晋中地区鸡致病性大肠杆菌分离株的鉴定及其16S rDNA序列的同源性分析
|
摘要
为了鉴定晋中地区鸡致病性大肠杆菌,利用传统细菌鉴定方法及16S rDNA序列分析,对分离自晋中地区养殖场送检的30只病死鸡肝中的疑似大肠杆菌菌株进行鉴定及遗传进化分析。结果表明:分离出8株致病性不同的大肠杆菌;鉴定出O78、O2、O11共3种血清型;8株菌均为多药耐药菌,其中对5种及5种以上抗菌药物耐药的菌株达到总分离菌株的87.5%;将8株菌株的16S rDNA基因序列与GenBank中登录的大肠杆菌参考株的16S rDNA基因序列进行比对,同源率均达到99%。经MEGA6.0软件分析后,其位于不同的分支上。本研究结果为进一步研究致病性大肠杆菌的耐药性和对晋中地区大肠杆菌病的有效防治奠定了一定基础。
This study aims to identify pathogenic Escherichia coli in chicken.A total of 30 random samples of sick or dead chickens were collected from Jinzhong Prefecture,and suspected E.coli in the liver was isolated and cultured.The isolated strains were detected by the traditional bacterial identification method and the 16S rDNA sequences were analyzed.The results showed that eight isolated E.coli strains had different pathogenicity.Three different serotypes were identified as O78,O2 and O11.All 8 strains were multi-drug resistant bacteria,among which the 5 or more strain resistant antimicrobial agents reached 87.5% of the total isolated strains.The 16 S r DNA gene sequences of 8 strains were contrasted with those of the E.coli reference strains in Gen Bank,and the homology ratio reached 99%.When analyzed with start software(MEGA6.0),the 16S rDNA gene sequences of 8 strains were located on different branches.The results showed that more study need to be conducted on the drug resistance of pathogenic E.coli to find an effective prevention and treatment of colibacilosis in Jinzhong Prefecture.
This study aims to identify pathogenic Escherichia coli in chicken.A total of 30 random samples of sick or dead chickens were collected from Jinzhong Prefecture,and suspected E.coli in the liver was isolated and cultured.The isolated strains were detected by the traditional bacterial identification method and the 16S rDNA sequences were analyzed.The results showed that eight isolated E.coli strains had different pathogenicity.Three different serotypes were identified as O78,O2 and O11.All 8 strains were multi-drug resistant bacteria,among which the 5 or more strain resistant antimicrobial agents reached 87.5% of the total isolated strains.The 16 S r DNA gene sequences of 8 strains were contrasted with those of the E.coli reference strains in Gen Bank,and the homology ratio reached 99%.When analyzed with start software(MEGA6.0),the 16S rDNA gene sequences of 8 strains were located on different branches.The results showed that more study need to be conducted on the drug resistance of pathogenic E.coli to find an effective prevention and treatment of colibacilosis in Jinzhong Prefecture.
引文
[1]马兴树,范翠蝶,夏玉龙.禽致病性大肠杆菌研究进展[J].中国畜牧兽医,2013,40(2):169-174.MA Xing-shu,FAN Cui-die,XIA Yu-long.Research progress in avian pathogenic Escherichia coli[J].China Animal Husbandry and Veterinary Medicine,2013,40(2):169-174.(in Chinese)
[2]吴清民.兽医传染病学[M].北京:中国农业大学出版社,2002.WU Qing-min.Veterinary Infectious Diseases[M].Beijing:China Agricultural University Press,2002.(in Chinese)
[3]林竹红,朱美兰,朱凤明.鸡大肠杆菌病及其防治[J].中国畜牧兽医文摘,2012(12):185-186.LIN Zhu-hong,ZHU Mei-lan,ZHU Feng-ming.Chicken Escherichia coli and its prevention and treatment[J].Chinese Journal of Animal Husbandry and Veterinary Medicine,2012(12):185-186.(in Chinese)
[4]夏业才,潘松年.禽大肠杆菌病的研究:鸡致病性大肠杆菌的血清型鉴定[J].中国兽药杂志,1991,25(4):1-2.XIA Ye-cai,PAN Song-nian.Avian Escherichia coli disease study:Serotype identification of chicken pathogenic Escherichia coli[J].Chinese Journal of Veterinary Drug,1991,25(4):1-2.(in Chinese)
[5]宋立,宁宜宝,张秀英,等.我国不同地区家禽大肠杆菌血清型分布和耐药性比较研究[J].中国农业科学,2005,38(7):1466-1473.SONG Li,NING Yi-bao,ZHANG Xiu-ying,et al.Comparative research on serotype distribution and antimicro bial resistance of Escherichia coli isolates from poultry in different areas of China[J].Scientia Agricultura Sinica,2005,38(7):1466-1473.(in Chinese)
[6]郑蓓,常维山.2011年我国部分地区鸡源大肠杆菌耐药性分析[J].山东畜牧兽医,2012,33:80-82.ZHENG Bei,CHANG Wei-shan.Analysis of drug resistance of chicken Escherichia coli in parts of China in 2011[J].Shandong Animal Husbandry and Veterinary Medicine,2012,33:80-82.(in Chinese)
[7]胡桂学.兽医微生物学实验教程[M].北京:中国农业大学出版社,2006.HU Gui-xue.Experimental Course of Veterinary Microbiology[M].Beijing:China Agricultural University Press,2006.(in Chinese)
[8]刘建钗,沈洁,闫金坤,等.鸡大肠杆菌病形态学与分子生物学的诊断[J].中国兽医科学,2016,46(2):217-222.LIU Jian-chai,SHEN Jie,YAN Jin-kun,et al.Diagnosis of chicken colibacillosis by morphological and molecular biological method[J].Chinese Veterinary Science,2016,46(2):217-222.(in Chinese)
[9]马越,李景云,金少鸿.美国临床实验室标准委员会推荐药敏试验操作方法和判断标准(2005年修订版)[J].中华医学杂志,2005,85(17):1182-1184.MA Yue,LI Jing-yun,JIN Shao-hong.American Clinical Laboratory Standards Committee recommends drug sensitivity test methods and criteria(2005revision)[J].Chinese Journal of Medicine,2005,85(17):1182-1184.(in Chinese)
[10]高荣琨,王丽芳,李锐,等.太原地区鸡源性大肠杆菌的分离鉴定及耐药性分析[J].中国预防兽医学报,2013,35(8):631-634.GAO Rong-kun,WANG Li-fang,LI Rui,et al.Isolation and identification of antibiotic resistance Escherichia coli from chicken farms in Taiyuan areas[J].Chinese Journal of Preventive Veterinary Medicine,2013,35(8):631-634.(in Chinese)
[11]宁官保,牛艺儒,张鼎,等.鸡源大肠杆菌耐药性分析及中药对大肠杆菌耐药性消除作用的研究[J].畜牧兽医学报,2015,46(6):1018-1025.NING Guan-bao,NIU Yi-ru,ZHANG Ding,et al.Studies on the drug resistance of chicken isolated Escherichia coli and role of traditional Chinese medicines in the drug resistance elimination[J].Acta Veterinaria et Zootechnica Sinica,2015,46(6):1018-1025.(in Chinese)
[12]潘树桥,周晓利,冯喜辉,等.复方中药制剂的制备及对肉用仔鸡大肠杆菌病的防治效果[J].中国兽医科学,2000,30(2):37-38.PAN Shu-qiao,ZHOU Xiao-li,FENG Xi-hui,et al.Preparation of compound traditional Chinese medicine preparation and its control effect on Escherichia coli in broilers[J].Chinese Veterinary Science,2000,30(2):37-38.(in Chinese)
[13]金福源,朱国良,姚小兵,等.鸡源大肠杆菌的分离鉴定及耐药性分析[J].中国畜禽种业,2017,13(7):150-152.JIN Fu-yuan,ZHU Guo-liang,YAO Xiao-bing,et al.Isolation,identification and drug resistance anal ysis of Escherichia coli from chicken[J].China Livestock and Poultry Industry,2017,13(7):150-152.(in Chinese)
[14]苏胜兵,马红霞,徐凤宇.噬菌体在细菌性疾病诊断和治疗中的应用[J].中国兽医科学,2011,41(5):546-550.SU Sheng-bing,MA Hong-xia,XU Feng-yu.Review of application of bacteriophage in the diagnosis and therapy for bacteria infection[J].Chinese Veterinary Science,2011,41(5):546-550.(in Chinese)
[15]罗婷婷,王睿,顾川佳,等.噬菌体治疗细菌性感染及其限制因素[J].微生物与感染,2017,12(2):120-125.LUO Ting-ting,WANG Rui,GU Chuan-jia,et al.Phage therapy and its limiting factors[J].Microorganisms and Infections,2017,12(2):120-125.(in Chinese)
[16]李延龙.胶州市鸡大肠杆菌病的病原分离鉴定及治疗研究[D].泰安:山东农业大学,2008.LI Yan-long.Isolation,identification and treatment of chicken colibacillosis in Jiaozhou City[D].Tai’an:Shandong Agricultural University,2008.(in Chinese)
[17]齐凤云.滨州地区规模化鸡场大肠杆菌分离鉴定和诊疗措施[D].泰安:山东农业大学,2012.QI Feng-yun.Separation,identification and diagnosis and treatment of Escherichia coli in largescale chicken farms in Binzhou area[D].Tai’an:Shandong Agricultural University,2012.(in Chinese)
[18]么乃全,李斯齐,邸海洋,等.火鸡多杀性巴氏杆菌的分离鉴定及致病性研究[J].中国兽医科学,2016,46(2):242-246.YAO Nai-quan,LI Si-qi,DI Hai-yang,et al.Isolation and identification of Pasteurella multocida from turkey fowl cholera and study on its pathogenicity[J].Chinese Veterinary Science,2016,46(2):242-246.(in Chinese)
[2]吴清民.兽医传染病学[M].北京:中国农业大学出版社,2002.WU Qing-min.Veterinary Infectious Diseases[M].Beijing:China Agricultural University Press,2002.(in Chinese)
[3]林竹红,朱美兰,朱凤明.鸡大肠杆菌病及其防治[J].中国畜牧兽医文摘,2012(12):185-186.LIN Zhu-hong,ZHU Mei-lan,ZHU Feng-ming.Chicken Escherichia coli and its prevention and treatment[J].Chinese Journal of Animal Husbandry and Veterinary Medicine,2012(12):185-186.(in Chinese)
[4]夏业才,潘松年.禽大肠杆菌病的研究:鸡致病性大肠杆菌的血清型鉴定[J].中国兽药杂志,1991,25(4):1-2.XIA Ye-cai,PAN Song-nian.Avian Escherichia coli disease study:Serotype identification of chicken pathogenic Escherichia coli[J].Chinese Journal of Veterinary Drug,1991,25(4):1-2.(in Chinese)
[5]宋立,宁宜宝,张秀英,等.我国不同地区家禽大肠杆菌血清型分布和耐药性比较研究[J].中国农业科学,2005,38(7):1466-1473.SONG Li,NING Yi-bao,ZHANG Xiu-ying,et al.Comparative research on serotype distribution and antimicro bial resistance of Escherichia coli isolates from poultry in different areas of China[J].Scientia Agricultura Sinica,2005,38(7):1466-1473.(in Chinese)
[6]郑蓓,常维山.2011年我国部分地区鸡源大肠杆菌耐药性分析[J].山东畜牧兽医,2012,33:80-82.ZHENG Bei,CHANG Wei-shan.Analysis of drug resistance of chicken Escherichia coli in parts of China in 2011[J].Shandong Animal Husbandry and Veterinary Medicine,2012,33:80-82.(in Chinese)
[7]胡桂学.兽医微生物学实验教程[M].北京:中国农业大学出版社,2006.HU Gui-xue.Experimental Course of Veterinary Microbiology[M].Beijing:China Agricultural University Press,2006.(in Chinese)
[8]刘建钗,沈洁,闫金坤,等.鸡大肠杆菌病形态学与分子生物学的诊断[J].中国兽医科学,2016,46(2):217-222.LIU Jian-chai,SHEN Jie,YAN Jin-kun,et al.Diagnosis of chicken colibacillosis by morphological and molecular biological method[J].Chinese Veterinary Science,2016,46(2):217-222.(in Chinese)
[9]马越,李景云,金少鸿.美国临床实验室标准委员会推荐药敏试验操作方法和判断标准(2005年修订版)[J].中华医学杂志,2005,85(17):1182-1184.MA Yue,LI Jing-yun,JIN Shao-hong.American Clinical Laboratory Standards Committee recommends drug sensitivity test methods and criteria(2005revision)[J].Chinese Journal of Medicine,2005,85(17):1182-1184.(in Chinese)
[10]高荣琨,王丽芳,李锐,等.太原地区鸡源性大肠杆菌的分离鉴定及耐药性分析[J].中国预防兽医学报,2013,35(8):631-634.GAO Rong-kun,WANG Li-fang,LI Rui,et al.Isolation and identification of antibiotic resistance Escherichia coli from chicken farms in Taiyuan areas[J].Chinese Journal of Preventive Veterinary Medicine,2013,35(8):631-634.(in Chinese)
[11]宁官保,牛艺儒,张鼎,等.鸡源大肠杆菌耐药性分析及中药对大肠杆菌耐药性消除作用的研究[J].畜牧兽医学报,2015,46(6):1018-1025.NING Guan-bao,NIU Yi-ru,ZHANG Ding,et al.Studies on the drug resistance of chicken isolated Escherichia coli and role of traditional Chinese medicines in the drug resistance elimination[J].Acta Veterinaria et Zootechnica Sinica,2015,46(6):1018-1025.(in Chinese)
[12]潘树桥,周晓利,冯喜辉,等.复方中药制剂的制备及对肉用仔鸡大肠杆菌病的防治效果[J].中国兽医科学,2000,30(2):37-38.PAN Shu-qiao,ZHOU Xiao-li,FENG Xi-hui,et al.Preparation of compound traditional Chinese medicine preparation and its control effect on Escherichia coli in broilers[J].Chinese Veterinary Science,2000,30(2):37-38.(in Chinese)
[13]金福源,朱国良,姚小兵,等.鸡源大肠杆菌的分离鉴定及耐药性分析[J].中国畜禽种业,2017,13(7):150-152.JIN Fu-yuan,ZHU Guo-liang,YAO Xiao-bing,et al.Isolation,identification and drug resistance anal ysis of Escherichia coli from chicken[J].China Livestock and Poultry Industry,2017,13(7):150-152.(in Chinese)
[14]苏胜兵,马红霞,徐凤宇.噬菌体在细菌性疾病诊断和治疗中的应用[J].中国兽医科学,2011,41(5):546-550.SU Sheng-bing,MA Hong-xia,XU Feng-yu.Review of application of bacteriophage in the diagnosis and therapy for bacteria infection[J].Chinese Veterinary Science,2011,41(5):546-550.(in Chinese)
[15]罗婷婷,王睿,顾川佳,等.噬菌体治疗细菌性感染及其限制因素[J].微生物与感染,2017,12(2):120-125.LUO Ting-ting,WANG Rui,GU Chuan-jia,et al.Phage therapy and its limiting factors[J].Microorganisms and Infections,2017,12(2):120-125.(in Chinese)
[16]李延龙.胶州市鸡大肠杆菌病的病原分离鉴定及治疗研究[D].泰安:山东农业大学,2008.LI Yan-long.Isolation,identification and treatment of chicken colibacillosis in Jiaozhou City[D].Tai’an:Shandong Agricultural University,2008.(in Chinese)
[17]齐凤云.滨州地区规模化鸡场大肠杆菌分离鉴定和诊疗措施[D].泰安:山东农业大学,2012.QI Feng-yun.Separation,identification and diagnosis and treatment of Escherichia coli in largescale chicken farms in Binzhou area[D].Tai’an:Shandong Agricultural University,2012.(in Chinese)
[18]么乃全,李斯齐,邸海洋,等.火鸡多杀性巴氏杆菌的分离鉴定及致病性研究[J].中国兽医科学,2016,46(2):242-246.YAO Nai-quan,LI Si-qi,DI Hai-yang,et al.Isolation and identification of Pasteurella multocida from turkey fowl cholera and study on its pathogenicity[J].Chinese Veterinary Science,2016,46(2):242-246.(in Chinese)
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |