安神定志汤对实验性大鼠心律失常的作用

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英文篇名：Effect of Anshen Dingzhi Decoction on Experimental Arrhythmia in Rats 作者：李亚萍 ; 张明昊 ; 章金涛 英文作者：LI Yaping;ZHANG Minghao;ZHANG Jintao;Chinese Medicine Hospital of Zhengzhou;Henan University of Chinese Medicine;Institute of Medicine,Zhengzhou University; 关键词：安神定志汤 ; 心律失常 ; 乌头碱 ; 氯化钡 ; 肾上腺素 ; 大鼠 英文关键词：Anshen Dingzhi Decoction;;arrhythmia;;aconitine;;barium chloride;;adrenaline;;rat 中文刊名：HNZK 英文刊名：Acta Chinese Medicine 机构：郑州市中医院;河南中医药大学;郑州大学医药科学研究院; 出版日期：2019-04-30 20:40 出版单位：中医学报 年：2019 期：v.34;No.252 基金：国家自然科学基金项目(31372270);; 河南中医药大学科研苗圃工程项目(MP2016-04) 语种：中文; 页：HNZK201905016 页数：5 CN：05 ISSN：41-1411/R 分类号：70-74

摘要

目的:观察安神定志汤对乌头碱、氯化钡和肾上腺素诱导的实验性心律失常的对抗作用以及对血清Ca~(2+)、乳酸脱氢酶(lactate dehydrogenase,LDH)水平的影响。方法:70只SD大鼠随机分为乌头碱诱发心律失常模型组、乌头碱诱发心律失常模型并安神定志汤治疗组,氯化钡诱发心律失常模型组、氯化钡诱发心律失常模型并安神定志汤治疗组,肾上腺素诱发心律失常模型组、肾上腺素诱发心律失常模型并安神定志汤治疗组及正常对照组,每组10只。除正常对照组外,其余各组大鼠分别采用舌下静脉注射乌头碱、氯化钡和肾上腺素构建大鼠心律失常的3种动物模型,治疗组灌胃给予安神定志汤,以BL-420生物机能实验系统记录分析心电图曲线,并取血检测大鼠血清Ca~(2+)、LDH水平。结果:与乌头碱诱发心律失常模型组比较,乌头碱诱发心律失常模型并安神定志汤治疗组大鼠相应体质量累积乌头碱用量显著降低(P<0.05);与氯化钡诱发心律失常模型组比较,氯化钡诱发心律失常模型并安神定志汤治疗组大鼠心律失常发生时间显著推迟(P<0.05),心律失常的持续时间也显著缩短(P<0.05);与肾上腺素诱发心律失常模型组比较,肾上腺素诱发心律失常模型并安神定志汤治疗组心律失常发生时间显著推迟(P<0.05),心律失常的持续时间也显著缩短(P<0.05)。与正常对照组比较,乌头碱诱发心律失常模型组、氯化钡诱发心律失常模型组和肾上腺素诱发心律失常模型组大鼠血清中Ca~(2+)、LDH水平明显升高(P<0.01)。而乌头碱诱发心律失常模型并安神定志汤治疗组、氯化钡诱发心律失常模型并安神定志汤治疗组和肾上腺素诱发心律失常模型并安神定志汤治疗组大鼠分别与相应模型组比较,血清中Ca~(2+)、LDH水平明显降低(P<0.01)。结论:安神定志汤可以通过降低血清Ca~(2+)、LDH水平来发挥对乌头碱、氯化钡和肾上腺素诱导的实验性心律失常的对抗作用。
        Objective:To observe the antagonistic effect of Anshen Dingzhi Decoction on experimental arrhythmias induced by aconitine,barium chloride and adrenaline,and the effects on serum Ca~(2+) and lactate dehydrogenase(LDH) levels.Methods:70 SD rats were randomly divided into aconitine-induced arrhythmia model group,aconitine-induced arrhythmia model and Anshen Dingzhi Decoction treatment group,barium chloride-induced arrhythmia model group,barium chloride-induced arrhythmia model and Anshen Dingzhi decoction treatment group,adrenaline-induced arrhythmia model group,adrenaline-induced arrhythmia model and Anshen Dingzhi decoction treatment group and normal control group,10 in each group.Except for the normal control group,rats in the other groups were sublingual intravenous injection of aconitine,barium chloride and epinephrine to construct arrhythmia models in rats.The treatment group was given Anshen Dingzhi Decoction by gavage.The ECG curve was recorded and analyzed by BL-420 biological function experimental system,and the serum Ca~(2+),LDH levels were detected by blood sampling.Results:Compared with aconitine-induced arrhythmia model group,aconitine-induced arrhythmia model group and Anshen Dingzhi Decoction treatment group significantly reduced the cumulative amount of aconitine(P<0.05).Compared with barium chloride induced arrhythmia model group,barium chloride induced arrhythmia model and Anshen Dingzhi decoction treatment group significantly delayed the occurrence of arrhythmia(P<0.05),and the duration of arrhythmia was significantly shortened(P<0.05).Compared with the adrenaline-induced arrhythmia model group,the time of arrhythmia in the adrenaline-induced arrhythmia model and Anshen Dingzhi decoction treatment group was significantly delayed(P<0.05),and the duration of arrhythmia was significantly shortened(P<0.05).Compared with the normal control group,the serum levels of Ca~(2+),LDH in aconitine-induced arrhythmia model group,barium chloride-induced arrhythmia model group and epinephrine-induced arrhythmia model group were significantly higher(P<0.01).The levels of Ca~(2+),LDH in serum of rats in aconitine-induced arrhythmia model and Anshen Dingzhi Decoction treatment group,barium chloride-induced arrhythmia model and Anshen Dingzhi decoction treatment group and adrenaline-induced arrhythmia model and Anshen Dingzhi Decoction treatment group were significantly lower than those in Wu corresponding model group(P<0.01).Conclusion:Anshen Dingzhi Decoction can antagonize experimental arrhythmia induced by aconitine,barium chloride and epinephrine by reducing serum Ca~(2+),LDH levels.

引文

[1]张玮,叶治国,崔洁.杭白菊乙酸乙酯提取物对大鼠实验性心律失常的影响及其机制[J].浙江大学学报(医学版),2009,38(4):377-382.
    [2]何晓山,代蓉,陈秀红,等.滇黄芩总黄酮急性毒性及抗实验性心律失常作用的研究[J].中国实验方剂学杂志,2010,16(10):150-152.
    [3]尹克春,刘淑娟,陈力,等.当归颗粒对抗氯化钙引起实验性心律失常的作用[J].广东医学,2008,29(11):1785-1786.
    [4]程国彭.医学心悟[M].北京:人民卫生出版社,2006:161.
    [5]朱晨军,唐启盛.安神定志丸治疗心胆气虚型抑郁症的临床疗效观察[J].中国实验方剂学杂志,2010,16(5):206-208.
    [6]辜炜君.安神定志汤结合抗焦虑药治疗广泛性焦虑障碍的临床效果[J].黑龙江医学,2016,40(11):1024-1025.
    [7]郭力,李彧,赵福建,等.稳心颗粒抗实验性大鼠心律失常作用的研究[J].北京中医药大学学报,2013,36(7):472-475.
    [8]谢安,戴益民,朱妙草.细胞外Ca2+对双基因内向整流钾通道的阻断作用[J].心脏,2000,12(5):345.
    [9]张元沛.药理学实验[M].2版.北京:人民卫生出版社,1996:68.
    [10]张梦玺,关瑜,袁洪,等.心律失常患者血清和淋巴细胞中钙、镁的分布[J].湖南医科大学学报,1998,23(1):82-84.
    [11]程臻,龚一萍.复脉汤对心肌缺血后心律失常大鼠干预作用的实验研究[J].山西中医学院学报,2014,15(3):20-23.
    [12]刘珊,滕佳林,王永春.附子正丁醇、水提取物对虚寒证模型大鼠琥珀酸脱氢酶、乳酸脱氢酶活性的影响[J].时珍国医国药,2014,25(8):1839-1841.