吡唑并嘧啶衍生物BDO-1诱导黄瓜对枯萎病的抗性

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英文篇名：A Pyrimidine Derivative Confers Cucumber Induced Resistance to Fusarium Wilt 作者：石延霞 ; 张晓慧 ; 徐玉芳 ; 谢学文 ; 柴阿丽 ; 李宝聚 英文作者：SHI Yanxia;ZHANG Xiaohui;XU Yufang;XIE Xuewen;CHAI Ali;LI Baoju;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences;School of Pharmacy,East China University of Science and Technology; 关键词：黄瓜 ; 吡唑并嘧啶衍生物 ; 黄瓜枯萎病 ; 诱导抗病性 英文关键词：cucumber;;pyrazolopyrimidine derivative;;cucumber wilt disease;;induced disease resistance 中文刊名：YYXB 英文刊名：Acta Horticulturae Sinica 机构：中国农业科学院蔬菜花卉研究所;华东理工大学药学院; 出版日期：2019-01-10 14:43 出版单位：园艺学报 年：2019 期：v.46 基金：国家重点研发计划项目(2016YFD0201000);; 中国农业科学院创新工程项目(CAAS-ASTIP-IVFCAAS);; 农业部园艺作物生物学与种质创制重点实验室开放课题(IVF2017) 语种：中文; 页：YYXB201905006 页数：14 CN：05 ISSN：11-1924/S 分类号：66-79

摘要

研究了新型植物化学抗病激活剂——吡唑并嘧啶衍生物(E)–N–(2–氟–4–三氟甲基苯乙烯基)–1–甲基–1H–吡唑并[3,4-d]嘧啶–4–胺(简称BDO-1)诱导黄瓜抗枯萎病(Fusarium oxysporum f.sp.cucumerinum)的作用。菌丝生长速率法测定表明BDO-1对F.cucumerinum无抑菌活性。黄瓜叶面喷雾10 mg·L~(-1) BDO-1(每5 d喷1次,共5次),然后接种F. oxysporum,结果表明BDO-1对黄瓜具有较好的诱导抗病作用,诱导抗病效果达53.28%。利用激光扫描共聚焦显微镜观察发现,BDO-1诱导后减缓了F.oxysporum对黄瓜的侵染速度。荧光显微观察发现,黄瓜体内次生代谢物质胼胝质在BDO-1处理后3 d大量沉积,抗氧化物质H2O2含量在诱导后1 d出现明显升高。采用Real-Time PCR检测发现,BDO-1诱导后黄瓜体内防御相关基因CAT、PAL、LOX和EIN2表达量从接种后1d到7d均有显著升高,PR1和SOD表达量在接种后1 d时较高,之后显著降低。本研究为BDO-1作为新型抗病激活剂的开发及田间应用提供了参考依据。
        In this study,a new type of disease-resistant activator named BDO-1,(E)-N-[2-fluoro-4-(trifluoromethyl)styryl]-1-met-hyl-1 H-pyrazolo[3,4-d]pyrimidin-4-amine,which belongs to pyrazolium pyrimidine derivative was reported and studied. To reveal the resistance mechanism of BDO-1,the induced resistance effect of the activator to cucumber against Fusarium oxysporum f. sp. cucumerinum was investigated. The antifungal activity of BDO-1 was detected by using plate growth rate method,and the result showed that BDO-1 had no antagonistic effect against F. oxysporum. The leaves were treated with 10 mg · L~(-1) BOD-1 by foliar spraying method at two-leaf and one-hearted cucumber stage. A total of five times were induced in 25 days and the pathogen F. oxysporum was inoculated after 24 h of last induction.The induced assay results indicated that the induced resistance effect of BDO-1 to cucumber against F. oxysporum was 53.28%. In addition,laser confocal microscopy confirmed that the infection rates of F. oxysporum on cucumber were inhibited by induction of BDO-1. Meanwhile,fluorescence microscopy showed that the secondary metabolite callose accumulated in cucumber plants on the 3rd day post-inoculation,and the highest content of anti-oxidant H_2O_2 appeared in cucumber plants on the 1st day post-inoculation. Besides,qRT-PCR revealed that BDO-1 could induce expression of six disease-resistant defense genes. After BDO-1 induction,the expression levels of CAT,PAL,LOX and EIN2 in the defense genes of root defense tissues increased significantly from 1st day to 7th day post-inoculation,and the expression levels of PR1 and SOD were higher at 1st day post-inoculation,significantly reduced in 3rd day and 7th day post-inoculation. This study provides an insight and basis for the development and application of BDO-1 as a new type of disease-resistant activator.

引文

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