本草基因组学的学科建设现状与展望
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摘要
本草基因组学是中药学与基因组学的交叉学科,是涵盖药用生物多组学研究和中药与人体相互多组学研究的综合性学科。在药用模式生物、中药合成生物学、中药分子鉴定和药用植物分子育种、药物体内过程组学、中药道地性和药性研究等领域得到了广泛应用,取得了一批标志性成果。随着《中华人民共和国中医药法》《中医药发展战略规划纲要(2016—2030年)》等重要文件的发布,中医药产业进入了全新的、高水平的发展机遇期,复合创新型研究人才的培养是中医药产业发展及医药专业高校教学改革面临的关键问题,该学科的建立对于中医药现代化发展尤为重要,已有多所高等院校开设《本草基因组学》课程,初步形成了特色鲜明的本草基因组学人才培养体系。该文从本草基因组学研究进展、教学开展情况、教材编写背景、主要内容及关键技术、学科特色、学科建设展望方面进行阐述,为本草基因组学的学科建设、人才培养和科学研究提供理论依据和方法学支撑。
Herbgenomics is an interdisciplinary subject between traditional Chinese medicine( TCM)and genomics. It is a comprehensive discipline covering multi-omics research in both medicinal organisms of TCM and the relationship of TCM to human body. It has been widely used in the research fields of medicinal model organisms,synthetic biology of TCM,identification of TCM molecules and breeding of medicinal plant cultivars,pharmacokinetics,and the study on the geoherbalism and medicinal of TCM. With the release of important documents,such as the Law of the People's Republic of China on TCM and the Outline of TCM Development Strategy( 2016—2030),the Chinese medicine industry has entered a new and high-level development opportunity and the herbgenomic research area has got a landmark achievement. The training of well-rounded students and researchers is a key point for the development of TCM industry and the reform of medical colleges and universities. Therefore,the establishment of herbgenomics is particularly important for the modernization of TCM. At present, many colleges and universities have set up the course of Herbgenomics among graduate students and undergraduates,and initially formed a distinctive herbal genomics talent training system. This paper introduces the herbgenomics from the progress of the research,the development of teaching courses,the background of the textbook, the main content and key technologies of the discipline and the prospect of discipline construction, in order to provide theoretical basis and methodological support for the discipline construction,personal training and scientific research of herbgenomics.
Herbgenomics is an interdisciplinary subject between traditional Chinese medicine( TCM)and genomics. It is a comprehensive discipline covering multi-omics research in both medicinal organisms of TCM and the relationship of TCM to human body. It has been widely used in the research fields of medicinal model organisms,synthetic biology of TCM,identification of TCM molecules and breeding of medicinal plant cultivars,pharmacokinetics,and the study on the geoherbalism and medicinal of TCM. With the release of important documents,such as the Law of the People's Republic of China on TCM and the Outline of TCM Development Strategy( 2016—2030),the Chinese medicine industry has entered a new and high-level development opportunity and the herbgenomic research area has got a landmark achievement. The training of well-rounded students and researchers is a key point for the development of TCM industry and the reform of medical colleges and universities. Therefore,the establishment of herbgenomics is particularly important for the modernization of TCM. At present, many colleges and universities have set up the course of Herbgenomics among graduate students and undergraduates,and initially formed a distinctive herbal genomics talent training system. This paper introduces the herbgenomics from the progress of the research,the development of teaching courses,the background of the textbook, the main content and key technologies of the discipline and the prospect of discipline construction, in order to provide theoretical basis and methodological support for the discipline construction,personal training and scientific research of herbgenomics.
引文
[1]CHEN S L,SONG J Y,SUN C,et al. Herbal genomics:examining the biology of traditional medicines[J].Science,2015,347(6219):S27-S29.
[2]陈士林,宋经元.本草基因组学[J].中国中药杂志,2016,41(21):3881-3889.
[3]陈士林.本草基因组学——中药组学的发展与未来[M].北京:科学出版社,2016.
[4]陈士林.本草基因组学[M].北京:科学出版社,2018.
[5]宋经元,徐志超,陈士林.本草基因组学专辑简介[J].中国科学:生命科学,2018,48(4):349-351.
[6]中国药材公司.中国中药资源志要[M].北京:科学出版社,1994.
[7]Sachidanandam R,Weissman D,Schmidt S C,et al. A map of human genome sequence variation containing1. 42 million single nucleotide polymorphisms[J].Nature,2001,409(6822):928-933.
[8]Venter J C,Adams M D,Sutton G G,et al. Shotgun sequencing of the human genome[J]. Science,1998,280(5369):1540-1542.
[9]陈士林,何柳,刘明珠,等.本草基因组方法学研究[J].世界科学技术—中医药现代化,2010,12(3):316-324.
[10]CHEN S L,XU J,LIU C,et al. Genome sequence of the model medicinal mushroom Ganoderma lucidum[J]. Nat Commun,2012,doi:10. 1038/ncomms1923.
[11]XU J,CHU Y,LIAO B,et al. Panax ginseng genome examination for ginsenoside biosynthesis[J].Gigascience,2017,6(11):1-15.
[12]ZHANG D,LI W,XIA E H,et al. The medicinal herb Panax notoginseng genome provides insights into ginsenoside biosynthesis and genome evolution[J]. Mol Plant,2017,10(6):903-907.
[13]XU H,SONG J,LUO H,et al. Analysis of the genome sequence of the medicinal plant Salvia miltiorrhiza[J].Mol Plant,2016,9(6):949-952.
[14]XU Z,Peters R J,Weirather J,et al. Full-length transcriptome sequences and splice variants obtained by a combination of sequencing platforms applied to different root tissues of Salvia miltiorrhiza and tanshinone biosynthesis[J]. Plant J,2015,82(6):951-961.
[15]YAN L,WANG X,LIU H,et al. The genome of Dendrobium officinale illuminates the biology of the important traditional Chinese orchid herb[J]. Mol Plant,2015,8(6):922-934.
[16]SHEN Q,ZHANG L D,TANG K X,et al. The genome of Artemisia annua provides insight into the evolution of asteraceae family and artemisinin biosynthesis[J]. Mol Plant,2018,11(6):776-788.
[17]SONG C,LIU Y F,CHEN S L,et al. The Chrysanthemum nankingense genome provides insights into the evolution and diversification of Chrysanthemum flowers and medicinal traits[J]. Mol Plant,doi:10. 1016/j. molp. 2018. 10. 003.
[18]XU Z,XIN T,Bartels D,et al. Genome analysis of the ancient tracheophyte Selaginella tamariscina reveals evolutionary features relevant to the acquisition of desiccation tolerance[J]. Mol Plant,2018,11(7):983-994.
[19]SUN W,LENG L,YIN Q G,et al. The medicinal plant Andrographis paniculata genome provides insight into biosynthesis of the bioactive diterpenoid neoandrographolide[J]. Plant J,2018,doi:10. 1111/tpj. 14162.
[20]ZHU Y,XU J,SUN C,et al. Chromosome-level genome map provides insights into diverse defense mechanisms in the medicinal fungus Ganoderma sinense[J]. Sci Rep,2015,5:11087.
[21]YUAN Y,JIN X,LIU J,et al. The Gastrodia elata genome provides insights into plant adaptation to heterotrophy[J]. Nat Commun,2018,9(1):1615.
[22]Kellner F,Kim J,Clavijo B J,et al. Genome-guided investigation of plant natural product biosynthesis[J].Plant J,2015,82(4):680-692.
[23]HE L,FU S,XU Z,et al. Hybrid sequencing of fulllength c DNA transcripts of stems and leaves in Dendrobium officinale[J]. Genes(Basel),2017,8(11):E257.
[24]YAO Y F,WANG C S,QIAO J,et al. Metabolic engineering of Escherichia coli for production of salvianic acid A via an artificial biosynthetic pathway[J]. Metab Eng,2013,19:79-87.
[25]浦香东,徐志超,宋经元.基于组学的丹参酮生物合成途径新基因CYP71D375克隆和功能研究[J].中国科学:生命科学,2018,48(4):390-398.
[26]LIU X,CHENG J,ZHANG G,et al. Engineering yeast for the production of breviscapine by genomic analysis and synthetic biology approaches[J]. Nat Commun,2018,9(1):448.
[27]XIANG L,SONG J,XIN T,et al. DNA barcoding the commercial Chinese caterpillar fungus[J]. FEMS Microbiol Lett,2013,347(2):156-162.
[28]ZHAO M,SHI Y,WU L,et al. Rapid authentication of the precious herb saffron by loop-mediated isothermal amplification(LAMP)based on internal transcribed spacer 2(ITS2)sequence[J]. Sci Rep,2016,6:25370.
[29]黄林芳,郑司浩,武拉斌,等.基于化学成分及分子特征中药材肉苁蓉生态型研究[J].中国科学:生命科学,2014,44(3):318-328.
[30]CHEN S L,LUO H,LI Y,et al. 454 EST analysis detects genes putatively involved in ginsenoside biosynthesis in Panax ginseng[J]. Plant Cell Rep,2011,30(9):1593-1601.
[31]王俊,刘霞,张雅琴,等.苍耳子药材及其混伪品ITS2序列鉴定研究[J].世界科学技术—中医药现代化,2014,16(2):329-334.
[32]黎洁文,赵树进.基于条形码ITS2序列的何首乌及其近缘种和混淆品的分子鉴别[J].中国实验方剂学杂志,2015,21(9):80-84.
[33]熊超,李景剑,孙伟,等. HRM结合DNA条形码在苦杏仁对桃仁掺混检测中的应用[J].药学学报,2017,52(4):647-652.
[34]石林春,陈俊,向丽,等.基于ITS2条形码的中药材天南星及其混伪品DNA分子鉴定[J].中国中药杂志,2014,39(12):2176-2179.
[35]张雅琴,宋明,孙伟,等.中药材半夏及其混伪品的DNA条形码鉴定研究[J].世界科学技术—中医药现代化,2014,16(8):1725-1729.
[36]王甜甜.白头翁和翻白草ITS和trn L-F序列分析及鉴别方法的建立[D].长春:吉林大学,2017.
[37]SHI Y H,ZHAO M M,YAO H,et al. Rapidly discriminate commercial medicinal Pulsatilla chinensis(Bge.)Regel from its adulterants using ITS2 barcoding and specific PCR-RFLP assay[J]. Sci Rep,2017,7:40000.
[38]刘霞,林韵涵,陈士林,等.道地药材川麦冬和浙麦冬的生态遗传分化[J].中国实验方剂学杂志,2017,23(17):27-33.
[39]XIANG L,SU Y Y,LI X W,et al. Identification of Fritillariae bulbus from adulterants using ITS2 regions[J]. Plant Gene,2016,7:42-49.
[40]唐科民.昭通天麻DNA条形码研究及药用成分分析[D].昆明:云南中医学院,2013.
[41]王晓明. ITS,rbc L和mat K序列作为中药鸡骨草条形码的研究[D].遵义:遵义医学院,2013.
[42]师玉华,孙伟,方广宏,等.凉茶药材鸡蛋花及其混伪品的DNA条形码鉴定[J].中国中药杂志,2014,39(12):2199-2203.
[43]潘正.基于ITS2条形码和代谢组技术的藏药独一味品质评价研究[D].成都:成都中医药大学,2015.
[44]刘悦,刘川,谭尔,等.基于DNA条形码和1H-NMR代谢组学二维方法的多基原藏药沙棘鉴定[J].中国中药杂志,2016,41(4):578-585.
[45]任瑶瑶,江南屏,刘睿颖,等.藏药臭蒿及其近缘种药材的ITS2 DNA条形码鉴别[J].中国中药杂志,2017,42(7):1395-1400.
[46]刘川,张雨欣,刘悦,等.龙胆科藏药“蒂达”类的DNA条形码鉴定研究[J].中国中药杂志,2016,41(4):567-571.
[47]郭豪杰,丁钰,陈维武,等.藏药材榜嘎及其混伪品的DNA条形码鉴定研究[J].世界中医药,2016,11(5):763-766.
[48]郭力城.壮药金花茶、铁包金的DNA分子鉴定及毛瓣金花茶不同器官表达差异研究[D].武汉:湖北中医药大学,2017.
[49]马双双,王学勇,刘春生,等.基于ITS条形码的维药克孜力乔古鲁克药材的分子鉴定研究[J].环球中医药,2017,10(6):587-591.
[50]何江,陈波,杨伟俊,等.基于ITS2条形码鉴定维药药西瓜及其误用品[J].中国现代中药,2016,18(10):1261-1264.
[51]樊丛照,王果平,李晓瑾,等.维吾尔药材香青兰DNA条形码鉴定方法的研究[J].世界科学技术—中医药现代化,2013,15(3):415-420.
[52]宋明,张雅琴,刘霞,等.基于ITS2序列鉴定苗药金铁锁及其混伪品[J].世界科学技术—中医药现代化,2014,16(8):1730-1734.
[53]聂平,肖炳燚,罗晖明,等.苗药一枝黄花及其混伪品的DNA条形码鉴别[J].食品与药品,2017,19(2):97-101.
[54]魏妮娜.贵州苗药艾纳香DNA条形码的鉴定研究[D].遵义:遵义医学院,2014.
[55]高梓童,王晓玥,刘杨,等.基于ITS2序列的川贝母中成药的鉴定[J].中国科学:生命科学,2018,48(4):482-489.
[56]刘金欣,魏妙洁,谢丽芳,等.基于DNA metabarcoding技术的如意金黄散处方成分鉴定研究[J].中国科学:生命科学,2018,48(4):490-497.
[57]陈士林.中国药典中药材DNA条形码标准序列[M].北京:科学出版社,2015.
[58]陈士林.中药DNA条形码分子鉴定[M].北京:人民卫生出版社,2012.
[59]国家药典委员会.中华人民共和国药典. 2010年版第三增补本[M].北京:中国医药科技出版社,2014.
[60]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015.
[61]British Pharmacopoeia Commission. British Pharmacopoeia[M]. 2017 edition. London:TSO,2016.
[62]王丽芝,王海英,浦香东,等.基于紫芝基因组的倍半萜合酶基因的发掘与功能鉴定[J].中国科学:生命科学,2018,48(4):447-454.
[63]陈军峰,李卿,王芸,等.高通量测序解析大青叶有效成分的生物合成途径[J].中国科学:生命科学,2018,48(4):412-422.
[64]Graham I A,Besser K,Blumer S,et al. The genetic map of Artemisia annua L. identifies loci affecting yield of the antimalarial drug artemisinin[J]. Science,2010,327(5963):328-331.
[65]董林林,陈中坚,王勇,等.药用植物DNA标记辅助育种(一):三七抗病品种选育研究[J].中国中药杂志,2017,42(1):56-62.
[66]陈中坚,马小涵,董林林,等.药用植物DNA标记辅助育种(三):三七新品种——“苗乡抗七1号”的抗病性评价[J].中国中药杂志,2017,42(11):2046-2051.
[67]尉广飞,董林林,陈士林,等.本草基因组学在中药材新品种选育中的应用[J].中国实验方剂学杂志,2018,24(23):18-28.
[68]陈尘,曹晓燕,代文平,等.基于丹参基因组的COⅠ1基因抗虫与次生代谢调控功能研究[J].中国科学:生命科学,2018,48(4):399-411.
[69]初旸,徐然,苏贺,等.人参NBS-LRR抗病基因家族全基因组分析[J].中国科学:生命科学,2018,48(4):423-435.
[70]陈士林,朱孝轩,陈晓辰,等.现代生物技术在人参属药用植物研究中的应用[J].中国中药杂志,2013,38(5):633-639.
[71]赓迪,方明月,李德利,等.中药与肠道微环境相互作用研究进展[J].中国科学:生命科学,2018,48(4):379-389.
[72]刘婉菁,张建红,罗红梅.中草药表观基因组学研究现状与展望[J].中国科学:生命科学,2018,48(4):365-378.
[73]杜涧超,梁竹,许剑涛,等.植物来源的磷脂酰胆碱可介导抗肺纤维化小RNA(HJT-sRNA-m7)进入哺乳动物细胞[J].中国科学:生命科学,2018,48(4):469-481.
[74]杨洁.大株红景天注射液治疗特发性肺纤维化的临床研究[J].中医临床研究,2014,6(17):8-9.
[75]郭慧,崔扬,王秋红,等.基于代谢组学技术的中药复方研究近况[J].中国实验方剂学杂志,2017,23(1):213-219.
[76]尹晓月,王维东,钱朝菊,等.基于UPLC-MS的沙米代谢组学分析[J].中国实验方剂学杂志,2018,24(15):51-56.
[77]赵洪伟,刘树民,张宁,等.玄参对脾虚水湿不化大鼠模型的影响及其肝脏代谢组学分析[J].中国实验方剂学杂志,2017,23(20):82-89.
[78]张剑霜,喻浩,钟欣,等.基于GC-MS代谢组学技术比较冬虫夏草与蝉花的质量[J].中国实验方剂学杂志,2018,24(18):23-29.
[79]李光,李宜航,吕亚娜,等.基于代谢组学技术探讨特色傣药肾茶的“雅解”作用机制[J].中国科学:生命科学,2018,48(4):455-468.
[80]何晓瑾,金实,周学平,等.创新人才培养视阈下的大学教学模式变革-基于PBL模式的教学实践研究[J].江苏高教,2016(6):99-101.
[81]秦宣云,李军英,郑建梅,等.融入启发式、探究式、讨论式、参与式教学方法的高等数学课程课堂教学改革的研究与实践[J].大学教育,2015(5):95-96.
[82]安永磊,高淑贞,刘南,等.开放性创新实验教学模式优化与实践[J].实验技术与管理,2016,33(8):21-23.
[83]吴晓毅,刘长利,高伟,等.《药用植物学》慕课的学习实践与教学启示[J].中国实验方剂学杂志,2017,23(16):16-19.
[84]刘春生.论《药用植物学》教学改革[J].中国实验方剂学杂志,2017,23(16):1-3.
[85]Sanger F,Air G M,Barrell B G,et al. Nucleotide sequence of bacteriophageφX174 DNA[J]. Nature,1977,265(5596):687-695.
[86]Initiative A G. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana[J]. Nature,2000,408(6814):796-815.
[87]杨俏俏,黄林芳.基于文献计量学的药用植物基因组学国际研究态势分析[J].中国科学:生命科学,2018,48(4):498-508.
[2]陈士林,宋经元.本草基因组学[J].中国中药杂志,2016,41(21):3881-3889.
[3]陈士林.本草基因组学——中药组学的发展与未来[M].北京:科学出版社,2016.
[4]陈士林.本草基因组学[M].北京:科学出版社,2018.
[5]宋经元,徐志超,陈士林.本草基因组学专辑简介[J].中国科学:生命科学,2018,48(4):349-351.
[6]中国药材公司.中国中药资源志要[M].北京:科学出版社,1994.
[7]Sachidanandam R,Weissman D,Schmidt S C,et al. A map of human genome sequence variation containing1. 42 million single nucleotide polymorphisms[J].Nature,2001,409(6822):928-933.
[8]Venter J C,Adams M D,Sutton G G,et al. Shotgun sequencing of the human genome[J]. Science,1998,280(5369):1540-1542.
[9]陈士林,何柳,刘明珠,等.本草基因组方法学研究[J].世界科学技术—中医药现代化,2010,12(3):316-324.
[10]CHEN S L,XU J,LIU C,et al. Genome sequence of the model medicinal mushroom Ganoderma lucidum[J]. Nat Commun,2012,doi:10. 1038/ncomms1923.
[11]XU J,CHU Y,LIAO B,et al. Panax ginseng genome examination for ginsenoside biosynthesis[J].Gigascience,2017,6(11):1-15.
[12]ZHANG D,LI W,XIA E H,et al. The medicinal herb Panax notoginseng genome provides insights into ginsenoside biosynthesis and genome evolution[J]. Mol Plant,2017,10(6):903-907.
[13]XU H,SONG J,LUO H,et al. Analysis of the genome sequence of the medicinal plant Salvia miltiorrhiza[J].Mol Plant,2016,9(6):949-952.
[14]XU Z,Peters R J,Weirather J,et al. Full-length transcriptome sequences and splice variants obtained by a combination of sequencing platforms applied to different root tissues of Salvia miltiorrhiza and tanshinone biosynthesis[J]. Plant J,2015,82(6):951-961.
[15]YAN L,WANG X,LIU H,et al. The genome of Dendrobium officinale illuminates the biology of the important traditional Chinese orchid herb[J]. Mol Plant,2015,8(6):922-934.
[16]SHEN Q,ZHANG L D,TANG K X,et al. The genome of Artemisia annua provides insight into the evolution of asteraceae family and artemisinin biosynthesis[J]. Mol Plant,2018,11(6):776-788.
[17]SONG C,LIU Y F,CHEN S L,et al. The Chrysanthemum nankingense genome provides insights into the evolution and diversification of Chrysanthemum flowers and medicinal traits[J]. Mol Plant,doi:10. 1016/j. molp. 2018. 10. 003.
[18]XU Z,XIN T,Bartels D,et al. Genome analysis of the ancient tracheophyte Selaginella tamariscina reveals evolutionary features relevant to the acquisition of desiccation tolerance[J]. Mol Plant,2018,11(7):983-994.
[19]SUN W,LENG L,YIN Q G,et al. The medicinal plant Andrographis paniculata genome provides insight into biosynthesis of the bioactive diterpenoid neoandrographolide[J]. Plant J,2018,doi:10. 1111/tpj. 14162.
[20]ZHU Y,XU J,SUN C,et al. Chromosome-level genome map provides insights into diverse defense mechanisms in the medicinal fungus Ganoderma sinense[J]. Sci Rep,2015,5:11087.
[21]YUAN Y,JIN X,LIU J,et al. The Gastrodia elata genome provides insights into plant adaptation to heterotrophy[J]. Nat Commun,2018,9(1):1615.
[22]Kellner F,Kim J,Clavijo B J,et al. Genome-guided investigation of plant natural product biosynthesis[J].Plant J,2015,82(4):680-692.
[23]HE L,FU S,XU Z,et al. Hybrid sequencing of fulllength c DNA transcripts of stems and leaves in Dendrobium officinale[J]. Genes(Basel),2017,8(11):E257.
[24]YAO Y F,WANG C S,QIAO J,et al. Metabolic engineering of Escherichia coli for production of salvianic acid A via an artificial biosynthetic pathway[J]. Metab Eng,2013,19:79-87.
[25]浦香东,徐志超,宋经元.基于组学的丹参酮生物合成途径新基因CYP71D375克隆和功能研究[J].中国科学:生命科学,2018,48(4):390-398.
[26]LIU X,CHENG J,ZHANG G,et al. Engineering yeast for the production of breviscapine by genomic analysis and synthetic biology approaches[J]. Nat Commun,2018,9(1):448.
[27]XIANG L,SONG J,XIN T,et al. DNA barcoding the commercial Chinese caterpillar fungus[J]. FEMS Microbiol Lett,2013,347(2):156-162.
[28]ZHAO M,SHI Y,WU L,et al. Rapid authentication of the precious herb saffron by loop-mediated isothermal amplification(LAMP)based on internal transcribed spacer 2(ITS2)sequence[J]. Sci Rep,2016,6:25370.
[29]黄林芳,郑司浩,武拉斌,等.基于化学成分及分子特征中药材肉苁蓉生态型研究[J].中国科学:生命科学,2014,44(3):318-328.
[30]CHEN S L,LUO H,LI Y,et al. 454 EST analysis detects genes putatively involved in ginsenoside biosynthesis in Panax ginseng[J]. Plant Cell Rep,2011,30(9):1593-1601.
[31]王俊,刘霞,张雅琴,等.苍耳子药材及其混伪品ITS2序列鉴定研究[J].世界科学技术—中医药现代化,2014,16(2):329-334.
[32]黎洁文,赵树进.基于条形码ITS2序列的何首乌及其近缘种和混淆品的分子鉴别[J].中国实验方剂学杂志,2015,21(9):80-84.
[33]熊超,李景剑,孙伟,等. HRM结合DNA条形码在苦杏仁对桃仁掺混检测中的应用[J].药学学报,2017,52(4):647-652.
[34]石林春,陈俊,向丽,等.基于ITS2条形码的中药材天南星及其混伪品DNA分子鉴定[J].中国中药杂志,2014,39(12):2176-2179.
[35]张雅琴,宋明,孙伟,等.中药材半夏及其混伪品的DNA条形码鉴定研究[J].世界科学技术—中医药现代化,2014,16(8):1725-1729.
[36]王甜甜.白头翁和翻白草ITS和trn L-F序列分析及鉴别方法的建立[D].长春:吉林大学,2017.
[37]SHI Y H,ZHAO M M,YAO H,et al. Rapidly discriminate commercial medicinal Pulsatilla chinensis(Bge.)Regel from its adulterants using ITS2 barcoding and specific PCR-RFLP assay[J]. Sci Rep,2017,7:40000.
[38]刘霞,林韵涵,陈士林,等.道地药材川麦冬和浙麦冬的生态遗传分化[J].中国实验方剂学杂志,2017,23(17):27-33.
[39]XIANG L,SU Y Y,LI X W,et al. Identification of Fritillariae bulbus from adulterants using ITS2 regions[J]. Plant Gene,2016,7:42-49.
[40]唐科民.昭通天麻DNA条形码研究及药用成分分析[D].昆明:云南中医学院,2013.
[41]王晓明. ITS,rbc L和mat K序列作为中药鸡骨草条形码的研究[D].遵义:遵义医学院,2013.
[42]师玉华,孙伟,方广宏,等.凉茶药材鸡蛋花及其混伪品的DNA条形码鉴定[J].中国中药杂志,2014,39(12):2199-2203.
[43]潘正.基于ITS2条形码和代谢组技术的藏药独一味品质评价研究[D].成都:成都中医药大学,2015.
[44]刘悦,刘川,谭尔,等.基于DNA条形码和1H-NMR代谢组学二维方法的多基原藏药沙棘鉴定[J].中国中药杂志,2016,41(4):578-585.
[45]任瑶瑶,江南屏,刘睿颖,等.藏药臭蒿及其近缘种药材的ITS2 DNA条形码鉴别[J].中国中药杂志,2017,42(7):1395-1400.
[46]刘川,张雨欣,刘悦,等.龙胆科藏药“蒂达”类的DNA条形码鉴定研究[J].中国中药杂志,2016,41(4):567-571.
[47]郭豪杰,丁钰,陈维武,等.藏药材榜嘎及其混伪品的DNA条形码鉴定研究[J].世界中医药,2016,11(5):763-766.
[48]郭力城.壮药金花茶、铁包金的DNA分子鉴定及毛瓣金花茶不同器官表达差异研究[D].武汉:湖北中医药大学,2017.
[49]马双双,王学勇,刘春生,等.基于ITS条形码的维药克孜力乔古鲁克药材的分子鉴定研究[J].环球中医药,2017,10(6):587-591.
[50]何江,陈波,杨伟俊,等.基于ITS2条形码鉴定维药药西瓜及其误用品[J].中国现代中药,2016,18(10):1261-1264.
[51]樊丛照,王果平,李晓瑾,等.维吾尔药材香青兰DNA条形码鉴定方法的研究[J].世界科学技术—中医药现代化,2013,15(3):415-420.
[52]宋明,张雅琴,刘霞,等.基于ITS2序列鉴定苗药金铁锁及其混伪品[J].世界科学技术—中医药现代化,2014,16(8):1730-1734.
[53]聂平,肖炳燚,罗晖明,等.苗药一枝黄花及其混伪品的DNA条形码鉴别[J].食品与药品,2017,19(2):97-101.
[54]魏妮娜.贵州苗药艾纳香DNA条形码的鉴定研究[D].遵义:遵义医学院,2014.
[55]高梓童,王晓玥,刘杨,等.基于ITS2序列的川贝母中成药的鉴定[J].中国科学:生命科学,2018,48(4):482-489.
[56]刘金欣,魏妙洁,谢丽芳,等.基于DNA metabarcoding技术的如意金黄散处方成分鉴定研究[J].中国科学:生命科学,2018,48(4):490-497.
[57]陈士林.中国药典中药材DNA条形码标准序列[M].北京:科学出版社,2015.
[58]陈士林.中药DNA条形码分子鉴定[M].北京:人民卫生出版社,2012.
[59]国家药典委员会.中华人民共和国药典. 2010年版第三增补本[M].北京:中国医药科技出版社,2014.
[60]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015.
[61]British Pharmacopoeia Commission. British Pharmacopoeia[M]. 2017 edition. London:TSO,2016.
[62]王丽芝,王海英,浦香东,等.基于紫芝基因组的倍半萜合酶基因的发掘与功能鉴定[J].中国科学:生命科学,2018,48(4):447-454.
[63]陈军峰,李卿,王芸,等.高通量测序解析大青叶有效成分的生物合成途径[J].中国科学:生命科学,2018,48(4):412-422.
[64]Graham I A,Besser K,Blumer S,et al. The genetic map of Artemisia annua L. identifies loci affecting yield of the antimalarial drug artemisinin[J]. Science,2010,327(5963):328-331.
[65]董林林,陈中坚,王勇,等.药用植物DNA标记辅助育种(一):三七抗病品种选育研究[J].中国中药杂志,2017,42(1):56-62.
[66]陈中坚,马小涵,董林林,等.药用植物DNA标记辅助育种(三):三七新品种——“苗乡抗七1号”的抗病性评价[J].中国中药杂志,2017,42(11):2046-2051.
[67]尉广飞,董林林,陈士林,等.本草基因组学在中药材新品种选育中的应用[J].中国实验方剂学杂志,2018,24(23):18-28.
[68]陈尘,曹晓燕,代文平,等.基于丹参基因组的COⅠ1基因抗虫与次生代谢调控功能研究[J].中国科学:生命科学,2018,48(4):399-411.
[69]初旸,徐然,苏贺,等.人参NBS-LRR抗病基因家族全基因组分析[J].中国科学:生命科学,2018,48(4):423-435.
[70]陈士林,朱孝轩,陈晓辰,等.现代生物技术在人参属药用植物研究中的应用[J].中国中药杂志,2013,38(5):633-639.
[71]赓迪,方明月,李德利,等.中药与肠道微环境相互作用研究进展[J].中国科学:生命科学,2018,48(4):379-389.
[72]刘婉菁,张建红,罗红梅.中草药表观基因组学研究现状与展望[J].中国科学:生命科学,2018,48(4):365-378.
[73]杜涧超,梁竹,许剑涛,等.植物来源的磷脂酰胆碱可介导抗肺纤维化小RNA(HJT-sRNA-m7)进入哺乳动物细胞[J].中国科学:生命科学,2018,48(4):469-481.
[74]杨洁.大株红景天注射液治疗特发性肺纤维化的临床研究[J].中医临床研究,2014,6(17):8-9.
[75]郭慧,崔扬,王秋红,等.基于代谢组学技术的中药复方研究近况[J].中国实验方剂学杂志,2017,23(1):213-219.
[76]尹晓月,王维东,钱朝菊,等.基于UPLC-MS的沙米代谢组学分析[J].中国实验方剂学杂志,2018,24(15):51-56.
[77]赵洪伟,刘树民,张宁,等.玄参对脾虚水湿不化大鼠模型的影响及其肝脏代谢组学分析[J].中国实验方剂学杂志,2017,23(20):82-89.
[78]张剑霜,喻浩,钟欣,等.基于GC-MS代谢组学技术比较冬虫夏草与蝉花的质量[J].中国实验方剂学杂志,2018,24(18):23-29.
[79]李光,李宜航,吕亚娜,等.基于代谢组学技术探讨特色傣药肾茶的“雅解”作用机制[J].中国科学:生命科学,2018,48(4):455-468.
[80]何晓瑾,金实,周学平,等.创新人才培养视阈下的大学教学模式变革-基于PBL模式的教学实践研究[J].江苏高教,2016(6):99-101.
[81]秦宣云,李军英,郑建梅,等.融入启发式、探究式、讨论式、参与式教学方法的高等数学课程课堂教学改革的研究与实践[J].大学教育,2015(5):95-96.
[82]安永磊,高淑贞,刘南,等.开放性创新实验教学模式优化与实践[J].实验技术与管理,2016,33(8):21-23.
[83]吴晓毅,刘长利,高伟,等.《药用植物学》慕课的学习实践与教学启示[J].中国实验方剂学杂志,2017,23(16):16-19.
[84]刘春生.论《药用植物学》教学改革[J].中国实验方剂学杂志,2017,23(16):1-3.
[85]Sanger F,Air G M,Barrell B G,et al. Nucleotide sequence of bacteriophageφX174 DNA[J]. Nature,1977,265(5596):687-695.
[86]Initiative A G. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana[J]. Nature,2000,408(6814):796-815.
[87]杨俏俏,黄林芳.基于文献计量学的药用植物基因组学国际研究态势分析[J].中国科学:生命科学,2018,48(4):498-508.
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