云南省38对HIV-1双阳性夫妻的分子流行病学特征分析
|
摘要
目的分析云南省38对人免疫缺陷病毒1型(human immunodeficiency virus type 1, HIV-1)双阳性夫妻的分子流行病学特征。方法收集云南省传染病专科医院检验科2014-2016年HIV-1抗体初筛及确证阳性的样本及实验室资料,筛选出41对双阳性夫妻,采用逆转录套式聚合酶链反应(reverse transcription nested polymerase chain reaction,RT-nested-PCR)扩增后测序,将序列进行比对拼接,运用合格的pol基因区序列构建系统进化树并分析亚型,并计算每对双阳夫妻间序列的基因距离差异。结果共获得38对双阳性夫妻pol基因区序列76条,主要基因亚型为CRF08_BC(45,59.2%)、CRF07_BC(18,23.7%)和CRF01_AE(7,9.2%),另外还有C(4,5.3%)和B(2,2.6%)。系统进化树分析及基因距离计算可明确有9对双阳夫妻为夫妻间传播。结论随着HIV性传播比例的逐年上升,夫妻间传播应当成为重点关注的问题,HIV感染的晚发现是造成夫妻间传播的主要因素,对于特殊人群应及早加强监测及检测,减少艾滋病夫妻间的传播的发生。
Objective To analyze the molecular epidemiological characteristics of 38 HIV-1 positive seroconcordant couples in Yunnan Province. Methods The samples of HIV-1 antibody screened initially and positive confirmed in the Laboratory of Yunnan Provincial Infectious Diseases Specialist Hospital from 2014 to 2016 and laboratory data were collected. 41 samples from HIV-1 positive seroconcordant couples were selected and sequenced by RT-nested-PCR amplification. After the sequences were aligned and spliced, the qualified pol gene region sequence was used to construct phylogenetic tree and analyze subtypes, and the gene distance differences between each positive couple were calculated. Results A total of 76 pol gene regions from 38 HIV-1 positive seroconcordant couples were obtained. The major gene subtypes included CRF08_BC(45,59.2%), CRF07_BC(18,23.7%) and CRF01_AE(7,9.2%), there were also C(4, 5.3%) and B(2, 2.6%). Phylogenetic tree analysis and gene distance calculation clearly showed that HIV transmission between relationship partners occurred in 9 HIV-1 positive seroconcordant couples. Conclusions As the proportion of sexual transmission of HIV has risen year by year, the transmission between husband and wife should be the focus of our attention. Late diagnosis of HIV infection is the main factor that causes the transmission between husband and wife. For special populations, monitoring and testing should be strengthened as soon as possible, so as to reduce the occurrence of transmission between AIDS couples.
Objective To analyze the molecular epidemiological characteristics of 38 HIV-1 positive seroconcordant couples in Yunnan Province. Methods The samples of HIV-1 antibody screened initially and positive confirmed in the Laboratory of Yunnan Provincial Infectious Diseases Specialist Hospital from 2014 to 2016 and laboratory data were collected. 41 samples from HIV-1 positive seroconcordant couples were selected and sequenced by RT-nested-PCR amplification. After the sequences were aligned and spliced, the qualified pol gene region sequence was used to construct phylogenetic tree and analyze subtypes, and the gene distance differences between each positive couple were calculated. Results A total of 76 pol gene regions from 38 HIV-1 positive seroconcordant couples were obtained. The major gene subtypes included CRF08_BC(45,59.2%), CRF07_BC(18,23.7%) and CRF01_AE(7,9.2%), there were also C(4, 5.3%) and B(2, 2.6%). Phylogenetic tree analysis and gene distance calculation clearly showed that HIV transmission between relationship partners occurred in 9 HIV-1 positive seroconcordant couples. Conclusions As the proportion of sexual transmission of HIV has risen year by year, the transmission between husband and wife should be the focus of our attention. Late diagnosis of HIV infection is the main factor that causes the transmission between husband and wife. For special populations, monitoring and testing should be strengthened as soon as possible, so as to reduce the occurrence of transmission between AIDS couples.
引文
[1] 郝阳,崔岩,孙新华,等. “四免一关怀”政策实施十年来中国艾滋病疫情变化及特征分析 [J]. 中华疾病控制杂志, 2014,18(5):369-374. Hao Y, Cui Y, Sun XH, et al. A retrospective study of HIV/AIDS situation: a ten-year implementation of “four frees and one care” policy in China [J]. Chin J Dis Control Prev, 2014,18(5):369-374.
[2] 端琼丽,张维义,常铃,等. 云南省红河州HIV抗体双阳夫妻的流行病学分析 [J]. 中国艾滋病性病, 2016,22(4):260-262. DOI:10.13419/j.cnki.aids.2016.04.11. Duan QL, Zhang WY, Chang L, et al. Analysis of epidemiological characteristics among HIV-1positive couples in Honghe Prefecture,Yunnan Province, China [J]. Chin J AIDS STD, 2016,22(4):260-262. DOI:10.13419/j.cnki.aids.2016.04.11.
[3] 钟敏,李韩平,李惠琴,等. 云南省2012年人类免疫缺陷病毒感染者和艾滋病患者抗病毒治疗失败基因型耐药情况分析 [J]. 中华传染病杂志, 2015,33(1):30-33. DOI:10.3760/cma.j.issn.1000-6680.2015.01.008. Zhong M, Li HP, Li HQ, et al. Analysis of human immunodeficiency virus genotypic drug resistance among human immunodeficiency virus infection and acquired immune deficiency syndrome individuals with virological failure after free antiretroviral therapy in Yunnan Province in 2012 [J]. Chin J Infect Dis, 2015,33(1):30-33. DOI:10.3760/cma.j.issn.1000-6680.2015.01.008.
[4] 时丽丽,赵琦,温玉洁,等. HIV暴露后感染分子溯源技术的研究进展 [J]. 中国艾滋病性病, 2015,21(8):746-748. DOI:10.13419/j.cnki.aids.2015.08.31. Shi LL, Zhao Q, Wen YJ, et al. A review on the molecular tracing approaches for the identification of postexposure HIV transmissio [J]. Chin J AIDS STD, 2015,21(8):746-748. DOI:10.13419/j.cnki.aids.2015.08.31.
[5] Ou CY, Ciesielski CA, Myers G, et al. Molecular epidemiology of HIV transmission in a dental practice [J]. Science, 1992,256(5060):1165-1171. DOI:10.1126/science.256.5060.1165.
[6] Highoter GK, May SJ, Perez-Santiago J, et al. HIV-1 clade B Pol evolution following primary infection [J]. PLoS One, 2013,8(6):e68188. DOI:10.1371/journal.pone.0068188.
[7] 陈方方,郭巍,王丽艳,等. 我国部分地区艾滋病非婚异性性传播病例感染方式构成及特征分析 [J]. 中国艾滋病性病, 2015,21(7):550-553. DOI:10.13419/j.cnki.aids.2015.07.02. Chen FF, Guo W, Wang LY, et al. Characteristics of HIV/AIDS cases with extra-marital heterosexual transmission in some regions in China [J]. Chin J AIDS STD, 2015,21(7):550-553. DOI:10.13419/j.cnki.aids.2015.07.02.
[8] Mehra B, Bhalla P, Rawat D, et al. A study of HIV-concordant and discordant couples Attending voluntary counselling and testing services at a tertiary care center In North India [J]. Indian J Public Health, 2015,59(4):306-309. DOI:10.4103/0019-557X.169664.
[9] 项丽芬,高洁,刘春馨,等. 云南省德宏州利用PITC策略发现的HIV感染者特征及卫生服务利用分析 [J]. 中华疾病控制杂志, 2016,20(4):341-344. DOI:10.16462/j.cnki.zhjbkz.2016.04.005. Xiang LF, Gao J, Liu CX, et al. Characteristics and health service utilization of HIV positive individuals detected through free PITC strategy in Dehong prefecture [J]. Chin J Dis Control Prev, 2016,20(4):341-344. DOI:10.16462/j.cnki.zhjbkz.2016.04.005.
[10] Liu J, Zhang CY. Phylogeographic analyses reveal a crucial role of Xinjiang in HIV-1CRF07_BC and HCV 3a transmissions in Asia [J]. PLoS One, 2011,6(8):e23347. DOI:10.1371/journal.pone.0023347.
[11] 王炳辉. 云南省HIV-1跨境传播特性及亚型演化规律研究 [D]. 昆明:昆明理工大学, 2016. Wang BH. Study on HIV-1 cross-border transmission and evolutionary）characteristics of its subtypes in Yunnan Province, China [D]. Kunming:Kunming University of Science, 2016.
[12] Cheng H, Zhang J, Capizzi J, et al. HIV-1 subtype E in Yunnan, China [J]. Lancet, 1994,344(8927):953-954. DOI:10.1016/S0140-6736(94)92304-3.
[13] Luo CC, Tiam C, Hu DJ, et al. HIV-1 subtype C in China [J]. Lancet, 1995,345(8956):1051-1052.
[14] Weniger BG, Takebe Y, Ou CY, et al. The molecular epidemiology of HIV in Asia [J]. AIDS, 1994,8(Suppl 2):S13-S28.
[15] 艾滋病防治条例(国务院令第457号) [Z]. 2006. AIDS Prevention Act (Decree No.457 of the State Council of the People's Republic of China) [Z]. 2006.
[2] 端琼丽,张维义,常铃,等. 云南省红河州HIV抗体双阳夫妻的流行病学分析 [J]. 中国艾滋病性病, 2016,22(4):260-262. DOI:10.13419/j.cnki.aids.2016.04.11. Duan QL, Zhang WY, Chang L, et al. Analysis of epidemiological characteristics among HIV-1positive couples in Honghe Prefecture,Yunnan Province, China [J]. Chin J AIDS STD, 2016,22(4):260-262. DOI:10.13419/j.cnki.aids.2016.04.11.
[3] 钟敏,李韩平,李惠琴,等. 云南省2012年人类免疫缺陷病毒感染者和艾滋病患者抗病毒治疗失败基因型耐药情况分析 [J]. 中华传染病杂志, 2015,33(1):30-33. DOI:10.3760/cma.j.issn.1000-6680.2015.01.008. Zhong M, Li HP, Li HQ, et al. Analysis of human immunodeficiency virus genotypic drug resistance among human immunodeficiency virus infection and acquired immune deficiency syndrome individuals with virological failure after free antiretroviral therapy in Yunnan Province in 2012 [J]. Chin J Infect Dis, 2015,33(1):30-33. DOI:10.3760/cma.j.issn.1000-6680.2015.01.008.
[4] 时丽丽,赵琦,温玉洁,等. HIV暴露后感染分子溯源技术的研究进展 [J]. 中国艾滋病性病, 2015,21(8):746-748. DOI:10.13419/j.cnki.aids.2015.08.31. Shi LL, Zhao Q, Wen YJ, et al. A review on the molecular tracing approaches for the identification of postexposure HIV transmissio [J]. Chin J AIDS STD, 2015,21(8):746-748. DOI:10.13419/j.cnki.aids.2015.08.31.
[5] Ou CY, Ciesielski CA, Myers G, et al. Molecular epidemiology of HIV transmission in a dental practice [J]. Science, 1992,256(5060):1165-1171. DOI:10.1126/science.256.5060.1165.
[6] Highoter GK, May SJ, Perez-Santiago J, et al. HIV-1 clade B Pol evolution following primary infection [J]. PLoS One, 2013,8(6):e68188. DOI:10.1371/journal.pone.0068188.
[7] 陈方方,郭巍,王丽艳,等. 我国部分地区艾滋病非婚异性性传播病例感染方式构成及特征分析 [J]. 中国艾滋病性病, 2015,21(7):550-553. DOI:10.13419/j.cnki.aids.2015.07.02. Chen FF, Guo W, Wang LY, et al. Characteristics of HIV/AIDS cases with extra-marital heterosexual transmission in some regions in China [J]. Chin J AIDS STD, 2015,21(7):550-553. DOI:10.13419/j.cnki.aids.2015.07.02.
[8] Mehra B, Bhalla P, Rawat D, et al. A study of HIV-concordant and discordant couples Attending voluntary counselling and testing services at a tertiary care center In North India [J]. Indian J Public Health, 2015,59(4):306-309. DOI:10.4103/0019-557X.169664.
[9] 项丽芬,高洁,刘春馨,等. 云南省德宏州利用PITC策略发现的HIV感染者特征及卫生服务利用分析 [J]. 中华疾病控制杂志, 2016,20(4):341-344. DOI:10.16462/j.cnki.zhjbkz.2016.04.005. Xiang LF, Gao J, Liu CX, et al. Characteristics and health service utilization of HIV positive individuals detected through free PITC strategy in Dehong prefecture [J]. Chin J Dis Control Prev, 2016,20(4):341-344. DOI:10.16462/j.cnki.zhjbkz.2016.04.005.
[10] Liu J, Zhang CY. Phylogeographic analyses reveal a crucial role of Xinjiang in HIV-1CRF07_BC and HCV 3a transmissions in Asia [J]. PLoS One, 2011,6(8):e23347. DOI:10.1371/journal.pone.0023347.
[11] 王炳辉. 云南省HIV-1跨境传播特性及亚型演化规律研究 [D]. 昆明:昆明理工大学, 2016. Wang BH. Study on HIV-1 cross-border transmission and evolutionary）characteristics of its subtypes in Yunnan Province, China [D]. Kunming:Kunming University of Science, 2016.
[12] Cheng H, Zhang J, Capizzi J, et al. HIV-1 subtype E in Yunnan, China [J]. Lancet, 1994,344(8927):953-954. DOI:10.1016/S0140-6736(94)92304-3.
[13] Luo CC, Tiam C, Hu DJ, et al. HIV-1 subtype C in China [J]. Lancet, 1995,345(8956):1051-1052.
[14] Weniger BG, Takebe Y, Ou CY, et al. The molecular epidemiology of HIV in Asia [J]. AIDS, 1994,8(Suppl 2):S13-S28.
[15] 艾滋病防治条例(国务院令第457号) [Z]. 2006. AIDS Prevention Act (Decree No.457 of the State Council of the People's Republic of China) [Z]. 2006.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |