SHCBP1基因在结肠癌组织中表达及对结肠癌细胞增殖迁移的影响
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摘要
目的:探讨SHCBP1基因在结肠癌组织中的表达情况及结肠癌进展过程中的作用。方法:荧光定量PCR、免疫组织化学法检测SHCBP1基因在57例临床结肠癌样本的癌及癌旁组织中表达情况、SHCBP1蛋白表达与定位;构建SHCBP1过表达质粒及合成特异性靶向SHCBP1的siRNA、shRNA并转染结肠癌细胞株,Real-time PCR、Western blot检测细胞株SHCBP1 mRNA和蛋白的表达量,CCK8法、克隆形成检测SHCBP1对结肠癌细胞株增殖能力的影响;划痕实验、Transwell实验检测过表达、干扰后SHCBP1对细胞迁移能力的影响。结果:在57对样本中,结肠癌组织中SHCBP1的表达高于癌旁组织(P <0. 001); SHCBP1主要表达于结肠癌细胞的胞质中。过表达SHCBP1后可显著促进结肠癌细胞株增殖迁移,干扰SHCBP1可抑制结肠癌细胞株增殖迁移。结论:SHCBP1在结肠癌组织中起到促进细胞增殖迁移的作用,在结肠癌的发生发展起到促进作用。
Objective: To investigate the expression of SHCBP1 in colon cancer tissues,and evaluate the role of SHCBP1 in proliferation and migration of human colon cancer cell line. Methods: Real-time PCR,immunohistochemistry and Western blot analysis were used to detect the expression of SHCBP1 in 57 pairs of colon cancer tissues and corresponding adjacent tissue. Cellular growth curve,colony formation,cell wound healing and Transwell were used to examine the effect of SHCBP1 overexpression or knockdown on proliferation and migration. Results: The expressions of SHCBP1 mRNA level and protein had evident individual differences among colon cancer tissues compared with the adjacent tissues by RT-PCR,Western blot analysis( P < 0. 001). Overexpression of SHCBP1 significant promoted cell proliferation,colony formation and migration in human colon cancer cell. Meanwhile,SHCBP1 silence resulted in significant decrease. Conclusion: HCBP1 can promote cell proliferation,migration,and may play a promoting role in the development of colon cancer.
Objective: To investigate the expression of SHCBP1 in colon cancer tissues,and evaluate the role of SHCBP1 in proliferation and migration of human colon cancer cell line. Methods: Real-time PCR,immunohistochemistry and Western blot analysis were used to detect the expression of SHCBP1 in 57 pairs of colon cancer tissues and corresponding adjacent tissue. Cellular growth curve,colony formation,cell wound healing and Transwell were used to examine the effect of SHCBP1 overexpression or knockdown on proliferation and migration. Results: The expressions of SHCBP1 mRNA level and protein had evident individual differences among colon cancer tissues compared with the adjacent tissues by RT-PCR,Western blot analysis( P < 0. 001). Overexpression of SHCBP1 significant promoted cell proliferation,colony formation and migration in human colon cancer cell. Meanwhile,SHCBP1 silence resulted in significant decrease. Conclusion: HCBP1 can promote cell proliferation,migration,and may play a promoting role in the development of colon cancer.
引文
[1] Wills MK,Keyvani CA,Lau HR,et al. Signalling adaptor ShcD suppresses erk phosphorylation distal to the ret and trk neurotrophic receptors[J]. Journal of Biological Chemistry,2017,292(14):5748.
[2] Buckley MW,S Arandjelovic,PC Trampont,et al. Unexpected phenotype of mice lacking Shcbp1,a protein induced during T cell proliferation[J]. PLo S One,2014,9(8):e105576.
[3] Schmandt R,SK Liu,CJ Mcglade. Cloning and characterization of m PAL,a novel Shc SH2 domain-binding protein expressed in proliferating cells[J]. Oncogene,1999,18(10):1867-1879.
[4] Asano E,H Hasegawa,T Hyodo,et al. SHCBP1 is required for midbody organization and cytokinesis completion[J]. Cell Cycle,2014,13(17):2744-2751.
[5] Buckley MW,Arandjelovic S,Trampont PC,et al. Unexpected phenotype of mice lacking shcbp1,a protein induced during T cell proliferation[J]. PLo S One,2014,9(8):105576.
[6] Ito M,Iwasaki M,Takeda M,et al. Measles virus nonstructural c protein modulates viral RNA polymerase activity by interacting with host protein SHCBP1[J]. Journal of Virology,2013,87(17):9633-9642.
[7] Zhou Y,Tan Z,Chen K,et al. Overexpression of SHCBP1 promotes migration and invasion in gliomas by activating the NF-κB signaling pathway[J]. Molecular Carcinogenesis,2018,57(9):1181-1196.
[8] Peng C,Zhao H,Chen W,et al. Identification of SHCBP1 as a novel downstream target gene of SS18-SSX1 and its functional analysis in progression of synovial sarcoma[J]. Oncotarget,2016,7(41):66822-66834.
[9] Colak D,A Nofal,A Albakheet,et al. Age-specific gene expression signatures for breast tumors and cross-species conserved potential cancer progression markers in young women[J]. PLo S One,2013,8(5):e63204.
[10] Feng W,HC Li,K Xu,et al. SHCBP1 is over-expressed in breast cancer and is important in the proliferation and apoptosis of the human malignant breast cancer cell line[J]. Gene,2016,587(1):91-97.
[11] Tao HC,HX Wang,M Dai,et al. Targeting SHCBP1 inhibits cell proliferation in human hepatocellular carcinoma cells[J]. Asian Pac J Cancer Prev,2013,14(10):5645-5650.
[12] Lei L,Yi Y,Shihu AL,et al. EGF-induced nuclear localization of SHCBP1 activatesβ-catenin signaling and promotes cancer progression[J]. Oncogene,2018,21(9):2107-2114.
[13] Peng C,H Zhao,Y Song,et al. SHCBP1 promotes synovial sarcoma cell metastasis via targeting TGF-beta1/Smad signaling pathway and is associated with poor prognosis[J]. J Exp Clin Cancer Res,2017,36(1):141.
[14] Wu B,Chen M,Gao M,et al. Down-regulation of lnc TCF7 inhibits cell migration and invasion in colorectal cancer via inhibiting TCF7 expression[J]. Human Cell,2019,32(1):31-40.
[15] Matsuoka H,Morise Z,Tanaka C,et al. Repeat hepatectomy with systemic chemotherapy might improve survival of recurrent liver metastasis from colorectal cancer—a retrospective observational study[J]. World Journal of Surgical Oncology,2019,17(1):33.
[2] Buckley MW,S Arandjelovic,PC Trampont,et al. Unexpected phenotype of mice lacking Shcbp1,a protein induced during T cell proliferation[J]. PLo S One,2014,9(8):e105576.
[3] Schmandt R,SK Liu,CJ Mcglade. Cloning and characterization of m PAL,a novel Shc SH2 domain-binding protein expressed in proliferating cells[J]. Oncogene,1999,18(10):1867-1879.
[4] Asano E,H Hasegawa,T Hyodo,et al. SHCBP1 is required for midbody organization and cytokinesis completion[J]. Cell Cycle,2014,13(17):2744-2751.
[5] Buckley MW,Arandjelovic S,Trampont PC,et al. Unexpected phenotype of mice lacking shcbp1,a protein induced during T cell proliferation[J]. PLo S One,2014,9(8):105576.
[6] Ito M,Iwasaki M,Takeda M,et al. Measles virus nonstructural c protein modulates viral RNA polymerase activity by interacting with host protein SHCBP1[J]. Journal of Virology,2013,87(17):9633-9642.
[7] Zhou Y,Tan Z,Chen K,et al. Overexpression of SHCBP1 promotes migration and invasion in gliomas by activating the NF-κB signaling pathway[J]. Molecular Carcinogenesis,2018,57(9):1181-1196.
[8] Peng C,Zhao H,Chen W,et al. Identification of SHCBP1 as a novel downstream target gene of SS18-SSX1 and its functional analysis in progression of synovial sarcoma[J]. Oncotarget,2016,7(41):66822-66834.
[9] Colak D,A Nofal,A Albakheet,et al. Age-specific gene expression signatures for breast tumors and cross-species conserved potential cancer progression markers in young women[J]. PLo S One,2013,8(5):e63204.
[10] Feng W,HC Li,K Xu,et al. SHCBP1 is over-expressed in breast cancer and is important in the proliferation and apoptosis of the human malignant breast cancer cell line[J]. Gene,2016,587(1):91-97.
[11] Tao HC,HX Wang,M Dai,et al. Targeting SHCBP1 inhibits cell proliferation in human hepatocellular carcinoma cells[J]. Asian Pac J Cancer Prev,2013,14(10):5645-5650.
[12] Lei L,Yi Y,Shihu AL,et al. EGF-induced nuclear localization of SHCBP1 activatesβ-catenin signaling and promotes cancer progression[J]. Oncogene,2018,21(9):2107-2114.
[13] Peng C,H Zhao,Y Song,et al. SHCBP1 promotes synovial sarcoma cell metastasis via targeting TGF-beta1/Smad signaling pathway and is associated with poor prognosis[J]. J Exp Clin Cancer Res,2017,36(1):141.
[14] Wu B,Chen M,Gao M,et al. Down-regulation of lnc TCF7 inhibits cell migration and invasion in colorectal cancer via inhibiting TCF7 expression[J]. Human Cell,2019,32(1):31-40.
[15] Matsuoka H,Morise Z,Tanaka C,et al. Repeat hepatectomy with systemic chemotherapy might improve survival of recurrent liver metastasis from colorectal cancer—a retrospective observational study[J]. World Journal of Surgical Oncology,2019,17(1):33.
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