一株产高温纤维素酶菌株的筛选、鉴定及其酶学性质研究

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英文篇名：Isolation and identification of a strain producing thermostable cellulase and studying on its enzymatic properties 作者：杨力权 ; 周连广 ; 陈贵元 ; 桑鹏 英文作者：YANG Liquan;ZHOU Lianguang;CHEN Guiyuan;SANG Peng;College of Agriculture and Biological Science,Dali University;Basic Medical College,Dali University;Key Laboratory of Insect Biomedical Research and Development of Yunnan Province; 关键词：高温纤维素酶 ; 筛选 ; 鉴定 ; 酶学性质 英文关键词：thermostable cellulase;;isolation;;identification;;cellulase-properties 中文刊名：SLGZ 英文刊名：China Feed 机构：大理大学农学与生物科学学院;大理大学基础医学院;云南省昆虫生物医药研发重点实验室; 出版日期：2019-06-05 出版单位：中国饲料 年：2019 期：No.631 基金：国家自然科学基金项目(31660015);国家自然科学基金项目(31860243);; 云南省自然科学基金项目(2017FB024);云南省自然科学基金高校联合面上项目(2017FH001-032) 语种：中文; 页：SLGZ201911009 页数：5 CN：11 ISSN：11-2975/S 分类号：43-47

摘要

从云南大理市弥渡县石夹泉热泉的55℃底泥中筛选到1株高温纤维素酶的高产菌株,对其进行显微形态及生理生化特征、16S r RNA基因序列分析,将其初步鉴定为不动杆菌属(Acinetobacter sp.)的一株菌,命名为Acinetobacter sp.Cel-55。对其生长条件及酶学性质进行研究,结果表明:该菌株耐高温性较好,菌株在55℃仍能生长,菌株最适生长温度为37℃。所产纤维素酶最适酶活温度为75℃,最适反应pH为7.0。该酶具有良好的热稳定性,在75℃下,保温120 min仍能保持80%的活性。Zn~(2+)、Mn~(2+)、Ca~(2+)、K+、Mg~(2+)、Fe~(2+)、Cu~(2+)均对酶活力起一定的抑制作用,其中Mg~(2+)的抑制效果最为明显。
        A strain of high-temperature cellulase-producing bacteria was isolated from the sediment of Shijiaquan hot spring in Midu County,Dali City,Yunnan Province.And its morphology,physiological and biochemical characteristics,16 S rRNA gene sequence analysis were carried out.The strain was identified as a strain of Acinetobacter sp.,and named as Acinetobacter sp.Cel-55.The growth conditions and enzymatic properties of the strain were studied.The results showed that the strain was resistant to high temperature and could still grow at 55 ℃.The optimum growth temperature was 37 ℃.The optimum enzyme activity temperature for cellulase production was 75 ℃,and the optimum reaction pH was 7.0.Zn~(2+),Ca~(2+),Mg~(2+),Mn~(2+),K+,Fe~(2+),Cu~(2+)all played a role in inhibiting the activity of the enzyme,of which Mg~(2+)was the most obvious.

引文

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