白介素-6在HIV合并马尔尼菲蓝状菌感染人群中的表达及作用初步研究
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摘要
目的探讨白介素-6(interleukin 6,IL-6)在人类免疫缺陷病毒(human immunodeficiency virus,HIV)合并马尔尼菲蓝状菌(talaromyces marneffei,TM)感染人群中的表达情况及作用。方法选择在南宁市第四人民医院未接受抗病毒治疗(antiretroviral therapy,ART)的HIV感染者27例,按是否合并TM感染情况分为HIV+TM组(12例)与HIV组(15例)。比较两组的人口学资料和外周血单核细胞(peripheral blood mononuclear cell,PBMC)中IL-6的表达水平。结果两组年龄、性别和病毒载量比较差异无统计学意义(P> 0.05),HIV+TM组的CD4~+T细胞计数低于HIV组,差异有统计学意义(P=0.018)。HIV+TM组中巨噬细胞(PBMC中的一个亚群)的IL-6表达水平高于HIV组,差异有统计学意义(P=0.039)。结论 HIV+TM组PBMC中的IL-6表达较HIV组高,这可能是导致巨噬细胞免疫过度激活,促进TM感染,影响病程进展的关键因素。
Objective To explore the expression and effect of interleukin 6(IL-6) in human immunodeficiency virus(HIV) and Talaromyces marneffei(TM) coinfection patients.Methods Twenty-seven HIV-infected patients who had not received antiretroviral therapy(ART) were selected from the Fourth People's Hospital of Nanning City,and were divided into HIV + TM group(12 cases) and HIV group(15 cases) according to the infection status of TM.The demographic data and the level of IL-6 in perpheral blood mononuclear cell(PBMC) were compared between the two groups.Results There were no significant differences in age,gender and viral load between the two groups(P > 0.05),while the CD4~+T cell count of the HIV + TM group was significantly lower than that of the HIV group(P = 0.018).The IL-6 expression level of macrophages(a subgroup of PBMC) in the HIV + TM group was significantly higher than that of the HIV group(P = 0.039).Conclusion The IL-6 expression of HIV + TM group in PBMC is higher than HIV group,which may lead to immune hyperactivation in macrophages and may be a critical factor to promote TM infection and disease progression.
Objective To explore the expression and effect of interleukin 6(IL-6) in human immunodeficiency virus(HIV) and Talaromyces marneffei(TM) coinfection patients.Methods Twenty-seven HIV-infected patients who had not received antiretroviral therapy(ART) were selected from the Fourth People's Hospital of Nanning City,and were divided into HIV + TM group(12 cases) and HIV group(15 cases) according to the infection status of TM.The demographic data and the level of IL-6 in perpheral blood mononuclear cell(PBMC) were compared between the two groups.Results There were no significant differences in age,gender and viral load between the two groups(P > 0.05),while the CD4~+T cell count of the HIV + TM group was significantly lower than that of the HIV group(P = 0.018).The IL-6 expression level of macrophages(a subgroup of PBMC) in the HIV + TM group was significantly higher than that of the HIV group(P = 0.039).Conclusion The IL-6 expression of HIV + TM group in PBMC is higher than HIV group,which may lead to immune hyperactivation in macrophages and may be a critical factor to promote TM infection and disease progression.
引文
1中国疾病预防控制中心,性病艾滋病预防控制中心,性病控制中心.2017年12月全国艾滋病性病疫情[J].中国艾滋病性病,2018,24(2):111.
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3 Samson RA,Yilmaz N,Houbraken J,et al.Phylogeny and nomenclature of the genus Talaromyces and taxa accommodated in Penicillium subgenus Biverticillium[J].Stud Mycol,2011,70(1):159-183.
4 Supparatpinyo K,Khamwan C,Baosoung V,et al.Disseminated Penicillium marneffei infection in southeast Asia[J].Lancet,1994,344(8915):110-113.
5 Jiang J,Meng S,Huang S,et al.Effects of Talaromycesmarneffei infection on mortality of HIV/AIDS patients in southern China:a retrospective cohort study[J].Clin Microbiol Infect,2019,25(2):233-241.
6 Akira S,Kishimoto T.IL-6 and NF-IL6 in acute-phase response and viral infection[J].Immunol Rev,1992,127:25-50.
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8 Masur H,Brooks JT,Benson CA,et al.Prevention and treatment of opportunistic infections in HIV-infected adults and adolescents:Updated Guidelines from the Centers for Disease Control and Prevention,National Institutes of Health,and HIV Medicine Association of the Infectious Diseases Society of America[J].Clin Infect Dis,2014,58(9):1308-1311.
9 Negorev D,Beier UH,Zhang T,et al.Human neutrophils can mimic myeloid-derived suppressor cells(PMN-MDSC)and suppress microbead or lectin-induced T cell proliferation through artefactual mechanisms[J].Sci Rep,2018,8(1):3135.
10帅波,沈霖,杨艳萍,等.加味青娥丸对模拟失重状态下小鼠骨显微结构和β-catenin及DKK-1表达水平的影响[J].中国临床新医学,2018,11(12):1186-1191.
11 Vanittanakom N,Cooper CR Jr,Fisher MC,et al.Penicillium marneffei infection and recent advances in the epidemiology and molecular biology aspects[J].Clin Microbiol Rev,2006,19(1):95-110.
12 Zheng J,Gui X,Cao Q,et al.A Clinical Study of Acquired Immunodeficiency Syndrome Associated Penicillium Marneffei Infection from a Non-Endemic Area in China[J].Plo S One,2015,10(6):e0130376.
13 Le T,Van Kinh N,Cuc NTK,et al.A Trial of Itraconazole or Amphotericin B for HIV-Associated Talaromycosis[J].N Engl J Med,2017,376(24):2329-2340.
14 Vanittanakom N,Cooper CR Jr,Fisher MC,et al.Penicillium marneffei infection and recent advances in the epidemiology and molecular biology aspects[J].Clin Microbiol Rev,2016,19(1):95-110.
15 Supparatpinyo K,Chiewchanvit S,Hirunsri P,et al.Penicillium marneffei infection in patients infected with human immunodeficiency virus[J].Clin Infect Dis,1992,14(4):871-874.
16 Chen J,Zhang RF,Shen YZ,et al.Clinical Characteristics and Prognosis of Penicilliosis among Human Immunodeficiency Virus-Infected Patients in Eastern China[J].Am J Trop Med Hyg,2017,96(6):1350-1354.
17 Jones SA.Recent insights into targeting the IL-6 cytokine family in inflammatory diseases and cancer[J].Nat Rev Immunol,2018,18(12):773-789.
18 Epeldegui M,Lee JY,Martinez AC,et al.Predictive Value of Cytokines and Immune Activation Biomarkers in AIDS-Related NonHodgkin Lymphoma Treated with Rituximab plus Infusional EPOCH(AMC-034 trial)[J].Clin Cancer Res,2016,22(2):328-336.
2李屏,莫武宁,李山,等.马尔尼菲青霉病的发病诱因及临床表现分析[J].中国临床新医学,2013,6(11):1050-1052.
3 Samson RA,Yilmaz N,Houbraken J,et al.Phylogeny and nomenclature of the genus Talaromyces and taxa accommodated in Penicillium subgenus Biverticillium[J].Stud Mycol,2011,70(1):159-183.
4 Supparatpinyo K,Khamwan C,Baosoung V,et al.Disseminated Penicillium marneffei infection in southeast Asia[J].Lancet,1994,344(8915):110-113.
5 Jiang J,Meng S,Huang S,et al.Effects of Talaromycesmarneffei infection on mortality of HIV/AIDS patients in southern China:a retrospective cohort study[J].Clin Microbiol Infect,2019,25(2):233-241.
6 Akira S,Kishimoto T.IL-6 and NF-IL6 in acute-phase response and viral infection[J].Immunol Rev,1992,127:25-50.
7 Hsu DC,Ma YF,Hur S,et al.Plasma IL-6 levels are independently associated with atherosclerosis and mortality in HIV-infected individuals on suppressive antiretroviral therapy[J].Aids,2016,30(13):2065-2074.
8 Masur H,Brooks JT,Benson CA,et al.Prevention and treatment of opportunistic infections in HIV-infected adults and adolescents:Updated Guidelines from the Centers for Disease Control and Prevention,National Institutes of Health,and HIV Medicine Association of the Infectious Diseases Society of America[J].Clin Infect Dis,2014,58(9):1308-1311.
9 Negorev D,Beier UH,Zhang T,et al.Human neutrophils can mimic myeloid-derived suppressor cells(PMN-MDSC)and suppress microbead or lectin-induced T cell proliferation through artefactual mechanisms[J].Sci Rep,2018,8(1):3135.
10帅波,沈霖,杨艳萍,等.加味青娥丸对模拟失重状态下小鼠骨显微结构和β-catenin及DKK-1表达水平的影响[J].中国临床新医学,2018,11(12):1186-1191.
11 Vanittanakom N,Cooper CR Jr,Fisher MC,et al.Penicillium marneffei infection and recent advances in the epidemiology and molecular biology aspects[J].Clin Microbiol Rev,2006,19(1):95-110.
12 Zheng J,Gui X,Cao Q,et al.A Clinical Study of Acquired Immunodeficiency Syndrome Associated Penicillium Marneffei Infection from a Non-Endemic Area in China[J].Plo S One,2015,10(6):e0130376.
13 Le T,Van Kinh N,Cuc NTK,et al.A Trial of Itraconazole or Amphotericin B for HIV-Associated Talaromycosis[J].N Engl J Med,2017,376(24):2329-2340.
14 Vanittanakom N,Cooper CR Jr,Fisher MC,et al.Penicillium marneffei infection and recent advances in the epidemiology and molecular biology aspects[J].Clin Microbiol Rev,2016,19(1):95-110.
15 Supparatpinyo K,Chiewchanvit S,Hirunsri P,et al.Penicillium marneffei infection in patients infected with human immunodeficiency virus[J].Clin Infect Dis,1992,14(4):871-874.
16 Chen J,Zhang RF,Shen YZ,et al.Clinical Characteristics and Prognosis of Penicilliosis among Human Immunodeficiency Virus-Infected Patients in Eastern China[J].Am J Trop Med Hyg,2017,96(6):1350-1354.
17 Jones SA.Recent insights into targeting the IL-6 cytokine family in inflammatory diseases and cancer[J].Nat Rev Immunol,2018,18(12):773-789.
18 Epeldegui M,Lee JY,Martinez AC,et al.Predictive Value of Cytokines and Immune Activation Biomarkers in AIDS-Related NonHodgkin Lymphoma Treated with Rituximab plus Infusional EPOCH(AMC-034 trial)[J].Clin Cancer Res,2016,22(2):328-336.
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