区域地下水不同深度微生物群落结构特征
|
摘要
为了揭示区域地下水不同深度微生物群落结构特征及其与地下水环境相互作用关系,选取北京琉璃河地区,采集不同深度地下水样品,用于水化学分析和微生物16S rRNA基因V4-V5区测序.水化学分析结果显示,地下水中8种主要离子浓度随深度增加均呈减小趋势,Cl~-、SO_4~(2-)、NO_3~-变化规律显著,工业较发达区NO_3~-浓度达155.30mg/L,SO_4~(2-)浓度达321.00mg/L,部分浅层地下水受NO_3~-和SO_4~(2-)污染.微生物分析结果显示,地下水中微生物群落多样性受深度影响显著,随深度增加微生物群落组成丰富.地下水中优势菌门为Proteobacteria(26.2%～95.2%),优势菌属为Pseudomonas(1.5%～32.2%),不同深度微生物菌属组成差异明显,浅层、中层和深层地下水特有菌属数目分别为74,60,54.NO_3~-、SO_4~(2-)、深度是影响地下水微生物群落的主要因子,且NO_3~-、SO_4~(2-)浓度受地下水深度影响程度大.地下水深度是影响微生物群落结构差异的重要原因.
To reveal the structure characteristics of microbial communities in regional groundwater at different depths and the interaction between microbial communities and groundwater environment,Liuli River district located in the southwestern Beijing was selected to collect different deep groundwater samples for the hydrochemical analysis and the high-throughput sequencing of the V4-V5region of microbial 16S rRNA.The results of hydrochemistry showed that the concentration of eight main ions decreased with the increase of depth in groundwater,especially Cl~-,SO_4~(2-)and NO_3~-varied obviously.In industrial area,the highest concentrations of NO_3~-and SO_4~(2-)were up to 155.30mg/L and 321.00mg/L,respectively,where shallow groundwater was contaminated with NO_3~-and SO_4~(2-).Microbial analysis showed that the diversity of microbial community was strongly affected by groundwater depth,and microbial abundance increased with the increase of groundwater depth.Proteobacteria(26.2%～95.2%)was the dominant phylum and Pseudomonas(1.5%～32.2%)was the dominant genus.The microbial community at different depths of groundwater was significantly different.The number of the specific genera of shallow,middle and deep aquifer were 74,60 and 54,respectively.NO_3~-,SO_4~(2-)and depth were the main factors affecting the microbial community.In addition,the concentrations of NO_3~-and SO_4~(2-)were closely related to depth.Groundwater depth was an important control factor to microbial community structure.
To reveal the structure characteristics of microbial communities in regional groundwater at different depths and the interaction between microbial communities and groundwater environment,Liuli River district located in the southwestern Beijing was selected to collect different deep groundwater samples for the hydrochemical analysis and the high-throughput sequencing of the V4-V5region of microbial 16S rRNA.The results of hydrochemistry showed that the concentration of eight main ions decreased with the increase of depth in groundwater,especially Cl~-,SO_4~(2-)and NO_3~-varied obviously.In industrial area,the highest concentrations of NO_3~-and SO_4~(2-)were up to 155.30mg/L and 321.00mg/L,respectively,where shallow groundwater was contaminated with NO_3~-and SO_4~(2-).Microbial analysis showed that the diversity of microbial community was strongly affected by groundwater depth,and microbial abundance increased with the increase of groundwater depth.Proteobacteria(26.2%～95.2%)was the dominant phylum and Pseudomonas(1.5%～32.2%)was the dominant genus.The microbial community at different depths of groundwater was significantly different.The number of the specific genera of shallow,middle and deep aquifer were 74,60 and 54,respectively.NO_3~-,SO_4~(2-)and depth were the main factors affecting the microbial community.In addition,the concentrations of NO_3~-and SO_4~(2-)were closely related to depth.Groundwater depth was an important control factor to microbial community structure.
引文
[1]Xu Y S.Evaluation of land subsidence by considering underground structures that penetrate the aquifers of Shanghai,China[J].Hydrogeology Journal,2012,20(8):1623-1634.
[2]Huang Y,Yang Y,Li J.Numerical simulation of artificial groundwater recharge for controlling land subsidence[J].Ksce Journal of Civil Engineering,2015,19(2):418-426.
[3]Shi X,Jiang S,Xu H,et al.The effects of artificial recharge of groundwater on controlling land subsidence and its influence on groundwater quality and aquifer energy storage in Shanghai,China[J].Environmental Earth Sciences,2016,75(3):1-18.
[4]黄健民,邓雄文,胡让全.广州金沙洲岩溶区地下水位变化与地面塌陷及地面沉降关系探讨[J].中国地质,2015,42(1):300-307.Huang J M,Deng X W,Hu R Q.The relationship between groundwater and ground collapse and land subsidence in Jinshazhou,Guangzhou city[J].Geology in China,2015,42(1):300-307.
[5]Barron O V,Barr A D,Donn M J.Evolution of nutrient export under urban development in areas affected by shallow watertable[J].Science of the Total Environment,2013,443(3):491-504.
[6]Luo S,Wu B,Xiong X,et al.Short-term toxicity of ammonia,nitrite and nitrate to early life stages of the rare minnow,Gobiocypris rarus[J].Environmental Toxicology&Chemistry,2016,35(6):1422-1427.
[7]Zuo R,Chen X,Li X,et al.Distribution,genesis,and pollution risk of ammonium nitrogen in groundwater in an arid loess plain,northwestern China[J].Environmental Earth Sciences,2017,76(17):629.
[8]杜新强,方敏,冶雪艳.地下水“三氮”污染来源及其识别方法研究进展[J].环境科学,2018,39(11):5266-5275.Du X Q,Fang M,Ye X Y.Research progress on the source of inorganic nitrogen pollution in groundwater and identification methods[J].Environmental Science,2018,39(11):5266-5275.
[9]李军,甄世军,张翠云,等.基因芯片在地下水污染研究中的应用[J].生物技术,2017,27(4):403-408.Li J,Zhen S J,Zhang C Y,et al.Application of microarray in contamination of groundwater[J].Biotechnology,2017,27(4):403-408.
[10]Zobell C E.Assimilation of hydrocarbons by microorganisms[J].Advances in Enzymology&Related Subjects of Biochemistry,1950,10(10):443-486.
[11]陈辉伦.石油污染浅层含水层的微生物修复研究进展[J].安全与环境工程,2015,22(1):66-72.Chen H L.Research progress in microbial remediation of petroleumcontaminated shallow aquifer[J].Safety and Environmental Engineering,2015,22(1):66-72.
[12]李元杰,王森杰,张敏,等.土壤和地下水污染的监控自然衰减修复技术研究进展[J].中国环境科学,2018,38(3):1185-1193.Li Y J,Wang S J,Zhang M,et al.Research progress of monitored natural attenuation remediation technology for soil and groundwater pollution[J].China Environmental Science,2018,38(3):1185-1193.
[13]刘虹,张兰英,刘娜,等.生物可渗透性反应墙修复石油烃污染地下水的效果及微生物多样性研究[J].环境污染与防治,2013,35(3):1-4.Liu H,Zhang L Y,Liu N,et al.Performance and microbial diversity of bio-PRB reactor in the remediation of groundwater contaminated by petroleum hydrocarbons[J].Environmental pollution and control,2013,35(3):1-4.
[14]Zhou A X,Zhang Y L,Dong T Z,et al.Response of the microbial community to seasonal groundwater level fluctuations in petroleum hydrocarbon-contaminated groundwater[J].Environ Sci Pollut Res Int,2015,22(13):10094-10106.
[15]吴蔓莉,祁燕云,祝长成,等.堆肥对土壤中石油烃的去除及微生物群落的影响[J].中国环境科学,2018,38(8):3042-3048.Wu M L,Qi Y Y,Zhu C C,et al.Influence of compost amendment on hydrocarbon degradation and microbial communities in petroleum contaminated soil[J].China Environmental Science,2018,38(8):3042-3048.
[16]Yusoff M Z M,Hu A,Feng C,et al.Influence of pretreated activated sludge for electricity generation in microbial fuel cell application[J].Bioresource technology,2013,145:90-96.
[17]蔡萍萍,宁卓,何泽,等.采油井场土壤微生物群落结构分布[J].环境科学,2018,39(7):1-14.Cai P P,Ning Z,He Z,et al.Microbial community distribution in soils of an oil exploitation site[J].Environmental Science,2018,39(7):1-14.
[18]徐飞,蔡体久,杨雪,等.三江平原沼泽湿地垦殖及自然恢复对土壤细菌群落多样性的影响[J].生态学报,2016,36(22):7412-7421.Xu F,Cai T J,Yang X,et al.Effect of cultivation and natural restoration on soil bacterial community diversity in marshland in the Sanjiang Plain[J].Acta Ecologica Sinica,2016,36(22):7412-7421.
[19]GB/T 14848-2017地下水质量标准[S].GB/T 14848-2017 Standard for groundwater quality[S].
[20]Fernando T W,David N L.Non-agricultural sources of groundwater nitrate:a review and case study[J].Water Research,2005,39(1):3-16.
[21]Denk T R A,Mohn J,Decock C,et al.The nitrogen cycle:A review of isotope effects and isotope modeling approaches[J].Soil Biology&Biochemistry,2017,105:121-137.
[22]World Health Organization.Guidelines for drinking-water quality.Vol.1,(3rd Ed)[M].World Health Organization,2008.
[23]左锐,王金生,杨洁,等.滨海石化项目地下水环境影响评价的关键问题[J].水文地质工程地质,2010,37(3):97-101.Zuo R,Wang J S,Yang J,et al.Key problems of the groundwater environmental assessment for petrochemical projects in littoral zones[J].Hydrogeology and Engineering geology,2010,37(3):97-101.
[24]Deng Y,Zhang P,Qin Y,et al.Network succession reveals the importance of competition in response to emulsified vegetable oil amendment for uranium bioremediation.[J].Environmental Microbiology,2016,18(1):205-218.
[25]Widory D,Petelet-Giraud E,Negrel P,et al.Tracking the sources of nitrate in groundwater using coupled nitrogen and boron isotopes:Asynthesis[J].Environmental Science and Technology,2005,39(2):539-548.
[26]Zhang Q Q,Sun J Z,Liu J T,et al.Driving mechanism and sources of groundwater nitrate contamination in the rapidly urbanized region of south China[J].Journal of Contaminant Hydrology,2015,182:221-230.
[27]康鹏亮,黄廷林,张海涵,等.西安市典型景观水体水质及反硝化细菌种群结构[J].环境科学,2017,38(12):5174-5183.Kang P L,Huang Y L,Zhang H H,et al.Water quality and diversity of denitrifier community structure of typical scenic water bodies in Xi`an[J].Environmental Science,2017,38(12):5174-5183.
[28]Zhou M,Ye H,Zhao X.Isolation and characterization of a novel heterotrophic nitrifying and aerobic denitrifying bacterium Pseudomonas stutzeri,KTB for bioremediation of wastewater[J].Biotechnology&Bioprocess Engineering,2014,19(2):231-238.
[29]Spring S,Wagner H P,Kampfer P.Malikia granosa gen.nov.sp.nov.a novel polyhydroxyalkanoate-and polyphosphate-accumulating bacterium isolated from activated sludge,and reclassification of Pseudomonas spinosa as Malikia spinosa comb.nov[J].International Journal of Systematic and Evolutionary Microbiology,2005,55(2):621-629.
[30]Ehrich S,Behrens D,Lebedeva E,et al.A new obligately chemolithoautotrophic,nitrite-oxidizing bacterium,Nitrospira moscoviensis sp.nov.and its phylogenetic relationship[J].Archives of Microbiology,1995,164(1):16-23.
[31]Koch H,Galushko A,Albertsen M,et al.Growth of nitrite-oxidizing bacteria by aerobic hydrogen oxidation[J].Science,2014,345(6200):1052-1054.
[32]Fujitani H,Ushiki N,Tsuneda S,et al.Isolation of sublineage INitrospira by a novel cultivation strategy[J].Environmental Microbiology,2013,16(10):3030-3040.
[33]Krell T,Lacal J,Reyes-Darias J A,et al.Bioavailability of pollutants and chemotaxis[J].Current Opinion in Biotechnology,2013,24(3):451-456.
[34]Parales R E,Ditty J L,Harwood C S.Toluene-degrading bacteria are chemotactic towards the environmental pollutants benzene,toluene,and trichloroethylene[J].Applied and Environmental Microbiology,2000,66(9):4098-4104.
[35]Tremaroli V,Suzzi C V,Fedi S,et al.Tolerance of Pseudomonas pseudoalcaligenes KF707to metals,polychlorobiphenyls and chlorobenzoates:effects on chemotaxis-,biofilm-and planktonicgrown cells[J].Fems Microbiology Ecology,2010,74(2):291-301.
[36]Janmejay P,Sharma N K,Fazlurrahman K,et al.Chemotaxis of Burkholderia sp.Strain SJ98towards chloronitroaromatic compounds that it can metabolise[J].Bmc Microbiology,2012,12:19.
[37]Smith M B,Rocha A M,Smillie C S,et al.Natural bacterial communities serve as quantitative geochemical biosensors[J].Mbio.,2015,6(3):e00326.
[38]Kavitha S,Selvakumar R,Sathishkumar M,et al.Nitrate removal using Brevundimonas diminuta MTCC 8486from ground water[J].Water Science&Technology A Journal of the International Association on Water Pollution Research,2009,60(2):517-524.
[39]刘新展,贺纪正,张丽梅.水稻土中硫酸盐还原微生物研究进展[J].生态学报,2009,29(8):4455-4463.Liu X Z,He J Z,Zhang L M.The sulfate-reducing bacteria and surfer cycle in paddy soil[J].Acta Ecologica Sinica,2009,29(8):4455-4463.
[2]Huang Y,Yang Y,Li J.Numerical simulation of artificial groundwater recharge for controlling land subsidence[J].Ksce Journal of Civil Engineering,2015,19(2):418-426.
[3]Shi X,Jiang S,Xu H,et al.The effects of artificial recharge of groundwater on controlling land subsidence and its influence on groundwater quality and aquifer energy storage in Shanghai,China[J].Environmental Earth Sciences,2016,75(3):1-18.
[4]黄健民,邓雄文,胡让全.广州金沙洲岩溶区地下水位变化与地面塌陷及地面沉降关系探讨[J].中国地质,2015,42(1):300-307.Huang J M,Deng X W,Hu R Q.The relationship between groundwater and ground collapse and land subsidence in Jinshazhou,Guangzhou city[J].Geology in China,2015,42(1):300-307.
[5]Barron O V,Barr A D,Donn M J.Evolution of nutrient export under urban development in areas affected by shallow watertable[J].Science of the Total Environment,2013,443(3):491-504.
[6]Luo S,Wu B,Xiong X,et al.Short-term toxicity of ammonia,nitrite and nitrate to early life stages of the rare minnow,Gobiocypris rarus[J].Environmental Toxicology&Chemistry,2016,35(6):1422-1427.
[7]Zuo R,Chen X,Li X,et al.Distribution,genesis,and pollution risk of ammonium nitrogen in groundwater in an arid loess plain,northwestern China[J].Environmental Earth Sciences,2017,76(17):629.
[8]杜新强,方敏,冶雪艳.地下水“三氮”污染来源及其识别方法研究进展[J].环境科学,2018,39(11):5266-5275.Du X Q,Fang M,Ye X Y.Research progress on the source of inorganic nitrogen pollution in groundwater and identification methods[J].Environmental Science,2018,39(11):5266-5275.
[9]李军,甄世军,张翠云,等.基因芯片在地下水污染研究中的应用[J].生物技术,2017,27(4):403-408.Li J,Zhen S J,Zhang C Y,et al.Application of microarray in contamination of groundwater[J].Biotechnology,2017,27(4):403-408.
[10]Zobell C E.Assimilation of hydrocarbons by microorganisms[J].Advances in Enzymology&Related Subjects of Biochemistry,1950,10(10):443-486.
[11]陈辉伦.石油污染浅层含水层的微生物修复研究进展[J].安全与环境工程,2015,22(1):66-72.Chen H L.Research progress in microbial remediation of petroleumcontaminated shallow aquifer[J].Safety and Environmental Engineering,2015,22(1):66-72.
[12]李元杰,王森杰,张敏,等.土壤和地下水污染的监控自然衰减修复技术研究进展[J].中国环境科学,2018,38(3):1185-1193.Li Y J,Wang S J,Zhang M,et al.Research progress of monitored natural attenuation remediation technology for soil and groundwater pollution[J].China Environmental Science,2018,38(3):1185-1193.
[13]刘虹,张兰英,刘娜,等.生物可渗透性反应墙修复石油烃污染地下水的效果及微生物多样性研究[J].环境污染与防治,2013,35(3):1-4.Liu H,Zhang L Y,Liu N,et al.Performance and microbial diversity of bio-PRB reactor in the remediation of groundwater contaminated by petroleum hydrocarbons[J].Environmental pollution and control,2013,35(3):1-4.
[14]Zhou A X,Zhang Y L,Dong T Z,et al.Response of the microbial community to seasonal groundwater level fluctuations in petroleum hydrocarbon-contaminated groundwater[J].Environ Sci Pollut Res Int,2015,22(13):10094-10106.
[15]吴蔓莉,祁燕云,祝长成,等.堆肥对土壤中石油烃的去除及微生物群落的影响[J].中国环境科学,2018,38(8):3042-3048.Wu M L,Qi Y Y,Zhu C C,et al.Influence of compost amendment on hydrocarbon degradation and microbial communities in petroleum contaminated soil[J].China Environmental Science,2018,38(8):3042-3048.
[16]Yusoff M Z M,Hu A,Feng C,et al.Influence of pretreated activated sludge for electricity generation in microbial fuel cell application[J].Bioresource technology,2013,145:90-96.
[17]蔡萍萍,宁卓,何泽,等.采油井场土壤微生物群落结构分布[J].环境科学,2018,39(7):1-14.Cai P P,Ning Z,He Z,et al.Microbial community distribution in soils of an oil exploitation site[J].Environmental Science,2018,39(7):1-14.
[18]徐飞,蔡体久,杨雪,等.三江平原沼泽湿地垦殖及自然恢复对土壤细菌群落多样性的影响[J].生态学报,2016,36(22):7412-7421.Xu F,Cai T J,Yang X,et al.Effect of cultivation and natural restoration on soil bacterial community diversity in marshland in the Sanjiang Plain[J].Acta Ecologica Sinica,2016,36(22):7412-7421.
[19]GB/T 14848-2017地下水质量标准[S].GB/T 14848-2017 Standard for groundwater quality[S].
[20]Fernando T W,David N L.Non-agricultural sources of groundwater nitrate:a review and case study[J].Water Research,2005,39(1):3-16.
[21]Denk T R A,Mohn J,Decock C,et al.The nitrogen cycle:A review of isotope effects and isotope modeling approaches[J].Soil Biology&Biochemistry,2017,105:121-137.
[22]World Health Organization.Guidelines for drinking-water quality.Vol.1,(3rd Ed)[M].World Health Organization,2008.
[23]左锐,王金生,杨洁,等.滨海石化项目地下水环境影响评价的关键问题[J].水文地质工程地质,2010,37(3):97-101.Zuo R,Wang J S,Yang J,et al.Key problems of the groundwater environmental assessment for petrochemical projects in littoral zones[J].Hydrogeology and Engineering geology,2010,37(3):97-101.
[24]Deng Y,Zhang P,Qin Y,et al.Network succession reveals the importance of competition in response to emulsified vegetable oil amendment for uranium bioremediation.[J].Environmental Microbiology,2016,18(1):205-218.
[25]Widory D,Petelet-Giraud E,Negrel P,et al.Tracking the sources of nitrate in groundwater using coupled nitrogen and boron isotopes:Asynthesis[J].Environmental Science and Technology,2005,39(2):539-548.
[26]Zhang Q Q,Sun J Z,Liu J T,et al.Driving mechanism and sources of groundwater nitrate contamination in the rapidly urbanized region of south China[J].Journal of Contaminant Hydrology,2015,182:221-230.
[27]康鹏亮,黄廷林,张海涵,等.西安市典型景观水体水质及反硝化细菌种群结构[J].环境科学,2017,38(12):5174-5183.Kang P L,Huang Y L,Zhang H H,et al.Water quality and diversity of denitrifier community structure of typical scenic water bodies in Xi`an[J].Environmental Science,2017,38(12):5174-5183.
[28]Zhou M,Ye H,Zhao X.Isolation and characterization of a novel heterotrophic nitrifying and aerobic denitrifying bacterium Pseudomonas stutzeri,KTB for bioremediation of wastewater[J].Biotechnology&Bioprocess Engineering,2014,19(2):231-238.
[29]Spring S,Wagner H P,Kampfer P.Malikia granosa gen.nov.sp.nov.a novel polyhydroxyalkanoate-and polyphosphate-accumulating bacterium isolated from activated sludge,and reclassification of Pseudomonas spinosa as Malikia spinosa comb.nov[J].International Journal of Systematic and Evolutionary Microbiology,2005,55(2):621-629.
[30]Ehrich S,Behrens D,Lebedeva E,et al.A new obligately chemolithoautotrophic,nitrite-oxidizing bacterium,Nitrospira moscoviensis sp.nov.and its phylogenetic relationship[J].Archives of Microbiology,1995,164(1):16-23.
[31]Koch H,Galushko A,Albertsen M,et al.Growth of nitrite-oxidizing bacteria by aerobic hydrogen oxidation[J].Science,2014,345(6200):1052-1054.
[32]Fujitani H,Ushiki N,Tsuneda S,et al.Isolation of sublineage INitrospira by a novel cultivation strategy[J].Environmental Microbiology,2013,16(10):3030-3040.
[33]Krell T,Lacal J,Reyes-Darias J A,et al.Bioavailability of pollutants and chemotaxis[J].Current Opinion in Biotechnology,2013,24(3):451-456.
[34]Parales R E,Ditty J L,Harwood C S.Toluene-degrading bacteria are chemotactic towards the environmental pollutants benzene,toluene,and trichloroethylene[J].Applied and Environmental Microbiology,2000,66(9):4098-4104.
[35]Tremaroli V,Suzzi C V,Fedi S,et al.Tolerance of Pseudomonas pseudoalcaligenes KF707to metals,polychlorobiphenyls and chlorobenzoates:effects on chemotaxis-,biofilm-and planktonicgrown cells[J].Fems Microbiology Ecology,2010,74(2):291-301.
[36]Janmejay P,Sharma N K,Fazlurrahman K,et al.Chemotaxis of Burkholderia sp.Strain SJ98towards chloronitroaromatic compounds that it can metabolise[J].Bmc Microbiology,2012,12:19.
[37]Smith M B,Rocha A M,Smillie C S,et al.Natural bacterial communities serve as quantitative geochemical biosensors[J].Mbio.,2015,6(3):e00326.
[38]Kavitha S,Selvakumar R,Sathishkumar M,et al.Nitrate removal using Brevundimonas diminuta MTCC 8486from ground water[J].Water Science&Technology A Journal of the International Association on Water Pollution Research,2009,60(2):517-524.
[39]刘新展,贺纪正,张丽梅.水稻土中硫酸盐还原微生物研究进展[J].生态学报,2009,29(8):4455-4463.Liu X Z,He J Z,Zhang L M.The sulfate-reducing bacteria and surfer cycle in paddy soil[J].Acta Ecologica Sinica,2009,29(8):4455-4463.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |