柴黄口服液对靶动物鸡安全性试验研究
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摘要
为研究柴黄口服液对靶动物鸡的安全性,将80只雏鸡随机分为四组。分别在饮水中添加0、1、3和5倍推荐剂量(0、1、3、5ml/L)的柴黄口服液,连续给药42 d。每天观察并记录各组鸡的临床体征,每周末称重1次,试验结束时测定各组鸡的血液生理生化指标,同时进行剖检。结果发现,柴黄口服液各剂量组鸡的精神状态、生长发育、血液生化生理指标、剖检以及组织显微结构等均未见明显异常,未出现中毒症状及死亡。综上所述,各浓度柴黄口服液对靶动物鸡均未见明显毒副作用,在临床上推荐以1 ml/L在鸡饮水中混饮。
In order to evaluate the target animal safety on Chaihuang oral solution in chickens,80 chicks were randomly divided into four groupsp. Chickens were treated with 0,1,3 and 5 times recommended doses( 0,1,3,5 ml/L) of Chaihuang oral solution to drinking water for 42 d respectively. The clinical signs of each group of chickens were observed and recorded every day and weighed once every weekend. At the end of the experiment,the blood physiological and biochemical indexes of each group of chickens were measured and dissected at the same time. The results showed that the mental state, growth and development, blood biochemical and physiological indexes,dissection,and tissue microstructure of different dose groups of Chaihuang oral solution were found no significant abnormalities and no toxic symptoms or death were found. Chaihuang oral solution has no obvious toxicity and side effect on the target animal chickens. It is safe to mix 1 ml/L Chaihuang oral solution in chicken drinking water clinically.
In order to evaluate the target animal safety on Chaihuang oral solution in chickens,80 chicks were randomly divided into four groupsp. Chickens were treated with 0,1,3 and 5 times recommended doses( 0,1,3,5 ml/L) of Chaihuang oral solution to drinking water for 42 d respectively. The clinical signs of each group of chickens were observed and recorded every day and weighed once every weekend. At the end of the experiment,the blood physiological and biochemical indexes of each group of chickens were measured and dissected at the same time. The results showed that the mental state, growth and development, blood biochemical and physiological indexes,dissection,and tissue microstructure of different dose groups of Chaihuang oral solution were found no significant abnormalities and no toxic symptoms or death were found. Chaihuang oral solution has no obvious toxicity and side effect on the target animal chickens. It is safe to mix 1 ml/L Chaihuang oral solution in chicken drinking water clinically.
引文
[1]唐嘉,谢佳,李中文,等.热应激对鸡免疫系统的影响及预防研究进展[J].贵州农业科学,2011,39(12):170-173.Tang J,Xie J,Li W W,et al. Research progress on the effects of heat stress on chicken immune system and prevention[J].Guizhou Agricultural Science,2011,39(12):170-173.
[2]董圣洁,邓雯.热应激对鸡生物学特性的影响[J].中国兽医杂志,2012,48(9):83-86.Dong Sh J,Deng W. Effects of heat stress on the biological characteristics of chickens[J]. Chinese Journal of Veterinary Medicine,2012,48(9):83-86.
[3]肖敏华,石达友,代雪立,等.复方中药对慢性热应激下蛋鸡生产性能和心脏组织HSP70表达量的影响[J].中国家禽,2011,32(21):14-16.Xiao M H,Shi D Y,Dai X L,et al. Effects of compound Chinese medicine on production performance and HSP70 expression in heart tissue of laying hens under chronic heat stress[J].Chinese Poultry,2011,32(21):14-16.
[4]农业部兽药兽药评审中心.兽药研究技术指导原则汇编(2006-2011年)[M].北京:化学工业出版社,2013,192-196.Center for Veterinary Drug and Veterinary Drug Assessment,Ministry of Agriculture. Compilation of Guiding Principles for Veterinary Drug Research Technology(2006-2011)[M]. Beijing:Chemical Industry Press,2013,192-196.
[5]张云波,梁园,夏爱军.柴胡解热作用的药理研究进展[J].中国药业,2011,20(3):79-80.Zhang Y B,Liang Y,Xia A J. Advances in pharmacological studies on antipyretic effects of Bupleurum chinense[J]. Chinese Pharmaceutical Industry,2011,20(3):79-80.
[6]黄芩的化学成分与药理作用研究进展[J].中成药,2016,38(1):141-147.Advances in studies on chemical constituents and pharmacological effects of Scutellaria baicalensis[J]. Chinese patent medicines,2016,38(1):141-147.
[7]吉晓丽.黄芩的化学成分与药理作用研究进展[J].中医临床研究,2017,9(9):128-129.Ji X L. Advances in studies on chemical constituents and pharmacological effects of Scutellaria baicalensis[J]. Clinical studies of traditional Chinese medicine,2017,9(9):128-129
[8]胡煜明,李顺祥,严建业,等.柴胡、黄芩药对的研究进展[J].湖南中医杂志,2014,30(7):199-201.Hu Y M,Li Sh X,Yan J Y,et al. Research progress of Bupleurum chinense and Scutellaria baicalensis pairs[J]. Hunan Journal of Traditional Chinese Medicine,2014,30(7):199-201.
[9]李亚芹,瞿融.柴胡和黄芩配伍的研究进展[J].中国中医基础医学杂志,2018(2).Li YQ,Qu R. Research progress of compatibility of Bupleurum chinense and Scutellaria baicalensis[J]. Chinese Journal of Basic Medicine of Traditional Chinese Medicine,2018(2).
[10]杨江萍.柴胡-黄芩药对退热有效部位及其配伍作用机制的研究[D].北京中医药大学,2012.Yang J P. Study on the antipyretic effective components and their compatibility mechanism of bupleurum Chinense and scutellaria baicalensis[D]. Beijing University of Traditional Chinese Medicine,2012.
[2]董圣洁,邓雯.热应激对鸡生物学特性的影响[J].中国兽医杂志,2012,48(9):83-86.Dong Sh J,Deng W. Effects of heat stress on the biological characteristics of chickens[J]. Chinese Journal of Veterinary Medicine,2012,48(9):83-86.
[3]肖敏华,石达友,代雪立,等.复方中药对慢性热应激下蛋鸡生产性能和心脏组织HSP70表达量的影响[J].中国家禽,2011,32(21):14-16.Xiao M H,Shi D Y,Dai X L,et al. Effects of compound Chinese medicine on production performance and HSP70 expression in heart tissue of laying hens under chronic heat stress[J].Chinese Poultry,2011,32(21):14-16.
[4]农业部兽药兽药评审中心.兽药研究技术指导原则汇编(2006-2011年)[M].北京:化学工业出版社,2013,192-196.Center for Veterinary Drug and Veterinary Drug Assessment,Ministry of Agriculture. Compilation of Guiding Principles for Veterinary Drug Research Technology(2006-2011)[M]. Beijing:Chemical Industry Press,2013,192-196.
[5]张云波,梁园,夏爱军.柴胡解热作用的药理研究进展[J].中国药业,2011,20(3):79-80.Zhang Y B,Liang Y,Xia A J. Advances in pharmacological studies on antipyretic effects of Bupleurum chinense[J]. Chinese Pharmaceutical Industry,2011,20(3):79-80.
[6]黄芩的化学成分与药理作用研究进展[J].中成药,2016,38(1):141-147.Advances in studies on chemical constituents and pharmacological effects of Scutellaria baicalensis[J]. Chinese patent medicines,2016,38(1):141-147.
[7]吉晓丽.黄芩的化学成分与药理作用研究进展[J].中医临床研究,2017,9(9):128-129.Ji X L. Advances in studies on chemical constituents and pharmacological effects of Scutellaria baicalensis[J]. Clinical studies of traditional Chinese medicine,2017,9(9):128-129
[8]胡煜明,李顺祥,严建业,等.柴胡、黄芩药对的研究进展[J].湖南中医杂志,2014,30(7):199-201.Hu Y M,Li Sh X,Yan J Y,et al. Research progress of Bupleurum chinense and Scutellaria baicalensis pairs[J]. Hunan Journal of Traditional Chinese Medicine,2014,30(7):199-201.
[9]李亚芹,瞿融.柴胡和黄芩配伍的研究进展[J].中国中医基础医学杂志,2018(2).Li YQ,Qu R. Research progress of compatibility of Bupleurum chinense and Scutellaria baicalensis[J]. Chinese Journal of Basic Medicine of Traditional Chinese Medicine,2018(2).
[10]杨江萍.柴胡-黄芩药对退热有效部位及其配伍作用机制的研究[D].北京中医药大学,2012.Yang J P. Study on the antipyretic effective components and their compatibility mechanism of bupleurum Chinense and scutellaria baicalensis[D]. Beijing University of Traditional Chinese Medicine,2012.
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