抗栓通络丸对缺血再灌注损伤大鼠心肌的保护作用
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摘要
目的:探讨抗栓通络丸对缺血再灌注损伤大鼠心肌的保护作用。方法:将40只健康雄性大鼠随机分为模型组、地尔硫卓组、中药高剂量组及中药低剂量组,每组各10只。模型组给予生理盐水10 mL·kg~(-1)灌胃,中药高剂量组、中药低剂量组及地尔硫卓组分别给予抗栓通络丸浓度为0.045 g·L~(-1)、0.180 g·L~(-1)及地尔硫卓5 mg·kg~(-1)加氯化钠溶液配制成药液灌胃,灌胃1周后采用结扎左冠状动脉前降支方法制备大鼠心肌缺血再灌注损伤模型。动态II导联心电图监测缺血再灌注损伤期间各组大鼠心率及ST段变化值;采用酶联免疫吸附法(ELISA)检测血清白细胞介素-6(interleukin-6,IL-6)的含量。结果:与模型组比较,中药高剂量组和地尔硫卓组都可有效减慢缺血再灌注损伤不同时间段的心率,差异具有统计学意义(P<0.05);中药低剂量组与模型组比较,差异无统计学意义(P>0.05)。与模型组比较,中药高剂量组和地尔硫卓组的下降幅度最为明显,缺血20 min起不同时间段比较,差异有统计学意义(P<0.05),而中药低剂量组缺血再灌注损伤各时间点ST段的降低幅度与模型组比较,差异无统计学意义(P>0.05)。与模型组比较,中药高剂量组、中药低剂量组、地尔硫卓组都可降低血清中IL-6含量,中药高剂量组高于中药低剂量组,差异均有统计学意义(P<0.05)。结论:抗栓通络丸可改善缺血再灌注损伤心肌超微结构及异常心电图,降低IL-6含量,抑制炎症反应,从而发挥保护心肌的作用。
To explore the effect of Antithrombosis and Channel-Unblocking Pills on myocardium of rats with ischemia-reperfusion injury.Methods:Forty healthy male rats were randomly divided into the model group,the diltiazem group,the high-dose TCM group and the low-dose TCM group,with 10 rats in each group.The model group was given 10 mL·kg~(-1) of saline by intragastric administration,while the high-dose TCM group,the low-dose TCM group and the diltiazem group were given KSTLP with 0.045 g·L~(-1),0.180 g·L~(-1) and 5 mg·kg~(-1) concentration,and diltiazem of 5 mg·kg~(-1) plus sodium chloride solution respectively.Myocardial ischemia-reperfusion injury model in rats was established by ligating the anterior descending branch of left coronary artery after one week of intragastric administration.The changes of heart rate and ST segment during ischemia-reperfusion injury in each group were monitored by dynamic lead II electrocardiogram,and the levels of interleukin-6(IL-6) in serum were measured by enzyme-linked immunosorbent assay(ELISA).Results:Compared with the model group,the high-dose TCM group and the diltiazem group could effectively slow down the heart rate in different time periods of ischemia-reperfusion injury(P<0.05).There was no significant difference between the low-dose TCM group and the model group(P>0.05).Compared with the model group,the decrease of ST segment in the high-dose TCM group and the diltiazem group was the most obvious.There was significant difference in different time periods of ischemia from the 20~(th) minute(P<0.05),while there was no significant difference in ST segment between the low-dose TCM group and the model group at different time points of ischemia-reperfusion injury(P>0.05).Compared with the model group,the high-dose TCM group,the low-dose TCM group and the diltiazem group could reduce the level of IL-6 in serum caused by ischemia-reperfusion injury.The high-dose TCM group was higher than the low-dose TCMgroup,and the difference was statistically significant(P<0.05).Conclusion:Antithrombosis and Channel-Unblocking Pills can improve myocardial ultrastructure and abnormal electrocardiogram in ischemia-reperfusion injury,reduce the content of IL-6,inhibit inflammation,and play a protective role in myocardium.
To explore the effect of Antithrombosis and Channel-Unblocking Pills on myocardium of rats with ischemia-reperfusion injury.Methods:Forty healthy male rats were randomly divided into the model group,the diltiazem group,the high-dose TCM group and the low-dose TCM group,with 10 rats in each group.The model group was given 10 mL·kg~(-1) of saline by intragastric administration,while the high-dose TCM group,the low-dose TCM group and the diltiazem group were given KSTLP with 0.045 g·L~(-1),0.180 g·L~(-1) and 5 mg·kg~(-1) concentration,and diltiazem of 5 mg·kg~(-1) plus sodium chloride solution respectively.Myocardial ischemia-reperfusion injury model in rats was established by ligating the anterior descending branch of left coronary artery after one week of intragastric administration.The changes of heart rate and ST segment during ischemia-reperfusion injury in each group were monitored by dynamic lead II electrocardiogram,and the levels of interleukin-6(IL-6) in serum were measured by enzyme-linked immunosorbent assay(ELISA).Results:Compared with the model group,the high-dose TCM group and the diltiazem group could effectively slow down the heart rate in different time periods of ischemia-reperfusion injury(P<0.05).There was no significant difference between the low-dose TCM group and the model group(P>0.05).Compared with the model group,the decrease of ST segment in the high-dose TCM group and the diltiazem group was the most obvious.There was significant difference in different time periods of ischemia from the 20~(th) minute(P<0.05),while there was no significant difference in ST segment between the low-dose TCM group and the model group at different time points of ischemia-reperfusion injury(P>0.05).Compared with the model group,the high-dose TCM group,the low-dose TCM group and the diltiazem group could reduce the level of IL-6 in serum caused by ischemia-reperfusion injury.The high-dose TCM group was higher than the low-dose TCMgroup,and the difference was statistically significant(P<0.05).Conclusion:Antithrombosis and Channel-Unblocking Pills can improve myocardial ultrastructure and abnormal electrocardiogram in ischemia-reperfusion injury,reduce the content of IL-6,inhibit inflammation,and play a protective role in myocardium.
引文
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[7] 乐智勇,秦晓林,方念伯,等.泽泻汤对心肌缺血再灌注损伤大鼠血流动力学的影响[J].湖北中医药大学学报,2012,14(5):3-5.
[8] MILNE S E,TROY A,IRW IN M G,et al.Relationship between bispectral index,auditory evoked potential index and effect-site EC50 for propofol at two clinical end points[J].Br JAnaesth,2003,90(2):127-131.
[9] ELMV S A,WABBY M A,EI NAWAWAY M.The effect of anaesthesia and surgery on plasma cytokine production.Anaesthesia,1999,54(8):733-738.
[10] 马育轩,郭蕊珠,周海纯,等.心肌缺血再灌注损伤的中医药治疗研究进展[J].中医药信息,2013,30(5):116-118.
[2] 尚立芝,王建人,崔明霞,等.益母草对大鼠心肌缺血再灌注损伤影响及机制的实验研究[J].河南中医学院学报,2007,22(2):21-23.
[3] 王峰,尚立芝,王建人.川芎嗪预处理对大鼠心肌缺血再灌注损伤作用及其机制研究[J].河南中医,2008,28(4):26-28.
[4] 杜英,赵兰梅,高绍芳.理气活血中药防治心肌缺血的研究概况[J].河南中医,2010,30(12):1247-1249.
[5] 范谦,王跃民,马新亮,等.小鼠心肌缺血-再灌注损伤模型制备方法的改良[J].心脏杂志,2004,16(6):584.
[6] 白玉花,辛颖.心肌缺血再灌注损伤模型建立方法的研究进展[J].内蒙古民族大学学报(自然科学版),2015,30(3):251-256.
[7] 乐智勇,秦晓林,方念伯,等.泽泻汤对心肌缺血再灌注损伤大鼠血流动力学的影响[J].湖北中医药大学学报,2012,14(5):3-5.
[8] MILNE S E,TROY A,IRW IN M G,et al.Relationship between bispectral index,auditory evoked potential index and effect-site EC50 for propofol at two clinical end points[J].Br JAnaesth,2003,90(2):127-131.
[9] ELMV S A,WABBY M A,EI NAWAWAY M.The effect of anaesthesia and surgery on plasma cytokine production.Anaesthesia,1999,54(8):733-738.
[10] 马育轩,郭蕊珠,周海纯,等.心肌缺血再灌注损伤的中医药治疗研究进展[J].中医药信息,2013,30(5):116-118.
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