肺癌组织中P65、CYFRA21-1、vimentin蛋白的表达情况及与临床特征的关系
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摘要
目的探究肺癌组织中P65、细胞角质蛋白19片段抗原21-1(CYFRA21-1)、波形蛋白(vimentin)的表达情况及与肺癌患者临床特征的关系。方法选取60例肺癌患者的肺癌组织及对应的癌旁组织,采用免疫组织化学法检测肺癌组织和癌旁组织中P65、CYFRA21-1、vimentin蛋白的表达情况,并对其与肺癌患者临床特征的关系进行分析。结果肺癌组织中P65、CYFRA21-1、vimentin蛋白的阳性表达率均明显高于癌旁组织(P﹤0.01);临床分期为Ⅲ～Ⅳ期肺癌患者肺癌组织中P65、CYFRA21-1、vimentin蛋白的阳性表达率均明显高于临床分期为Ⅰ～Ⅱ期的患者(P﹤0.01)。有淋巴结转移的肺癌患者肺癌组织中P65和vimentin蛋白的阳性表达率均高于无淋巴结转移的患者(P﹤0.05);不同淋巴结转移情况肺癌患者肺癌组织中CYFRA21-1蛋白的阳性表达率比较,差异无统计学意义(P﹥0.05)。结论肺癌患者肺癌组织中P65、CYFRA21-1、vimentin蛋白的阳性表达率较癌旁组织增高,P65、CYFRA21-1、vimentin蛋白的表达与肺癌患者的临床分期和淋巴结转移情况有一定关联。
Objective To study the expression of P65,cyto-keratin 19 fragment antigen 21-1(CYFRA21-1) and vimentin proteins in lung cancer tissues and to investigate the association with the clinical characteristics of lung cancer.Method A total of 60 cases of lung cancer were included in the study,the lung cancer tissues as well as the adjacent tissues of these subjects were analyzed using immunohistochemistry to determine the expression rate of P65,CYFRA21-1,and vimentin proteins.Besides,the association between expression of P65,CYFRA21-1,vimentin proteins and the clinical characteristics of lung cancer patients was analyzed.Result The positive expression rate of P65,CYFRA21-1,and vimentin proteins in lung cancer tissues were significantly higher than those in adjacent tissues(P<0.01);furthermore,lung cancer patients with clinical stage of III-IV had significantly higher positive expression rate of P65,CYFRA21-1 and vimentin proteins than those with clinical stage of I-II(P<0.01).Presence of lymph node metastasis was associated with higher expression of P65 and vimentin compared with no lymph node metastasis(P<0.05);and no evident difference regarding the CYFRA21-1 protein expression was noted between patients with dissimilar lymph node metastases(P>0.05).Conclusion The positive expression rate of P65,CYFRA21-1,and vimentin protein are higher in lung cancer tissues than those in adjacent tissues,and are sensibly related to the clinical stage and lymph node metastasis of lung cancer.
Objective To study the expression of P65,cyto-keratin 19 fragment antigen 21-1(CYFRA21-1) and vimentin proteins in lung cancer tissues and to investigate the association with the clinical characteristics of lung cancer.Method A total of 60 cases of lung cancer were included in the study,the lung cancer tissues as well as the adjacent tissues of these subjects were analyzed using immunohistochemistry to determine the expression rate of P65,CYFRA21-1,and vimentin proteins.Besides,the association between expression of P65,CYFRA21-1,vimentin proteins and the clinical characteristics of lung cancer patients was analyzed.Result The positive expression rate of P65,CYFRA21-1,and vimentin proteins in lung cancer tissues were significantly higher than those in adjacent tissues(P<0.01);furthermore,lung cancer patients with clinical stage of III-IV had significantly higher positive expression rate of P65,CYFRA21-1 and vimentin proteins than those with clinical stage of I-II(P<0.01).Presence of lymph node metastasis was associated with higher expression of P65 and vimentin compared with no lymph node metastasis(P<0.05);and no evident difference regarding the CYFRA21-1 protein expression was noted between patients with dissimilar lymph node metastases(P>0.05).Conclusion The positive expression rate of P65,CYFRA21-1,and vimentin protein are higher in lung cancer tissues than those in adjacent tissues,and are sensibly related to the clinical stage and lymph node metastasis of lung cancer.
引文
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[12]李世武,陈瑶,虞丽丽,等.波形蛋白表达对非小细胞肺癌患者预后影响的Meta分析[J].中国新药与临床杂志,2015,34(6):441-448.
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[14]段新春,崔永,龚民,等.手术前后血清CEA和CYFRA21-1水平的变化有助于预测非小细胞肺癌患者的预后[J].中国肺癌杂志,2015,18(6):358-364.
[15]周海英,吴爱萍,董洪超,等.E-钙黏蛋白和波形蛋白在非小细胞肺癌中的表达及意义[J].中华实验外科杂志,2017,34(7):1216-1219.
[2]高海祥,邢荣芹,许金伟,等.AKT信号通路在克唑替尼诱导的EML4-ALK阳性肺癌细胞凋亡迁移中的作用及机制研究[J].癌症进展,2017,15(2):132-135.
[3]张晓晔,曲耘,吴荣.NF-κB/p65 siRNA对小鼠Lewis肺癌移植瘤细胞凋亡和Bax表达的影响[J].中国医科大学学报,2017,46(2):145-148.
[4]徐婧玥,刘喆,张贺平,等.肺癌患者血清HE4、CEA、CYFRA21-1水平变化及意义[J].山东医药,2015,55(11):35-36.
[5]邵吕,高东萍,邵燕燕,等.E-钙黏蛋白和波形蛋白表达与非小细胞肺癌铂类药物疗效相关性研究[J].中国现代应用药学,2016,33(12):1592-1597.
[6]叶波,杨龙海,刘向阳.最新国际肺癌TNM分期标准(第7版)修订稿解读[J].中国医刊,2008,18(1):21-23.
[7]项保利,李琳琳,曹亮,等.肺癌患者中转录因子Oct4和Sox2的表达及与肺癌病理分级和临床分期的相关性[J].湖南师范大学学报(医学版),2016,13(1):95-97.
[8]Tiu AC,Potdar R,Djibo DA,et al.Clinical outcomes of African American patients with advanced or metastatic nonsmall cell lung cancer on nivolumab in a single communitybased cancer center[J].Med Oncol,2018,35(7):109.
[9]黄艳,吴春婷,郭红娟,等.人核因子κBp65核定位信号缺失突变对A549细胞恶性表型的影响[J].中国呼吸与危重监护杂志,2015,14(1):52-59.
[10]李龙飞,渠敬明,王祥军,等.不同类型肺癌患者化疗前后血清CEA、NSE及CYFRA21-1的表达及意义[J].中国老年学杂志,2018,38(4):821-823.
[11]谢文君,朱莉艳,虞小芳,等.肺癌患者肺部感染的病原菌分布及血清中NSE和CYFRA21-1病理表达的临床研究[J].中华医院感染学杂志,2018,28(7):1008-1011.
[12]李世武,陈瑶,虞丽丽,等.波形蛋白表达对非小细胞肺癌患者预后影响的Meta分析[J].中国新药与临床杂志,2015,34(6):441-448.
[13]李宏宇,李沛,蔡三立,等.血清CEA、NSE、CYFRA21-1在肺癌诊治及预后判断中的临床价值[J].中国老年学杂志,2015,35(22):6458-6459.
[14]段新春,崔永,龚民,等.手术前后血清CEA和CYFRA21-1水平的变化有助于预测非小细胞肺癌患者的预后[J].中国肺癌杂志,2015,18(6):358-364.
[15]周海英,吴爱萍,董洪超,等.E-钙黏蛋白和波形蛋白在非小细胞肺癌中的表达及意义[J].中华实验外科杂志,2017,34(7):1216-1219.
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