大叶茜草精油挥发性物质抑菌及抗氧化活性研究
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摘要
以大叶茜草为原料,采用水蒸气蒸馏法提取大叶茜草挥发性物质,研究大叶茜草挥发性物质的抑菌和抗氧化活性.以金黄色葡萄球菌、枯草芽孢杆菌、沙门氏菌、大肠杆菌和志贺氏菌为供试菌种,测定抑菌圈直径,研究大叶茜草精油的抑菌活性;以天然抗氧化剂为对照,用DPPH,ABTS~(+·),H_2O_2和还原力4种不同的抗氧化体系检测大叶茜草精油挥发性成分的抗氧化活性.结果表明:大叶茜草精油对枯草芽孢杆菌和大肠杆菌抑菌性较差,对金黄色葡萄球菌、沙门氏菌和志贺氏菌表现出较好的抑制作用,大叶茜草精油对不同微生物生长繁殖的抑制作用有明显的差异;大叶茜草精油具有较好的清除DPPH,ABTS~(+·)和H_2O_2的生物活性,还原能力相对维生素C较弱.试验结果表明,大叶茜草精油挥发性物质作为天然抗氧化剂、抑菌剂具有巨大的开发潜质.
In this research, Rubia schumanniana Pritzel was used as the raw material and steam distillation method was used to extract the essential oil of Valerianaofficinalis. The research is mainly about the antibacterial and antioxidative activities of E. oleifera essential oil. About the research method, staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Salmonella, Escherichia coli and Shigella were used as tested strains to find out the antibacterial activity of Eucalyptus grandiflora essential oil by measuring the diameter of the inhibitory zone. Natural antioxidantsused as controls, the antioxidant activity of Eucalyptus grandiflora essential oil was tested by four different antioxidant systems: DPPH, ABTS~(+·), hydrogen peroxide, and reducing power. The results show that the essential oil of E. grandis had the lowest bacteriostatic effect against Bacillus subtilis and E. coli, and that good inhibition against Bacillus cereus, Staphylococcus aureus, Salmonella and Shigella. The inhibitory effects on growth and reproduction for different microbe are significantly different. The Eucalyptus grandis essential oil do better in decreasing the biological activity of DPPH, ABTS~(+·) and hydrogen peroxide, and its reducing ability is weaker than vitamin C. The test results suggest that, as a natural antioxidantand antibacterial agent, Eucalyptus grandis essential oil has agreat potential to explore.
In this research, Rubia schumanniana Pritzel was used as the raw material and steam distillation method was used to extract the essential oil of Valerianaofficinalis. The research is mainly about the antibacterial and antioxidative activities of E. oleifera essential oil. About the research method, staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Salmonella, Escherichia coli and Shigella were used as tested strains to find out the antibacterial activity of Eucalyptus grandiflora essential oil by measuring the diameter of the inhibitory zone. Natural antioxidantsused as controls, the antioxidant activity of Eucalyptus grandiflora essential oil was tested by four different antioxidant systems: DPPH, ABTS~(+·), hydrogen peroxide, and reducing power. The results show that the essential oil of E. grandis had the lowest bacteriostatic effect against Bacillus subtilis and E. coli, and that good inhibition against Bacillus cereus, Staphylococcus aureus, Salmonella and Shigella. The inhibitory effects on growth and reproduction for different microbe are significantly different. The Eucalyptus grandis essential oil do better in decreasing the biological activity of DPPH, ABTS~(+·) and hydrogen peroxide, and its reducing ability is weaker than vitamin C. The test results suggest that, as a natural antioxidantand antibacterial agent, Eucalyptus grandis essential oil has agreat potential to explore.
引文
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[18] 卞京军,程密密,罗思源,等.黑壳楠叶片精油挥发性成分的GC/MS鉴定与应用分析 [J].西南大学学报(自然科学版),2014,36(10):82-88.
[19] BAKKALI F,AVERBECK S,AVERBECK D,et al.Biological Effects of Essential Oils-a Review [J].Food & Chemical Toxicology,2008,46(2):446-475.
[20] ABDOUL LATIF F,EDOU P,EBA F,et al.Antimicrobial and Antioxidant Activities of Essential Oil and Methanol Extract of Jasminumsambac from Djibouti [J].African Journal of Plant Science,2010,4(3):38-43.
[2] 楼之岑,秦波.常用中药材品种整理和质量研究:北方编:第三册 [M].北京:北京医科大学中国协和医科大学联合出版社,2003.
[3] 肖培根.新编中药志I [M].北京:化学工业出版社,2002.
[4] 国家药典委员会.中华人民共和国药典:一部 [M].北京:中国医药科技出版社,2010.
[5] 李海峰,肖凌云,张菊,等.茜草化学成分及其药理作用研究进展 [J].中药材,2016,39(6):1433-1436.
[6] 权美平,郑翠平,马婷婷,等.茜草精油抗氧化及抗癌活性研究 [J].中国粮油学报,2016,31(4):89-93.
[7] PRIYDARSHI R,MELKANI A B,MOHAN L,et al.Terpenoid Composition and Antibacterial Activity of the Essential Oil from Inula Cappa (Buch-Ham.ex.D.Don) D C [J].Journal of Essential Oil Research,2016,28(2):172-176.
[8] 符群,徐明慧,王振宇.红松种鳞多酚超声波辅助提取优化工艺及其抗氧化性研究 [J].北京林业大学学报,2015,37(11):128-135.
[9] OZSOY N,CAN A,YANARDAG R,et al.Antioxidant Activity of Smilax Excelsa L.Leaf Extracts [J].Food Chemistry,2008,110(3):571-583.
[10] XU J G,HU Q P,LIU Y.Antioxidant and DNA-Protective Activities of Chlorogenic Acid Isomers [J].Journal of Agricultural & Food Chemistry,2012,60(46):11625-11630.
[11] LIU J,WANG C N,WANG Z Z,et al.The Antioxidant and Free-radical Scavenging Activities of Extract and Fractions from Corn Silk (Zea mays L.) and Related Flavone Glycosides [J].Food Chemistry,2011,126(1):261-269.
[12] 李春美,杜靖,谢笔钧.柚皮提取物的抑菌作用 [J].食品与发酵工业,2004,30(1):38-41,56.
[13] 马爱云,王海英,张岩,等.茴香精油的抑菌和抗氧化活性成分进展 [J].广东化工,2017,44(12):151-156.
[14] 樊梓鸾,张艳东,张华,等.红松松针精油抗氧化和抑菌活性研究 [J].北京林业大学学报,2017,39(8):98-103.
[15] 叶生梅,孙俊峰,庞宽壮,等.广玉兰花精油的化学成分、 抗氧化及抑菌活性分析 [J].中国粮油学报,2017,32(8):71-76.
[16] 薛山,袁园.柠檬精油提取工艺优化及抗氧化性测定 [J].中国食品添加剂,2017(5):183-188.
[17] 刘晓丽,赵谋明,崔春,等.超临界CO2萃取余甘子精油成分及其抗氧化活性研究 [J].西南大学学报(自然科学版),2007,29(5):122-127.
[18] 卞京军,程密密,罗思源,等.黑壳楠叶片精油挥发性成分的GC/MS鉴定与应用分析 [J].西南大学学报(自然科学版),2014,36(10):82-88.
[19] BAKKALI F,AVERBECK S,AVERBECK D,et al.Biological Effects of Essential Oils-a Review [J].Food & Chemical Toxicology,2008,46(2):446-475.
[20] ABDOUL LATIF F,EDOU P,EBA F,et al.Antimicrobial and Antioxidant Activities of Essential Oil and Methanol Extract of Jasminumsambac from Djibouti [J].African Journal of Plant Science,2010,4(3):38-43.
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