玉米顶腐病病原细菌成团泛菌C3菌株对不同环境适应性的研究
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摘要
【目的】探究玉米顶腐病病原细菌——成团泛菌(Pantoea agglomerans) C3菌株对不同环境的适应性。【方法】通过稀释涂板法测定成团泛菌在不同温度、水质和寄主植物中的存活动态。【结果】C3菌株对紫外线辐射较为敏感,照射40 s后致死率达到100%;相较于37℃,4℃和20℃有利于C3菌株生存,灭菌自来水、水沟水和自来水中的菌体数量高于臭水沟水和池塘水;另外,C3菌株可在多种植物叶片上定殖,定殖密度表现为玉米>烟草>棉花>三叶草>稗草。【结论】成团泛菌具有很强的环境适应性,能够在不同的环境中进行生存和繁殖。
[Purpose]To investigate the environmental adaptation of Pantoea agglomrans C3 causing maize top rot.[Method]Its survival dynamic was detected in different temperatures, surface water of different quality and host plants with dilution plate method.[Result] C3 was sensitive to ultraviolet radiation(UVC), and all of its cells could be killed after 40 seconds radiation. Compared with 37 ℃, 4 ℃ and 20 ℃ facilitated this plant pathogen's survival. The number of C3 in sterilized tap water, ditch water and tap water were more than those in drainage water and pond water. In addition,C3 could colonize in various plant leaves, where the density order from high to low was maize, tobacco, cotton, clover and barnyard grass.[Conclusion]P. agglomerans has strong environmental adaptability and can survive and reproduce in different environments.
[Purpose]To investigate the environmental adaptation of Pantoea agglomrans C3 causing maize top rot.[Method]Its survival dynamic was detected in different temperatures, surface water of different quality and host plants with dilution plate method.[Result] C3 was sensitive to ultraviolet radiation(UVC), and all of its cells could be killed after 40 seconds radiation. Compared with 37 ℃, 4 ℃ and 20 ℃ facilitated this plant pathogen's survival. The number of C3 in sterilized tap water, ditch water and tap water were more than those in drainage water and pond water. In addition,C3 could colonize in various plant leaves, where the density order from high to low was maize, tobacco, cotton, clover and barnyard grass.[Conclusion]P. agglomerans has strong environmental adaptability and can survive and reproduce in different environments.
引文
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[10]贾鲁娜,潘立晶,陈铭德.细菌DNA紫外线辐射损伤和DNA修复系统的实验探究活动[J].生物学教学,2009,34(1):56.DOI:10.3969/j.issn.1004-7549.2009.01.031.
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[12]康萍芝,张丽荣,白小军,等.土壤环境因素对黄瓜枯萎病菌存活和生长繁殖的影响[J].宁夏农林科技,2017,58(10):32.DOI:10.3969/j.issn.1002-204X.2017.10.016.
[13]谷祖敏,王芳,纪明山,等.拮抗细菌B9对灰霉病菌的影响及在番茄叶表的定殖[J].云南农业大学学报,2006,21(6):842.DOI:10.3969/j.issn.1004-390X.2006.06.030.
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[16]BARASH I,MANULIS-SASSON S.Recent evolution of bacterial pathogens:the gall-forming Pantoea agglomerans case[J].Annual Review of Phytopathology,2009,47:133.DOI:10.1146/annurev-phyto-080508-081803.
[17]MORALES-VALENZUELA G,SILVA-ROJAS H V,OCHOA-MARTINEZ D.First report of Pantoea agglomerans causing leaf blight and vascular wilt in maize and sorghum in Mexico[J].Journal of Plant Diseases and Protection,2017,91(10):1365.DOI:10.1094/PDIS-91-10-1365A.
[18]曹慧英.玉米新病害-细菌干茎腐病的研究[D].北京:中国农业科学院,2010.
[19]杨君洋,卢洪洲.成团泛菌感染及其诊治的研究近况[J].上海医药,2016,37(3):48.
[20]CANAMAS T P,VINAS I,USALL J,et al.Control of postharvest diseases on citrus fruit by preharvest application of the biocontrol agent Pantoea agglomerans CPA-2:Part I.Study of different formulation strategies to improve survival of cells in unfavourable environmental conditions[J].Postharvest Biology and Technology,2008,49(1):86.DOI:10.1016/j.postharvbio.2007.12.006.
[21]王洪媛.抗UV-B辐射菌Pantoea agglomerans KFS-9的选育及其抗辐射机制的研究[D].青岛:中国海洋大学,2006.
[22]REZZONICO F,SMITS T H M,MONTESIONS E,et al.Genotypic comparison of Pantoea agglomerans plant and clinical strains[J].BMC microbiology,2009,9(1):204.DOI:10.1186/1471-2180-9-204.
[23]SELVAKUMAR G,KUNDU S,JOSHI P,et al.Characterization of a cold-tolerant plant growth-promoting bacterium Pantoea dispersa 1A isolated from a sub-alpine soil in the North Western Indian Himalayas[J].World Journal of Microbiology and Biotechnology,2008,24(7):955.DOI:10.1007/s11274-007-9558-5.
[24]WENNEKER M,VERDEL M S W,GROENEVELD RM W,et al.Ralstonia(Pseudomonas)solanacearum race3(biovar 2)in surface water and natural weed hosts:first report on stinging nettle(Urtica dioica)[J].European Journal of Plant Pathology,1999,105(3):307.DOI:10.1111/j.1439-0434.1999.tb03854.x.
[25]PRADHANANG P M,ELPHINSTONE J G,FOX R TV.Identification of crop and weed hosts of Ralstonia solanacearum biovar 2 in the hills of Nepal[J].Plant Pathology,2000,49(4):403.DOI:10.1046/j.1365-3059.2000.00480.x.
[26]COUTINHO T A,VENTER S N.Pantoea ananatis:an unconventional plant pathogen[J].Molecular Plant Pathology,2009,10(3):325.DOI:10.1111/j.1364-3703.2009.00542.x.
[2]林丽.云南省玉米细菌性顶腐病病原菌的分离与鉴定[D].昆明:云南农业大学,2013.
[3]NISSAN G,GERSHOVITS M,MOROZOV M,et al.Revealing the inventory of typeⅢeffectors in Pantoea agglomerans gall-forming pathovars using draft genome sequences and a machine-learning approach[J].Molecular Plant Pathology,2018,19(2):381.DOI:10.1111/mpp.12528.
[4]FENG Y,SHEN D,SONG W.Rice endophyte Pantoea agglomerans YS19 promotes host plant growth and affects allocations of host photosynthates[J].Journal of Applied Microbiology,2006,100(5):938.DOI:10.1111/j.1365-2672.2006.02843.x.
[5]XIE J,SHU P,STROBEL G,et al.Pantoea agglomerans SWg2 colonizes mulberry tissues,promotes disease protection and seedling growth[J].Biological Control,2017,113:9.DOI:10.1016/j.biocontrol.2017.06.010.
[6]YOON S H,KIM J E,LEE S H,et al.Engineering the lycopene synthetic pathway in E.coli by comparison of the carotenoid genes of Pantoea agglomerans and Pantoea ananatis[J].Applied Microbiology and Biotechnology,2007,74(1):131.DOI:10.1007/s00253-006-0623-z.
[7]WRIGHT S A,ZUMOFF C H,SCHNEIDER L,et al.Pantoea agglomerans strain EH318 produces two antibiotics that inhibit Erwinia amylovora in vitro[J].Applied and environmental microbiology,2001,67(1):284.DOI:10.1128/AEM.67.1.284-292.2001.
[8]WALTERSON A M,STAVRINIDES J,ROELOF V DM J.Pantoea:insights into a highly versatile and diverse genus within the Enterobacteriaceae[J].FEMS Microbiology Reviews,2015,39(6):968.DOI:10.1093/femsre/fuv027.
[9]黄敏.肺炎克雷伯氏菌KpC4菌株(Klebsiella pneumoniae KpC4)引起玉米细菌性顶腐病的病原学研究[D].昆明:云南农业大学,2017.
[10]贾鲁娜,潘立晶,陈铭德.细菌DNA紫外线辐射损伤和DNA修复系统的实验探究活动[J].生物学教学,2009,34(1):56.DOI:10.3969/j.issn.1004-7549.2009.01.031.
[11]李永利.球孢白僵菌可湿性粉剂的研制及土壤环境对其宿存的影响[D].保定:河北农业大学,2012.
[12]康萍芝,张丽荣,白小军,等.土壤环境因素对黄瓜枯萎病菌存活和生长繁殖的影响[J].宁夏农林科技,2017,58(10):32.DOI:10.3969/j.issn.1002-204X.2017.10.016.
[13]谷祖敏,王芳,纪明山,等.拮抗细菌B9对灰霉病菌的影响及在番茄叶表的定殖[J].云南农业大学学报,2006,21(6):842.DOI:10.3969/j.issn.1004-390X.2006.06.030.
[14]VORWERK S,MARTINEZ-TORRES D,FORNECK A.Pantoea agglomerans-associated bacteria in grape phylloxera(Daktulosphaira vitifoliae,Fitch)[J].Agricultural and Forest Entomology,2007,9(1):57.DOI:10.1111/j.1461-9563.2006.000319.x.
[15]JOHNSON K B,STOCKWELL V O,SAWYER T L,et al.Assessment of environmental factors influencing growth and spread of Pantoea agglomerans on and among blossoms of pear and apple[J].Phytopathology,2000,90(11):1285.DOI:10.1094/PHYTO.2000.90.11.1285.
[16]BARASH I,MANULIS-SASSON S.Recent evolution of bacterial pathogens:the gall-forming Pantoea agglomerans case[J].Annual Review of Phytopathology,2009,47:133.DOI:10.1146/annurev-phyto-080508-081803.
[17]MORALES-VALENZUELA G,SILVA-ROJAS H V,OCHOA-MARTINEZ D.First report of Pantoea agglomerans causing leaf blight and vascular wilt in maize and sorghum in Mexico[J].Journal of Plant Diseases and Protection,2017,91(10):1365.DOI:10.1094/PDIS-91-10-1365A.
[18]曹慧英.玉米新病害-细菌干茎腐病的研究[D].北京:中国农业科学院,2010.
[19]杨君洋,卢洪洲.成团泛菌感染及其诊治的研究近况[J].上海医药,2016,37(3):48.
[20]CANAMAS T P,VINAS I,USALL J,et al.Control of postharvest diseases on citrus fruit by preharvest application of the biocontrol agent Pantoea agglomerans CPA-2:Part I.Study of different formulation strategies to improve survival of cells in unfavourable environmental conditions[J].Postharvest Biology and Technology,2008,49(1):86.DOI:10.1016/j.postharvbio.2007.12.006.
[21]王洪媛.抗UV-B辐射菌Pantoea agglomerans KFS-9的选育及其抗辐射机制的研究[D].青岛:中国海洋大学,2006.
[22]REZZONICO F,SMITS T H M,MONTESIONS E,et al.Genotypic comparison of Pantoea agglomerans plant and clinical strains[J].BMC microbiology,2009,9(1):204.DOI:10.1186/1471-2180-9-204.
[23]SELVAKUMAR G,KUNDU S,JOSHI P,et al.Characterization of a cold-tolerant plant growth-promoting bacterium Pantoea dispersa 1A isolated from a sub-alpine soil in the North Western Indian Himalayas[J].World Journal of Microbiology and Biotechnology,2008,24(7):955.DOI:10.1007/s11274-007-9558-5.
[24]WENNEKER M,VERDEL M S W,GROENEVELD RM W,et al.Ralstonia(Pseudomonas)solanacearum race3(biovar 2)in surface water and natural weed hosts:first report on stinging nettle(Urtica dioica)[J].European Journal of Plant Pathology,1999,105(3):307.DOI:10.1111/j.1439-0434.1999.tb03854.x.
[25]PRADHANANG P M,ELPHINSTONE J G,FOX R TV.Identification of crop and weed hosts of Ralstonia solanacearum biovar 2 in the hills of Nepal[J].Plant Pathology,2000,49(4):403.DOI:10.1046/j.1365-3059.2000.00480.x.
[26]COUTINHO T A,VENTER S N.Pantoea ananatis:an unconventional plant pathogen[J].Molecular Plant Pathology,2009,10(3):325.DOI:10.1111/j.1364-3703.2009.00542.x.
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