光质对三叶崖爬藤光合特性和总黄酮含量调控的研究
|
摘要
本文主要目的是研究光质对三叶崖爬藤(Tetrastigma hemsleyanum)光合特性[叶绿素含量、净光合速率(P_n)、气孔导度(G_s)、蒸腾速率(T_r)、胞间CO_2浓度(C_i)]及总黄酮含量的影响。以二年生已初生块根的三叶崖爬藤扦插苗为处理材料,种植在透明膜(CK-W)、红膜(RF)、黄膜(YF)、绿膜(GF)及蓝膜(BF)覆盖的大棚中。结果表明:RF处理下其叶绿素a(Chl a)、叶绿素b(Chl b)和叶绿素a+b(Chl a+b)含量最高,BF处理下叶绿素a/b(Chl a/b)极显著高于其他处理;光合-光响应曲线拟合后发现,与CK-W相比,YF、BF和GF处理下三叶崖爬藤P_n、G_s、T_r和C_i均较高,RF处理下最低。全天光合测定表明,三叶崖爬藤具有光合"午休"现象,其中CK-W与BF处理下P_n最高且相近,RF整体处于较低水平;T_r与C_i在日变化过程中大致与G_s呈同步变化趋势。CK-W和YF处理下的叶片中的总黄酮含量极显著高于其他光质处理,RF处理下块根中的总黄酮含量最高且与其他光质处理差异极显著。综上所述,三叶崖爬藤的光合特性和不同部位的总黄酮含量对光质处理的响应都是有所差异的。
To uncover the effect of light quality on photosynthetic characteristics[chlorophyll(Chl)content,net photosynthetic rate(P_n),stomatal conductance(G_s),transpiration rate(T_r)and intercellular CO_2 concentration(C_i)]and total flavonoid content in Tetrastigma hemsleyanum,two-year-old cutting seedlings with primary root tubers were investigated under white plastic film(CK-W),red plastic film(RF),yellow plastic film(YF),green plastic film(GF)and blue plastic film(BF),respectively.The highest contents of Chl a,Chl b,and Chl a+b in T.hemsleyanum were detected under RF,and the Chl a/b under BF was significantly higher than those under other films.Compared to CK-W treatment,P_n,G_s,T_r and C_i were markedly higher in YF,BF and GF treatments,but were lowest in RF treatment.A"midday break"phenomenon was found in T.hemsleyanum during diurnal photosynthesis.P_n was the highest in CK-W and BF treatments with similar variation trends,while was lower under RF.T_r and C_i showed synchronous trends with G_s in diurnal variation.Under CK-W and YF,the total flavonoid contents in leaves were significantly higher than those in other treatments,while the highest content in roots was detected under RF with significantly difference in contrast to other treatments.Therefore,photosynthetic characteristics and total flavonoid content in different parts of T.hemsleyanum showed different responses to light quality.
To uncover the effect of light quality on photosynthetic characteristics[chlorophyll(Chl)content,net photosynthetic rate(P_n),stomatal conductance(G_s),transpiration rate(T_r)and intercellular CO_2 concentration(C_i)]and total flavonoid content in Tetrastigma hemsleyanum,two-year-old cutting seedlings with primary root tubers were investigated under white plastic film(CK-W),red plastic film(RF),yellow plastic film(YF),green plastic film(GF)and blue plastic film(BF),respectively.The highest contents of Chl a,Chl b,and Chl a+b in T.hemsleyanum were detected under RF,and the Chl a/b under BF was significantly higher than those under other films.Compared to CK-W treatment,P_n,G_s,T_r and C_i were markedly higher in YF,BF and GF treatments,but were lowest in RF treatment.A"midday break"phenomenon was found in T.hemsleyanum during diurnal photosynthesis.P_n was the highest in CK-W and BF treatments with similar variation trends,while was lower under RF.T_r and C_i showed synchronous trends with G_s in diurnal variation.Under CK-W and YF,the total flavonoid contents in leaves were significantly higher than those in other treatments,while the highest content in roots was detected under RF with significantly difference in contrast to other treatments.Therefore,photosynthetic characteristics and total flavonoid content in different parts of T.hemsleyanum showed different responses to light quality.
引文
Chen GY,Chen J,Xu DQ(2010).Thinking about the relationship between net photosynthetic rate and inter-cellular CO2 concentration.Plant Physiol Commun,46(1):64-66(in Chinese)[陈根云,陈娟,许大全(2010).关于净光合速率和胞间CO2浓度关系的思考.植物生理学通讯,46(1):64-66]
Chen M,Wu L,Cao L,et al(2016).Effect of light quality on growth and main chemical composition of tissue culture in Anoectochilus Roxburghii.China Illum Eng J,27(2):112-117(in Chinese with English abstract)[陈美香,武礼宾,曹立等(2016).光质对金线莲组培苗生长和主要化学成分的影响.照明工程学报,27(2):112-117]
Chen Z(2016).Studies on the characterization of chlorogenic acids biosynthetic pathways and regulation of CGA content in Lonicera macranthoides Hand.-Mazz(dissertation).Chongqing:Chongqing University(in Chinese with English abstract)[陈泽雄(2016).灰毡毛忍冬(Lonicera macranthoides Hand.-Mazz)中绿原酸生物合成途径及调控技术研究(学位论文).重庆:重庆大学]
Dong H,Zhou Y,Tian Q,et al(2014).Effects of different light qualities on the contents of flavonoids of embryonic callus in Dimocarpus longan Lour.Chin J Trop Crops,35(12):2374-2377(in Chinese with English abstract)[董慧雪,周燕蓉,田奇琳等(2014).不同光质对龙眼胚性愈伤组织类黄酮含量的影响.热带作物学报,35(12):2374-2377]
Gao T,Si J,Zhu Y,et al(2012).Effects of light quality and germplasm on growth and effective ingredients of Dendrobium officinale germchit.China J Chin Mater Med,37(2):198-201(in Chinese with English abstract)[高亭亭,斯金平,朱玉球等(2012).光质与种质对铁皮石斛种苗生长和有效成分的影响.中国中药杂志,37(2):198-201]
Ge G,Li MY,Xue ZX,et al(2017).Effects of different light quality on the growth and metabolites accumulation in Inonotus sanghuang.J Chin Med Mater,40(5):1026-1030(in Chinese with English abstract)[葛格,李美芽,薛章贤等(2017).不同光质对桑黄的生长及代谢产物累积影响.中药材,40(5):1026-1030]
He JJ,Chen XD,Lan J(2011).Effect of light on growth in Ganoderma lucidum and anti-oxidative enzyme activities.Chin Trad Herb Drugs,42(12):2529-2534(in Chinese with English abstract)[郝俊江,陈向东,兰进(2011).光质对灵芝生长及抗氧化酶系统的影响.中草药,42(12):2529-2534]
Hu XT,Lu SZ,Bai Y,et al(2018).Effects of different shading treatments on physiology,biochemistry and total flavonoids of Tetrastigma hemsleyanum Diels et Gilg in Zhejiang Province.Guihaia,http://kns.cnki.net/kcms/detail/45.1134.Q.20180801.1850.002.html(in Chinese with English abstract)[胡晓甜,刘守赞,白岩等(2018).遮阴对浙江三叶青生理生化及总黄酮影响研究.广西植物,http://kns.cnki.net/kcms/detail/45.1134.Q.20180801.1850.002.html]
Ke X,Li JY,Li XY,et al(2011).Effects of different light quality on growth and photosynthesis of tobacco(Nicotiana tabacum L.)leaves.Plant Physiol J,47(5):512-520(in Chinese with English abstract)[柯学,李军营,李向阳等(2011).不同光质对烟草叶片生长及光合作用的影响.植物生理学报,47(5):512-520]
Liao S,Cai W,Chen D,et al(2017).Anti-inflammatory and analgesic effects of the extracts of Tetrastigmatis Hemsleyanum’s aerial parts from Fujian in vivo.Chin J Mod Appl Pharm,34(3):319-324(in Chinese with English abstract)[廖淑彬,蔡韦炜,陈丹等(2017).闽产三叶青地上部分提取物体内抗炎镇痛作用研究.中国现代应用药学,34(3):319-324]
Lin K,Huang Z,Jin X,et al(2017).Progress of research in light regulation for plants.Chin J Trop Crops,38(6):1163-1170(in Chinese with English abstract)[林魁,黄枝,金心怡等(2017).植物生长光调控应用研究进展.热带作物学报,38(6):1163-1170]
Lin S,Zhong L,Wei K(2016).Apoptosis-inducing effect of ethylacetate extracts of Sanyeqing(Tetrastigma Hemsleyanum)on colorectal cancer cell HT29 subcutaneous transplanted tumor.Chin J Trad Med Sci Technol,23(5):542-545(in Chinese with English abstract)[林霜,钟良瑞,魏克民(2016).三叶青乙酸乙酯提取物对结肠癌HT29细胞皮下移植瘤的诱导凋亡作用.中国中医药科技,23(5):542-545]
Liu YY,Zhou YF,Li Q,et al(2015).Study on leaf transpiration and stomatal structure of medicinal plant Tetrastigma hemsleyanum.Chin Trad Herb Drugs,46(17):2610-2617(in Chinese with English abstract)[刘崟艳,周以飞,李清等(2015).三叶青的蒸腾作用与气孔结构研究.中草药,46(17):2610-2617]
Luo MJ,Xia PG,Qi ZH,et al(2014).Effect of light quality on growth,photosynthesis and effective components of Panax notoginseng.China J Chin Mater Med,39(4):610-613(in Chinese with English abstract)[罗美佳,夏鹏国,齐志鸿等(2014).光质对三七生长、光合特性及有效成分积累的影响.中国中药杂志,39(4):610-613]
Pan JQ,Tong XR,Guo BL(2016).Progress of effects of light on plant flavonoids.China J Chin Mater Med,41(21):3897-3903(in Chinese with English abstract)[潘俊倩,佟曦然,郭宝林(2016).光对植物黄酮类化合物的影响研究进展.中国中药杂志,41(21):3897-3903]
Wu CF,Li JY,Li XY,et al(2011).Effects of color film shading on plastid pigment content in tobacco leaf.Acta Tabac Sin,17(6):48-53(in Chinese with English abstract)[邬春芳,李军营,李向阳等(2011).不同颜色薄膜遮光对烟草生长期质体色素含量的影响.中国烟草学报,17(6):48-53]
Xie BD,Wang HT(2006).Effects of light spectrum and photoperiod on contents of flavonoid and terpene in leaves of Ginkgo biloba L.J Nanjing For Univ Nat Sci Edit,30(2):51-54(in Chinese with English abstract)[谢宝东,王华田(2006).光质和光照时间对银杏叶片黄酮、内酯含量的影响.南京林业大学学报,30(2):51-54]
Xu DQ(1997).Some problems in somatal limitation analysis of photosynthesis.Plant Physiol Commun,33(4):241-244(in Chinese)[许大全(1997).光合作用气孔限制分析中的一些问题.植物生理学通讯,33(4):241-244]
Xu DQ,Gao W,Ruan J(2015).Effects of light quality on plant growth and development.Plant Physiol J,51(8):1217-1234(in Chinese with English abstract)[许大全,高伟,阮军(2015).光质对植物生长发育的影响.植物生理学报,51(8):1217-1234]
Yan MM,Wang ML,Wang HB,et al(2014).Effects of light quality on photosynthetic pigment contents and photosynthetic characteristics of peanut seedling leaves.Chin J Appl Ecol,25(2):483-487(in Chinese with English abstract)[闫萌萌,王铭伦,王洪波等(2014).光质对花生幼苗叶片光合色素含量及光合特性的影响.应用生态学报,25(2):483-487]
Yang J,Sha J,Gao X,et al(2017).Immune regulation function of flavonoids and its mechanisms.Chin J Anim Nutr,29(12):4295-4300(in Chinese with English abstract)[杨杰,沙金丹,高翔等(2017).黄酮类化合物的免疫调节作用及机制.动物营养学报,29(12):4295-4300]
Ye ZP,Kang HJ,Tao YL,et al(2010).Some problems on photosynthetic parameters calculated by photosynthesis assistant.Plant Physiol Commun,46(1):67-70(in Chinese)[叶子飘,康华靖,陶月良等(2010).以“光合助手”计算光合参数的一些问题.植物生理学通讯,46(1):67-70]
Yu Y,Liu S,Li C,et al(2015).Effects of LED light quality on the photosynthetic properties and physiological indexes of‘Summer black’grape.J Fruit Sci,32(5):879-884(in Chinese with English abstract)[余阳,刘帅,李春霞等(2015).LED光质对‘夏黑’葡萄光合特性和生理指标的影响.果树学报,32(5):879-884]
Zhang RH,Xu K,Dong CX(2008).Effect of light quality on photosynthetic characteristics of ginger leaves.Sci Agr Sin,41(11):3722-3727(in Chinese with English abstract)[张瑞华,徐坤,董灿兴(2008).光质对生姜叶片光合特性的影响.中国农业科学,41(11):3722-3727]
Zhang SQ,Zhang HY,Huang JW,et al(2017).Inhibitory effect of down-regulation of notch1 on Tetrastigma Hemsleyani radix flavone on migration and invasion of esophageal cancer EC9706 cells.Chin J Exp Trad Med Formulae,23(5):162-167(in Chinese with English abstract)[张胜强,张洪艳,黄建伟等(2017).Notch1下调对三叶青黄酮抑制食管癌EC9706细胞迁移和侵袭的影响.中国实验方剂学杂志,23(5):162-167]
Zhang Z,Qu W,Li X(2009).Experimental Guidance for Plant Physiology.4th ed.Beijing:Higher Education Press(in Chinese)[张志良,瞿伟菁,李小方(2009).植物生理学实验指导.第4版.北京:高等教育出版社]
Zhao DX,Li MY,XinJ M,et al(1999).Effects of light on cell growth and flavonoids biosynthesis in callus cultures of Saussurea medusa Maxim.Acta Phytophysiol Sin,25(2):127-132(in Chinese with English abstract)[赵德修,李茂寅,邢建民等(1999).光质、光强和光期对水母雪莲愈伤组织生长和黄酮生物合成的影响.植物生理学报,25(2):127-132]
Zhong LR,Lin S,Wei KM(2016).Inhibitory effects of radix Tetrastigma Hemsleyani flavone on growth and invasion of lung carcinoma cells.Chin Pharm Bull,32(4):480-483(in Chinese with English abstract)[钟良瑞,林霜,魏克民(2016).三叶青黄酮抗肺癌作用研究.中国药理学通报,32(4):480-483]
Zhong LR,Wei KM(2014).Radix tetrastigma hemsleyani flavone suppresses human lung carcinoma A549 cell by regulating MAPKs pathway.Chin Pharm Bull,30(1):101-104(in Chinese with English abstract)[钟良瑞,魏克民(2014).三叶青黄酮对肺癌A549细胞生长抑制与MAPKs通路关系的研究.中国药理学通报,30(1):101-104]
Zhou CB,Zhang X,Liu BB,et al(2015).The effect of supplementary light quality on physiological characteristics of lettuce.Plant Physiol J,51(12):2255-2262(in Chinese with English abstract)[周成波,张旭,刘彬彬等(2015).补光光质对叶用莴苣光合特性的影响.植物生理学报,51(12):2255-2262]
Chen M,Wu L,Cao L,et al(2016).Effect of light quality on growth and main chemical composition of tissue culture in Anoectochilus Roxburghii.China Illum Eng J,27(2):112-117(in Chinese with English abstract)[陈美香,武礼宾,曹立等(2016).光质对金线莲组培苗生长和主要化学成分的影响.照明工程学报,27(2):112-117]
Chen Z(2016).Studies on the characterization of chlorogenic acids biosynthetic pathways and regulation of CGA content in Lonicera macranthoides Hand.-Mazz(dissertation).Chongqing:Chongqing University(in Chinese with English abstract)[陈泽雄(2016).灰毡毛忍冬(Lonicera macranthoides Hand.-Mazz)中绿原酸生物合成途径及调控技术研究(学位论文).重庆:重庆大学]
Dong H,Zhou Y,Tian Q,et al(2014).Effects of different light qualities on the contents of flavonoids of embryonic callus in Dimocarpus longan Lour.Chin J Trop Crops,35(12):2374-2377(in Chinese with English abstract)[董慧雪,周燕蓉,田奇琳等(2014).不同光质对龙眼胚性愈伤组织类黄酮含量的影响.热带作物学报,35(12):2374-2377]
Gao T,Si J,Zhu Y,et al(2012).Effects of light quality and germplasm on growth and effective ingredients of Dendrobium officinale germchit.China J Chin Mater Med,37(2):198-201(in Chinese with English abstract)[高亭亭,斯金平,朱玉球等(2012).光质与种质对铁皮石斛种苗生长和有效成分的影响.中国中药杂志,37(2):198-201]
Ge G,Li MY,Xue ZX,et al(2017).Effects of different light quality on the growth and metabolites accumulation in Inonotus sanghuang.J Chin Med Mater,40(5):1026-1030(in Chinese with English abstract)[葛格,李美芽,薛章贤等(2017).不同光质对桑黄的生长及代谢产物累积影响.中药材,40(5):1026-1030]
He JJ,Chen XD,Lan J(2011).Effect of light on growth in Ganoderma lucidum and anti-oxidative enzyme activities.Chin Trad Herb Drugs,42(12):2529-2534(in Chinese with English abstract)[郝俊江,陈向东,兰进(2011).光质对灵芝生长及抗氧化酶系统的影响.中草药,42(12):2529-2534]
Hu XT,Lu SZ,Bai Y,et al(2018).Effects of different shading treatments on physiology,biochemistry and total flavonoids of Tetrastigma hemsleyanum Diels et Gilg in Zhejiang Province.Guihaia,http://kns.cnki.net/kcms/detail/45.1134.Q.20180801.1850.002.html(in Chinese with English abstract)[胡晓甜,刘守赞,白岩等(2018).遮阴对浙江三叶青生理生化及总黄酮影响研究.广西植物,http://kns.cnki.net/kcms/detail/45.1134.Q.20180801.1850.002.html]
Ke X,Li JY,Li XY,et al(2011).Effects of different light quality on growth and photosynthesis of tobacco(Nicotiana tabacum L.)leaves.Plant Physiol J,47(5):512-520(in Chinese with English abstract)[柯学,李军营,李向阳等(2011).不同光质对烟草叶片生长及光合作用的影响.植物生理学报,47(5):512-520]
Liao S,Cai W,Chen D,et al(2017).Anti-inflammatory and analgesic effects of the extracts of Tetrastigmatis Hemsleyanum’s aerial parts from Fujian in vivo.Chin J Mod Appl Pharm,34(3):319-324(in Chinese with English abstract)[廖淑彬,蔡韦炜,陈丹等(2017).闽产三叶青地上部分提取物体内抗炎镇痛作用研究.中国现代应用药学,34(3):319-324]
Lin K,Huang Z,Jin X,et al(2017).Progress of research in light regulation for plants.Chin J Trop Crops,38(6):1163-1170(in Chinese with English abstract)[林魁,黄枝,金心怡等(2017).植物生长光调控应用研究进展.热带作物学报,38(6):1163-1170]
Lin S,Zhong L,Wei K(2016).Apoptosis-inducing effect of ethylacetate extracts of Sanyeqing(Tetrastigma Hemsleyanum)on colorectal cancer cell HT29 subcutaneous transplanted tumor.Chin J Trad Med Sci Technol,23(5):542-545(in Chinese with English abstract)[林霜,钟良瑞,魏克民(2016).三叶青乙酸乙酯提取物对结肠癌HT29细胞皮下移植瘤的诱导凋亡作用.中国中医药科技,23(5):542-545]
Liu YY,Zhou YF,Li Q,et al(2015).Study on leaf transpiration and stomatal structure of medicinal plant Tetrastigma hemsleyanum.Chin Trad Herb Drugs,46(17):2610-2617(in Chinese with English abstract)[刘崟艳,周以飞,李清等(2015).三叶青的蒸腾作用与气孔结构研究.中草药,46(17):2610-2617]
Luo MJ,Xia PG,Qi ZH,et al(2014).Effect of light quality on growth,photosynthesis and effective components of Panax notoginseng.China J Chin Mater Med,39(4):610-613(in Chinese with English abstract)[罗美佳,夏鹏国,齐志鸿等(2014).光质对三七生长、光合特性及有效成分积累的影响.中国中药杂志,39(4):610-613]
Pan JQ,Tong XR,Guo BL(2016).Progress of effects of light on plant flavonoids.China J Chin Mater Med,41(21):3897-3903(in Chinese with English abstract)[潘俊倩,佟曦然,郭宝林(2016).光对植物黄酮类化合物的影响研究进展.中国中药杂志,41(21):3897-3903]
Wu CF,Li JY,Li XY,et al(2011).Effects of color film shading on plastid pigment content in tobacco leaf.Acta Tabac Sin,17(6):48-53(in Chinese with English abstract)[邬春芳,李军营,李向阳等(2011).不同颜色薄膜遮光对烟草生长期质体色素含量的影响.中国烟草学报,17(6):48-53]
Xie BD,Wang HT(2006).Effects of light spectrum and photoperiod on contents of flavonoid and terpene in leaves of Ginkgo biloba L.J Nanjing For Univ Nat Sci Edit,30(2):51-54(in Chinese with English abstract)[谢宝东,王华田(2006).光质和光照时间对银杏叶片黄酮、内酯含量的影响.南京林业大学学报,30(2):51-54]
Xu DQ(1997).Some problems in somatal limitation analysis of photosynthesis.Plant Physiol Commun,33(4):241-244(in Chinese)[许大全(1997).光合作用气孔限制分析中的一些问题.植物生理学通讯,33(4):241-244]
Xu DQ,Gao W,Ruan J(2015).Effects of light quality on plant growth and development.Plant Physiol J,51(8):1217-1234(in Chinese with English abstract)[许大全,高伟,阮军(2015).光质对植物生长发育的影响.植物生理学报,51(8):1217-1234]
Yan MM,Wang ML,Wang HB,et al(2014).Effects of light quality on photosynthetic pigment contents and photosynthetic characteristics of peanut seedling leaves.Chin J Appl Ecol,25(2):483-487(in Chinese with English abstract)[闫萌萌,王铭伦,王洪波等(2014).光质对花生幼苗叶片光合色素含量及光合特性的影响.应用生态学报,25(2):483-487]
Yang J,Sha J,Gao X,et al(2017).Immune regulation function of flavonoids and its mechanisms.Chin J Anim Nutr,29(12):4295-4300(in Chinese with English abstract)[杨杰,沙金丹,高翔等(2017).黄酮类化合物的免疫调节作用及机制.动物营养学报,29(12):4295-4300]
Ye ZP,Kang HJ,Tao YL,et al(2010).Some problems on photosynthetic parameters calculated by photosynthesis assistant.Plant Physiol Commun,46(1):67-70(in Chinese)[叶子飘,康华靖,陶月良等(2010).以“光合助手”计算光合参数的一些问题.植物生理学通讯,46(1):67-70]
Yu Y,Liu S,Li C,et al(2015).Effects of LED light quality on the photosynthetic properties and physiological indexes of‘Summer black’grape.J Fruit Sci,32(5):879-884(in Chinese with English abstract)[余阳,刘帅,李春霞等(2015).LED光质对‘夏黑’葡萄光合特性和生理指标的影响.果树学报,32(5):879-884]
Zhang RH,Xu K,Dong CX(2008).Effect of light quality on photosynthetic characteristics of ginger leaves.Sci Agr Sin,41(11):3722-3727(in Chinese with English abstract)[张瑞华,徐坤,董灿兴(2008).光质对生姜叶片光合特性的影响.中国农业科学,41(11):3722-3727]
Zhang SQ,Zhang HY,Huang JW,et al(2017).Inhibitory effect of down-regulation of notch1 on Tetrastigma Hemsleyani radix flavone on migration and invasion of esophageal cancer EC9706 cells.Chin J Exp Trad Med Formulae,23(5):162-167(in Chinese with English abstract)[张胜强,张洪艳,黄建伟等(2017).Notch1下调对三叶青黄酮抑制食管癌EC9706细胞迁移和侵袭的影响.中国实验方剂学杂志,23(5):162-167]
Zhang Z,Qu W,Li X(2009).Experimental Guidance for Plant Physiology.4th ed.Beijing:Higher Education Press(in Chinese)[张志良,瞿伟菁,李小方(2009).植物生理学实验指导.第4版.北京:高等教育出版社]
Zhao DX,Li MY,XinJ M,et al(1999).Effects of light on cell growth and flavonoids biosynthesis in callus cultures of Saussurea medusa Maxim.Acta Phytophysiol Sin,25(2):127-132(in Chinese with English abstract)[赵德修,李茂寅,邢建民等(1999).光质、光强和光期对水母雪莲愈伤组织生长和黄酮生物合成的影响.植物生理学报,25(2):127-132]
Zhong LR,Lin S,Wei KM(2016).Inhibitory effects of radix Tetrastigma Hemsleyani flavone on growth and invasion of lung carcinoma cells.Chin Pharm Bull,32(4):480-483(in Chinese with English abstract)[钟良瑞,林霜,魏克民(2016).三叶青黄酮抗肺癌作用研究.中国药理学通报,32(4):480-483]
Zhong LR,Wei KM(2014).Radix tetrastigma hemsleyani flavone suppresses human lung carcinoma A549 cell by regulating MAPKs pathway.Chin Pharm Bull,30(1):101-104(in Chinese with English abstract)[钟良瑞,魏克民(2014).三叶青黄酮对肺癌A549细胞生长抑制与MAPKs通路关系的研究.中国药理学通报,30(1):101-104]
Zhou CB,Zhang X,Liu BB,et al(2015).The effect of supplementary light quality on physiological characteristics of lettuce.Plant Physiol J,51(12):2255-2262(in Chinese with English abstract)[周成波,张旭,刘彬彬等(2015).补光光质对叶用莴苣光合特性的影响.植物生理学报,51(12):2255-2262]