力航,徐刚.前列腺癌患者血清前列腺特异抗原水平与Glea-son评分相关性分析[J].中华全科医学,2016,14(5):771-773.
[13]陈强,蒋敏,李丹.干扰和过表达自分泌运动因子受体对A172细胞侵袭力的影响[J].医学分子生物学杂志,2016,13(2):84-89.
[14]陈昊,何屹,候岩松,等.血清骨性标记物NTx和BALP与前列腺癌骨转移的关系及临床意义[J].临床肿瘤学杂志,2016,21(12):1111-1113.
[15]周颖,吕梦琴,魏睿,等.血清缓激肽与前列腺癌的相关性研究[J].实用肿瘤杂志,2017,32(3):212-215.
[16]彭庆,谭倩,董传江,等.miR-21通过Wnt信号通路对前列腺癌生物学行为的影响[J].解放军医药杂志,2017,28(12):684-686.
[17]赵军,谢家恩.老年前列腺癌患者血清PSA水平与代谢综合征相关指标的相关性分析[J].国际泌尿系统杂志,2017,37(6):628-630.
[18] Wilczynski M,Danielska J,Dzieniecka M,et al.Prognostic andClinical Significance of miRNA-205in Endometrioid EndometrialCancer[J].Plos One,2016,11(10):0164687.
[19]全超,刘红岩,高伟兴,等.前列腺动脉栓塞治疗高危前列腺癌合并尿潴留的疗效观察[J].西部医学,2017,29(2):203-206.
[20]张蒙,盛镔,马鹏德,等.核糖体蛋白L6在前列腺癌中的表达及临床意义[J].天津医药,2016,44(1):75-78.
[21]刘志成,苑荣亮,张成,等.人星状病毒非结构蛋白nsP1a/1相互作用的宿主细胞靶蛋白筛选[J].医学分子生物学杂志,2016,13(5):281-285.
[22]吉喆,姜玉良,王俊杰,等.质子放疗的临床应用研究进展[J].西部医学,2016,28(4):580-584.
[23]汪峰,强亚勇,张斌斌,等.腹腔镜前列腺癌根治术治疗前列腺癌的疗效及对患者血清激素水平的影响[J].现代生物医学进展,2017,17(24):4710-4713.
[24] Chen X,Zhang Q,Ma W,et al.The Abnormal Expression ofMicroRNA-542-3p in Hepatoc ellular Carcinoma and Its ClinicalSignificance.[J].Disease Markers,2018,2018(1):1-9.
[25]张钢,赵海军,邓军,等.前列腺癌中CEACAM6和ANG2的表达及其临床意义[J].医学临床研究,2016,33(11):634-636.
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