菊芋应答非生物逆境胁迫的生长发育及物质代谢研究进展
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摘要
干旱缺水、土壤盐渍化等非生物胁迫已经成为中国许多地区农业生产发展的主要限制因素,直接影响着作物的生长状况。菊芋作为多年生草本植物,具有极强的抗逆性。在水分胁迫及盐碱胁迫下,菊芋的株高、基径、叶面积、和分枝数均表现出明显抑制现象。部分金属离子对菊芋的生长发育表现为一定程度的抑制作用,如Mg、Cu、Zn、Al和Hg,但是部分金属表现出对于菊芋生长不同的作用。在断根,摘叶以及去花的处理下,均表现出对菊芋产量增加的效应。光照时间的延长和缩短对菊芋的生长有不同的影响,同时研究还发现水杨酸可以缓解盐胁迫对菊芋的抑制作用,还能促进菊芋对K~+和Ca~(2+)的吸收和向上运输。研究还发现了HtCPI基因参与了菊芋对生物或非生物胁迫的防御机制。菊芋应答非生物胁迫的分子机理的研究目前并不多见,这将是菊芋应答非生物胁迫新的研究方向。
Abiotic s tress, such as drought, water shortage and soil salinization, has become the main limiting factor of agricultural production development in many areas of China, which directly affects the growth of crops.Jerusalem artichoke, a perennial herbaceous plant, has a strong adversity resistance. The plant height, plant base diameter, leaf area and tiller number of Jerusalem artichoke were significantly inhibited under stress of water and salt. Some metal ions inhibited the growth and development of Jerusalem artichoke to a certain extent, such as Mg,Cu, Zn, Al and Hg, while some metal ion showed different effects on the growth of Jerusalem artichoke. Under the treatment of root cutting, leaf picking and flower removal, the yield of Jerusalem artichoke increased. The prolongation and shortening of light time had different effects on the growth of Jerusalem artichoke. At the same time, it was found that salicylic acid(SA) could relieve the inhibition of salt stress on Jerusalem artichoke and promote the absorption and upward transport of K~+ and Ca~(2+) in Jerusalem artichoke. The study also found that the HtCPI gene was involved in the defense mechanism of Jerusalem artichoke on biological or abiotic stress. The research on the molecular mechanism of Jerusalem artichoke responding to abiotic stress is rare, and this would be the new research direction for the response of Jerusalem artichoke to abiotic stress.
Abiotic s tress, such as drought, water shortage and soil salinization, has become the main limiting factor of agricultural production development in many areas of China, which directly affects the growth of crops.Jerusalem artichoke, a perennial herbaceous plant, has a strong adversity resistance. The plant height, plant base diameter, leaf area and tiller number of Jerusalem artichoke were significantly inhibited under stress of water and salt. Some metal ions inhibited the growth and development of Jerusalem artichoke to a certain extent, such as Mg,Cu, Zn, Al and Hg, while some metal ion showed different effects on the growth of Jerusalem artichoke. Under the treatment of root cutting, leaf picking and flower removal, the yield of Jerusalem artichoke increased. The prolongation and shortening of light time had different effects on the growth of Jerusalem artichoke. At the same time, it was found that salicylic acid(SA) could relieve the inhibition of salt stress on Jerusalem artichoke and promote the absorption and upward transport of K~+ and Ca~(2+) in Jerusalem artichoke. The study also found that the HtCPI gene was involved in the defense mechanism of Jerusalem artichoke on biological or abiotic stress. The research on the molecular mechanism of Jerusalem artichoke responding to abiotic stress is rare, and this would be the new research direction for the response of Jerusalem artichoke to abiotic stress.
引文
Cao L.,Wu Y.H.,Zhang Y.,Guo Y.,Xiao Y.T.,Li F.,Ma L.,and Xu G.D.,2015,Effect of exogenous salicylic acid on photosynthetic characteristics and aluminum tolerance of Helianthus tuberosus L.under aluminum stress,Shuitu Baochi Xuebao(Journal of Soil and Water Conservation),4(29):260-266(曹林,吴玉环,章艺,郭怡,肖有铁,郦枫,马丽,徐根娣,刘鹏,2015,外源水杨酸对铝胁迫下菊芋光合特性及耐铝性的影响,水土保持学报,4(29):260-266)
Chen J.R.,2017,Effects of different environmental factors on the growth and reproductive strategies of Jerusalem artichoke,Thesis for M.S.,Lanzhou University,Supervisor:Yuan J.L.,pp.11-20(陈荣健,2017,不同环境因素对菊芋生长和繁殖策略的影响,硕士学位论文,兰州大学,导师:袁建立,pp.11-20)
Chen L.,2011,Responses of two jerusalem artichoke(Helianthus tuberosus L.)cultivars seedling to cadmium stress and the mitigation effects of exogenous of salicylic acid,Thesis for M.S.,Nanjing Agricultural University,Supervisor:Liu Z.P.,Long X.H.,pp.19-51(陈良,2011,2种菊芋幼苗对锡胁迫的响应及外源水杨酸的缓解作用,硕士学位论文,南京农业大学,导师:刘兆普,隆小华,pp.19-51)
Ding T.F.,2017,The effect of photoperiod variation on growth of Jerusalem artichoke,Thesis for M.S.,Lanzhou University,Supervisor:Zhao Z.G.,pp.46-50(丁天凤,2017,光周期处理对菊芋生长的影响,硕士学位论文,兰州大学,导师:赵志刚,pp.46-50)
Huang Z.R.,Long X.H.,Li H.Y.,Xin B.R.,Li Q.,Liu L.,and Liu Z.P.,2010,Coupling effect of salt and fertilizer application on Helianthus tuberosus L.in soils of North Jiangsu coastal mudflat different in salt concentrat,Turang Xuebao(Acta Pedologica Sinica),4(47):709-714(黄增荣,隆小华,李洪燕,辛本荣,李青,刘玲,刘兆普,2010,江苏北部滨海盐土盐肥耦合对菊芋生长和产量的影响,土壤学报,4(47):709-714)
Jia R.L.,2012,Effects of Cu Treatmenton physiological and biochemical characters of erusalem artichoke(Helianthus tuberosus)leaves,41(8):154-156(贾若凌,2012,铜胁迫对菊芋幼叶生理生化指标的影响,河南农业科学,41(8):154-156)
Li H.,2014,The effect of defloration on Jerusalem artichoke growth and clone and expression parttern of HTNF-YBfamily,Thesis for M.S.,Nanjing Agricultural University,Supervisors:Zhao G.M.,and Liang M.X.,pp.13-24(李辉,2014,菊芋去花对菊芋生长发育的影响以及对参与生长阶段调控基因NF-YB家族的克隆和表达分析,硕士学位论文,南京农业大学,导师:赵耕毛,梁明祥,pp.13-24)
Li H.,Kang J.,Zhao G.M.,Yin X.M.,and Liang M.X.,2014,Effects of salinity on accumulation and distribution mode of dry matter and soluble sugar of Jerusalem artichoke(Helianthus tuberosus L.),Caoye Xuebao(Acta Prataculturae Sinica),2(23):160-170(李辉,康健,赵耕毛,尹晓明,梁明祥,2014,盐胁迫对菊芋干物质和糖分积累分配的影响,草业学报,2(23):160-170)
Liu H.,Teng A.D.,Wang L.,Chu W.K.,and Gao K.,2017,The influence of defloration on tuber biomass and matter allocation in Jerusalem artichoke,Caoyuan Yu Caoye(Grassland And Prataculture),1(29):43-48(刘辉,腾爱娣,王琳,初文凯,高凯,2017,去花对菊芋块茎产量及物质分配规律的相关性研究,草原与草业,1(29):43-48)
Liu H.,Teng A.D.,Wang L.,Deng B.,and Gao K.,2016,Effection of defoliation on biomass,height and growth rate of Helianthus tuberosus L.,Caodi Xuebao(Acta Agrestia Sinica),4(24):915-918(刘辉,滕爱娣,王琳,邓波,高凯,2016,去叶对不同时期菊芋生物量,株高和生长速率的影响,草地学报,4(24):915-918)
Liu P.,Li F.,Cao L.,Wu Y.H.,Zhang Y.,Xu G.D.,Ma L.,and Liu D.,2017,Cloning and expression analysis of HtCPIgene with the evaluation of the stress resistance in Helianthus tuberosus L.,Zhejiang Shifan Daxue Xuebao(Journal of Zhejiang Normal University),2(40):188-195(刘鹏,郦枫,曹林,吴玉环,章艺,徐根娣,马丽,刘丹,2017,菊芋抗逆性评估及HtCPI基因的克隆与表达分析,浙江师范大学学报,2(40):188-195)
Long X.H.,Liu Z.P.,Liu L.,Chen M.D.,and Zheng Q.S.,2004,Effects of different concentrations of seawater on growths,developments and absorption of P of Helianthus tuberosus L.seedlings,Zhiwu Yanjiu(Bulletin of Botanical Research),24(3):331-334(隆小华,刘兆普,刘玲,陈铭达,郑青松,2004,不同浓度海水胁迫对菊芋幼苗生长发育及磷吸收的影响,植物研究,24(3):331-334)
LüS.Q.,2015,The effects of soil mercury stress on physiological and biochemicanl characteristics of Jerusalem arichoke,Dissertation for Ph.D.,Lanzhou University,Supervisor:Zhao C.M.,pp.16-31(吕世奇,2015,土壤汞污染对菊芋生理生化特性影响,博士学位论文,兰州大学,导师:赵长明,pp.16-31)
Shao S.,2015,Response of Jerusalem artichoke(Helianthus tuberosus L.)to soil stress and exogenous nitrogen regulation effects,Dissertation for Ph.D.,Northeast Forestry University,Supervisor:Yan X.F.,pp.20-27(邵帅,2015,菊芋对土壤逆境胁迫的响应及氮素的调控效应硏究,博士学位论文,东北林业大学,导师:阎秀峰,pp.20-27)
Sun X.E.,Liu Z.P.,and Long X.H.,2012,Effects of different levels of magnesium supply on the seedling's growth,photosynthesis,and chlorophyll fluorescence characteristics of two Helianthus tuberous L.varieties,Shengtaixue Zazhi(Chinese Journal of Ecology),31(4):823-829(孙晓娥,刘兆普,隆小华,2012,不同供镁水平对两菊芋品种幼苗生物量,光合和叶绿素荧光特性的影响,生态学杂志,31(4):823-829)
Wang L.,Long X.H.,Meng X.F.,Liu Z.P.,and Jin L.,2011,Effects of salicylic acid on photosynthesis and ion absorption Helianthus tuberosus L.seedlings under NaCl stress.,Shengtaixue Zazhi(Chinese Journal of Ecology),30(9):1901-1907(王磊,隆小华,孟宪法,刘兆普,晋利,2011,水杨酸对NaCl胁迫下菊芋幼苗光合作用及离子吸收的影响,生态学杂志,30(9):1901-1907)
Wu R.N.,2015,The effect of root cutting on tuber biomass and quality of Helianthus tuberosus L.,Thesis for M.S.,Inner Mongolia Agricultural University,Supervisor:Gao K.,pp.7-25(乌日娜,2015,根系切割对菊芋块茎生物产量及品质的影响,硕士学位论文,内蒙古农业大学,导师:高凯,pp.7-25)
Xue Y.F.,and Liu Z.P.,2007,Effect of exogenous calcium on alleviation of the decreased photosynthetic ability of Helianthus tuberosus L.leaves under seawater stress,Caoye Xuebao(Acta Prataculturae Sinica),6(16):74-80(薛延丰,刘兆普,2007,外源钙离子缓解海水胁迫下菊芋光合能力下降的研究,草业学报,6(16):74-80)
Yang B.,2010,Effect of Non-full Irrigation on ecological characteristics related to water and photosynthesis and crop productivity of Jerusalem artichoke,Thesis for M.S.,Gansu Agricultural University,Supervisor:Zhang H.J.,pp.16-20(杨斌,2010,非充分灌溉对菊芋水分光合生态特征及生产力的影响,硕士学位论文,甘肃农业大学,导师:张恒嘉,pp.16-20)
Zhang G.X.,Hao G.Q.,Liu Y.H.,Xue Z.Z.,Wang X.P.,and Wu X.H.,2014,The effects of salt tolerance on growth index of Helianthus tuberosus L.seedling,Hebei Nongye Kexue(Journal of Hebei Agricultural Sciences),18(3):13-16(张国新,郝桂琴,刘雅辉,薛志忠,王秀萍,吴新海,2014,盐分胁迫对菊芋幼苗生长指标的影响,河北农业科学,18(3):13-16)
Zhao M.L.,Liu S.Y.,and Li L.,2012,The comparative study of Jerusalem artichoke L.Tubers on different treament of breaking dormancy,Zhongzi(Seed),7(31):47-50(赵孟良,刘素英,李莉,2012,不同处理方法打破菊芋块茎休眠对比研究,种子,7(31):47-50)
Zhong Q.W.,Wang Y.,Wang L.H.,and Li L.,2007,Changes of growth,development and photosynthesis indicators of Jerusalem artichoke,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),27(9):1843-1848(钟启文,王怡,王丽慧,李莉,2007,菊芋生长发育动态及光合性能指标变化研究,西北植物学报,27(9):1843-1848)
Zhu J.H.,2016,Salt-tolerant adaption of Jerusalem artichoke plant based on the physiolgical and proteomic analysis,Thesis for M.S.,Nanjing Agricultural University,Supervisor:Zhao G.M.,pp.47-68(朱菊华,2016,基于生理与蛋白质组分析的菊芋耐盐适应性研究,硕士学位论文,南京农业大学,导师:赵耕毛,pp.47-68)
Zhu T.X.,Men G.T.,Wang L.,Wu R.N.,and Gao K.,2016a,The effect of cutting root on quantitative traits and quality traits in Helianthus tuberosus L.,Caodi Xuebao(Acta Agrestia Sinica),5(24):1055-1061(朱铁霞,门果桃,王琳,乌日娜,高凯,2016a,断根对菊芋数量性状和质量性状的影响,草地学报,5(24):1055-1061)
Zhu T.X.,Wang L.,Gao Y.,Men G.T.,Wu R.N.,and Gao K.,2016b,Effect of root cutting on growth rate and matter accumulation tate in Helianthus tuberosus L.,Beifang Yuanyi(Northern Horticulture),6(21):28-31(朱铁霞,王琳,高阳,门果桃,乌日娜,高凯,2016b,断根对菊芋生长速率和物质积累速率的影响,北方园艺,6(21):28-31)
Chen J.R.,2017,Effects of different environmental factors on the growth and reproductive strategies of Jerusalem artichoke,Thesis for M.S.,Lanzhou University,Supervisor:Yuan J.L.,pp.11-20(陈荣健,2017,不同环境因素对菊芋生长和繁殖策略的影响,硕士学位论文,兰州大学,导师:袁建立,pp.11-20)
Chen L.,2011,Responses of two jerusalem artichoke(Helianthus tuberosus L.)cultivars seedling to cadmium stress and the mitigation effects of exogenous of salicylic acid,Thesis for M.S.,Nanjing Agricultural University,Supervisor:Liu Z.P.,Long X.H.,pp.19-51(陈良,2011,2种菊芋幼苗对锡胁迫的响应及外源水杨酸的缓解作用,硕士学位论文,南京农业大学,导师:刘兆普,隆小华,pp.19-51)
Ding T.F.,2017,The effect of photoperiod variation on growth of Jerusalem artichoke,Thesis for M.S.,Lanzhou University,Supervisor:Zhao Z.G.,pp.46-50(丁天凤,2017,光周期处理对菊芋生长的影响,硕士学位论文,兰州大学,导师:赵志刚,pp.46-50)
Huang Z.R.,Long X.H.,Li H.Y.,Xin B.R.,Li Q.,Liu L.,and Liu Z.P.,2010,Coupling effect of salt and fertilizer application on Helianthus tuberosus L.in soils of North Jiangsu coastal mudflat different in salt concentrat,Turang Xuebao(Acta Pedologica Sinica),4(47):709-714(黄增荣,隆小华,李洪燕,辛本荣,李青,刘玲,刘兆普,2010,江苏北部滨海盐土盐肥耦合对菊芋生长和产量的影响,土壤学报,4(47):709-714)
Jia R.L.,2012,Effects of Cu Treatmenton physiological and biochemical characters of erusalem artichoke(Helianthus tuberosus)leaves,41(8):154-156(贾若凌,2012,铜胁迫对菊芋幼叶生理生化指标的影响,河南农业科学,41(8):154-156)
Li H.,2014,The effect of defloration on Jerusalem artichoke growth and clone and expression parttern of HTNF-YBfamily,Thesis for M.S.,Nanjing Agricultural University,Supervisors:Zhao G.M.,and Liang M.X.,pp.13-24(李辉,2014,菊芋去花对菊芋生长发育的影响以及对参与生长阶段调控基因NF-YB家族的克隆和表达分析,硕士学位论文,南京农业大学,导师:赵耕毛,梁明祥,pp.13-24)
Li H.,Kang J.,Zhao G.M.,Yin X.M.,and Liang M.X.,2014,Effects of salinity on accumulation and distribution mode of dry matter and soluble sugar of Jerusalem artichoke(Helianthus tuberosus L.),Caoye Xuebao(Acta Prataculturae Sinica),2(23):160-170(李辉,康健,赵耕毛,尹晓明,梁明祥,2014,盐胁迫对菊芋干物质和糖分积累分配的影响,草业学报,2(23):160-170)
Liu H.,Teng A.D.,Wang L.,Chu W.K.,and Gao K.,2017,The influence of defloration on tuber biomass and matter allocation in Jerusalem artichoke,Caoyuan Yu Caoye(Grassland And Prataculture),1(29):43-48(刘辉,腾爱娣,王琳,初文凯,高凯,2017,去花对菊芋块茎产量及物质分配规律的相关性研究,草原与草业,1(29):43-48)
Liu H.,Teng A.D.,Wang L.,Deng B.,and Gao K.,2016,Effection of defoliation on biomass,height and growth rate of Helianthus tuberosus L.,Caodi Xuebao(Acta Agrestia Sinica),4(24):915-918(刘辉,滕爱娣,王琳,邓波,高凯,2016,去叶对不同时期菊芋生物量,株高和生长速率的影响,草地学报,4(24):915-918)
Liu P.,Li F.,Cao L.,Wu Y.H.,Zhang Y.,Xu G.D.,Ma L.,and Liu D.,2017,Cloning and expression analysis of HtCPIgene with the evaluation of the stress resistance in Helianthus tuberosus L.,Zhejiang Shifan Daxue Xuebao(Journal of Zhejiang Normal University),2(40):188-195(刘鹏,郦枫,曹林,吴玉环,章艺,徐根娣,马丽,刘丹,2017,菊芋抗逆性评估及HtCPI基因的克隆与表达分析,浙江师范大学学报,2(40):188-195)
Long X.H.,Liu Z.P.,Liu L.,Chen M.D.,and Zheng Q.S.,2004,Effects of different concentrations of seawater on growths,developments and absorption of P of Helianthus tuberosus L.seedlings,Zhiwu Yanjiu(Bulletin of Botanical Research),24(3):331-334(隆小华,刘兆普,刘玲,陈铭达,郑青松,2004,不同浓度海水胁迫对菊芋幼苗生长发育及磷吸收的影响,植物研究,24(3):331-334)
LüS.Q.,2015,The effects of soil mercury stress on physiological and biochemicanl characteristics of Jerusalem arichoke,Dissertation for Ph.D.,Lanzhou University,Supervisor:Zhao C.M.,pp.16-31(吕世奇,2015,土壤汞污染对菊芋生理生化特性影响,博士学位论文,兰州大学,导师:赵长明,pp.16-31)
Shao S.,2015,Response of Jerusalem artichoke(Helianthus tuberosus L.)to soil stress and exogenous nitrogen regulation effects,Dissertation for Ph.D.,Northeast Forestry University,Supervisor:Yan X.F.,pp.20-27(邵帅,2015,菊芋对土壤逆境胁迫的响应及氮素的调控效应硏究,博士学位论文,东北林业大学,导师:阎秀峰,pp.20-27)
Sun X.E.,Liu Z.P.,and Long X.H.,2012,Effects of different levels of magnesium supply on the seedling's growth,photosynthesis,and chlorophyll fluorescence characteristics of two Helianthus tuberous L.varieties,Shengtaixue Zazhi(Chinese Journal of Ecology),31(4):823-829(孙晓娥,刘兆普,隆小华,2012,不同供镁水平对两菊芋品种幼苗生物量,光合和叶绿素荧光特性的影响,生态学杂志,31(4):823-829)
Wang L.,Long X.H.,Meng X.F.,Liu Z.P.,and Jin L.,2011,Effects of salicylic acid on photosynthesis and ion absorption Helianthus tuberosus L.seedlings under NaCl stress.,Shengtaixue Zazhi(Chinese Journal of Ecology),30(9):1901-1907(王磊,隆小华,孟宪法,刘兆普,晋利,2011,水杨酸对NaCl胁迫下菊芋幼苗光合作用及离子吸收的影响,生态学杂志,30(9):1901-1907)
Wu R.N.,2015,The effect of root cutting on tuber biomass and quality of Helianthus tuberosus L.,Thesis for M.S.,Inner Mongolia Agricultural University,Supervisor:Gao K.,pp.7-25(乌日娜,2015,根系切割对菊芋块茎生物产量及品质的影响,硕士学位论文,内蒙古农业大学,导师:高凯,pp.7-25)
Xue Y.F.,and Liu Z.P.,2007,Effect of exogenous calcium on alleviation of the decreased photosynthetic ability of Helianthus tuberosus L.leaves under seawater stress,Caoye Xuebao(Acta Prataculturae Sinica),6(16):74-80(薛延丰,刘兆普,2007,外源钙离子缓解海水胁迫下菊芋光合能力下降的研究,草业学报,6(16):74-80)
Yang B.,2010,Effect of Non-full Irrigation on ecological characteristics related to water and photosynthesis and crop productivity of Jerusalem artichoke,Thesis for M.S.,Gansu Agricultural University,Supervisor:Zhang H.J.,pp.16-20(杨斌,2010,非充分灌溉对菊芋水分光合生态特征及生产力的影响,硕士学位论文,甘肃农业大学,导师:张恒嘉,pp.16-20)
Zhang G.X.,Hao G.Q.,Liu Y.H.,Xue Z.Z.,Wang X.P.,and Wu X.H.,2014,The effects of salt tolerance on growth index of Helianthus tuberosus L.seedling,Hebei Nongye Kexue(Journal of Hebei Agricultural Sciences),18(3):13-16(张国新,郝桂琴,刘雅辉,薛志忠,王秀萍,吴新海,2014,盐分胁迫对菊芋幼苗生长指标的影响,河北农业科学,18(3):13-16)
Zhao M.L.,Liu S.Y.,and Li L.,2012,The comparative study of Jerusalem artichoke L.Tubers on different treament of breaking dormancy,Zhongzi(Seed),7(31):47-50(赵孟良,刘素英,李莉,2012,不同处理方法打破菊芋块茎休眠对比研究,种子,7(31):47-50)
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