无患子总皂苷对人肝癌细胞Huh7增殖与凋亡的影响
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摘要
为了验证无患子总皂苷对人肝癌细胞Huh7增殖和凋亡的影响,采用不同药物质量浓度的无患子总皂苷对Huh7细胞进行作用,用MTT法和流式细胞术检测作用后细胞的增殖抑制率、细胞周期和凋亡.实验结果表明无患子皂苷对Huh7细胞有明显的抑制作用;显微镜下观察发现无患子总皂苷作用后的细胞有明显的形态学变化;并通过流式细胞仪发现,无患子皂苷作用后的细胞出现明显的凋亡峰,且细胞被阻滞在G0/G1期.综上所述,无患子皂苷可以抑制人肝癌细胞Huh7的增殖并诱导凋亡.
To investigate the effect of sapindus-saponin on the proliferation of Huh7 cell lines.Huh7 cell lines were treated by different doses of sapindus-saponin. Huh7 cell viability were evaluated by MTT assay,cell change in morphology was observed by microscope,Fluorescence flow cytometry( FCM) was applied to detect cell cycle and cell apoptosis. Sapindus-saponin could inhibit the proliferation of Huh7 cell line in a dose and time dependent manner. Morphological changes of cells were observed. FCM assay indicated that apoptosis peak appeared evidently and Huh7 cells were atrested in G0/G1 phase. Sapindus-saponin could inhibit the proliferation of Huh7 cell line and induce their apoptosis.
To investigate the effect of sapindus-saponin on the proliferation of Huh7 cell lines.Huh7 cell lines were treated by different doses of sapindus-saponin. Huh7 cell viability were evaluated by MTT assay,cell change in morphology was observed by microscope,Fluorescence flow cytometry( FCM) was applied to detect cell cycle and cell apoptosis. Sapindus-saponin could inhibit the proliferation of Huh7 cell line in a dose and time dependent manner. Morphological changes of cells were observed. FCM assay indicated that apoptosis peak appeared evidently and Huh7 cells were atrested in G0/G1 phase. Sapindus-saponin could inhibit the proliferation of Huh7 cell line and induce their apoptosis.
引文
[1]刘会春.中国原发性肝癌治疗指南解读[J].肝胆外科杂志,2013,21(1):12-14.
[2]樊嘉,王征.原发性肝癌综合治疗新进展[J].国际消化病杂志,2013,33(2):73-74.
[3]戴朝六,赵阳.原发性肝癌的综合治疗[J].中国普外基础与临床杂志,2014(2):133-137.
[4]黄素梅,王敬文,杜孟浩,等.无患子的研究现状及其开发利用[J].林业科技开发,2009,23(6):1-5.
[5]郭英,谢建平,柳爱华,等.无患子药理作用概述[J].中国病原生物学杂志,2011(11):873-874.
[6] CHEN C Y,KUO P L,CHEN Y H,et al. Tyrosinase inhibition,free radical scavenging,antimicroorganism and anti-cancer proliferation activities of Sapindus mukorossi extracts[J]. Journal of the Taiwan Institute of Chemical Engineers,2010,41(2):129-135.
[7]长尾常敦.关于肿瘤细胞增殖抑制成分的研究(14),无患子果皮中的活性成分[J].国外医学:中医中药分册,2002,24(4):246-247.
[8] HUANG H C,WU M D,TSAI W J,et al. Triterpenoid saponins from the fruits and galls of Sapindus mukorossi[J].Phytochemistry,2008,69(7):1609-1616.
[9] BAI Y,QU X Y,YIN J Q,et al. Methyl protodioscin induces G2/M cell cycle arrest and apoptosis in A549 humanlung cancer cells[J]. Pharmacognosy Magazine,2014,10(39):318.
[10] FUJIOKA T,KASHIWADA Y,OKABE H,et al. Antitumor agents 171. Cytotoxicities of lobatosides B,C,D,andE,cyclic bisdesmosides isolated from Actinostemma lobatum maxim[J]. Bioorganic&Medicinal Chemistry Letters,1996,6(23):2807-2810.
[11]盛辉,罗良,明月,等.大豆皂甙对人肺腺癌细胞增殖抑制作用研究[J].中国老年学,2003,23(12):809-810.
[12]陈声武,王岩,王毅,等.人参皂苷Rg-1和Rh-1抗肿瘤作用的研究[J].吉林大学学报(医学版),2003,29(1):25-28.
[13] SATO K,MOCHIZUKI M,SAIKI I,et al. Inhibition of tumor angiogenesis and metastasis by a saponin of Panax gin-seng,ginsenoside-Rb2[J]. Biological&Pharmaceutical Bulletin,1994,17(5):635-639.
[2]樊嘉,王征.原发性肝癌综合治疗新进展[J].国际消化病杂志,2013,33(2):73-74.
[3]戴朝六,赵阳.原发性肝癌的综合治疗[J].中国普外基础与临床杂志,2014(2):133-137.
[4]黄素梅,王敬文,杜孟浩,等.无患子的研究现状及其开发利用[J].林业科技开发,2009,23(6):1-5.
[5]郭英,谢建平,柳爱华,等.无患子药理作用概述[J].中国病原生物学杂志,2011(11):873-874.
[6] CHEN C Y,KUO P L,CHEN Y H,et al. Tyrosinase inhibition,free radical scavenging,antimicroorganism and anti-cancer proliferation activities of Sapindus mukorossi extracts[J]. Journal of the Taiwan Institute of Chemical Engineers,2010,41(2):129-135.
[7]长尾常敦.关于肿瘤细胞增殖抑制成分的研究(14),无患子果皮中的活性成分[J].国外医学:中医中药分册,2002,24(4):246-247.
[8] HUANG H C,WU M D,TSAI W J,et al. Triterpenoid saponins from the fruits and galls of Sapindus mukorossi[J].Phytochemistry,2008,69(7):1609-1616.
[9] BAI Y,QU X Y,YIN J Q,et al. Methyl protodioscin induces G2/M cell cycle arrest and apoptosis in A549 humanlung cancer cells[J]. Pharmacognosy Magazine,2014,10(39):318.
[10] FUJIOKA T,KASHIWADA Y,OKABE H,et al. Antitumor agents 171. Cytotoxicities of lobatosides B,C,D,andE,cyclic bisdesmosides isolated from Actinostemma lobatum maxim[J]. Bioorganic&Medicinal Chemistry Letters,1996,6(23):2807-2810.
[11]盛辉,罗良,明月,等.大豆皂甙对人肺腺癌细胞增殖抑制作用研究[J].中国老年学,2003,23(12):809-810.
[12]陈声武,王岩,王毅,等.人参皂苷Rg-1和Rh-1抗肿瘤作用的研究[J].吉林大学学报(医学版),2003,29(1):25-28.
[13] SATO K,MOCHIZUKI M,SAIKI I,et al. Inhibition of tumor angiogenesis and metastasis by a saponin of Panax gin-seng,ginsenoside-Rb2[J]. Biological&Pharmaceutical Bulletin,1994,17(5):635-639.
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