miR-200c抑制人乳腺癌MCF-7细胞糖代谢

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英文篇名：miR-200c could Inhibits Glucose Metabolism in Human Breast Cancer MCF-7 Cells 作者：陈华波 ; 张崇 ; 殷焦 ; 肖娟 ; 杨林 英文作者：Chen Huabo;Zhang Chong;Yin Jiao;Xiao Juan;Yang Lin;Hubei University of Arts and Science;Xiangyang First People's Hospital; 关键词：miR-200c ; 乳腺癌 ; MCF-7 ; 糖代谢 英文关键词：miR-200c;;Breast cancer;;MCF-7;;Glucose metabolism 中文刊名：GXNB 英文刊名：Genomics and Applied Biology 机构：湖北文理学院;襄阳市第一人民医院; 出版日期：2019-06-25 出版单位：基因组学与应用生物学 年：2019 期：v.38 基金：2018年地方高校国家级大学生创新创业训练计划项目(201810519002);; 湖北省教育厅科学技术研究项目(D20172603)共同资助 语种：中文; 页：GXNB201906065 页数：5 CN：06 ISSN：45-1369/Q 分类号：448-452

摘要

为探讨miR-200c对人乳腺癌MCF-7 (Michigan cancer foundation-7)细胞糖代谢水平的影响,本研究使用miR-200c mimics转染MCF-7细胞,通过定量即时聚合酶链锁反应(quantitative real time polymerase chain reaction, qRT-PCR)检测各组细胞miR-200c的表达水平;利用细胞计数盒(cell counting kit-8, CCK-8)检测miR-200c mimics转染对MCF-7细胞增殖的影响;通过葡萄糖试剂盒检测各组细胞葡萄糖的消耗,乳酸试剂盒检测各组细胞乳酸的释放量;通过蛋白质印迹法(Western blotting)检测各组细胞糖代谢相关酶己糖激酶(hexokinase 2, HK2)以及肌肉丙酮酸激酶同工酶2 (pyruvate kinase isozyme type M2, PKM2)蛋白表达水平。与miR-NC组相比,miR-200c mimics转染明显上调MCF-7细胞miR-200c m RNA水平;且miR-NC组和MCF-7组miR-200c mRNA水平没有明显差异;细胞计数盒(cell counting kit-8, CCK-8)检测结果显示,与miR-NC组和MCF-7组相比,miR-200c mimics转染不仅明显降低乳腺癌MCF-7细胞的细胞活力,而且显著减少乳腺癌MCF-7细胞葡萄糖的消耗;此外,Western blotting结果显示,miR-200c显著下调MCF-7细胞糖代谢相关酶HK2和PKM2的表达。综上结果表明,miR-200c会抑制人乳腺癌MCF-7细胞糖代谢。本研究成果为乳腺癌防治提供一定的参考价值。
        To investigate the impact of miR-200 c on glucose metabolism in breast cancer MCF-7(michigan cancer foundation-7) cell. MCF-7 cells were transfected with miR-200 c mimics, and, the m RNA level of miR-200 c in MCF-7 cell was measured by quantitative real time polymerase chain reaction(qRT-PCR). The effect of miR-200 c on MCF-7 cell proliferation was detected by cell counting kit(CCK-8). Glucose consumption was measured by glucose assay kits and the lactate production was measured by lactate assay kits. The expressions of glucose metabolism-related proteins, including hexokinase 2(HK2) as well as pyruvate kinase isozyme type M2(PKM2), were assayed by Western blotting. Compared to miR-NC group, miR-200 c mimics transfection dramatically increased the mRNA expression of miR-200 c in MCF-7 cell. And the difference of miR-200 c mRNA expression between miR-NC group and MCF-7 group had no significance. Data from CCK-8 showed that compared with the miR-NC group and the MCF-7 group, miR-200 c mimics transfection not only significantly reduced the cell viability of breast cancer MCF-7 cells, but also significantly reduced the glucose consumption of breast cancer MCF-7 cells. Furthermore, data from Western blotting exhibited that miR-200 c dramatically decreased the expression of the enzymes involved in glucose metabolism in cells, HK2 as well as PKM2 in MCF-7 cell. In summary, miR-200 c could inhibits glucose metabolism in human breast cancer MCF-7 cells. This research provide a certain reference value for breast cancer prevention and treatment.

引文

Bell K.E.,Di Sebastiano K.M.,Vance V.,Hanning R.,Mitchell A.,Quadrilatero J.,Russell C.,Dubin J.A.,Bahl M.,Califaretti N.,Campbell C.,and Mourtzakis M.,2014,A comprehensive metabolic evaluation reveals impaired glucose metabolism and dyslipidemia in breast cancer patients early in the disease trajectory,Clin.Nutr.,33(3):550-557
    Chen J.,Chen Z.,and Huang J.,2018,Bioinformatics identification of dysregulated microRNAs in triple negative breast cancer based on microRNA expression profiling,Oncol.Lett.,15(3):3017-3023
    Du P.,and Zhang Q.Y.,2011,mi R-200 family and gastrointestinal tumors,Shijie Huaren Xiaohua Zazhi(World Chinese Journal of Digestology),19(29):3053-3057(杜平,张庆瑜,2011,mi R-200家族在消化系肿瘤中的研究进展,世界华人消化杂志,19(29):3053-3057)
    Gu J.,2010,The relationship between abnormal glncose metabolism related factors and breast cancer,Changwai Yu Changnei Yingyang(Parenteral&Enteral Nutrition),17(4):250-252,254(顾军,2010,糖代谢异常相关因素与乳腺癌,肠外与肠内营养,17(4):250-252,254)
    Karasneh R.A.,Murray L.J.,Hughes C.M.,and Cardwell C.R.,2015,Digoxin use after diagnosis of colorectal cancer and survival:a population-based cohort study,Cancer Epidemiol Biomarkers Prev.,24(11):1804-1807
    Kang D.M.,and Li S.Y.,2014,Recognition of abnormal glucose metabolism in malignant neoplasms,Zhongliuxue Zazhi(Journal of Chinese Oncology),20(8):644-647(康冬梅,李苏宜,2014,恶性肿瘤糖代谢异常的再认识,肿瘤学杂志,20(8):644-647)
    Li B.,2017,Recent proceedings in research of tumor-associated disorders of glucose metabolism,Zhongshan Daxue Xuebao(Journal of Sun Yat-sen University(Medical Sciences)),38(2):222-228(李博,2017,肿瘤糖代谢紊乱的研究进展和探索,中山大学学报(医学科学版),38(2):222-228)
    Shao X.,Ding Y.F.,Wang X.H.,and Shou Z.Q.,2018,Expression of HIF-1αand VEGF in breast cancer patients with disorder of carbohydrate metabolism and its correlation with angiogenesis,Zhonghua Quanke Yixue(Chinese Journal of General Practice),16(2):200-203(邵霞,丁耘峰,王晓红,寿志强,2018,HIF-1α和VEGF在不同糖代谢状态乳腺癌患者中的表达及其与微血管生成的相关性,中华全科医学,16(2):200-203)
    Su J.,and Zhang Q.Y.,2011,mi R-200 family and its mechanism,Guoji Zhongliuxue Zazhi(Journal of International Oncology),38(7):486-489(苏娟,张庆瑜,2011,mi R-200及其作用机制,国际肿瘤学杂志,38(7):486-489)