卵磷脂桶混助剂的特性及其在防治稻飞虱中对氟啶虫胺腈的协同增效作用
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摘要
为明确新型卵磷脂类桶混助剂"融透"的特性,采用透射电镜和激光粒度分析仪表征了其物理性质,并通过室内生物测定和田间药效试验测定了其在防治稻飞虱中对氟啶虫胺腈的协同增效作用。结果表明,"融透"中含有圆形脂质体,Z平均粒径为129.5 nm。室内生物测定结果表明,"融透"可使氟啶虫胺腈对褐飞虱的毒力增加1.67倍。田间药效试验表明,"融透"可使氟啶虫胺腈对稻飞虱的防效提高9.04%～41.77%;当氟啶虫胺腈减量40%时,添加"融透"的处理对稻飞虱的防效与未添加"融透"的常量组相当。研究结果表明,卵磷脂桶混助剂"融透"可以通过形成脂质体提高药剂的利用率,从而达到增效的作用。
In order to investigate the property of "Transmicelle" a new lecithin tank mixing adjuvant, its physical characteristics were investigated by transmission electron microscopy(TEM) and laser particle size analyzer, and its synergistic effect in controlling rice planthopper was determined by bioassay and field trials. The results showed that "Transmicelle" contained round liposomes with a Z-average size of129.5 nm. The bioassay exhibited that "Transmicelle" can increase the toxicity of sulfoxaflor by 1.67 times. Furthermore, the field trial results indicated that the control effect of sulfoxaflor was increased by9.04%-41.77% with the "Transmicelle". Compared to the normal dosage treatment without"Transmicelle", no significant difference in the control effect was detected by adding "Transmicelle" if the amont sulfoxaflor was reduced by 40%. The above results illustrate that the "Transmicelle" of the lecithin tank mixing adjuvant can enhance the utilization rate of the pesticide by forming liposomes,which results in a synergistic effect.
In order to investigate the property of "Transmicelle" a new lecithin tank mixing adjuvant, its physical characteristics were investigated by transmission electron microscopy(TEM) and laser particle size analyzer, and its synergistic effect in controlling rice planthopper was determined by bioassay and field trials. The results showed that "Transmicelle" contained round liposomes with a Z-average size of129.5 nm. The bioassay exhibited that "Transmicelle" can increase the toxicity of sulfoxaflor by 1.67 times. Furthermore, the field trial results indicated that the control effect of sulfoxaflor was increased by9.04%-41.77% with the "Transmicelle". Compared to the normal dosage treatment without"Transmicelle", no significant difference in the control effect was detected by adding "Transmicelle" if the amont sulfoxaflor was reduced by 40%. The above results illustrate that the "Transmicelle" of the lecithin tank mixing adjuvant can enhance the utilization rate of the pesticide by forming liposomes,which results in a synergistic effect.
引文
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[22]封云涛,李光玉,郭晓君,等.两种表面活性助剂在农药减量化防治小菜蛾中的应用[J].农药学学报,2015,17(5):603-609.FENG Y T,LI G Y,GUO X J,et al.Study on application of two kinds of surfactant in dose-reduced chemical control of Plutella xylostella(Linnaeus)[J].Chin J Pestic Sci,2015,17(5):603-609.
[23]VANCE D E,VANCE J E.Preface[M]//VANCE D E,VANCE J E.eds.Biochemistry of lipids,lipoproteins and membranes.Elsevier,2008:v-vi.
[24]GREGORIADIS G.Liposome technology:Entrapment of drugs and other materials into liposomes[M/OL].3rd ed.Boca Raton:CRCPress,2006:4-19.https://www.crcpress.com/Liposome-TechnologyEntrapment-of-Drugs-and-Other-Materials-into-Liposomes/Gregoriadis/p/book/9780849388286?source=crcpress.com&utmsourc e=productpage&utmmedium=website&utmcampaign=RelatedTitles.
[25]PATRICK G L.An introduction to medicinal chemistry[M].6th ed.Oxford:Oxford University Press,2017:4-20.
[26]SANDEEP K.Interaction of liposomes with cancer cells:Influence of liposome surface properties on cellular uptake[J].Int J Pharm Bio Sci,2016,7(1):199-205.
[27]TOMLIN C D S.The pesticide manual,a world compendium[M/OL].15th ed.Alton,Hampshire:BCPC,2009:3-10.https://www.bcpc.org/product/the-pesticide-manual-18th-edition.
[2]张忠亮,李相全,王欢,等.六种有机硅助剂对氟磺胺草醚的增效作用及其增效机理初探[J].农药学学报,2015,17(1):115-118.ZHANG Z L,LI X Q,WANG H,et al.Preliminary studies on synergism and mechanisms of six organosilicon additives on fomesafen[J].Chin J Pestic Sci,2015,17(1):115-118.
[3]MELO A A,USANO-ALEMANY J,GUEDES J V C,et al.Impact of tank-mix adjuvants on deposit formation,cuticular penetration and rain-induced removal of chlorantraniliprole[J].Crop Prot,2015,78:253-262.
[4]张萍,姜兴印,谭海丽,等.四种喷雾助剂对嘧菌酯在玉米叶片上耐雨水冲刷能力及其对玉米安全性的影响[J].农药学学报,2018,20(2):239-248.ZHANG P,JIANG X Y,TAN H L,et al.Effects of four spray adjuvants on the rainfastness of azoxystrobin on maize leaf surfaces and their effect on maize safety[J].Chin J Pestic Sci,2018,20(2):239-248.
[5]翟中和,王喜忠,丁明孝.细胞生物学[M].4版.北京:高等教育出版社,2011:6-1.ZHAI Z H,WANG X Z,DING M X.Cell biology[M].4th ed.Beijing:Higher Education Press,2011:6-1.
[6]袁博,周张涛,郑竟成,等.大豆卵磷脂及其复配对DHA藻油氧化稳定性的影响[J].中国食品添加剂,2018(8):152-156.YUAN B,ZHOU Z T,ZHENG J C,et al.Effect of soybean lecithin and multiple antioxidants on oxidation stability of DHA algal oil[J].China Food Additives,2018(8):152-156.
[7]祝浩成,栾立标.脂质体天然高分子水凝胶给药系统的研究进展[J].药学进展,2018,42(5):380-387.ZHU H C,LUAN L B.Research progress in liposome-in-natural polymer hydrogel drug delivery systems[J].Progress in Pharmaceutical Sciences,2018,42(5):380-387.
[8]李国辉,钟其顶,王道兵,等.HPLC法测定大豆磷脂及其保健品中PC、PI、PE[J].食品研究与开发,2015,36(6):67-70.LI G H,ZHONG Q D,WANG D B,et al.Determination of the concentrations of phosphatidylcholine,phosphatidyl ethanolamine and phosphatidylinositol in soybean phospholipids and health care products by HPLC[J].Food Res Develop,2015,36(6):67-70.
[9]高燕.抗肿瘤药多柔比星新型递送系统的研究进展[J].药物生物技术,2018,25(3):273-276.GAO Y.Research progress of new delivery system for antitumor drug doxorubicin[J].Pharmaceutical Biotechnology,2018,25(3):273-276.
[10]贺军波,史浩,张维农,等.基于脂质纳米载体农药包埋体系的研究进展[J].农药,2017,56(10):703-707.HE J B,SHI H,ZHANG W N,et al.Research progress of pesticide encapsulation based on lipid nano-carriers[J].Agrochemicals,2017,56(10):703-707.
[11]张珺.莫西沙星脂质体凝胶肺部靶向介入给药缓释系统的研究[D].哈尔滨:黑龙江中医药大学,2014.ZHANG J.Study on moxifloxacin liposome gel for lung targeting drug delivery system[D].Harbin:Heilongjiang University of Chinese Medicine,2014.
[12]郝艳丽.盐酸拓扑替康普通、长循环及温度敏感脂质体的研究[D].沈阳:沈阳药科大学,2005.HAO Y L.Studies on conventional,long-circulating and temperaturesensitive liposomes containing topotecan hydrochloride[D].Shenyang:Shenyang Pharmaceutical University,2005.
[13]吕佳书,郭欣,姜思亮,等.透明质酸脂质体制备及释放研究[J].黑龙江医药科学,2018,41(3):47-48.LU J S,GUO X,JIANG S L,et al.Study on formulation and release of hyaluronic acid liposomes[J].Heilongjiang Med Pharm,2018,41(3):47-48.
[14]厦门WTO工作站.欧洲允许卵磷脂作为食品添加剂使用[J].食品与生物技术学报,2017,36(5):546.Xiamen WTO Workstation.Europe allow phosphatidylcholine using as a food adjuvant[J].J Food Sci Biotechnol,2017,36(5):546.
[15]BABCOCK J M,GERWICK C B,HUANG J X,et al.Biological characterization of sulfoxaflor,a novel insecticide[J].Pest Manag Sci,2011,67(3):328-334.
[16]顾林玲.美国拟登记陶氏益农氟啶虫胺腈(sulfoxaflor)[J].现代农药,2013,12(2):43.GU L L.The United States intends to register sulfoxaflor from Dow AgroSciences[J].Mod Agrochem,2013,12(2):43.
[17]SPARKS T C,WATSON G B,LOSO M R,et al.Sulfoxaflor and the sulfoximine insecticides:chemistry,mode of action and basis for efficacy on resistant insects[J].Pestic Biochem Physiol,2013,107(1):1-7.
[18]LIAO X,MAO K K,ALI E,et al.Temporal variability and resistance correlation of sulfoxaflor susceptibility among Chinese populations of the brown planthopper Nilaparvata lugens(St?l)[J].Crop Protection,2017,102:141-146.
[19]ZHANG X L,LIAO X,MAO K K,et al.The role of detoxifying enzymes in field-evolved resistance to nitenpyram in the brown planthopper Nilaparvata lugens in China[J].Crop Protection,2017,94:106-114.
[20]ROBERTSON J L,RUSSELL R M,PREISLER H K,et al.Bioassays with Arthropods[M].2nd ed.Boca Raton:CRC Press,2007:6-14.
[21]常菊花,何月平.应用Polo软件进行农药毒力数据的比较分析[J].浙江农业学报,2014,26(6):1552-1557.CHANG J H,HE Y P.The analysis for comparing the pesticide toxicity data using the Polo software[J].Acta Agriculturae Zhejiangensis,2014,26(6):1552-1557.
[22]封云涛,李光玉,郭晓君,等.两种表面活性助剂在农药减量化防治小菜蛾中的应用[J].农药学学报,2015,17(5):603-609.FENG Y T,LI G Y,GUO X J,et al.Study on application of two kinds of surfactant in dose-reduced chemical control of Plutella xylostella(Linnaeus)[J].Chin J Pestic Sci,2015,17(5):603-609.
[23]VANCE D E,VANCE J E.Preface[M]//VANCE D E,VANCE J E.eds.Biochemistry of lipids,lipoproteins and membranes.Elsevier,2008:v-vi.
[24]GREGORIADIS G.Liposome technology:Entrapment of drugs and other materials into liposomes[M/OL].3rd ed.Boca Raton:CRCPress,2006:4-19.https://www.crcpress.com/Liposome-TechnologyEntrapment-of-Drugs-and-Other-Materials-into-Liposomes/Gregoriadis/p/book/9780849388286?source=crcpress.com&utmsourc e=productpage&utmmedium=website&utmcampaign=RelatedTitles.
[25]PATRICK G L.An introduction to medicinal chemistry[M].6th ed.Oxford:Oxford University Press,2017:4-20.
[26]SANDEEP K.Interaction of liposomes with cancer cells:Influence of liposome surface properties on cellular uptake[J].Int J Pharm Bio Sci,2016,7(1):199-205.
[27]TOMLIN C D S.The pesticide manual,a world compendium[M/OL].15th ed.Alton,Hampshire:BCPC,2009:3-10.https://www.bcpc.org/product/the-pesticide-manual-18th-edition.
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