响应面法优化月季叶总黄酮提取工艺的研究
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摘要
优化月季叶总黄酮提取工艺,为其技术开发提供参考。以月季叶为研究对象,采用单因素试验分别考察提取次数、剂料比、提取温度、乙醇体积分数和提取时间等因素对月季叶总黄酮提取得率的影响。在此基础上,以总黄酮提取得率为指标,采用Central Composite Design(CCD)中心组合设计试验,响应面法分析结果,建立数学模型优化月季叶总黄酮提取工艺。结果表明,各因素对月季叶总黄酮提取得率的影响大小依次为:提取温度>提取时间>剂料比>乙醇体积分数;优化的提取条件为:提取温度66℃,提取时间1.8 h,剂料比23∶1,乙醇体积分数61%,此条件下,一次提取的月季叶总黄酮的提取得率为12.84 mg/g。分别对比6组模型预测值与试验值,发现二者偏差均在5%以内,说明建立的数学模型较为可靠,可用于对月季叶总黄酮提取工艺的优化。
The extraction process of total flavonoids from Rosa chinensis leaves was optimized in order to provide technical references for the development and utilization of Rosa chinensis leaves. The effect of extraction times,liquid-solid ratio,extraction temperature,ethanol concentration and extraction time on the yield of total flavonoids from Rosa chinensis leaves were investigated using single-factor test. Based on the single-factor experiment results,response surface analysis methodology was employed to optimize the extraction process of total flavonoids. A mathematical model was established to evaluate factors influencing on extraction yield by central composite design(CCD). The results showed that the factors affecting yield of total flavonoids were in the order of :extraction temperature,extraction time,liquid-solid ratio and ethanol concentration. The optimal conditions for total flavonoids extraction were as follows:extraction temperature 66 ℃,extraction time 1.8 h,liquid-solid ratio 23 ∶1 and ethanol concentration 61%,and the yield of total flavonoids was up to 12.84 mg/g under these conditions. Comparing six groups of experimental results with the predicted results of mathematical models found that the deviation between the two results was less than 5%,indicating that the established mathematical model was reliable and could be used to optimize the extraction process of total flavonoids from Rosa chinensis leaves.
The extraction process of total flavonoids from Rosa chinensis leaves was optimized in order to provide technical references for the development and utilization of Rosa chinensis leaves. The effect of extraction times,liquid-solid ratio,extraction temperature,ethanol concentration and extraction time on the yield of total flavonoids from Rosa chinensis leaves were investigated using single-factor test. Based on the single-factor experiment results,response surface analysis methodology was employed to optimize the extraction process of total flavonoids. A mathematical model was established to evaluate factors influencing on extraction yield by central composite design(CCD). The results showed that the factors affecting yield of total flavonoids were in the order of :extraction temperature,extraction time,liquid-solid ratio and ethanol concentration. The optimal conditions for total flavonoids extraction were as follows:extraction temperature 66 ℃,extraction time 1.8 h,liquid-solid ratio 23 ∶1 and ethanol concentration 61%,and the yield of total flavonoids was up to 12.84 mg/g under these conditions. Comparing six groups of experimental results with the predicted results of mathematical models found that the deviation between the two results was less than 5%,indicating that the established mathematical model was reliable and could be used to optimize the extraction process of total flavonoids from Rosa chinensis leaves.
引文
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[12]黎晨晨,张煜,迟逍遥,等.响应面法优化花椰菜总黄酮提取工艺的研究[J].河南工业大学学报(自然科学版),2018,39(2):87-92.
[2] ZHENG Y Z,DENG G,CHEN D F,et al. The influence of C2=C3 double bond on the antiradical activity of flavonoid:different mechanisms analysis[J]. Phytochemistry,2019,157:1-7.
[3] LIN K H,SHIBU M A,PERAMAIYAN R,et al.Bioactive flavone fisetin attenuates hypertension associated cardiac hypertrophy in H9c2 cells and in spontaneously hypertension rats[J].Journal of Functional Foods,2019,52:212-218.
[4] ZAKARYAN H,ARABYAN E,OO A,et al. Flavonoids:promising natural compounds against viral infections[J]. Archives of Virology,2017,162(9):2539-2551.
[5] WANG T,LI Q,BI K. Bioactive flavonoids in medicinal plants:structure,activity and biological fate[J]. Asian Journal of Pharmaceutical Sciences,2018,13(1):12-23.
[6] JNAWALI H N,JEON D,JEONG M C,et al. Antituberculosis activity of a naturally occurring flavonoid,isorhamnetin[J]. Journal of Natural Products,2016,79(4):961-969.
[7]翁竞玉,陈俊,刘坤玲,等.天然黄酮类化合物防治糖尿病肾病的研究进展[J].药学研究,2018,37(10):593-596.
[8] ZHANG X,LI H L,ZHANG H R,et al. Inhibition of transmembrane member 16A calcium activated chloride channels by natural flavonoids contributes to flavonoid anticancer effects[J]. British Journal of Pharmacology,2017,174:2334-2345.
[9]廉慧杰,田静,木卡旦斯·依明,等.银杏叶总黄酮提取分离技术研究进展[J].现代园艺,2018(18):13-14.
[10]陈建福,胡泽杰,潘诗玲,等.超声波辅助双水相提取胡萝卜叶总黄酮的工艺研究[J].河南工业大学学报(自然科学版),2018,39(1):72-77.
[11]许远,魏和平,吴彦,等.响应面优化襄荷总黄酮提取及抗氧化研究[J].食品工业科技,2015,36(5):233-239.
[12]黎晨晨,张煜,迟逍遥,等.响应面法优化花椰菜总黄酮提取工艺的研究[J].河南工业大学学报(自然科学版),2018,39(2):87-92.