生长基质和激素对麻楝嫩枝扦插生根的影响
|
摘要
以麻楝当年生嫩枝为试验材料,开展了扦插基质,激素种类、浓度和处理时间对麻楝嫩枝扦插生根影响的研究。基质试验采用泥炭土、黄心土、河沙、蛭石为扦插基质,完全随机区组试验设计;激素试验采用激素种类(NAA、IBA、ABT1)、浓度(500、1 000、1 500 mg·L~(-1))及处理时间(10 s、1 min、30 min) 3因素3水平正交(L_9(3~3))试验设计。结果表明:(1)泥炭土+蛭石(1∶1,体积比)为最佳组合基质,其生根率达81. 81%,平均生根数量为20. 33条,平均根长为4. 80 cm,根系效果指数达3. 05;(2)激素种类对麻楝嫩枝扦插生根效果影响最大,激素浓度次之,处理时间影响最小。扦插时,采用泥炭土+蛭石(1∶1)混合基质、1 000 mg·L~(-1)浓度的ABT1号生根粉溶液、浸泡插穗10 s的生根效果最佳。
We studiedthe effects of substrate,hormonetype,concentration and treating time on cutting rooting of Chukrasia tabularis with young shoots as test materials. The substrate treatments include peat soil,yellowsoil,river sand and vermiculite,adopting with a randomized complete block design. The hormone treatments adopted L_9 (3~3) orthogonal design with different hormone kinds (NAA,IBA and ABT),concentrations (500,1 000 and 1 500 mg · L~(-1)),and treating time (10 s,1 min and 30 min). The results showed that: (1) The mixed substrate of peat soil + vermiculite (1 ∶1,volume ratio) was the best,with the rooting rate of 81. 81%,mean rooting number of 20. 33 per plant,the average root length of 4. 80 cm,and the root effective index of 3. 05; (2) The hormone kinds had the greatest influence on cutting effectiveness of C. tabularis young shoots among the three main factors,followed by hormone concentrations,and the treating time had the least influence. In cutting,under the mixed substrate of peat soil + vermiculite (1∶1),treated ewith ABT1 of 1 000 mg·L~(-1) for 10 s,the rooting effective was the best.
We studiedthe effects of substrate,hormonetype,concentration and treating time on cutting rooting of Chukrasia tabularis with young shoots as test materials. The substrate treatments include peat soil,yellowsoil,river sand and vermiculite,adopting with a randomized complete block design. The hormone treatments adopted L_9 (3~3) orthogonal design with different hormone kinds (NAA,IBA and ABT),concentrations (500,1 000 and 1 500 mg · L~(-1)),and treating time (10 s,1 min and 30 min). The results showed that: (1) The mixed substrate of peat soil + vermiculite (1 ∶1,volume ratio) was the best,with the rooting rate of 81. 81%,mean rooting number of 20. 33 per plant,the average root length of 4. 80 cm,and the root effective index of 3. 05; (2) The hormone kinds had the greatest influence on cutting effectiveness of C. tabularis young shoots among the three main factors,followed by hormone concentrations,and the treating time had the least influence. In cutting,under the mixed substrate of peat soil + vermiculite (1∶1),treated ewith ABT1 of 1 000 mg·L~(-1) for 10 s,the rooting effective was the best.
引文
1.Kalinganire A,Pinyopusarerk K.Chukrasia:biology,cultivation and utilisation[R].Canberra:ACIAR,2000:35.
2.仲崇禄,洪长福,白嘉雨,等.麻楝属树种种源苗期试验及其在我国发展潜力[J].广东林业科技,2001,17(4):26-31.Zhong C L,Hong C F,Bai J Y,et al.An experiment on 10provenances of Chukrasia seedlings and their analysis on development potential in southern China[J].Forestry Science and Technology of Guangdong Province,2001,17(4):26-31.
3.鲍泥满.单穗升麻和麻楝的化学成分及其生物活性研究[D].昆明:云南大学,2015.Bao N M.Studies on the chemical constituents and bioactivity of Cimicifuga simplex WORMSK and Chukrasia tabularis A.Juss.[D].Kunming:Yunnan University,2015.
4.Yi L,Zhang H J,Tian X M,et al.Four new limonoids from the seeds of Chukrasia tabularis A.Juss.[J].Phytochemistry Letters,2017,19:12-17.
5.彭俊霖,梅文莉,刘子琦,等.麻楝枝干的化学成分及其α-葡萄糖苷酶抑制活性研究[J].热带亚热带植物学报,2016,24(1):99-105.Peng J L,Mei W L,Liu Z Q,et al.Studies on the chemical constituents from the stems of Chukrasia tabularis and theirα-Glucosidase inhibitory activity[J].Journal of Tropical and Subtropical Botany,2016,24(1):99-105.
6.Zhang C R,Yang S P,Chen X Q,et al.Limonoids from the twigs and leaves of Chukrasia tabularis[J].Helvetica Chimica Acta,2008,91(12):2338-2350.
7.罗志明,李儒法.麻楝地理种源苗期试验研究[J].热带林业,2007,35(3):28-30.Luo Z M,LI R F.Experimental study on the seedling stage of the geographic provenance of Chukrasia tabularis[J].Tropical Forestry,2007,35(3):28-30.
8.武冲,张勇,仲崇禄.麻楝种子育苗技术[J].林业实用技术,2011,(12):34.Wu C,Zhang Y,Zhong C L.Seed breeding technologyof Chukrasia tabularis[J].Practical Forestry Technology,2011,(12):34.
9.马赛宇,耿云芬.麻楝容器育苗技术[J].林业科技通讯,2015(4):28-29.Ma S Y,Geng Y F.Container seedling technologyof Chukrasia tabularis[J].Forest Science and Technology,2015(4):28-29.
10.武冲.麻楝种质资源遗传多样性研究[D].北京:中国林业科学研究院,2013.Wu C.Genetic diversity of germplasmresources of Chukrasia tabularis A.Juss.[D].Beijing:Chinese Academy of Forestry,2013.
11.武冲,仲崇禄,张勇,等.麻楝ISSR-PCR反应体系的建立及优化[J].浙江农林大学学报,2013,30(4):620-626.Wu C,Zhong C L,Zhang Y,et al.Establishment and optimization of an ISSR-PCR systemfor Chukrasia tabularis[J].Journal of Zhejiang A&F University,2013,30(4):620-626.
12.Wu C,Zong C L,Zhang Y,et al.Genetic diversity and genetic relationships of Chukrasia spp.(Meliaceae)as revealed by inter simple sequence repeat(ISSR)markers[J].Trees,2014,28(6):1847-1857.
13.刘斌,薛立,许鹏波,等.短期低温下稀土对麻楝幼苗生理指标的影响[J].华南农业大学学报,2010,31(4):82-85.Liu B,Xue L,Xu P B,et al.Effectsof rare earthon physiological indicesof Chukrasia tabularis seedlings under shorttime low temperature[J].Journalof South China Agricultural University,2010,31(4):82-85.
14.许鹏波,薛立,潘澜,等.稀土对低温胁迫麻楝幼苗生理生化特性的影响[J].中南林业科技大学学报,2011,31(2):34-40.Xu P B,Xue L,Pan L,et al.Effects of rare earth on physiological and biochemical characteristicsin Chukrasia tabularis seedlings under low-temperature stress[J].Journal of Central South University of Forestry&Technology,2011,31(2):34-40.
15.Bentzer B G.Rooting and early shoot characteristics of Piceaabies(L.)karst.cuttings originating from shoots with enforced vertical growth[J].Scandinavian Journal of Forest Research,1988,3(1-4):481-491.
16.丁丽萍,马力,占玉芳,等.基于主成分分析的不同激素处理下宁夏枸杞扦插生根效果的综合评价[J].经济林研究,2017,35(2):110-115.Ding L P,Ma L,Zhan Y F,et al.Comprehensive evaluation of rooting effect of different hormone treatments on Lyciumbarbarum cuttings based on principal component analysis[J].Nonwood Forest Research,2017,35(2):110-115.
17.杨维忠,张甜.SPSS统计分析与行业应用案例详解[M].北京:清华大学出版社,2011.Yang W Z,Zhang T.SPSS statistical analysis and industry application cases[M].Beijing:Tsinghua University Press,2011.
18.王东光,尹光天,邹文涛,等.不同基质和季节对闽楠嫩枝扦插生根的影响[J].热带作物学报,2013,34(8):1458-1462.Wang D G,Yin G T,Zou W T,et al.Effects of different subsrates and seasons on shoot cutting propagation of Phoebe bournei[J].Chinese Journal of Tropical Crops,2013,34(8):1458-1462.
19.赵翔.灰木莲扦插和嫁接繁殖技术的研究[D].哈尔滨:东北林业大学,2017.Zhao X.Research on the cutting and grafting propagation of Manglietiaconifera dandy[D].Harbin:Northeast Forestry University,2017.
20.陈庆生,周鹏,张敏,等.激素和插穗处理对乌饭树扦插生根的影响[J].东北林业大学学报,2016,44(4):41-43,47.Chen Q S,Zhou P,Zhang M,et al.Influence of different treatment of cuttings and hormone on rooting of cutting of Vaccinium bracteatum[J].Journal of Northeast Forestry U-niversity,2016,44(4):41-43,47.
21.胡勐鸿,欧阳芳群,贾子瑞,等.欧洲云杉扦插生根影响因子研究与生根力优良单株选择[J].林业科学,2014,50(2):42-49.Hu M H,Ouyang Q F,Jia Z R,et al.Factors affecting rooting of piceaabies shoot cuttings and individual selection with High rooting ability[J].Scientia Silvae Sinicae,2014,50(2):42-49.
22.张乐华,王书胜,单文,等.基质、激素种类及其浓度对鹿角杜鹃扦插育苗的影响[J].林业科学,2014,50(3):45-54.Zhang L H,Wang S S,Shan W,et al.Influences of growth media,and hormone types and concentrations on cutting propagation of Rhododendron latoucheae[J].Scientia Silvae Sinicae,2014,50(3):45-54.
23.Bartel B,Leclere S,Magidin M,et al.Inputs to the active indole-3-acetic acid pool:Denovo synthesis,conjugate hydrolysis,and indole-3-butyric acidβ-oxidation[J].Journal of Plant Growth Regulation,2001,20(3):198-216.
24.Kochhar S,Singh S P,Kochhar V K.Effect of auxins and associated biochemical changes during clonal propagation of the biofuel plant-Jatrophacurcas[J].Biomass and Bioenergy,2008,32(12):1136-1143.
25.张玉臣,周再知,梁坤南,等.不同植物生长调节剂对白木香扦插生根的影响[J].林业科学研究,2010,23(2):278-282.Zhang Y C,Zhou Z Z,Liang K N,et al.Effectof different plant growth regulators on the rootingof Aquilaria sinensis cuttings[J].Forest Research,2010,23(2):278-282.
26.徐笑玥,佟兆庆,闫文涛,等.中华金叶榆嫩枝扦插繁殖技术[J].东北林业大学学报,2016,44(5):1-4.Xu X Y,Tong Z Q,Yan W T,et al.Soft cutting propagation technology of Ulmus pumila‘Zhonghuajinye’[J].Journal of Northeast Forestry University,2016,44(5):1-4.
27.吕跃东,姚颖,吕跃伟,等.不同激素和基质对黑穗醋栗扦插繁殖的影响[J].经济林研究,2015,33(4):144-147.LüY D,Yao Y,LüY W,et al.Effects of different hormones and media on cutting propagation in black currant[J].Nonwood Forest Research,2015,33(4):144-147.
28.闫海霞,卢家仕,黄昌艳,等.萘乙酸和吲哚丁酸对月季扦插成活效果的影响[J].南方农业学报,2013,44(11):1870-1873.Yan H X,Lu J S,Huang C Y,et al.Effects of NAA and IBA on survival of rosehardwood cutting[J].Journal of Southern Agriculture,2013,44(11):1870-1873.
29.魏树强.杂交鹅掌楸扦插繁殖技术与生根机理研究[D].南京:南京林业大学,2009.Wei S Q.Studies on the cutting propagation technology and rooting mechanism of Liriodendron chinese×L.tulipifera[D].Nanjing:Nanjing Forestry University,2009.
2.仲崇禄,洪长福,白嘉雨,等.麻楝属树种种源苗期试验及其在我国发展潜力[J].广东林业科技,2001,17(4):26-31.Zhong C L,Hong C F,Bai J Y,et al.An experiment on 10provenances of Chukrasia seedlings and their analysis on development potential in southern China[J].Forestry Science and Technology of Guangdong Province,2001,17(4):26-31.
3.鲍泥满.单穗升麻和麻楝的化学成分及其生物活性研究[D].昆明:云南大学,2015.Bao N M.Studies on the chemical constituents and bioactivity of Cimicifuga simplex WORMSK and Chukrasia tabularis A.Juss.[D].Kunming:Yunnan University,2015.
4.Yi L,Zhang H J,Tian X M,et al.Four new limonoids from the seeds of Chukrasia tabularis A.Juss.[J].Phytochemistry Letters,2017,19:12-17.
5.彭俊霖,梅文莉,刘子琦,等.麻楝枝干的化学成分及其α-葡萄糖苷酶抑制活性研究[J].热带亚热带植物学报,2016,24(1):99-105.Peng J L,Mei W L,Liu Z Q,et al.Studies on the chemical constituents from the stems of Chukrasia tabularis and theirα-Glucosidase inhibitory activity[J].Journal of Tropical and Subtropical Botany,2016,24(1):99-105.
6.Zhang C R,Yang S P,Chen X Q,et al.Limonoids from the twigs and leaves of Chukrasia tabularis[J].Helvetica Chimica Acta,2008,91(12):2338-2350.
7.罗志明,李儒法.麻楝地理种源苗期试验研究[J].热带林业,2007,35(3):28-30.Luo Z M,LI R F.Experimental study on the seedling stage of the geographic provenance of Chukrasia tabularis[J].Tropical Forestry,2007,35(3):28-30.
8.武冲,张勇,仲崇禄.麻楝种子育苗技术[J].林业实用技术,2011,(12):34.Wu C,Zhang Y,Zhong C L.Seed breeding technologyof Chukrasia tabularis[J].Practical Forestry Technology,2011,(12):34.
9.马赛宇,耿云芬.麻楝容器育苗技术[J].林业科技通讯,2015(4):28-29.Ma S Y,Geng Y F.Container seedling technologyof Chukrasia tabularis[J].Forest Science and Technology,2015(4):28-29.
10.武冲.麻楝种质资源遗传多样性研究[D].北京:中国林业科学研究院,2013.Wu C.Genetic diversity of germplasmresources of Chukrasia tabularis A.Juss.[D].Beijing:Chinese Academy of Forestry,2013.
11.武冲,仲崇禄,张勇,等.麻楝ISSR-PCR反应体系的建立及优化[J].浙江农林大学学报,2013,30(4):620-626.Wu C,Zhong C L,Zhang Y,et al.Establishment and optimization of an ISSR-PCR systemfor Chukrasia tabularis[J].Journal of Zhejiang A&F University,2013,30(4):620-626.
12.Wu C,Zong C L,Zhang Y,et al.Genetic diversity and genetic relationships of Chukrasia spp.(Meliaceae)as revealed by inter simple sequence repeat(ISSR)markers[J].Trees,2014,28(6):1847-1857.
13.刘斌,薛立,许鹏波,等.短期低温下稀土对麻楝幼苗生理指标的影响[J].华南农业大学学报,2010,31(4):82-85.Liu B,Xue L,Xu P B,et al.Effectsof rare earthon physiological indicesof Chukrasia tabularis seedlings under shorttime low temperature[J].Journalof South China Agricultural University,2010,31(4):82-85.
14.许鹏波,薛立,潘澜,等.稀土对低温胁迫麻楝幼苗生理生化特性的影响[J].中南林业科技大学学报,2011,31(2):34-40.Xu P B,Xue L,Pan L,et al.Effects of rare earth on physiological and biochemical characteristicsin Chukrasia tabularis seedlings under low-temperature stress[J].Journal of Central South University of Forestry&Technology,2011,31(2):34-40.
15.Bentzer B G.Rooting and early shoot characteristics of Piceaabies(L.)karst.cuttings originating from shoots with enforced vertical growth[J].Scandinavian Journal of Forest Research,1988,3(1-4):481-491.
16.丁丽萍,马力,占玉芳,等.基于主成分分析的不同激素处理下宁夏枸杞扦插生根效果的综合评价[J].经济林研究,2017,35(2):110-115.Ding L P,Ma L,Zhan Y F,et al.Comprehensive evaluation of rooting effect of different hormone treatments on Lyciumbarbarum cuttings based on principal component analysis[J].Nonwood Forest Research,2017,35(2):110-115.
17.杨维忠,张甜.SPSS统计分析与行业应用案例详解[M].北京:清华大学出版社,2011.Yang W Z,Zhang T.SPSS statistical analysis and industry application cases[M].Beijing:Tsinghua University Press,2011.
18.王东光,尹光天,邹文涛,等.不同基质和季节对闽楠嫩枝扦插生根的影响[J].热带作物学报,2013,34(8):1458-1462.Wang D G,Yin G T,Zou W T,et al.Effects of different subsrates and seasons on shoot cutting propagation of Phoebe bournei[J].Chinese Journal of Tropical Crops,2013,34(8):1458-1462.
19.赵翔.灰木莲扦插和嫁接繁殖技术的研究[D].哈尔滨:东北林业大学,2017.Zhao X.Research on the cutting and grafting propagation of Manglietiaconifera dandy[D].Harbin:Northeast Forestry University,2017.
20.陈庆生,周鹏,张敏,等.激素和插穗处理对乌饭树扦插生根的影响[J].东北林业大学学报,2016,44(4):41-43,47.Chen Q S,Zhou P,Zhang M,et al.Influence of different treatment of cuttings and hormone on rooting of cutting of Vaccinium bracteatum[J].Journal of Northeast Forestry U-niversity,2016,44(4):41-43,47.
21.胡勐鸿,欧阳芳群,贾子瑞,等.欧洲云杉扦插生根影响因子研究与生根力优良单株选择[J].林业科学,2014,50(2):42-49.Hu M H,Ouyang Q F,Jia Z R,et al.Factors affecting rooting of piceaabies shoot cuttings and individual selection with High rooting ability[J].Scientia Silvae Sinicae,2014,50(2):42-49.
22.张乐华,王书胜,单文,等.基质、激素种类及其浓度对鹿角杜鹃扦插育苗的影响[J].林业科学,2014,50(3):45-54.Zhang L H,Wang S S,Shan W,et al.Influences of growth media,and hormone types and concentrations on cutting propagation of Rhododendron latoucheae[J].Scientia Silvae Sinicae,2014,50(3):45-54.
23.Bartel B,Leclere S,Magidin M,et al.Inputs to the active indole-3-acetic acid pool:Denovo synthesis,conjugate hydrolysis,and indole-3-butyric acidβ-oxidation[J].Journal of Plant Growth Regulation,2001,20(3):198-216.
24.Kochhar S,Singh S P,Kochhar V K.Effect of auxins and associated biochemical changes during clonal propagation of the biofuel plant-Jatrophacurcas[J].Biomass and Bioenergy,2008,32(12):1136-1143.
25.张玉臣,周再知,梁坤南,等.不同植物生长调节剂对白木香扦插生根的影响[J].林业科学研究,2010,23(2):278-282.Zhang Y C,Zhou Z Z,Liang K N,et al.Effectof different plant growth regulators on the rootingof Aquilaria sinensis cuttings[J].Forest Research,2010,23(2):278-282.
26.徐笑玥,佟兆庆,闫文涛,等.中华金叶榆嫩枝扦插繁殖技术[J].东北林业大学学报,2016,44(5):1-4.Xu X Y,Tong Z Q,Yan W T,et al.Soft cutting propagation technology of Ulmus pumila‘Zhonghuajinye’[J].Journal of Northeast Forestry University,2016,44(5):1-4.
27.吕跃东,姚颖,吕跃伟,等.不同激素和基质对黑穗醋栗扦插繁殖的影响[J].经济林研究,2015,33(4):144-147.LüY D,Yao Y,LüY W,et al.Effects of different hormones and media on cutting propagation in black currant[J].Nonwood Forest Research,2015,33(4):144-147.
28.闫海霞,卢家仕,黄昌艳,等.萘乙酸和吲哚丁酸对月季扦插成活效果的影响[J].南方农业学报,2013,44(11):1870-1873.Yan H X,Lu J S,Huang C Y,et al.Effects of NAA and IBA on survival of rosehardwood cutting[J].Journal of Southern Agriculture,2013,44(11):1870-1873.
29.魏树强.杂交鹅掌楸扦插繁殖技术与生根机理研究[D].南京:南京林业大学,2009.Wei S Q.Studies on the cutting propagation technology and rooting mechanism of Liriodendron chinese×L.tulipifera[D].Nanjing:Nanjing Forestry University,2009.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |