GPR120对奶牛脂肪细胞脂代谢的调节作用
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摘要
为研究GPR120对奶牛脂肪细胞脂合成和脂分解的影响,缓解围产期奶牛出现的脂代谢紊乱问题,在体外培养的奶牛脂肪细胞中分别沉默GPR120和激活GPR120,采用Western Blot、qRT-PCR等技术检测脂代谢基因变化。结果表明:沉默GPR120组奶牛脂肪细胞脂合成关键酶、脂滴包被蛋白(PLIN1)和甘油三酯(TG)含量显著降低;添加GPR120激动剂组奶牛脂肪细胞脂合成关键酶和TG含量升高,激素敏感酯酶(HSL)基因表达量降低。这说明,GPR120在体外培养的奶牛脂肪细胞中能促进脂肪细胞的脂合成,抑制脂肪细胞的脂分解。
In order to study the effects of GPR120 on lipid synthesis and lipolysis of bovine adipocytes and alleviate the problem of lipid metabolism disorder in perinatal dairy cows,GPR120 was silenced and GPR120 was activated in bovine adipocytes cultured in vitro,and Western Blot and experimental techniques such as qRT-PCR were used to detect changes in lipid metabolism genes. The results showed that compared with the control group,the levels of lipid synthesis,lipid droplet coating protein(PLIN1) and triglyceride(TG) in adipocytes of the GPR120 group were significantly lower than those in the control group. Compared with the control group; in the GPR120 agonist group,the key enzymes and TG content of lipid synthesis in dairy cows were increased,and the expression of hormone-sensitive esterase(HSL) gene was decreased. The above experimental results show that GPR120 can promote lipid synthesis and inhibit lipolysis of bovine adipocytes cultured in vitro.
In order to study the effects of GPR120 on lipid synthesis and lipolysis of bovine adipocytes and alleviate the problem of lipid metabolism disorder in perinatal dairy cows,GPR120 was silenced and GPR120 was activated in bovine adipocytes cultured in vitro,and Western Blot and experimental techniques such as qRT-PCR were used to detect changes in lipid metabolism genes. The results showed that compared with the control group,the levels of lipid synthesis,lipid droplet coating protein(PLIN1) and triglyceride(TG) in adipocytes of the GPR120 group were significantly lower than those in the control group. Compared with the control group; in the GPR120 agonist group,the key enzymes and TG content of lipid synthesis in dairy cows were increased,and the expression of hormone-sensitive esterase(HSL) gene was decreased. The above experimental results show that GPR120 can promote lipid synthesis and inhibit lipolysis of bovine adipocytes cultured in vitro.
引文
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[2] Ballard F J,Hanson R W. Metabolic changes in liver associated with spontaneous ketosis and starvation in cows[J]. Journal of Nutrition,1968,95(2):160-172.
[3] Murondoti A,Jorritsma R,Beynen A C,et al. Unrestricted feed intake during the dry period impairs the postpartum oxidation and synthesis of fatty acids in the liver of dairy cows[J].Journal of Dairy Science,2004,87(8):672-679.
[4] Walther T C,JR R V F. Lipid droplets and cellular lipid metabolism[J]. Annual Review of Biochemistry,2012,81(1):687-688.
[5] Wu Z,Bucher N L,Farmer S R. Induction of peroxisome proliferator-activated receptor gamma during the conversion of 3T3fibroblasts into adipocytes is mediated by C/EBP beta,C/EBP delta and glucocorticoids[J]. Mol Cell Biol,1996,16(1):4128-4136.
[6] Barroso I M,Gurnell V E,O Rahilly S. Dominant negative mutations in human PPAR gamma associated with severe insulin resistance,diabetes mellitus and hypertension[J]. Nature,1999,402(2):880-883.
[7] Yeh W C,Cao Z,M C K night S L. Cascade regulation of terminal adipocyte differentiation by three members of the C/EBP family of leucine zipper proteins[J]. Genes&Dev,1995,15(2):168-181.
[8] Tanaka T,Yoshida N,Akira S. Defective adipocyte differentiation in mice lacking the C/EBPβand/or C/EBPδgene EMBO[J]. Journal of Nutrition,1997,16(2):7432-7443.
[9] Amy R,Johnson J J M,Liza Makowski. The inflammation highway:metabolism accelerates inflammation traffic in obesity[J]. Immunological Reviews,2012,249(1):218-219.
[10] Wellhauser L,Belsham D D. Activation of the omega-3 fatty acid receptor GPR120 mediates anti-inflammatory actions in immortalized hypothalamicneurons[J]. Jneuro in Flammation,2014,11(1):60-61.
[11] OH DY,Talukdar S,Bae E J,et al.GPR120 is an omega-3fatty acid receptor mediating potent anti-inflammatory and insulin-sensitizing effects[J]. Cell,2010,142(2):687-698.
[12]李弯弯,魏云林,季秀玲. GPR120受体及其新药开发研究进展[J].药物生物技术,2017,24(5):459-463.
[13]张敏.能量负平衡奶牛肝脂沉积过程中SREBP-1c/Cidea通路的作用机制[D].长春:吉林大学,2016.
[14]殷立恒. PRL、GC对奶牛脂肪细胞和肝细胞脂代谢的影响[D].长春:吉林大学,2015.
[15]周秋格,刘畅,赖金伦,等.SIGIRR对奶牛乳腺上皮细胞TLR4致炎信号通路的负调控作用[J].中国兽医学报,2018,38(2):404-410.
[16]秦王禹.花荵水煎液对实验性高脂血症动物作用的研究[J].吉林农业大学学报,2005,27(3):296-298.
[17] Yilmaz S,Pekdemir M,Sarisoy H T,et al.Post-traumatic cerebral infarction:a rare complication in a pediatric patient after mild head injury[J].Ulus Travma Acil Cerrahi Derg,2011,17(2):186-188.
[18] Jang Heung-Jin,Zaza Kokrasbvili,Michael J Theodorakis,et al. Gut-expressed GUSTDUCIN and taste receptors regulate secretion of glucagon-like peptide-1[J]. PNAS,2007,104(38):15069-15074.
[19] Dahlman I,Arner P. Obesity and polymorphisms in genes regulating human adipose tissue[J]. Int J Obes(Lond),2007,31(11):1629-1641.
[20] Cornish Jillian,Gibbon Alastair Mac,Lin Jianming,et al.Modulation of OSTEOCLAS to genesis by fatty acids[J]. Endocrinology,2008,64(2):111-112.
[21] Du Xi Liang,Shen Taiyu,Wang Heyuan,et al. Adaptations of hepatic lipid metabolism and mitochondria in dairy cows with mild fatty liver[J]. Journal of Dairy Science,2018(145546)101:1-15.
[22]云巾宴,于永生,曹阳,等.葛根素对延黄牛原代脂肪细胞分化的影响[J].吉林农业大学学报,2018,40(2):223-228.
[23] Doyle M E,Egan J M,Mechanisms of action glucagonlike peptide 1 in the pancreas[J]. Pharmacol,2007,113(3):546-593.
[24] Rayasam G V,Tulasi V K,Davis J A,et al. Fatty acid receptors as new therapeutic targets for diabetes[J]. Exp Opin Ther Targets,2007,11(5):661-671.
[25] Katsuma S,Hatae N,Yano T,et al. Free fatty acids inhibit serumdeprivation-induced apoptosis through GPR120 in a murineenteroendocrine cell line STC-1[J]. J Biol Chem,2005,280(20):1507-1515.
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