NaOH协同辐照预处理提高稻草纤维素酶解转化率
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摘要
为降低预处理成本,提升稻草纤维素的酶解效果,采用NaOH协同~(60)Co-γ射线辐照处理稻草,研究其对稻草中纤维素酶解转化率的影响,利用扫描电镜(SEM)、红外光谱(FT-IR)分析协同预处理对稻草微观结构的影响,并通过正交试验对协同预处理条件参数进行优化。结果表明,NaOH协同辐照预处理能显著提高稻草纤维素酶解转化率,优化后的预处理条件为400 kGy辐照剂量的稻草结合2%NaOH溶液,固液比1∶15,50℃条件下处理4 h,按照10 FPU·g~(-1)加入纤维素酶溶液,于50℃、130 r·min~(-1)条件下气浴恒温振荡,酶解24 h后,其稻草纤维素转化率达到78.54%±1.20%。本研究结果为农业废弃物资源能源化高效利用提供了技术支撑和理论依据。
To reduced the pretreatment costs, and improved the enzymolysis effect of cellulose in rice straw, the rice straw pretreated by using NaOH solution collaborated with irradiation by ~(60)Coγ-rays were used for studying the effect of enzymatic hydrolysis conversion rate of cellulose in rice straw. Scanning electron microscope(SEM) and infrared spectroscopy(FT-IR) were used for scaning the microstructure of the rice straw that pretreated by above method. The results showed that the enzymatic hydrolysis conversion rate of cellulose in rice straw were significantly improved by the pretreatment. Orthogonal test showed that the rice straw under the pretreatment conditions of 400 kGy irradiation dose and 2% NaOH solution in 50℃ for 4 h with the solid-to-liquid ratio of 1∶15, and the enzymatic hydrolysis conversion rate of cellulose in treated rice straw oscillated under Gas bath constant temperature with 130 r·min~(-1) in 50℃ for 24 h after added cellulase with 10 FPU·g~(-1) was 78.54%±1.20%,This result provided the theoretical basis and technical support for utilizing the agricultural waste biomass resources.
To reduced the pretreatment costs, and improved the enzymolysis effect of cellulose in rice straw, the rice straw pretreated by using NaOH solution collaborated with irradiation by ~(60)Coγ-rays were used for studying the effect of enzymatic hydrolysis conversion rate of cellulose in rice straw. Scanning electron microscope(SEM) and infrared spectroscopy(FT-IR) were used for scaning the microstructure of the rice straw that pretreated by above method. The results showed that the enzymatic hydrolysis conversion rate of cellulose in rice straw were significantly improved by the pretreatment. Orthogonal test showed that the rice straw under the pretreatment conditions of 400 kGy irradiation dose and 2% NaOH solution in 50℃ for 4 h with the solid-to-liquid ratio of 1∶15, and the enzymatic hydrolysis conversion rate of cellulose in treated rice straw oscillated under Gas bath constant temperature with 130 r·min~(-1) in 50℃ for 24 h after added cellulase with 10 FPU·g~(-1) was 78.54%±1.20%,This result provided the theoretical basis and technical support for utilizing the agricultural waste biomass resources.
引文
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[13] Wu L, Arakane, M, Ike M, Wada M, Takai T, Gau M, Tokuyasu K. Low temperature alkali pretreatment for improving enzymatic digestibility of sweet sorghum bagasse for ethanol production[J].Bioresourse Technology,2011,102(7):4793-4799
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[16] 唐洪涛,王锋,李伟明,李安,李彦杰,哈益明.γ射线辐照与NaOH溶液协同预处理对玉米秸秆酶解产糖率及微观结构的影响[J].核农学报,2012,26(3):535-542
[17] 马玉龙,王凤芝,常璇.响应面法优化碱解聚玉米秸秆条件的研究[J].中国粮油学报,2016,31(4):128-132
[18] 武小芬,陈亮,陈静萍,苏小军,齐慧,王克勤,邓明.γ射线辐照降解木聚糖的机理研究[J].核农学报,2017,31(5):889-898
[19] 白献晓,王二柱,朱群,常娟,尹清强,刘超齐,卢富山.碱和蒸汽高压对小麦秸秆中粗纤维降解率的影响[J].河南农业科学,2013,45(11):135-138
[20] Bak J S, Ko J K, Han Y H, Lee B C, Choi Ⅰ G, Kim K H. Improved enzymatic hydrolysis yield of rice straw using electron beam irradiation pretreatment[J].Bioresource Technology,2009,100(3):1285-1290
[21] 沈志强,杨春平,喻国策,王建龙.小麦秸秆的γ辐照与NaOH溶液的协同预处理[J].原子能科学技术,2009,43(4):304-310
[22] 徐红.蒸汽爆破预处理红柳的酶解发酵性能研究[D].南京:南京林业大学,2013:21-22
[23] 马强,顿宝庆,奚亚军,王智,陈朝儒,路明,李桂英.碱处理对甜高粱秸秆渣组成结构及酶解糖化效果的影响[J].作物学报,2014,40(9):1604-1611
[24] 朱圆圆,顾夕梅,朱均均,徐勇,勇强,余世袁.两步碱法预处理对玉米秸秆组分及结构的影响[J].中国科技论文,2015,10(12):1376-1381
[25] Oh S Y, Yoo D Ⅰ, Shin Y, Kim H C, Kim H Y, Chung Y S, Park W H, Youk J H. Crystalline structure analysis of cellulose treated with sodium hydroxide and carbon dioxide by means of X-ray diffraction and FTIR spectroscopy[J]. Carbohydrate Research,2005,340(10):2376-2391
[26] 张荣.基于光谱分析的木材树种识别方法的研究[D]. 南京:南京林业大学,2011:7-8
[27] 范慧青,王喜明,王雅梅.利用傅里叶变换红外光谱法快速测定木材纤维素含量[J].木材加工机械,2014(4):33-37
[28] Peng P, Peng F, Bian J, Xu F, Sun R C, Kennedy J F. Isolation and structural characterization of hemicelluloses from the bamboo species Phyllostachys incarnata Wen[J].Carbohydrate Polymers, 2011,86(2):883-890
[29] 薛晓明,南程慧,吴显坤,陈云霞.三种檀香木的红外光谱比较研究[J].湖北农业科学,2014,53(17):4096-4099
[30] 肖霄,李居南,王志文, 於祥,徐晖.龙竹半纤维素的提取分离及结构表征[J].西北农业学报,2014,23(5):195-201
[31] 鲁南,沈锋,王胜丹,杨桂花,陈嘉川.巨菌草的木素及半纤维素分析[J].中国造纸,2016,35(8):1-6
[32] 王继坤,赵力超,刘欣,曹庸,曹素芳.广林9号桉叶木质素的结构表征[J].中国科技论文,2015,10(12):1364-1368
[33] 鲁杰,石淑兰,刑效攻,杨汝南.NaOH预处理对植物纤维素酶解特性的影响[J].纤维素科学与技术,2004,12(1):3-8
[34] 迟聪聪,柳咪,龚亚辉,李鸿魁,吴养育.碱预处理对玉米秸秆酶解效果的影响[J].陕西科技大学学报,2015,33(5):13-18
[2] 陈婷婷,何剑波,凌宏志.木质纤维素原料氢氧化钠预处理及酶解特性研究[J].酿造,2017,44(1):71-75
[3] 赵晓燕,朱海涛,张桂香,张立金,何磊.超微粉碎预处理对玉米秸秆酶解液中还原糖组分的影响[J].粮油食品科技,2015,23(6):96-98
[4] Jiang S T, Guo N. The steam explosion pretreatment and enzymatic hydrolysis of wheat bran[J].Energy Sources,2016,38(2):295-299
[5] Raveendran S, Mathiyazhakan K, Parameswaran B, Rajeev K S, Ashok P. Physicochemical characterization of alkali pretreated sugarcane tops and optimization of enzymatic saccharification using response surface methodology[J].Renewable Energy,2014,62:362-368
[6] 甄静,李冠杰,李伟,杜志敏,王继雯,尹文兵,陈国参.毛栓孔菌XYG422菌株产漆酶发酵条件优化及对玉米秸秆生物降解的研究[J].菌物学报,2017,36(6):718-729
[7] 张春艳,谭兴和,熊兴耀,苏小军,李清明,王克勤.油菜秸秆60Co-γ辐照降解产物分析[J].湖南农业大学学报(自然科学版),2017,43(1):92-97
[8] 陈静萍,王克勤,彭伟正,熊兴耀. 60Co-γ射线处理稻草秸秆对其纤维质酶解效果的影响[J].激光生物学报,2008,17(1):38-42
[9] 彭姿,谭兴和,熊兴耀,苏小军,张春艳,张涛,王克勤.稀硫酸处理促进辐照芒草木质纤维素酶解糖化条件的优化[J].安徽农业科学,2013,41(31):12455-12457,12461
[10] Siriwattana B. Effect of urea and urea-gamma treatments on cellulose degradation of Thai rice straw and corn stalk[J].Radiation Physics and Chemistry,2002,64(5/6):417-422
[11] 陈亮,武小芬,陈静萍,苏小军,张勇,齐慧,涂晓翼,王克勤.γ射线辐照降解微晶纤维素的研究[J].核农学报,2016,30(9):1731-1737
[12] 彭胜男,陈亮,苏小军,周红丽.氨水协同60Co-γ射线辐照预处理促进芒草酶解糖化的研究[J].中国酿造,2016,35(3):23-26
[13] Wu L, Arakane, M, Ike M, Wada M, Takai T, Gau M, Tokuyasu K. Low temperature alkali pretreatment for improving enzymatic digestibility of sweet sorghum bagasse for ethanol production[J].Bioresourse Technology,2011,102(7):4793-4799
[14] Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D. Determination of structural carbohydrates and lignin in biomass[R]. Golden, USA: National Renewable Energy Laboratory, 2008:6
[15] 陈亮,苏小军,陈静萍,武小芬,熊兴耀,胡秋龙,王克勤.辐照预处理水稻秸秆在高底物浓度下的酶解[J],林产化学与工业,2015,35(2):129-134
[16] 唐洪涛,王锋,李伟明,李安,李彦杰,哈益明.γ射线辐照与NaOH溶液协同预处理对玉米秸秆酶解产糖率及微观结构的影响[J].核农学报,2012,26(3):535-542
[17] 马玉龙,王凤芝,常璇.响应面法优化碱解聚玉米秸秆条件的研究[J].中国粮油学报,2016,31(4):128-132
[18] 武小芬,陈亮,陈静萍,苏小军,齐慧,王克勤,邓明.γ射线辐照降解木聚糖的机理研究[J].核农学报,2017,31(5):889-898
[19] 白献晓,王二柱,朱群,常娟,尹清强,刘超齐,卢富山.碱和蒸汽高压对小麦秸秆中粗纤维降解率的影响[J].河南农业科学,2013,45(11):135-138
[20] Bak J S, Ko J K, Han Y H, Lee B C, Choi Ⅰ G, Kim K H. Improved enzymatic hydrolysis yield of rice straw using electron beam irradiation pretreatment[J].Bioresource Technology,2009,100(3):1285-1290
[21] 沈志强,杨春平,喻国策,王建龙.小麦秸秆的γ辐照与NaOH溶液的协同预处理[J].原子能科学技术,2009,43(4):304-310
[22] 徐红.蒸汽爆破预处理红柳的酶解发酵性能研究[D].南京:南京林业大学,2013:21-22
[23] 马强,顿宝庆,奚亚军,王智,陈朝儒,路明,李桂英.碱处理对甜高粱秸秆渣组成结构及酶解糖化效果的影响[J].作物学报,2014,40(9):1604-1611
[24] 朱圆圆,顾夕梅,朱均均,徐勇,勇强,余世袁.两步碱法预处理对玉米秸秆组分及结构的影响[J].中国科技论文,2015,10(12):1376-1381
[25] Oh S Y, Yoo D Ⅰ, Shin Y, Kim H C, Kim H Y, Chung Y S, Park W H, Youk J H. Crystalline structure analysis of cellulose treated with sodium hydroxide and carbon dioxide by means of X-ray diffraction and FTIR spectroscopy[J]. Carbohydrate Research,2005,340(10):2376-2391
[26] 张荣.基于光谱分析的木材树种识别方法的研究[D]. 南京:南京林业大学,2011:7-8
[27] 范慧青,王喜明,王雅梅.利用傅里叶变换红外光谱法快速测定木材纤维素含量[J].木材加工机械,2014(4):33-37
[28] Peng P, Peng F, Bian J, Xu F, Sun R C, Kennedy J F. Isolation and structural characterization of hemicelluloses from the bamboo species Phyllostachys incarnata Wen[J].Carbohydrate Polymers, 2011,86(2):883-890
[29] 薛晓明,南程慧,吴显坤,陈云霞.三种檀香木的红外光谱比较研究[J].湖北农业科学,2014,53(17):4096-4099
[30] 肖霄,李居南,王志文, 於祥,徐晖.龙竹半纤维素的提取分离及结构表征[J].西北农业学报,2014,23(5):195-201
[31] 鲁南,沈锋,王胜丹,杨桂花,陈嘉川.巨菌草的木素及半纤维素分析[J].中国造纸,2016,35(8):1-6
[32] 王继坤,赵力超,刘欣,曹庸,曹素芳.广林9号桉叶木质素的结构表征[J].中国科技论文,2015,10(12):1364-1368
[33] 鲁杰,石淑兰,刑效攻,杨汝南.NaOH预处理对植物纤维素酶解特性的影响[J].纤维素科学与技术,2004,12(1):3-8
[34] 迟聪聪,柳咪,龚亚辉,李鸿魁,吴养育.碱预处理对玉米秸秆酶解效果的影响[J].陕西科技大学学报,2015,33(5):13-18
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