间苯三酚联合运动对原发性痛经大鼠血清学指标与子宫组织MMP-3与MMP-9表达的影响
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摘要
目的:探讨间苯三酚联合运动对原发性痛经大鼠血清学指标与子宫组织MMP-3与MMP-9表达的影响。方法:4月龄未孕动情间期SD雌性大鼠24只,随即分为对照组、模型组和治疗组(注射间苯三酚+运动干预),利用缩宫素建立原发性痛经模型。比较各组大鼠扭体情况,测定各组大鼠血清血清雌二醇(E2)、前列腺素E2(PGE2)、前列腺素F2α(PGF2α)、雌激素(P)的含量,Real-time PCR和Western blot方法检测分别检测子宫组织MMP-3、MMP-9基因及蛋白表达。结果:间苯三酚联合运动干预,能够显著上调原发性痛经大鼠血清PGE2水平,降低血清E2、PGF2α水平比值,降低扭体反应次数,P <0. 05。与对照组相比,原发性痛经大鼠子宫组织MMP-3、MMP-9基因及蛋白表达水平显著升高,P <0. 05;但是给予间苯三酚联合运动干预后,原发性痛经大鼠子宫组织MMP-3、MMP-9基因及蛋白表达水平显著降低,P <0. 05。结论:间苯三酚联合运动可拮抗缩宫素致大鼠实验性痛经。
Objective: To investigate the effects of phloroglucin combined with training on serological indicators and expression of MMP-3 and MMP-9 in uterus of primary dysmenorrhea rats. Methods: Twenty-four female SD rats were randomly divided into three groups: control group,model group and treatment group. Oxytocin was used to establish primary dysmenorrhea model. The levels of estradiol( E 2),prostaglandin E 2( PGE2),prostaglandin F 2 α( PGF2 α) and estrogen( P) were measured. The expression of MMP-3,MMP-9 gene and protein were detected by Real-time PCR and Western blot. Results: In combination with exercise intervention,the level of serum PGE2 in primary dysmenorrhea rats was upregulated,the ratio of serum E2 PGF2 α was decreased,and the times of writhing reaction was decreased( P < 0. 05). Compared with the control group,the expression of MMP-3,MMP-9 gene and protein in uterus of primary dysmenorrhea rats was significantly increased( P < 0. 05). However,the expression of MMP-3,MMP-9 gene and protein in uterine tissues of primary dysmenorrhea rats decreased significantly( P < 0. 05) after treatment with phloroglucinol combined with exercise. Conclusion: Phloroglucinol combined with exercise can antagonize experimental dysmenorrhea induced by oxytocin in rats.
Objective: To investigate the effects of phloroglucin combined with training on serological indicators and expression of MMP-3 and MMP-9 in uterus of primary dysmenorrhea rats. Methods: Twenty-four female SD rats were randomly divided into three groups: control group,model group and treatment group. Oxytocin was used to establish primary dysmenorrhea model. The levels of estradiol( E 2),prostaglandin E 2( PGE2),prostaglandin F 2 α( PGF2 α) and estrogen( P) were measured. The expression of MMP-3,MMP-9 gene and protein were detected by Real-time PCR and Western blot. Results: In combination with exercise intervention,the level of serum PGE2 in primary dysmenorrhea rats was upregulated,the ratio of serum E2 PGF2 α was decreased,and the times of writhing reaction was decreased( P < 0. 05). Compared with the control group,the expression of MMP-3,MMP-9 gene and protein in uterus of primary dysmenorrhea rats was significantly increased( P < 0. 05). However,the expression of MMP-3,MMP-9 gene and protein in uterine tissues of primary dysmenorrhea rats decreased significantly( P < 0. 05) after treatment with phloroglucinol combined with exercise. Conclusion: Phloroglucinol combined with exercise can antagonize experimental dysmenorrhea induced by oxytocin in rats.
引文
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[17]高飞,赵洪朋.运动表象训练生理机制相关研究进展[J].解剖科学进展,2014,20(4):375-377.
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[19]徐琳,李海建.科学运动对女大学生月经期情绪因素影响的调查与研究[J].中国科技信息,2009,21(10):229-231.
[2]李玲.2632名职业学院女学生痛经影响因素分析[J].中国妇幼保健,2009,24(26):3693-3694.
[3]孙玉阳,纪宏宇,陈博,等.原发性痛经的发病机制及中医药治疗的研究进展[J].中国药师,2017,20(1):144-147.
[4]范郁山,苗芙蕊,廖爱妮,等.隔药灸“命门”穴对痛经模型大鼠血清雌激素、孕激素水平及其受体表达的影响[J].针刺研究,2013,38(5):352-357.
[5]鲁宁,李立民.间苯三酚治疗原发性痛经的疗效观察[J].医学临床研究,2006,23(2):230-231.
[6]黄昀,矫玮.不同运动方式对青春期原发性痛经症状及子宫血流的影响[J].北京体育大学学报,2007,30(4):495-497.
[7]DISANZO B L,YOU T.Effects of exercise training on indicators of adipose tissue angiogenesis and hypoxia in obese rats[J].Metabolism,2014,63(4):452-455.
[8]王运来,胡虒尧,方庆,等.基于生物网络的当归芍药散、桂枝茯苓丸治疗原发性痛经作用机制研究[J].中药材,2015,38(11):2348-2352.
[9]何瑶,王丽娟,刘婷婷,等.基于代谢组学技术分析四物汤治疗原发性痛经证的作用机制[J].中国实验方剂学杂志,2017,23(12):82-89.
[10]张越,郭琳琳,李哲,等.miR-21对人心肌成纤维细胞MMP2表达和大鼠心肌细胞凋亡的影响[J].解剖科学进展,2015,21(5):460-462+467.
[11]AZIMA S,BAKHSHAYESH H R,KAVIANI M,et al.Comparison of the effect of massage therapy and isometric exercises on primary dysmenorrhea:A randomized controlled clinical trial[J].J Pediatr Adolesc Gynecol,2015,28(6):486-491.
[12]KANNAN P,CLAYDON L S,MILLER D,et al.Vigorous exercises in the management of primary dysmenorrhea:a feasibility study[J].Disabil Rehabil,2015,37(15):1334-1339.
[13]LIU C,LU Q,QU H,et al.Different dosages of mifepristone versus enantone to treat uterine fibroids:A multicenter randomized controlled trial[J].Medicine(Baltimore),2017,96(7):e6124.
[14]唐敏,吴启坤.间苯三酚治疗原发性痛经的临床观察[J].药物流行病学杂志,2006,15(3):150-151.
[15]ARMOUR M,DAHLEN H G,ZHU X,et al.The role of treatment timing and mode of stimulation in the treatment of primary dysmenorrhea with acupuncture:An exploratory randomised controlled trial[J].PLo S One,2017,12(7):e0180177.
[16]ORTIZ M I,CORTES-MARQUEZ S K,ROMERO-QUEZADAL C,et al.Effect of a physiotherapy program in women with primary dysmenorrhea[J].Eur J Obstet Gynecol Reprod Biol,2015,194:24-29.
[17]高飞,赵洪朋.运动表象训练生理机制相关研究进展[J].解剖科学进展,2014,20(4):375-377.
[18]徐拥军,沈聪玲.间苯三酚联合益母草制剂对青少年痛经患者前列腺素E2水平、前列腺素F2α水平、子宫微循环变化指标及临床疗效的影响.中国生化药物杂志,2016.36(1):65-67.
[19]徐琳,李海建.科学运动对女大学生月经期情绪因素影响的调查与研究[J].中国科技信息,2009,21(10):229-231.
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