黄芪注射液联合葛根素注射液对HK-2细胞TGF-β1/Smads及BMP-7/Smad5信号通路的影响

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英文篇名：Effects of Huangqi Injection combined with Gegensu Injection on TGF-β_1/Smads and BMP-7/Smad 5 signaling pathway in HK-2 cells 作者：傅亮 ; 李玉杰 ; 来媛媛 ; 车宇娥 ; 李姝玉 ; 冯婧 ; 吴丹 ; 储著朗 英文作者：FU Liang;LI Yujie;LAI Yuanyuan;School of Preclinical Medicine, Beijing University of Chinese Medicine; 关键词：HK-2细胞 ; 黄芪注射液 ; 葛根素注射液 ; 糖尿病肾病 ; 转化生长因子-β_1 ; 骨形态发生蛋白-7 英文关键词：HK-2 cells;;Huangqi injection;;Gegensu injection;;Diabetic nephropathy;;TGF-β_1;;BMP-7 中文刊名：HQZY 英文刊名：Global Traditional Chinese Medicine 机构：北京中医药大学中医学院; 出版日期：2019-03-06 出版单位：环球中医药 年：2019 期：v.12 基金：国家自然科学基金(81072926);; 北京中医药大学新奥奖励基金(2017-XAJLJJ-003) 语种：中文; 页：HQZY201903005 页数：5 CN：03 ISSN：11-5652/R 分类号：29-33

摘要

目的研究探讨黄芪注射液联合葛根素注射液减缓和治疗肾间质纤维化、保护并改善肾脏功能的相关机制。方法以体外正常培养的人近曲肾小管上皮细胞(HK-2细胞)作为正常组、加入TGF-β_1诱导刺激48小时的为模型组、加入黄芪注射液联合葛根素注射液与TGF-β_1共培养48h的为治疗组,利用Real-time PCR技术分别检测a-平滑肌肌动蛋白(alpha-smooth muscle actin,α-SMA)、E-钙粘连素(E-cadherin)、转化生长因子-β_1及其受体(transforming growth factor-β_1/R, TGF-β_1/R)、转导蛋白3/7(Smad3/7)、骨形态发生蛋白-7及其受体(bone morphogenetic proteins-7/R, BMP-7/R)和转导蛋白5(Smad5)的基因表达。结果与模型组相比,黄芪注射液联合葛根素注射液的治疗组中α-SMA,TGF-β_1/R和Smad3/7的表达明显下降,而E-cadherin,BMP-7/R和Smad5的表达显著升高。结论黄芪注射液联合葛根素注射液可以延缓和治疗肾间质纤维化,并对肾脏起到保护作用,其机制可能与降低肾组织TGF-β_1过度表达、阻断TGF-β_1/Smads信号通路和增强BMP-7的表达、激活BMP-7/Smad5通路有关。
        Objective To investigate the mechanism of Huangqi Injection combined with Gegensu Injection in relieving and treating renal interstitial fibrosis, protecting and improving renal function. Methods Human proximal renal tubular epithelial cells(HK-2 Cells) were cultured in vitro as normal group, while those stimulated by TGF-β_1 for 48 hours as model group, and those co-cultured with Huangqi Injection and Gegensu Injection and TGF-β_1 for 48 hours as treatment group. The gene expressions of alpha-smooth muscle actin(α-SMA), E-cadherin, transforming growth factor-β_1/R(TGF-β_1/R), Smad3/7, bone morphogenetic proteins-7/R(BMP-7/R) and Smad5 were detected by Real-time PCR respectively. Results Compared with the model group, the expressions of α-SMA, TGF-β_1/R and Smad3/7 in the treatment group decreased significantly, while the expressions of E-cadherin, BMP-7/R and Smad5 increased significantly. Conclusion Huangqi Injection combined with Gegensu Injection can delay and treat renal interstitial fibrosis and protect the kidney. The mechanism may be related to reducing the excessive expression of TGF-β_1 in renal tissue, blocking the TGF-β_1/Smads signaling pathway, increasing the expression of BMP-7 and activating the BMP-7/Smad5 pathway.

引文

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