不同甘薯品种抗UV-B辐射增强的效应分析
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摘要
用比色法研究人工增强UV-B辐射对盆栽‘滇紫甘薯24’(‘DZS24’)和‘徽薯’(‘HS’)成熟叶片渗透调节物质含量和抗氧化酶比活力的影响及其种间差异。结果表明:增强UV-B辐射下2品种的可溶性糖含量(SSC)均随辐射强度的增加而显著降低,可溶性蛋白质含量(SPC)均为前期随辐射强度的增加而降低,后期随辐射强度的增加而升高,但‘HS’的变化幅度均大于‘DZS24’,‘DZS24’的游离脯氨酸含量(FPC)随辐射强度的增加而增加,‘HS’则与其相反;2品种的超氧化物歧化酶(SOD)和过氧化氢酶(CAT)比活力随辐射强度的增加而升高,POD(过氧化物酶)比活力随辐射强度的增加而降低,但相同辐射下‘DZS24’的SOD比活力的增幅大于‘HS’,POD和CAT比活力的变幅小于‘HS’。因此‘DZS24’具有比‘HS’更强的渗透调节和抗氧化能力,更适于在UV-B辐射强烈的低纬高原地区种植。
The effects of artificial UV-B enhancement on osmoregulation substance contents and specific activity of the antioxidant enzymes and their interspecific differences of the mature leaves of potted ‘Dianziganshu24'(‘DZS24')and ‘Huishu'(‘HS') were studied by using colorimetry. The results showed that: under enhanced UV-B, the soluble sugar contents(SSC) significantly reduced with the UV-B enhancement, the soluble protein contents(SPC)decreased at the earlier stage and increased at the later stage with the enhancement for two cultivars. Moreover, the variation range of SSC and SPC of ‘HS' was larger than that in ‘DZS24'. The free proline contents(FPC) increased in ‘DZS24' and decreased in ‘HS' with the enhancement. The specific activity of superoxide dismutase(SOD) and catalase(CAT) increased, while the peroxidase(POD) specific activity decreased with the enhancement for the two cultivars. However, the increasing range of SOD specific activity of ‘DZS24' was larger than ‘HS' and the variation range of POD and CAT specific activity was less than ‘HS' under the same UV-B. Therefore the osmotic regulation and antioxidant capacity of ‘DZS24' is stronger than ‘HS' and more suitable for planting in low latitude plateau with the intense UV-B.
The effects of artificial UV-B enhancement on osmoregulation substance contents and specific activity of the antioxidant enzymes and their interspecific differences of the mature leaves of potted ‘Dianziganshu24'(‘DZS24')and ‘Huishu'(‘HS') were studied by using colorimetry. The results showed that: under enhanced UV-B, the soluble sugar contents(SSC) significantly reduced with the UV-B enhancement, the soluble protein contents(SPC)decreased at the earlier stage and increased at the later stage with the enhancement for two cultivars. Moreover, the variation range of SSC and SPC of ‘HS' was larger than that in ‘DZS24'. The free proline contents(FPC) increased in ‘DZS24' and decreased in ‘HS' with the enhancement. The specific activity of superoxide dismutase(SOD) and catalase(CAT) increased, while the peroxidase(POD) specific activity decreased with the enhancement for the two cultivars. However, the increasing range of SOD specific activity of ‘DZS24' was larger than ‘HS' and the variation range of POD and CAT specific activity was less than ‘HS' under the same UV-B. Therefore the osmotic regulation and antioxidant capacity of ‘DZS24' is stronger than ‘HS' and more suitable for planting in low latitude plateau with the intense UV-B.
引文
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[10]彭强,高彦祥,袁芳.紫甘薯及其花色苷的研究与开发进展[J].食品科学,2010,31(23):401-405.Peng Q,Gao Y X,Yuan F.Research progress on purple sweet potato and its anthocyanins[J].Food Science,2010,31(23):401-405.(in Chinese)
[11]吴春,孙胜敏,姜黎明,等.紫甘薯花青素的功能特性研究[J].化学与黏合,2012,34(6):13-14.Wu C,Sun S M,Jiang L M,et al.Study on functional properties of purple sweet potato anthocyanins[J].Chemistry and Adhesion,2012,34(6):13-14.(in Chinese)
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[13]吴杏春,林文雄,郭玉春,等.UV-B辐射增强对水稻叶片抗氧化系统的影响[J].福建农业学报,2001,16(3):51-55.Wu X C,Lin W X,Guo Y C,et al.Effect of enhancing Ultraviolet-B radiation on antioxidant systems in rice seedling leaves[J].Fujian Journal of Agricultural Sciences,2001,16(3):51-55.(in Chinese)
[14]林文雄.水稻对UV-B辐射增强的生理响应及其分子机制研究[J].中国生态农业学报,2013,21(1):119-126.Lin W X.Physiological responses and molecular mechanism of rice(Oryza sativa)exposed to enhanced UV-B radiation[J].Chinese Journal of Eco-Agriculture,2013,21(1):119-126.(in Chinese)
[15]龚燕雄,张贵合,李彩斌,等.紫甘薯新品系在云南不同地区的生长表现及其块根花色苷含量差异[J].云南农业大学学报,2017,32(4):582-587.Gong Y X,Zhang G H,Li C B,et al.Growth performance and content of anthocyanin in new purple sweet potato lines in different regions of Yunnan[J].Journal of Yunnan Agricultural University,2017,32(4):582-587.(in Chinese)
[16]孙茂林.云南薯类作物的研究和发展[M].昆明:云南科技出版社,2003:193-194,197.Sun M L.Research and development of potato crops in Yunnan[M].Kunming:Yunnan Science and Technology Press,2003:193-194,197.(in Chinese)
[17]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:142-143,144-147,228-230.Gao J F.Experimental instruction in plant physiology[M].Beijing:Higher Education Press,2006:142-143,144-147,228-230.(in Chinese)
[18]杨鑫,李丽淑,樊吴静,等.诱抗剂对马铃薯疮痂病抗性诱导的生理机制[J].南方农业学报,2018,49(6):1111-1117.Yang X,Li L S,Fan W J,et al.Physiological mechanism of inducers to resistance inducing of potato scab[J].Journal of Southern Agriculture,2018,49(6):1111-1117.(in Chinese)
[19]郝文芳,赵洁,蔡彩虹,等.3种胡枝子抗氧化酶和渗透调节物质对干旱和增强UV-B辐射的动态响应[J].环境科学学报,2013,33(8):2349-2358.Hao W F,Zhao J,Cai C H,et al.Dynamic response of antioxidant enzymes and osmotic adjustment substances to drought and enhanced UV-B radiation in three Lespedezas[J].Acta Scientiae Circumstantiae,2013,33(8):2349-2358.(in Chinese)
[20]杨璐,赵天宏.UV-B辐射增强对大豆根系活性氧代谢及抗氧化系统的影响[J].华北农学报,2018,33(5):174-180.Yang L,Zhao T H.The effect of enhanced UV-B radiation on soybean root's activate oxygen metabolism and antioxidative system[J].Acta Agriculturae Boreali-Sinica,2018,33(5):174-180.(in Chinese)
[21]邹凯茜,商桑,田丽波,等.低温胁迫对嫁接苦瓜幼苗渗透调节物质的影响[J].热带作物学报,2018,39(8):1533-1539.Zou K X,Shang S,Tian L B,et al.Effects of low temperature stress on osmotic solutes of grafted bitter gourd seedlings[J].Chinese Journal of Tropical Crops,2018,39(8):1533-1539.(in Chinese)
[22]Nedunchezhian N,Annamalainathan K,Kulandaivelu G.Induction of heat shock-like proteins in Vigna sinensis seedlings growing under ultraviolet-B(280-320nm)enhanced radiation[J].Physiologia Plantarum,2010,85(3):503-506.
[23]董靖,李红丽,董智,等.H2S对NaCl胁迫下草木樨幼苗生理指标及抗氧化酶活性的影响[J].草业科学,2018,35(10):2430-2437.Dong J,Li H L,Dong Z,et al.Effect of H2S on physiological indexes and antioxidant activity of sweet clover seedlings under NaCl stress[J].Pratacultural Science,2018,35(10):2430-2437.(in Chinese)
[24]Santos I,Fidalgo F,Almeida J M,et al.Biochemical and ultrastructural changes in leaves of potato plants grown under supplementary UV-B radiation[J].Plant Science,2004,167(4):925-935.
[25]陈宗瑜,王毅,钟楚.自然和人为条件下UV-B辐射减弱对烟草抗氧化酶活性的影响[J].中国农业气象,2010,31(3):395-401.Chen Z Y,Wang Y,Zhong C.Effects of solar UV-B radiation on antioxidase activity in tobacco leaf[J].Chinese Journal of Agrometeorology,2010,31(3):395-401.(in Chinese)
[26]李惠梅,师生波.增强UV-B辐射对麻花艽叶片的抗氧化酶的影响[J].西北植物学报,2005,25(3):519-524.Li H M,Shi S B.Effects of enhanced UV-B radiation on antioxydative enzymes in Gentiana straminea leaves[J].Acta Botanica Boreali-Occidentalia Sinica,2005,25(3):519-524.(in Chinese)
[27]战莘晔,殷红,李雪莹,等.UV-B辐射增强对粳稻光合特性及保护酶活性的影响[J].沈阳农业大学学报,2014,45(5):513-517.Zhan S Y,Yin H,Li X Y,et al.Effect of UV-B radiation on photosynthetic characteristics and protective enzyme activities of Japonica rice[J].Journal of Shenyang Agricultural University,2014,45(5):513-517.(in Chinese)
[2]张海丽.过量表达Lsi1增强水稻抗UV-B辐射机制的初步研究[D].福州:福建农林大学,2009:1.Zhang H L.Preliminary study on the mechanism of overexpression low silicon rice gene 1(Lsi1)to enhance rice UV-B resistance[D].Fuzhou:Fujian Agriculture and Forestry University,2009:1.(in Chinese)
[3]Brown B A,Jenkins G I.UV-B signaling pathways with different fluence-rate response profiles are distinguished in mature Arabidopsis leaf tissue by requirement for UVR8,HY5,and HYH[J].Plant Physiology,2008,146(2):576-88.
[4]Mao C X,Chen M M,Wang L,et al.Protective effect of cerium ion against ultraviolet B radiation-induced water stress in soybean seedlings[J].Biological Trace Element Research,2012,146(3):381-387.
[5]Ning W,Peng X,Ma L,et al.Enhanced secondary metabolites production and antioxidant activity in postharvest Lonicera japonica Thunb.in response to UV radiation[J].Innov Food Sci&Emerg Techn,2012,13(1):231-243.
[6]陈贵,胡文玉,谢甫绨,等.提取植物体内MDA的溶剂及MDA作为衰老指标的探讨[J].植物生理学通讯,1991,27(1):44-46.Chen G,Hu W Y,Xie P T,et al.Solvent for extracting malondialdehyde in plant as an index of senescence[J].Plant Physiology Journal,1991,27(1):44-46.(in Chinese)
[7]苏贝贝.UV-B辐射对颠茄生理特性及次生代谢产物含量的影响[D].重庆:西南大学,2016:27.Su B B.Effects of UV-B on physiological characteristics and secondary metabolites content of the Atropa belladonna L.[D].Chongqing:Southwest University,2016:27.(in Chinese)
[8]李晓阳,陈慧泽,韩榕.UV-B辐射对拟南芥种子萌发和幼苗生长的影响[J].植物学报,2013,48(1):52-58.Li X Y,Chen H Z,Han R.Effect of UV-B irradiation on seed germination and seedling growth of Arabidopsis[J].Chinese Bulletin of Botany,2013,48(1):52-58.(in Chinese)
[9]欧阳磊,周冀衡,陈习羽,等.UV-B辐射对烟草形态、光合色素和抗氧化酶活性的影响[J].湖南农业科学,2012,(5):21-23.Ouyang L,Zhou J H,Chen X Y,et al.Effects of UV-B radiation on tobacco morphology and photosynthetic pigments and activity of antioxidant enzyme in tobacco[J].Hunan Agricultural Sciences,2012,(5):21-23.(in Chinese)
[10]彭强,高彦祥,袁芳.紫甘薯及其花色苷的研究与开发进展[J].食品科学,2010,31(23):401-405.Peng Q,Gao Y X,Yuan F.Research progress on purple sweet potato and its anthocyanins[J].Food Science,2010,31(23):401-405.(in Chinese)
[11]吴春,孙胜敏,姜黎明,等.紫甘薯花青素的功能特性研究[J].化学与黏合,2012,34(6):13-14.Wu C,Sun S M,Jiang L M,et al.Study on functional properties of purple sweet potato anthocyanins[J].Chemistry and Adhesion,2012,34(6):13-14.(in Chinese)
[12]陈宗瑜.云南气候总论[M].北京:气象出版社,2001:47.Chen Z Y.General discussion of Yunnan climate[M].Beijing:China Meteorological Press,2001:47.(in Chinese)
[13]吴杏春,林文雄,郭玉春,等.UV-B辐射增强对水稻叶片抗氧化系统的影响[J].福建农业学报,2001,16(3):51-55.Wu X C,Lin W X,Guo Y C,et al.Effect of enhancing Ultraviolet-B radiation on antioxidant systems in rice seedling leaves[J].Fujian Journal of Agricultural Sciences,2001,16(3):51-55.(in Chinese)
[14]林文雄.水稻对UV-B辐射增强的生理响应及其分子机制研究[J].中国生态农业学报,2013,21(1):119-126.Lin W X.Physiological responses and molecular mechanism of rice(Oryza sativa)exposed to enhanced UV-B radiation[J].Chinese Journal of Eco-Agriculture,2013,21(1):119-126.(in Chinese)
[15]龚燕雄,张贵合,李彩斌,等.紫甘薯新品系在云南不同地区的生长表现及其块根花色苷含量差异[J].云南农业大学学报,2017,32(4):582-587.Gong Y X,Zhang G H,Li C B,et al.Growth performance and content of anthocyanin in new purple sweet potato lines in different regions of Yunnan[J].Journal of Yunnan Agricultural University,2017,32(4):582-587.(in Chinese)
[16]孙茂林.云南薯类作物的研究和发展[M].昆明:云南科技出版社,2003:193-194,197.Sun M L.Research and development of potato crops in Yunnan[M].Kunming:Yunnan Science and Technology Press,2003:193-194,197.(in Chinese)
[17]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:142-143,144-147,228-230.Gao J F.Experimental instruction in plant physiology[M].Beijing:Higher Education Press,2006:142-143,144-147,228-230.(in Chinese)
[18]杨鑫,李丽淑,樊吴静,等.诱抗剂对马铃薯疮痂病抗性诱导的生理机制[J].南方农业学报,2018,49(6):1111-1117.Yang X,Li L S,Fan W J,et al.Physiological mechanism of inducers to resistance inducing of potato scab[J].Journal of Southern Agriculture,2018,49(6):1111-1117.(in Chinese)
[19]郝文芳,赵洁,蔡彩虹,等.3种胡枝子抗氧化酶和渗透调节物质对干旱和增强UV-B辐射的动态响应[J].环境科学学报,2013,33(8):2349-2358.Hao W F,Zhao J,Cai C H,et al.Dynamic response of antioxidant enzymes and osmotic adjustment substances to drought and enhanced UV-B radiation in three Lespedezas[J].Acta Scientiae Circumstantiae,2013,33(8):2349-2358.(in Chinese)
[20]杨璐,赵天宏.UV-B辐射增强对大豆根系活性氧代谢及抗氧化系统的影响[J].华北农学报,2018,33(5):174-180.Yang L,Zhao T H.The effect of enhanced UV-B radiation on soybean root's activate oxygen metabolism and antioxidative system[J].Acta Agriculturae Boreali-Sinica,2018,33(5):174-180.(in Chinese)
[21]邹凯茜,商桑,田丽波,等.低温胁迫对嫁接苦瓜幼苗渗透调节物质的影响[J].热带作物学报,2018,39(8):1533-1539.Zou K X,Shang S,Tian L B,et al.Effects of low temperature stress on osmotic solutes of grafted bitter gourd seedlings[J].Chinese Journal of Tropical Crops,2018,39(8):1533-1539.(in Chinese)
[22]Nedunchezhian N,Annamalainathan K,Kulandaivelu G.Induction of heat shock-like proteins in Vigna sinensis seedlings growing under ultraviolet-B(280-320nm)enhanced radiation[J].Physiologia Plantarum,2010,85(3):503-506.
[23]董靖,李红丽,董智,等.H2S对NaCl胁迫下草木樨幼苗生理指标及抗氧化酶活性的影响[J].草业科学,2018,35(10):2430-2437.Dong J,Li H L,Dong Z,et al.Effect of H2S on physiological indexes and antioxidant activity of sweet clover seedlings under NaCl stress[J].Pratacultural Science,2018,35(10):2430-2437.(in Chinese)
[24]Santos I,Fidalgo F,Almeida J M,et al.Biochemical and ultrastructural changes in leaves of potato plants grown under supplementary UV-B radiation[J].Plant Science,2004,167(4):925-935.
[25]陈宗瑜,王毅,钟楚.自然和人为条件下UV-B辐射减弱对烟草抗氧化酶活性的影响[J].中国农业气象,2010,31(3):395-401.Chen Z Y,Wang Y,Zhong C.Effects of solar UV-B radiation on antioxidase activity in tobacco leaf[J].Chinese Journal of Agrometeorology,2010,31(3):395-401.(in Chinese)
[26]李惠梅,师生波.增强UV-B辐射对麻花艽叶片的抗氧化酶的影响[J].西北植物学报,2005,25(3):519-524.Li H M,Shi S B.Effects of enhanced UV-B radiation on antioxydative enzymes in Gentiana straminea leaves[J].Acta Botanica Boreali-Occidentalia Sinica,2005,25(3):519-524.(in Chinese)
[27]战莘晔,殷红,李雪莹,等.UV-B辐射增强对粳稻光合特性及保护酶活性的影响[J].沈阳农业大学学报,2014,45(5):513-517.Zhan S Y,Yin H,Li X Y,et al.Effect of UV-B radiation on photosynthetic characteristics and protective enzyme activities of Japonica rice[J].Journal of Shenyang Agricultural University,2014,45(5):513-517.(in Chinese)