基于16S rDNA高通量测序的玉米根际与非根际土壤细菌多样性比较
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摘要
以玉米根际土壤(RS)与非根际土壤(NRS)为研究对象,测定了土壤养分含量;并比较了土壤细菌多样性。结果显示,与RS相比,NRS中裂解氮(AN)、有效磷(AP)和有机碳(SOC)的含量以及碳氮比(C/N)均较高;在门的水平,有3个门的相对丰度较低,4个门的相对丰度则较高;在属水平,37个属的相对丰度较低,7个属的相对丰度较高。冗余分析结果表明,Bacillus、Chitinophaga、Pseudomonas、Lysobacter、Nocardioides、Balneimonas、Saccharothrix和Streptomyces的相对丰度与AN和SOC的含量及C/N呈正相关;Gemmata和Planctomyces的丰度与AP含量呈正相关;Bradyrhizobium的丰度与AK含量呈正相关。
Taking soil rhizosphere soil(RS)and non-rhizosphere soil(NRS)as the research objects,this paper determined the content of soil nutrients and compared the soil bacterial diversity. The results showed that compared with RS, NRS had higher content of available nitrogen(AN), available phosphorus(AP), organic carbon(SOC)and the carbon to nitrogen ratio(C/N). At the phylum level,the relative abundance of three phyla was lower and four phyla was higher in NRS than in RS. At the genus level, the relative abundance of 37 genera was lower and 7 genera was higher in NRS than in RS. The redundancy analysis showed that the relative abundances of Bacillus, Chitinophaga, Pseudomonas, Lysobacter, Nocardioides, Balneimonas, Saccharothrix and Streptomyces were positively correlated with AN, SOC and C/N. The abundance of Gemmata and Planctomyces was positively correlated with AP. The abundance of Bradyrhizobium was positively correlated with AK.
Taking soil rhizosphere soil(RS)and non-rhizosphere soil(NRS)as the research objects,this paper determined the content of soil nutrients and compared the soil bacterial diversity. The results showed that compared with RS, NRS had higher content of available nitrogen(AN), available phosphorus(AP), organic carbon(SOC)and the carbon to nitrogen ratio(C/N). At the phylum level,the relative abundance of three phyla was lower and four phyla was higher in NRS than in RS. At the genus level, the relative abundance of 37 genera was lower and 7 genera was higher in NRS than in RS. The redundancy analysis showed that the relative abundances of Bacillus, Chitinophaga, Pseudomonas, Lysobacter, Nocardioides, Balneimonas, Saccharothrix and Streptomyces were positively correlated with AN, SOC and C/N. The abundance of Gemmata and Planctomyces was positively correlated with AP. The abundance of Bradyrhizobium was positively correlated with AK.
引文
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[11]FAGEN J R,GIONGO A,BROWN C T,et al.Characterization of the relative abundance of the citrus pathogen Ca.liberibacter asiaticus in the microbiome of its insectvecor,Diaphorinacitri,using high throughput 16S r RNA sequencing[J].Open Microbiology Journal,2012,6(1):29-33.
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[17]RAMIREZ K S,CRAINE J M,FIERER N.Consistent effects of nitrogen amendments on soil microbial communities and processes across biomes[J].Global Change Biology,2012,18:1918-1927.
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[22]WIERINGA K T.The humification of high-moor peat[J].Plant and Soil,1964,21(3):333-344.
[23]JARIYAL M,GUPTA V K.Isolation and evaluation of potent Pseudomonas species for bioremediation of phorate in amended soil[J].Ecotoxicology and Environmental Safety,2015,122:24-30.
[24]NEDASHKOVSKAYA O I,BALABANOVA L A,ZHUKOVA NV,et al.Flavobacterium ahnfeltiae sp.nov.,a new marine polysaccharide-degrading bacterium isolated from a Pacific red alga[J].Archives of Microbiology,2014,196(10):745-752.
[25]LARSBRINK J.Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis[J].Journal of Proteomics,2017,156:63-74.
[26]SUN L N,ZHANG J,GONG F F,et al.Nocardioides soli sp.nov.a carbendazim-degrading bacterium isolated from soil under the long-term application of carbendazim[J].International Journal of Systematic and Evolutionary Microbiology,2014,64(Pt 6):2047.
[27]HAYWARD A C,FEGAN N,FEGAN M,et al.Stenotrophomonas and Lysobacter:ubiquitous plant-associated gamma-proteobacteria of developing significance in applied microbiology[J].Journal of Applied Microbiology,2010,108:756-770.
[28]MITHFER A,BHAGWAT A A,FEGER M,et al.Suppression of fungalβ-glucan-induced plant defence in soybean(Glycine max L.)by cyclic 1,3-1,6-β-glucans from the symbiont Bradyrhizobium japonicum[J].Planta,1996,199:270-275.
[29]OMAR S A,ABD-ALLA M H.Biocontrol of fungal root rot diseases of crop plants by the use of Rhizobia and Bradyrhizobia[J].Folia Microbiologica,1998,43:431-437.
[30]ZHANG X X,ZHANG R J,GAO J S,et al.Thirty-one years of rice-rice green manure rotations shape the rhizosphere microbial community and enrich beneficial bacteria[J].Soil Biology and Biochemistry,2017,104:208-217.
[2]董艳辉,于宇凤,温鑫,等.基于高通量测序的藜麦连作根际土壤微生物多样性研究[J].华北农学报,2019,34(2):205-211.
[3]胡静,侯向阳,王珍,等.割草和放牧对大针茅根际与非根际土壤养分和微生物数量的影响[J].应用生态学报,2016,26(11):3482-3488.
[4]丁新景,敬如岩,黄雅丽,等.黄河三角洲刺槐根际与非根际细菌结构及多样性[J].土壤学报,2017(5):1293-1302.
[5]程扬,刘子丹,沈启斌,等.秸秆生物炭施用对玉米根际和非根际土壤微生物群落结构的影响[J].生态环境学报,2018,27(10):92-99.
[6]LI X Z,RUI J P,MAO Y J,et al.Dynamics of the bacterial community structure in the rhizosphere of a maize cultivar[J].Soil Biology and Biochemistry,2014,68:392-401.
[7]姜涛.氮肥运筹对夏玉米产量、品质及植株养分含量的影响[J].植物营养与肥料学报,2013,19(3):559-565.
[8]刘卫玲,程思贤,周金龙,等.深松(耕)时机与方式对土壤物理性状和玉米产量的影响[J].河南农业科学,2018,47(3):13-19.
[9]张治家,张红,杜慧平.玉米品种对山西各地区大斑病生理小种抗性初探[J].天津农业科学,2013,19(6):56-60.
[10]鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,2000.
[11]FAGEN J R,GIONGO A,BROWN C T,et al.Characterization of the relative abundance of the citrus pathogen Ca.liberibacter asiaticus in the microbiome of its insectvecor,Diaphorinacitri,using high throughput 16S r RNA sequencing[J].Open Microbiology Journal,2012,6(1):29-33.
[12]CAPORASO G J,KUCZYNSKI J,STOMBAUGH J,et al.QIIMEallows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7:335-336.
[13]滑小赞,程滨,赵瑞芬.太原市农田土壤养分和重金属累积特征及相关性分析[J].山西农业科学,2019,47(6):1027-1033.
[14]XU L X,HAN Y S,YI M,et al.Shift of millet rhizosphere bacterial community during the maturation of parent soil revealed by 16Sr DNA high-throughput sequencing[J].Applied Soil Ecology,2019,13:157-165.
[15]刘晓伟,谢丹平,李开明,等.溶解氧变化对底泥酶活性及微生物多样性的影响[J].环境科学与技术,2013,36(6):6-11.
[16]FIERER N,BRADFORD M A.Toward an ecological classification of soil bacteria[J].Ecology,2007,88(6):1354-1364.
[17]RAMIREZ K S,CRAINE J M,FIERER N.Consistent effects of nitrogen amendments on soil microbial communities and processes across biomes[J].Global Change Biology,2012,18:1918-1927.
[18]OWARNAH H I,DAHUNSI S O,ORANUSI U S,et al.Fertilizer and sanitary quality of digestate biofertilizer from the co-digestion of food waste and human excreta[J].Waste Management,2014,34(4):747-752.
[19]DAHAL B,NANDAKAFLE G,PERKINS L,et al.Diversity of free-Living nitrogen fixing Streptomyces in soils of the badlands of South Dakota[J].Microbiol.Res.,2017,195:31-39.
[20]MANULIS S,SHAFRIR H,EPSTEIN E,et al.Biosynthesis of indole-3-acetic acid via the indole-3-acetamide pathway in Streptomyces spp.[J].Microbiol.,1994,140:1045.
[21]ARDLEY J K,PARKER MA,D E MEYER S E,et al.Microvirga lupini sp.nov.:Microvirga lotononidis sp.nov.and Microvirga zambiensis sp.nov.are alphaproteobacterial root-nodule bacteria that specifically nodulate and fix nitrogen with geographically and taxonomically separate legume hosts[J].International Journal of Systematic and Evolutionary Microbiology,2012,62:2579-2588.
[22]WIERINGA K T.The humification of high-moor peat[J].Plant and Soil,1964,21(3):333-344.
[23]JARIYAL M,GUPTA V K.Isolation and evaluation of potent Pseudomonas species for bioremediation of phorate in amended soil[J].Ecotoxicology and Environmental Safety,2015,122:24-30.
[24]NEDASHKOVSKAYA O I,BALABANOVA L A,ZHUKOVA NV,et al.Flavobacterium ahnfeltiae sp.nov.,a new marine polysaccharide-degrading bacterium isolated from a Pacific red alga[J].Archives of Microbiology,2014,196(10):745-752.
[25]LARSBRINK J.Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis[J].Journal of Proteomics,2017,156:63-74.
[26]SUN L N,ZHANG J,GONG F F,et al.Nocardioides soli sp.nov.a carbendazim-degrading bacterium isolated from soil under the long-term application of carbendazim[J].International Journal of Systematic and Evolutionary Microbiology,2014,64(Pt 6):2047.
[27]HAYWARD A C,FEGAN N,FEGAN M,et al.Stenotrophomonas and Lysobacter:ubiquitous plant-associated gamma-proteobacteria of developing significance in applied microbiology[J].Journal of Applied Microbiology,2010,108:756-770.
[28]MITHFER A,BHAGWAT A A,FEGER M,et al.Suppression of fungalβ-glucan-induced plant defence in soybean(Glycine max L.)by cyclic 1,3-1,6-β-glucans from the symbiont Bradyrhizobium japonicum[J].Planta,1996,199:270-275.
[29]OMAR S A,ABD-ALLA M H.Biocontrol of fungal root rot diseases of crop plants by the use of Rhizobia and Bradyrhizobia[J].Folia Microbiologica,1998,43:431-437.
[30]ZHANG X X,ZHANG R J,GAO J S,et al.Thirty-one years of rice-rice green manure rotations shape the rhizosphere microbial community and enrich beneficial bacteria[J].Soil Biology and Biochemistry,2017,104:208-217.
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