miRNA-137在膀胱癌中的表达情况及对膀胱癌细胞侵袭和迁移的影响

详细信息    查看全文 | 推荐本文 |

英文篇名：miRNA-137 expression in bladder cancer and the impact on the invasion and migration of bladder cancer cells 作者：林学勇 ; 李东 英文作者：LIN Xueyong;LI Dong;Department of General Surgery, Anting Hospital of Shanghai Jiading District; 关键词：膀胱癌 ; miRNA-137 ; 表达水平 ; 生物学特性 英文关键词：bladder cancer;;miRNA-137;;expression level;;biological characteristic 中文刊名：AZJZ 英文刊名：Oncology Progress 机构：上海市嘉定区安亭医院外科; 出版日期：2019-05-10 出版单位：癌症进展 年：2019 期：v.17 语种：中文; 页：AZJZ201909026 页数：3 CN：09 ISSN：11-4971/R 分类号：102-104

摘要

目的探讨miRNA-137在膀胱癌中的相对表达量及其对膀胱癌细胞侵袭和迁移能力的影响。方法选取30例膀胱癌患者的膀胱癌组织和癌旁组织标本。实时定量逆转录聚合酶链反应(RT-PCR)检测膀胱癌组织和癌旁组织中的miRNA-137的相对表达量,检测不同病理分级膀胱癌患者膀胱癌组织中miRNA-137的表达量及其与膀胱癌病理分级的相关性。将mimic-NC、miRNA-137 mimic、miRNA-137 inhibitor质粒转染至膀胱癌T24细胞,并分别作为空白组、过表达组和低表达组,RT-PCR法检测3组细胞miRNA-137的相对表达量,Transwell小室检测3组细胞的迁移和侵袭能力。结果浸润性膀胱癌组织中miRNA-137的相对表达量明显高于非浸润性膀胱癌组织和癌旁组织(P﹤0.01);病理分级为Ⅳ级的膀胱癌患者膀胱癌组织中miRNA-137的相对表达量明显高于病理分级为Ⅰ、Ⅱ和Ⅲ级的患者(P﹤0.01)。过表达组迁移和侵袭细胞数目均高于空白组细胞(P﹤0.05);低表达组迁移和侵袭细胞数目均低于空白组细胞(P﹤0.05)。结论浸润性膀胱癌组织和病理分级为Ⅳ级的膀胱癌组织中miRNA-137的相对表达量较高,上调miRNA-137的表达可以促进膀胱癌细胞侵袭和迁移,抑制其表达可以抑制膀胱癌细胞的迁移和侵袭。
        Objective To investigate the relative expression level of miRNA-137 in bladder cancer, and to investigate the effect on the invasion and migration of bladder cancer cells. Method Specimens of bladder cancer tissues and adjacent tissues from 30 patients with bladder cancer were selected. Real-time reverse transcription-polymerase chain reaction(RT-PCR) was applied to detect the relative expression of miRNA-137 in collected specimens. To detect the expression of miRNA-137 in bladder cancer tissues of bladder cancer patients with different pathological grades and its correlation with the pathological grades of bladder cancer. Into the bladder cancer T24 cells, mimic-NC, miRNA-137 mimic, and miRNA-137 inhibitor plasmid were transfected as blank control group, over expression group and low expression group, again,the RT-PCR was utilized to determine the relative expression of miRNA-137 in the 3 groups, moreover, Transwell assay was employed to detect the invasion and migration ability of these cells. Result The relative expression level of miRNA-137 in invasive bladder cancer tissues was higher than that of non-invasive bladder cancer tissues and adjacent tissues, the differences were statistically significant(P<0.01); the relative expression level of miRNA-137 in bladder cancer tissues of patients with bladder cancer of pathological grade IV was higher than that of patients with pathological grade I, II, and III diseases(P<0.01). The number of migrated cells and invasive cells in the overexpression group was significantly higher than that in the blank control group(P<0.05); while that in low expression group was evidently lower than that in blank control group(P<0.05). Conclusion The relative expression level of miRNA-137 is higher in invasive bladder cancer tissues and pathological grade IV bladder cancer tissues. The up-regulation of miRNA-137 can promote invasion and migration of bladder cancer cells, while inhibiting miRNA-137 expression will restrain bladder cancer cell migration and invasion.

引文

[1] Tang K, Wang C, Chen Z, et al. Clinicopathologic and prognostic significance of p21(cip1/waf1)expression in bladder cancer[J]. Int J Clin Exp Pathol, 2015, 8(5):4999-5007.
    [2] Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015[J]. CA Cancer J Clin, 2016, 66(2):115-132.
    [3] Guo J, Xia B, Meng F, et al. mi R-137 suppresses cell growth in ovarian cancer by targeting AEG-1[J]. Biochem Biophys Res Commun, 2013, 441(2):357-363.
    [4] Chen Q, Chen X, Zhang M, et al. mi R-137 is frequently down-regulated in gastric cancer and is a negative regulator of Cdc42[J]. Dig Dis Sci, 2011, 56(7):2009-2016.
    [5] Bier A, Giladi N, Kronfeld N, et al. Micro RNA-137 is downregulated in glioblastoma and inhibits the stemness of glioma stem cells by targeting RTVP-1[J]. Oncotarget,2013, 4(5):665-676.
    [6] Shimizu T, Suzuki H, Nojima M, et al. Methylation of a panel of micro RNA genes is a novel biomarker for detection of bladder cancer[J]. Eur Urol, 2013, 63(6):1091-1100.
    [7] Adam L, Wszolek MF, Liu CG, et al. Plasma micro RNA profiles for bladder cancer detection[J]. Urol Oncol, 2013,31(8):1701-1708.
    [8]刘默睦. RNA干扰Livin基因的表达对膀胱癌细胞凋亡及细胞周期的影响研究[D].长沙:中南大学, 2014.
    [9] Guancial EA, Bellmunt J, Yeh S, et al. The evolving understanding of micro RNA in bladder cancer[J]. Urol Oncol,2014, 32(1):e31-e40.
    [10] Yoshino H, Seki N, Itesako T, et al. Aberrant expression of micro RNAs in bladder cancer[J]. Nat Rev Urol, 2013, 10(7):396-404.
    [11] Brenner B, Hoshen MB, Purim O, et al. Micro RNAs as a potential prognostic factor in gastric cancer[J]. World J Gastroenterol, 2011, 17(35):3976-3985.
    [12] Li P, Ma L, Zhang Y, et al. Micro RNA-137 down-regulates KIT and inhibits small cell lung cancer cell proliferation[J]. Biomed Pharmacother, 2014, 68(1):7-12.
    [13] Liang L, Li X, Zhang X, et al. Micro RNA-137, an HMGA1 target, suppresses colorectal cancer cell invasion and metastasis in mice by directly targeting FMNL2[J]. Gastroenterology, 2013, 144(3):624-635.
    [14] Althoff K, Beckers A, Odersky A, et al. Mi R-137 functions as a tumor suppressor in neuroblastoma by downregulating KDM1A[J]. Int J Cancer, 2013, 133(5):1064-1073.