基于化学成分和药理效应比较益母草的传统加工工艺与一体化加工工艺

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英文篇名：Comparison Between Traditional and Integrated Processing Technology of Leonuri Herba Based on Chemical Composition and Pharmacological Effect 作者：乔晶晶 ; 吴啟南 ; 薛敏 ; 王倩 ; 徐安娜 ; 吴达维 英文作者：QIAO Jing-jing;WU Qi-nan;XUE Min;WANG Qian;XU An-na;WU Da-wei;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,College of Pharmacy,Nanjing University of Chinese Medicine; 关键词：益母草 ; 一体化加工 ; 生物碱 ; 黄酮类 ; 抗炎作用 ; 急性血瘀模型 ; 传统加工工艺 英文关键词：Leonuri Herba;;integrated processing;;alkaloids;;flavonoids;;anti-inflammatory;;acute blood stasis model;;traditional processing technology 中文刊名：ZSFX 英文刊名：Chinese Journal of Experimental Traditional Medical Formulae 机构：南京中医药大学药学院江苏省中药资源产业化过程协同创新中心中药资源产业化与方剂创新药物国家地方联合工程研究中心; 出版日期：2018-10-18 11:52 出版单位：中国实验方剂学杂志 年：2019 期：v.25 基金：国家中医药行业科研专项(2015468002-2);; 江苏高校优势学科建设工程项目(ysxk-2010);; 江苏省中药资源产业化过程协同创新中心项目(2011) 语种：中文; 页：ZSFX201908016 页数：7 CN：08 ISSN：11-3495/R 分类号：109-115

摘要

目的:比较益母草传统加工饮片与一体化加工饮片在化学成分含量与药理作用方面的差异,阐述益母草一体化加工工艺的合理性。方法:选择盐酸水苏碱、盐酸益母草碱、芦丁、金丝桃苷、异槲皮苷的含量为指标,比较2种加工工艺益母草饮片中化学成分含量的差异;以二甲苯致小鼠耳廓肿胀模型,考察2种加工工艺益母草饮片抗炎作用的差异;采用大鼠急性血瘀模型,考察2种加工工艺益母草饮片对全血黏度和凝血功能指标的影响。结果:益母草一体化工艺饮片中盐酸水苏碱、盐酸益母草碱、芦丁、金丝桃苷、异槲皮苷的质量分数分别为1. 558%,0. 168%,0. 137%,0. 113%,0. 078%;传统工艺饮片中则分别为1. 482%,0. 134%,0. 125%,0. 082%,0. 071%。2种工艺饮片均能降低致炎小鼠耳肿胀度、降低致炎小鼠血清中肿瘤坏死因子-α(TNF-α),白细胞介素(IL)-1β和IL-6的含量,产生抗炎作用;同剂量组间比较,一体化工艺组整体优于传统工艺组。2种加工饮片均能降低急性血瘀模型大鼠的全血黏度,延长凝血酶时间(TT),凝血酶原时间(PT)和活化部分凝血活酶时间(APTT),降低血浆纤维蛋白原(FIB)的质量浓度;同剂量组间比较,一体化工艺组整体优于传统工艺组。结论:与传统加工工艺相比,益母草一体化加工工艺在保证饮片质量的同时,降低了生产成本,且所得饮片化学成分与药理作用均略高于传统加工饮片,表明该药材选择一体化加工具有一定的合理性。
        Objective: To elucidate the rationality of integrated processing technology of Leonuri Herba based on comparison of chemical constituents and pharmacological effect of Leonuri Herba between traditional and integrated processing technology. Method: The contents of stachydrine hydrochloride,leonurine hydrochloride,rutin,hyperoside and isoquercetin were used as indexes to compare the differences in the contents of chemical constituents between traditional and integration processing technology of Leonuri Herba. Effect of Leonuri Herba with different processing technology on auricular swelling induced by dimethylbenzene in mice were observed to compared the differences of their anti-inflammatory effect. And rat acute blood stasis model was used to compare the differences of Leonuri Herba with different processing technology on hemorheology and blood coagulation indexes. Result: Contents of stachydrine hydrochloride, leonurine hydrochloride, rutin, hyperoside and isoquercetin in products of integrated processing were 1. 558%,0. 168%,0. 137%,0. 113% and 0. 078%,they were 1. 482%, 0. 134%, 0. 125%, 0. 082% and 0. 071% in products of traditional processing,respectively. Both of the processing methods could reduce the degree of swelling and the contents of tumor necrosis factor( TNF)-α, interleukin( IL)-1β, IL-6 in mouse serum. And both of the processing methods could reduce the whole blood viscosity,prolong the thrombin time( TT),prothrombin time( PT),activated partial thromboplastin time( APTT) and reduce the concentration of plasma fibrinogen( FIB) in acute blood stasis model rats. Conclusion: Compared with the traditional processing technology,the integrated processing technology is better in guaranteeing the quality of Leonuri Herba decoction pieces and reducing the production cost, which indicates that the integrated processing of Leonuri Herba is reasonable.

引文

[1]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:290-291.
    [2]高学敏.中药学[M].北京:中国中医药出版社,2013:325.
    [3]阮金兰,杜俊荣,曾庆忠,等.益母草的化学、药理和临床研究进展[J].中草药,2003,34(11):15-19.
    [4]宋崎,周小初,宋英,等.益母草炮制工艺的优化[J].时珍国医国药,2009,20(3):709-710.
    [5]王梦溪.益母草药材产地加工与饮片炮制生产一体化工艺研究[D].南京:南京中医药大学,2017.
    [6]单鸣秋,陈星,李娟,等.茜草与茜草炭对大鼠急性血瘀模型的影响比较研究[J].中国中药杂志,2014,39(3):493-497.
    [7]章丽,赵冰洁,袁嘉瑞,等.牡丹皮、赤芍与白芍对急性血瘀模型大鼠活血功效的比较研究[J].中草药,2016,47(15):2676-2683.
    [8]钱岩,单鸣秋,张丽,等.基于化学成分和药理效应分析天麻饮片一体化工艺与传统工艺[J].中国实验方剂学杂志,2016,22(18):5-8.
    [9]张丽,丁安伟.中药材产地加工-饮片炮制一体化研究思路探讨[J].江苏中医药,2016,48(9):70-71.
    [10]秦美蓉,王平,王晓炜,等.盐酸益母草碱和盐酸水苏碱对大鼠离体子宫收缩的影响[J].今日药学,2013,23(7):410-412.
    [11]熊莺,杨解人.益母草碱对大鼠急性心肌缺血损伤心肌肌钙蛋白T的影响[J].中国实验方剂学杂志,2007,13(2):21-24.
    [12]晁志,马丽玲,周秀佳.益母草中生物碱成分对大鼠的利尿作用研究[J].时珍国医国药,2005,16(1):11-12.
    [13]刘林,余纯,徐良.益母草总生物碱抗炎镇痛作用的研究[J].临床医药实践杂志,2008,11(18):934-935.
    [14]李素云,庞惠芳,卫洪昌,等.益母草生物碱和黄酮成分抗大鼠心肌缺血药效学研究[J].山东中医杂志,2006,25(2):114-116.
    [15]张雪,宋玉琴,杨雨婷,等.益母草活血化瘀化学成分与药理作用研究进展[J].药物评价研究,2015,38(2):214-217.
    [16]高冲,刘璐,胡爱菊,等.活血化瘀中药的药理作用研究进展[J].药物评价研究,2013,36(1):64-68.