胃癌患者手术前后miRNA-101、miRNA-21表达水平及其临床意义
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摘要
目的探讨胃癌患者手术前后血浆miRNA-101、miRNA-21表达水平及其临床意义。方法选取50例行胃癌根治术的胃癌患者为胃癌组,另选取同期健康体检者50例为健康对照组,分别于术前、术后1周及术后1个月检测胃癌患者,体检时检测健康体检者血浆中miRNA-101、miRNA-21表达水平;并比较不同临床特征胃癌患者的血浆miRNA-101、miRNA-21表达水平。结果术前胃癌组患者血浆miRNA-101相对表达量明显低于健康对照组,miRNA-21相对表达量明显高于健康对照组(P﹤0.01)。与术前比较,术后1周及术后1个月胃癌患者血浆miRNA-101相对表达量逐渐增高,血浆miRNA-21相对表达量逐渐降低(P﹤0.05)。不同分化程度、TNM分期、淋巴结转移情况的胃癌患者术前血浆miRNA-101、miRNA-21相对表达量比较,差异均有统计学意义(P﹤0.05)。结论胃癌患者血浆miRNA-101、miRNA-21可作为胃癌潜在的生物标志物,有望成为胃癌诊断、手术疗效评估的新手段。
Objective To investigate the expression of plasma miRNA-101 and miRNA-21 in patients with gastric cancer before and after operation and their clinical significance. Method The research contained a cohort including 50 patients with gastric cancer as gastric cancer group, and another 50 healthy examined adults who were included as healthy control group. The expression of miRNA-101 and miRNA-21 in plasma of patients with gastric cancer were detected before surgery, at 1 week and 1 month after operation, healthy examined adults were detected during physical examination,respectively. Meanwhile, the expression of plasma miRNA-101 and miRNA-21 were compared between gastric cancer patients with different clinical characteristics. Result The relative expression level of plasma miRNA-101 was significantly lower while that of miRNA-21 was significantly higher in gastric cancer group than in healthy control group before surgery(P<0.01). While miRNA-101 increased significantly and miRNA-21 decreased significantly in gastric cancer group at 1 week and 1 month after operation, compared with those before surgery(P<0.05). The relative expression levels of plasma miRNA-101 and miRNA-21 in gastric cancer patients with different degrees of differentiation, TNM stages and lymph node metastasis before surgery were compared, and the differences were statistically significant(P<0.05). CCoonncclluu-sion miRNA-101 and miRNA-21 in plasma of patients with gastric cancer can be used as potential biomarkers for gastric cancer, which is expected to become a new way for diagnosis of gastric cancer and evaluation of the surgical effects.
Objective To investigate the expression of plasma miRNA-101 and miRNA-21 in patients with gastric cancer before and after operation and their clinical significance. Method The research contained a cohort including 50 patients with gastric cancer as gastric cancer group, and another 50 healthy examined adults who were included as healthy control group. The expression of miRNA-101 and miRNA-21 in plasma of patients with gastric cancer were detected before surgery, at 1 week and 1 month after operation, healthy examined adults were detected during physical examination,respectively. Meanwhile, the expression of plasma miRNA-101 and miRNA-21 were compared between gastric cancer patients with different clinical characteristics. Result The relative expression level of plasma miRNA-101 was significantly lower while that of miRNA-21 was significantly higher in gastric cancer group than in healthy control group before surgery(P<0.01). While miRNA-101 increased significantly and miRNA-21 decreased significantly in gastric cancer group at 1 week and 1 month after operation, compared with those before surgery(P<0.05). The relative expression levels of plasma miRNA-101 and miRNA-21 in gastric cancer patients with different degrees of differentiation, TNM stages and lymph node metastasis before surgery were compared, and the differences were statistically significant(P<0.05). CCoonncclluu-sion miRNA-101 and miRNA-21 in plasma of patients with gastric cancer can be used as potential biomarkers for gastric cancer, which is expected to become a new way for diagnosis of gastric cancer and evaluation of the surgical effects.
引文
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[13]周飞梅,马晓欣. miR-101在子宫内膜癌组织中的表达及其与临床病理参数的关系[J].现代肿瘤医学, 2017, 25(3):436-440.
[14]严晓丽,杜梦楠,郑纪宁.大肠癌石蜡包埋组织中MiR-21表达变化及意义[J].山东医药, 2017, 57(10):11-14.
[15]刘洁,彭微,吴稚晖,等.结肠癌患者手术前后血浆miR-21表达水平变化及其临床意义[J].肿瘤防治研究, 2015,42(8):794-796.
[16]蒋志强,李亚兰,韩广森,等.微小RNA-101在结肠癌组织的表达及其与患者预后的关系[J].中华实验外科杂志, 2016, 33(5):1354-1356.
[2]田晓琳,杨臻,王建英,等.微小RNA与肿瘤的关系[J].癌症进展, 2016, 14(1):22-25.
[3] Zhang J, Liu J, Liu Y, et al. miR-101 represses lung cancer by inhibiting interaction of fibroblasts and cancer cells by down-regulating CXCL12[J]. Biomed Pharmacother, 2015,74:215-221.
[4]黄声凯,王佳,李燕,等.血清miR-378和miR-21联合检测对胃癌的诊断价值[J].中华肿瘤杂志, 2018, 40(6):441-445.
[5]顾荣民,朱明,文旭,等.胃癌手术前后血循环miR-21表达与胃癌临床病理特征的关系研究[J].国际检验医学杂志,2014, 35(24):3316-3318.
[6] Puhan MA, Yu T, Stegeman I, et al. Benefit-harm analysis and charts for individualized and preference-sensitive prevention:example of low dose aspirin for primary prevention of cardiovascular disease and cancer[J]. BMC Med,2015, 13:250.
[7]赖明广,青海涛,王立生. microRNA-101和COX-2在结肠癌中的表达及其临床意义[J].实用医学杂志, 2016, 32(5):739-742.
[8]段旭艳. miR-21、FOXO3a、STAT3表达与胃癌及临床病理参数的关系[J].海南医学, 2018, 29(3):320-323.
[9]谭亭昭,吴超,王媛,等. miR-21表达与胃癌细胞阿帕替尼敏感性关系的实验研究[J].临床肿瘤学杂志, 2018, 23(7):598-603.
[10]许新才,林海,张旺,等.胃癌组织中miRNA-21和miRNA-34的表达及其意义[J].新疆医科大学学报, 2016, 39(7):854-857.
[11]宋雅琴.血清miR-101与miR-25在胃癌发生发展过程中的作用及其诊断价值[D].广州:暨南大学, 2016.
[12] Wang B, Zhang Q. The expression and clinical significance of circulating microRNA-21 in serum of five solid tumors[J]. J Cancer Res Clin Oncol, 2012, 138(10):1659-1666.
[13]周飞梅,马晓欣. miR-101在子宫内膜癌组织中的表达及其与临床病理参数的关系[J].现代肿瘤医学, 2017, 25(3):436-440.
[14]严晓丽,杜梦楠,郑纪宁.大肠癌石蜡包埋组织中MiR-21表达变化及意义[J].山东医药, 2017, 57(10):11-14.
[15]刘洁,彭微,吴稚晖,等.结肠癌患者手术前后血浆miR-21表达水平变化及其临床意义[J].肿瘤防治研究, 2015,42(8):794-796.
[16]蒋志强,李亚兰,韩广森,等.微小RNA-101在结肠癌组织的表达及其与患者预后的关系[J].中华实验外科杂志, 2016, 33(5):1354-1356.
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