刘丽教授气滞血瘀型输卵管炎造模方法总结
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摘要
导师刘丽教授师从于龙江韩氏妇科,从医近30余年,擅长中西医结合治疗气滞血瘀型输卵管炎性不孕,经过多年理论研究与临床实践,根据病证结合理论,对气滞血瘀型输卵管炎模型建立颇有心得。导师造模方法为先用金黄色葡萄球菌注入到输卵管中建立疾病模型,然后采用声、光、电等复合因素建立证型模型。目前中医研究疾病模型,大多很难病证相结合,文章通过对相关文献进行了总结论述,以期为同一疾病同一证候建立一个统一的造模方法和一个统一的评价体系。中医药从业人员,需要多角度、深入进行国际交流,不断完善自己的方法。
Professor Liu Li learns from Yu Longjiang,under the mentoring relationship of Han's gynecology. Prof.Liu has been engaged in medicine activity for over 30 years and is good at the treatment of salpingitis-induced infertility of qi and blood stagnation pattern with the integrative Chinese and western medicine. Based on the integration of symptoms and theory,Prof. Liu has accumulated the experiences in preparation of model salpingitis. Firstly,staphylococcus aureus is injected into fallopian tube to build up disease model. Using complex factors,e. g. sound,light and electricity,the pattern model is prepared. Currently,in the preparation of disease model for the TCM research,it is very difficult to achieve the integration of disease and TCM pattern. In the paper,through reviewing relevant literatures,it is anticipated to build a compound model with both disease and TCM pattern involved and achieve systematic review. The employees of TCM need to improve the research methods from various views to perform further international exchange.
Professor Liu Li learns from Yu Longjiang,under the mentoring relationship of Han's gynecology. Prof.Liu has been engaged in medicine activity for over 30 years and is good at the treatment of salpingitis-induced infertility of qi and blood stagnation pattern with the integrative Chinese and western medicine. Based on the integration of symptoms and theory,Prof. Liu has accumulated the experiences in preparation of model salpingitis. Firstly,staphylococcus aureus is injected into fallopian tube to build up disease model. Using complex factors,e. g. sound,light and electricity,the pattern model is prepared. Currently,in the preparation of disease model for the TCM research,it is very difficult to achieve the integration of disease and TCM pattern. In the paper,through reviewing relevant literatures,it is anticipated to build a compound model with both disease and TCM pattern involved and achieve systematic review. The employees of TCM need to improve the research methods from various views to perform further international exchange.
引文
[1]Kjersgaard C,Arendt LH,Ernst A,et al. Lifestyle in pregnancy and cryptorchidism in sons:a study within two large Danish birth cohorts[J]. Clinical Epidemiology,2018,19(10):311-322.
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[7]鲁兴隆.肺抑瘤膏治疗非小细胞肺癌气滞血瘀证临床观察与实验研究[D].济南:山东中医药大学,2015.
[8]Ponomarev ED. Fresh Evidence for Platelets as Neuronal and Innate Immune Cells:Their Role in the Activation,Differentiation,and Deactivation of Th1,Th17,and Tregs during Tissue Inflammation[J].Front Immunol,2018(9):406.
[9]王婷婷,李世大,白椿宇,等.气滞血瘀型输卵管炎性不孕症大鼠模型的建立[J].世界中西医结合杂志,2017,12(12):1691-1694.
[10]徐雨静,赵瑞芝,胡巧红.莪术对甲氨蝶呤在正常和气滞血瘀大鼠体内药动学的影响[J].广东药学院学报,2016,32(4):403-405.
[11]Zhang Y,Shao L,Li X,et al. Uterotubal junction prevents chlamydial ascension via innate immunity[J]. PLo S ONE,2017,12(8):e0183189.
[12]刘丽,李杨,李世大.膈下逐瘀冲剂对沙眼衣原体输卵管炎性不孕小鼠细胞因子影响的研究[J].光明中医,2016,31(22):3268-3270.
[13]刘丽,李世大,赵丽燕.膈下逐瘀冲剂对输卵管炎性不孕大鼠NF-κB、IFN-γ蛋白表达的影响[J].世界中西医结合杂志,2014,9(3):251-253,271.
[14]Li Z,Zhang Z,Ming WK,et al. Tracing GFP-labeled WJMSCs in vivo using a chronic salpingitis model:an animal experiment[J].Stem Cell Res Ther,2017,8(1):272.
[15]蔡思琪,李沅衡,黎嘉,等.鼠类行为学实验方法研究进展[J].医学综述,2018,24(5):916-920.
[16]Eom JS,Kim I,Kim WY,et al. Household tuberculosis contact investigation in a tuberculosis-prevalent country:Are the tuberculin skin test and interferon-gamma release assay enough in elderly contacts?[J]. Medicine(Baltimore),2018,97(3):e9681.
[17]Liu Y,Perez J,Hammer LA,et al. Intravaginal Administration of Interleukin 12 during Genital Gonococcal Infection in Mice Induces Immunity to Heterologous Strains of Neisseria gonorrhoeae[J].m Sphere,2018,3(1):e00421-17.
[18]郑跃,李世大,李杨,等.“活血化瘀、行气止痛”大法对输卵管炎性不孕大鼠模型中TLR4受体信号转导通路作用机理的研究[J].中医临床研究,2016,8(23):16-18.
[19]Lentz SAM,Rivas PM,Cardoso MRI,et al. Bacillus cereus as the main casual agent of foodborne outbreaks in Southern Brazil:data from 11 years[J]. Cad Saúde Pública,2018,34(4),e00057417.
[20]Kularatne RS,Muller EE,Maseko DV,et al. Trends in the relative prevalence of genital ulcer disease pathogens and association with HIV infection in Johannesburg,South Africa,2007-2015[J]. PLo S ONE,2018,13(4):e0194125.
[21]Durand G,Javerliat F,Bes M,et al. Routine Whole-Genome Sequencing for Outbreak Investigations of Staphylococcus aureus in a National Reference Center[J]. Front Microbiol,2018(9):511.
[22]Bureta C,Tominaga H,Yamamoto T,et al. Lumbar spine epidural abscess and facet joint septic arthritis due to Streptococcus agalactiae:a case report[J]. BMC Surg,2018,18(1):16.
[23]Kolter J,Henneke P. Codevelopment of Microbiota and Innate Immunity and the Risk for Group B Streptococcal Disease[J]. Frontiers Immunology,2017(8):1497.
[24]覃梅春,陈奔,钟振国.阿尔茨海默病-血瘀证病证结合树鼩模型稳定性研究[J].亚太传统医药,2017,13(16):17-19.
[25]陈燕清,杨晶晶,曹卓青,等.病证结合动物模型的思考[J].中国中医基础医学杂志,2017,23(5):628-629,705.
[2]Cerny KL,Van Fleet M,Slepenkin A,et al. Differential Expression of mRNA Encoding Cytokines and Chemokines in the Reproductive Tract after Infection of Mice with Chlamydia trachomatis[J]. Repro Syst Sexual Disord,2015,4(3):152.
[3]Graspeuntner S,Bohlmann MK,Gillmann K,et al. Microbiota-based analysis reveals specific bacterial traits and a novel strategy for the diagnosis of infectious infertility[J]. PLo S ONE,2018,13(1):e0191047.
[4]乔杰,龙晓宇,高江曼,等.人类生育力保护的机遇与挑战[J].中国实用妇科与产科杂志,2016,32(1):8-12.
[5]邓天琦.输卵管炎性不孕症临床分型、用药规律分析及实验观察[D].哈尔滨:黑龙江中医药大学,2017.
[6]任宏义,任周新.“气滞血瘀”证动物模型的建立[J].北京实验动物科学与管理,1994(3):29-31.
[7]鲁兴隆.肺抑瘤膏治疗非小细胞肺癌气滞血瘀证临床观察与实验研究[D].济南:山东中医药大学,2015.
[8]Ponomarev ED. Fresh Evidence for Platelets as Neuronal and Innate Immune Cells:Their Role in the Activation,Differentiation,and Deactivation of Th1,Th17,and Tregs during Tissue Inflammation[J].Front Immunol,2018(9):406.
[9]王婷婷,李世大,白椿宇,等.气滞血瘀型输卵管炎性不孕症大鼠模型的建立[J].世界中西医结合杂志,2017,12(12):1691-1694.
[10]徐雨静,赵瑞芝,胡巧红.莪术对甲氨蝶呤在正常和气滞血瘀大鼠体内药动学的影响[J].广东药学院学报,2016,32(4):403-405.
[11]Zhang Y,Shao L,Li X,et al. Uterotubal junction prevents chlamydial ascension via innate immunity[J]. PLo S ONE,2017,12(8):e0183189.
[12]刘丽,李杨,李世大.膈下逐瘀冲剂对沙眼衣原体输卵管炎性不孕小鼠细胞因子影响的研究[J].光明中医,2016,31(22):3268-3270.
[13]刘丽,李世大,赵丽燕.膈下逐瘀冲剂对输卵管炎性不孕大鼠NF-κB、IFN-γ蛋白表达的影响[J].世界中西医结合杂志,2014,9(3):251-253,271.
[14]Li Z,Zhang Z,Ming WK,et al. Tracing GFP-labeled WJMSCs in vivo using a chronic salpingitis model:an animal experiment[J].Stem Cell Res Ther,2017,8(1):272.
[15]蔡思琪,李沅衡,黎嘉,等.鼠类行为学实验方法研究进展[J].医学综述,2018,24(5):916-920.
[16]Eom JS,Kim I,Kim WY,et al. Household tuberculosis contact investigation in a tuberculosis-prevalent country:Are the tuberculin skin test and interferon-gamma release assay enough in elderly contacts?[J]. Medicine(Baltimore),2018,97(3):e9681.
[17]Liu Y,Perez J,Hammer LA,et al. Intravaginal Administration of Interleukin 12 during Genital Gonococcal Infection in Mice Induces Immunity to Heterologous Strains of Neisseria gonorrhoeae[J].m Sphere,2018,3(1):e00421-17.
[18]郑跃,李世大,李杨,等.“活血化瘀、行气止痛”大法对输卵管炎性不孕大鼠模型中TLR4受体信号转导通路作用机理的研究[J].中医临床研究,2016,8(23):16-18.
[19]Lentz SAM,Rivas PM,Cardoso MRI,et al. Bacillus cereus as the main casual agent of foodborne outbreaks in Southern Brazil:data from 11 years[J]. Cad Saúde Pública,2018,34(4),e00057417.
[20]Kularatne RS,Muller EE,Maseko DV,et al. Trends in the relative prevalence of genital ulcer disease pathogens and association with HIV infection in Johannesburg,South Africa,2007-2015[J]. PLo S ONE,2018,13(4):e0194125.
[21]Durand G,Javerliat F,Bes M,et al. Routine Whole-Genome Sequencing for Outbreak Investigations of Staphylococcus aureus in a National Reference Center[J]. Front Microbiol,2018(9):511.
[22]Bureta C,Tominaga H,Yamamoto T,et al. Lumbar spine epidural abscess and facet joint septic arthritis due to Streptococcus agalactiae:a case report[J]. BMC Surg,2018,18(1):16.
[23]Kolter J,Henneke P. Codevelopment of Microbiota and Innate Immunity and the Risk for Group B Streptococcal Disease[J]. Frontiers Immunology,2017(8):1497.
[24]覃梅春,陈奔,钟振国.阿尔茨海默病-血瘀证病证结合树鼩模型稳定性研究[J].亚太传统医药,2017,13(16):17-19.
[25]陈燕清,杨晶晶,曹卓青,等.病证结合动物模型的思考[J].中国中医基础医学杂志,2017,23(5):628-629,705.
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