结直肠癌组织中SCD-1的表达及临床意义
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摘要
目的探讨结直肠癌组织中酰基辅酶A去饱和酶1(SCD-1)的表达及其临床意义。方法收集2014年12月至2017年12月手术切除的77对结直肠癌组织和癌旁组织,采用免疫组化SP法检测上述组织中SCD-1的表达情况,分析其表达差异及与临床病理特征的关系。结果 SCD-1主要表达于细胞浆和胞膜。在结直肠癌组织中SCD-1的阳性表达率为66.2%(51/77),高于癌旁正常组织的13.0%(10/77),差异有统计学意义(P<0.05)。SCD-1表达与性别、年龄、分化程度和部位均无关(P>0.05),而与肿瘤大小、浸润深度、淋巴结转移和TNM分期有关(P<0.05)。其中肿瘤较大(≥5 cm)、浸润较深(T_3+T_4)、淋巴结转移和分期较晚(Ⅲ+Ⅳ)的组织中SCD-1阳性表达率分别为80.6%(29/36)、72.9%(43/59)、78.6%(33/42)和78.0%(32/41),均高于肿瘤较小(<5 cm)、浸润较浅(T_1+T_2)、无淋巴结转移和分期较早(Ⅰ+Ⅱ)组织的53.7%(22/41)、44.4%(8/18)、51.4%(18/35)和52.8%(19/36),差异均有统计学意义(P<0.05)。结论 SCD-1蛋白高表达可能参与结直肠癌的发生、发展,SCD-1表达可能是肿瘤进展的潜在标志物。
Objective To investigate the expression and clinical significance of stearoy1-COA desaturase-1(SCD-1) in colorectal cancer tissues. Methods Seventy-seven pairs of colorectal cancer tissues and adjacent tissues were collected from December 2014 to December 2017. The expression of SCD-1 in the above tissues was detected by immunohistochemical SP method. The difference of SCD-1 expression and its relationship with clinicopathological features were analyzed. Results SCD-1 was mainly expressed in cytoplasm and cell membrane by immunohistochemistry. The positive rate of SCD-1 expression was 66.2%(51/77) in colorectal cancer tissues, higher than 13.0%(10/77) of adjacent tissues with significant difference(P<0.05). The expression of SCD-1 was not related to gender, age, differentiation and tumor location(P>0.05), but associated with tumor size, depth of invasion, lymphnode metastasis and TNM staging(P<0.05). The positive expression rates of SCD-1 in tumors of ≥5 cm in size, deep infiltration(T_3+T_4), lymph node metastasis and late staging(Ⅲ+Ⅳ) were 80.6%(29/36), 72.9%(43/59), 78.6%(33/42) and 78.0%(32/41), higher than 53.7%(22/41), 44.4%(8/18), 51.4%(18/35) and 52.8%(19/36) in corresponding tumors of small tumors(<5 cm), superficial infiltration(T_1+T_2), non-lymph node metastasis and early staging(Ⅰ+Ⅱ) with statistical significance(P<0.05). Conclusion The elevated expression of SCD-1 may be involved in the development of colorectal cancer, and SCD-1 may be a potential marker for tumor progression.
Objective To investigate the expression and clinical significance of stearoy1-COA desaturase-1(SCD-1) in colorectal cancer tissues. Methods Seventy-seven pairs of colorectal cancer tissues and adjacent tissues were collected from December 2014 to December 2017. The expression of SCD-1 in the above tissues was detected by immunohistochemical SP method. The difference of SCD-1 expression and its relationship with clinicopathological features were analyzed. Results SCD-1 was mainly expressed in cytoplasm and cell membrane by immunohistochemistry. The positive rate of SCD-1 expression was 66.2%(51/77) in colorectal cancer tissues, higher than 13.0%(10/77) of adjacent tissues with significant difference(P<0.05). The expression of SCD-1 was not related to gender, age, differentiation and tumor location(P>0.05), but associated with tumor size, depth of invasion, lymphnode metastasis and TNM staging(P<0.05). The positive expression rates of SCD-1 in tumors of ≥5 cm in size, deep infiltration(T_3+T_4), lymph node metastasis and late staging(Ⅲ+Ⅳ) were 80.6%(29/36), 72.9%(43/59), 78.6%(33/42) and 78.0%(32/41), higher than 53.7%(22/41), 44.4%(8/18), 51.4%(18/35) and 52.8%(19/36) in corresponding tumors of small tumors(<5 cm), superficial infiltration(T_1+T_2), non-lymph node metastasis and early staging(Ⅰ+Ⅱ) with statistical significance(P<0.05). Conclusion The elevated expression of SCD-1 may be involved in the development of colorectal cancer, and SCD-1 may be a potential marker for tumor progression.
引文
[1] Siegel RL, Miller KD, Jemal A. Cancer statistics, 2017 [J]. CA Cancer J Clin, 2015, 60(5):277-300.
[2] Siegel R, Desantis C, Jemal A. Colorectal cancer statistics, 2014 [J]. CA Cancer J Clin, 2014, 64(2):104-117.
[3] Presler M, Wojtczyk-Miaskowska A, Schlichtholz B, et al. Increased expression of the gene encoding stearoyl-CoA desaturase 1 in human bladder cancer [J]. Mol Cell Biochem, 2018, 447(1-2): 217-224.
[4] Angelucci C, D'Alessio A, Iacopino F, et al. Pivotal role of human stearoyl-CoA desaturases (SCD1 and 5) in breast cancer progression: oleic acid-based effect of SCD1 on cell migration and a novel pro-cell survival role for SCD5 [J]. Oncotarget, 2018, 9(36): 24364-24380.
[5] Colacino JA, Mcdermott SP, Sartor MA, et al. Transcriptomic profiling of curcumin-treated human breast stem cells identifies a role for stearoyl-coa desaturase in breast cancer prevention [J]. Breast Cancer Res Treat, 2016, 158(1): 29-41.
[6] Noto A, De VC, Pisanu ME, et al. Stearoyl-CoA-desaturase 1 regulates lung cancer stemness via stabilization and nuclear localization of YAP/TAZ [J]. Oncogene, 2017, 36(32): 4671-4672.
[7] Qu Q, Zeng F, Liu X, et al. Fatty acid oxidation and carnitine palmitoyltransferase I: emerging therapeutic targets in cancer [J/OL]. Cell Death Dis, 2016[2018-05-12]. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/27195673.
[8] Barrera G, Gentile F, Pizzimenti S, et al. Mitochondrial dysfunction in cancer and neurodegenerative diseases: spotlight on fatty acid oxidation and lipoperoxidation products [J/OL]. Antioxidants, 2016[2018-03-22]. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4808756.
[9] 杨君, 张彩凤, 秦瑞英, 等. SCD-1在宫颈癌中的表达及其表达下调对宫颈癌细胞增殖和凋亡的影响[J].中国病理生理杂志, 2013, 29(11): 1972-1977.
[10] 陈文彬, 吴德谋, 邹永平. 胃癌组织中SCD1的表达及其对胃癌细胞增殖侵袭能力的影响[J]. 广西医科大学学报, 2017, 34(9): 1285-1288.
[11] 黄光明, 沈薇. SCD1对肝癌SMMC-7721细胞脂质代谢的影响及可能机制[J]. 肿瘤学杂志, 2017, 23(8): 653-657.
[12] Angelucci C, D'Alessio A, Iacopino F, et al. Pivotal role of human stearoyl-CoA desaturases (SCD1 and 5) in breast cancer progression: oleic acid-based effect of SCD1 on cell migration and a novel pro-cell survival role for SCD5[J]. Oncotarget, 2018, 9(36): 24364-24380.
[13] Southam AD, Khanim FL, Hayden RE, et al. Drug redeployment to kill leukaemia and lymphoma cells by disrupting SCD1-mediated synthesis of monounsaturated fatty acids [J]. Cancer Res, 2015, 75(12): 2530-2540.
[14] Dai S, Yan Y, Xu Z, et al. SCD1 confers temozolomide resistance to human glioma cells via the Akt/GSK3β/β-Catenin signaling axis [J/OL]. Front Pharmacol,2017[2018-06-30].http://www.ncbi.nlm.nih.gov/pubmed/29354058.
[15] Ralston JC, Mutch DSM. SCD1 inhibition during 3T3-L1 adipocyte differentiation remodels triacylglycerol, diacylglycerol and phospholipid fatty acid composition [J]. Prostaglandins Leukot Essent Fatty Acids, 2015, 98: 29-37.
[16] 周晓黎, 舒磊, 廖艳, 等. PI3K/AKT通路在低氧环境下对结肠癌细胞HIF-1α及糖酵解的作用[J]. 华中科技大学学报(医学版), 2018, 47(2): 203-206.
[17] Holder AM, Gonzalez-Angulo AM, Chen H, et al. High stearoyl-CoA desaturase 1 expression is associated with shorter survival in breast cancer patients [J]. Breast Cancer Res Treat, 2013,137(1): 319-327.
[18] 郜辉, 冯应勤, 朱金峰, 等. 抑制酰基-辅酶A去饱和酶1表达对食管鳞癌细胞增殖和凋亡的影响及其分子机制探讨[J]. 肿瘤, 2012, 32(9): 681-688.
[2] Siegel R, Desantis C, Jemal A. Colorectal cancer statistics, 2014 [J]. CA Cancer J Clin, 2014, 64(2):104-117.
[3] Presler M, Wojtczyk-Miaskowska A, Schlichtholz B, et al. Increased expression of the gene encoding stearoyl-CoA desaturase 1 in human bladder cancer [J]. Mol Cell Biochem, 2018, 447(1-2): 217-224.
[4] Angelucci C, D'Alessio A, Iacopino F, et al. Pivotal role of human stearoyl-CoA desaturases (SCD1 and 5) in breast cancer progression: oleic acid-based effect of SCD1 on cell migration and a novel pro-cell survival role for SCD5 [J]. Oncotarget, 2018, 9(36): 24364-24380.
[5] Colacino JA, Mcdermott SP, Sartor MA, et al. Transcriptomic profiling of curcumin-treated human breast stem cells identifies a role for stearoyl-coa desaturase in breast cancer prevention [J]. Breast Cancer Res Treat, 2016, 158(1): 29-41.
[6] Noto A, De VC, Pisanu ME, et al. Stearoyl-CoA-desaturase 1 regulates lung cancer stemness via stabilization and nuclear localization of YAP/TAZ [J]. Oncogene, 2017, 36(32): 4671-4672.
[7] Qu Q, Zeng F, Liu X, et al. Fatty acid oxidation and carnitine palmitoyltransferase I: emerging therapeutic targets in cancer [J/OL]. Cell Death Dis, 2016[2018-05-12]. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/27195673.
[8] Barrera G, Gentile F, Pizzimenti S, et al. Mitochondrial dysfunction in cancer and neurodegenerative diseases: spotlight on fatty acid oxidation and lipoperoxidation products [J/OL]. Antioxidants, 2016[2018-03-22]. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4808756.
[9] 杨君, 张彩凤, 秦瑞英, 等. SCD-1在宫颈癌中的表达及其表达下调对宫颈癌细胞增殖和凋亡的影响[J].中国病理生理杂志, 2013, 29(11): 1972-1977.
[10] 陈文彬, 吴德谋, 邹永平. 胃癌组织中SCD1的表达及其对胃癌细胞增殖侵袭能力的影响[J]. 广西医科大学学报, 2017, 34(9): 1285-1288.
[11] 黄光明, 沈薇. SCD1对肝癌SMMC-7721细胞脂质代谢的影响及可能机制[J]. 肿瘤学杂志, 2017, 23(8): 653-657.
[12] Angelucci C, D'Alessio A, Iacopino F, et al. Pivotal role of human stearoyl-CoA desaturases (SCD1 and 5) in breast cancer progression: oleic acid-based effect of SCD1 on cell migration and a novel pro-cell survival role for SCD5[J]. Oncotarget, 2018, 9(36): 24364-24380.
[13] Southam AD, Khanim FL, Hayden RE, et al. Drug redeployment to kill leukaemia and lymphoma cells by disrupting SCD1-mediated synthesis of monounsaturated fatty acids [J]. Cancer Res, 2015, 75(12): 2530-2540.
[14] Dai S, Yan Y, Xu Z, et al. SCD1 confers temozolomide resistance to human glioma cells via the Akt/GSK3β/β-Catenin signaling axis [J/OL]. Front Pharmacol,2017[2018-06-30].http://www.ncbi.nlm.nih.gov/pubmed/29354058.
[15] Ralston JC, Mutch DSM. SCD1 inhibition during 3T3-L1 adipocyte differentiation remodels triacylglycerol, diacylglycerol and phospholipid fatty acid composition [J]. Prostaglandins Leukot Essent Fatty Acids, 2015, 98: 29-37.
[16] 周晓黎, 舒磊, 廖艳, 等. PI3K/AKT通路在低氧环境下对结肠癌细胞HIF-1α及糖酵解的作用[J]. 华中科技大学学报(医学版), 2018, 47(2): 203-206.
[17] Holder AM, Gonzalez-Angulo AM, Chen H, et al. High stearoyl-CoA desaturase 1 expression is associated with shorter survival in breast cancer patients [J]. Breast Cancer Res Treat, 2013,137(1): 319-327.
[18] 郜辉, 冯应勤, 朱金峰, 等. 抑制酰基-辅酶A去饱和酶1表达对食管鳞癌细胞增殖和凋亡的影响及其分子机制探讨[J]. 肿瘤, 2012, 32(9): 681-688.
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