大鼠骨髓间充质干细胞对子宫内膜的损伤修复

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英文篇名：The experimental study of therapeutic effect of bone marrow mesenchymal stem cells transplantation on Rat endometrial damage 作者：林彤 ; 李冬宏 ; 张鲁榕 ; 郭玉佳 ; 陈丽红 ; 陈杏婷 ; 连梅 ; 林晨 英文作者：LIN Tong;LI Donghong;ZHANG Lurong;GUO Yujia;CHEN Lihong;CHEN Xingting;LIAN Mei;LIN Chen;Department of Reproductive Medicine, Maternal and Child Health Hospital,Longhua District, Shenzhen,Guangdong; 关键词：大鼠骨髓充质干细胞 ; 子宫损伤模型 ; 大鼠 ; SRY ; DNA 英文关键词：BMSCs;;damage endometrial;;Rat;;SRY DNA 中文刊名：FKLC 英文刊名：Chinese Journal of Clinical Obstetrics and Gynecology 机构：深圳市龙华区妇幼保健院生殖医学科;福建医科大学附属第一医院生殖医学中心;福建医科大学附属第一医院中心实验室;福建医科大学附属第一医院妇产科; 出版日期：2019-05-15 出版单位：中国妇产科临床杂志 年：2019 期：v.20 基金：福建省自然科学基金资助项目(2016J05184) 语种：中文; 页：FKLC201903017 页数：3 CN：03 ISSN：11-4967/R 分类号：54-56

摘要

目的探讨骨髓间充质干细胞(bone marrow mesenchymalstem cells,BMSCs)移植对大鼠子宫内膜损伤的修复作用。方法 45只雌性SD大鼠分为三组:正常组、模型组、BMSCs组,每组15只。除正常组外,其余两组大鼠采用95%乙醇宫腔注射法建立子宫内膜损伤模型。正常组不予任何干预,模型组造模后注射PBS溶液,BMSCs组注射体外分离培养的雄性SD大鼠BMSCs悬液。14 d后切除子宫,观察子宫内膜形态学改变、子宫内膜厚度、用免疫组织化学方法检测子宫内膜角蛋白、波形蛋白、整合素β3的表达,通过对移植后子宫内膜组织的SRY DNA原位杂交来定位源自雄性供体的细胞。结果与正常组相比,模型组大鼠子宫内膜厚度显著变薄[(276.25±59.46)μm vs(413.52±68.67)μm,P <0.01],而BMSCs组大鼠子宫内膜的厚度[(347.22±87.56)μm]较模型组显著增加(P <0.05)。与正常组相比,模型组大鼠子宫内膜角蛋白、波形蛋白、整合素β3表达明显减少(P <0.01),而BMSCs组较模型组明显增加(P <0.05)。BMSCs组损伤侧子宫内膜组织中有SRY阳性细胞的定植,模型组则未见。结论 BMSCs移植能不同程度地促进大鼠损伤子宫内膜的修复。
        Objective To study the rapeutic effect of bone marrow mesenchymal stem cells(BMSCs) transplantation on the Rat endometrial damage. Methods 45 adult female SD rats were divided into three groups: normal control group,model control group and BMSCs group. The rat uterine endometrial damage models were established by intrauterine injection of 95% ethanol. The model controls were injected with PBS solution,the BMSCs group injected with stem cell suspension. 14 days later, the uterus were collected from the rats for observation of morphology, thickness, and the expression of cytokeratin,vimentin and integrin β3. In site hybridization was used to observe the expression of SRY-positive cells in the uterine endometrial tissue. Results Compared with the model controls,the rats of the BMSCs group showed significant thickening of the endometrium(P <0.05). The expressions of cytokeratin, vimentin and integrin β3 showed the highest in the normal control,the lowest in the model control group,and increased in theBMSCs group(P <0.05).It was shown that the BMSCs group appeared colonization of SRY-positive cells in the endometrium, while the model control group did not. Conclusion Bone marrow mesenchymal stem cell transplantation can promote the repair of damaged uterine endometrium in rats.

引文

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