大气颗粒物中微生物分析方法及分布特征的研究进展
|
摘要
大气颗粒物(particulate matter, PM)可能携带病原微生物,会对人体健康产生影响,因此,对大气颗粒物中微生物的采集和分析已成为当今的研究热点。本文对大气颗粒物中微生物的采集方法和分析方法(培养法、菌群多样性组成谱测序和宏基因组学技术)的研究进展进行简要概括,着重介绍不同条件下(区域、时间、粒径、天气条件和气象因素)大气颗粒物中微生物的分布状况差异,并对引起相关差异的原因进行了探讨,旨在为深入认识大气颗粒物中微生物的分布特征提供参考。
Accumulating evidence suggests that particulate matter could carry pathogenic microorganisms which had adverse effects on human health, and thus current studies have been focusing on the collection and analysis of pathogenic microorganisms in particulate matter. The collection and analysis methods(culture method, microbial diversity compositional spectrum sequencing, and metagenomics technology) of microorganisms in particulate matter were summarized briefly in this study. Furthermore, the different distribution characteristics of microorganisms in particulate matter in different conditions(spatial, time, particle size, weather conditions, and meteorological factors) were reviewed. The causes of the differences among these distribution characteristics were then analyzed to provide a reference for further understanding of the distribution characteristics of microorganisms in particulate matter.
Accumulating evidence suggests that particulate matter could carry pathogenic microorganisms which had adverse effects on human health, and thus current studies have been focusing on the collection and analysis of pathogenic microorganisms in particulate matter. The collection and analysis methods(culture method, microbial diversity compositional spectrum sequencing, and metagenomics technology) of microorganisms in particulate matter were summarized briefly in this study. Furthermore, the different distribution characteristics of microorganisms in particulate matter in different conditions(spatial, time, particle size, weather conditions, and meteorological factors) were reviewed. The causes of the differences among these distribution characteristics were then analyzed to provide a reference for further understanding of the distribution characteristics of microorganisms in particulate matter.
引文
[1]Walser S M,Gerstner D G,Brenner B,et al.Evaluation of exposure-response relationships for health effects of microbial bioaerosols-A systematic review[J].International Journal of Hygiene and Environmental Health,2015,218:577-589
[2]Gandolfi I,Bertolini V,Ambrosini R,et al.Unravelling the bacterial diversity in the atmosphere[J].Applied Microbiology and Biotechnology,2013,97(11):4727-4736
[3]Singh D K,Gupta T.Role of transition metals with water soluble organic carbon in the formation of secondary organic aerosol and metallo-organics in PM1sampled during post monsoon and pre-winter time[J].Journal of Aerosol Science,2016,94:56-69
[4]Liu H,Zhang X,Zhang H,et al.Effect of air pollution on the total bacteria and pathogenic bacteria in different sizes of particulate matter[J].Environmental Pollution,2018,233:483-493
[5]薛银刚,刘菲,王利平,等.气溶胶中抗生素抗性基因研究进展:以养殖场和医院为例[J].生态毒理学报,2017,12(6):27-37Xue Y G,Liu F,Wang L P,et al.Progress in research on antibiotic resistance genes in aerosols:Take farms and hospitals as examples[J].Asian Journal of Ecotoxicology,2017,12(6):27-37(in Chinese)
[6]Yoo K,Lee T K,Choi E J,et al.Molecular approaches for the detection and monitoring of microbial communities in bioaerosols:A review[J].Journal of Environmental Sciences,2017,51(1):234-247
[7]Mbareche H,Brisebois E,Veillette M,et al.Bioaerosol sampling and detection methods based on molecular approaches:No pain no gain[J].Science of the Total Environment,2017,599:2095-2104
[8]廖旭.厦门市城区空气中的微生物组成及分布特征[D].厦门:集美大学,2013:18-19Liao X.Study on the community composition and distribution characteristics of microorganisms in the air of Xiamen City[D].Xiamen:Jimei University,2013:18-19(in Chinese)
[9]Haas D,Galler H,Luxner J,et al.The concentrations of culturable microorganisms in relation to particulate matter in urban air[J].Atmospheric Environment,2013,65(2):215-222
[10]Tu Q C,Yu H,He Z L,et al.Geo Chip 4:A functional gene-array-based high-throughput environmental technology for microbial community analysis[J].Molecular E-cology Resources,2014,14(5):914-928
[11]Qi J H,Zhong X,Shao Q.Microbial activity levels in atmospheric bioaerosols in Qingdao[J].Aerobiologia,2015,31(3):353-365
[12]Zhong X,Qi J H,Li H T,et al.Seasonal distribution of microbial activity in bioaerosols in the outdoor environment of the Qingdao coastal region[J].Atmospheric Environment,2016,140:506-513
[13]Hara K,Zhang D Z.Bacterial abundance and viability in long-range transported dust[J].Atmospheric Environment,2012,47(47):20-25
[14]房文艳,高新磊,李继,等.城市社区农贸市场空气微生物及抗生素抗性基因研究[J].生态毒理学报,2015,10(5):95-99Fang W Y,Gao X L,Li J,et al.Investigation on airborne bacteria and antibiotic resistance genes in urban community farmers market[J].Asian Journal of Ecotoxicology,2015,10(5):95-99(in Chinese)
[15]Lee S H,Lee H J,Kim S J,et al.Identification of airborne bacterial and fungal community structures in an urban area by T-RFLP analysis and quantitative real-time PCR[J].Science of Total Environment,2010,408(6):1349-1357
[16]宋森.兰州不同功能区及重点污染区大气微生物气溶胶分布特征研究[D].兰州:兰州交通大学,2017:31-77Song S.Study on distribution characteristics of atmospheric microbial aerosols in different functional areas and key polluted areas in Lanzhou City[D].Lanzhou:Lanzhou Jiaotong University,2017:31-77(in Chinese)
[17]Wang W F,Ma Y T,Ma X,et al.Seasonal variations of airborne bacteria in the Mogao Grottoes,Dunhuang,China[J].International Biodeterioration and Biodegradation,2010,64(4):309-315
[18]Qi J H,Shao Q,Xu W B,et al.Seasonal distribution of bioaerosols in the coastal region of Qingdao[J].Journal of Ocean University of China,2014,13(1):57-65
[19]王琳.青岛市市区街道和人工湿地空气微生物群落结构研究[D].青岛:青岛理工大学,2014:36-89Wang L.Studies on air microbial community structure at urban streets and wetland in Qingdao[D].Qingdao:Qingdao Technological University,2014:36-89(in Chinese)
[20]李彦鹏,刘鹏霞,谢铮胜,等.霾污染天气大气微生物气溶胶特性的研究进展[J].科学通报,2018,63(10):940-953Li Y P,Liu P X,Xie Z S,et al.Advances in research on atmospheric microbial aerosol characteristics in hazy days[J].Chinese Science Bulletin,2018,63(10):940-953(in Chinese)
[21]Li M F,Qi J H,Zhang H D,et al.Concentration and size distribution of bioaerosols in an outdoor environment in the Qingdao coastal region[J].Science of Total Environment,2011,409(19):3812-3819
[22]Jeon E M,Kim H J,Jung K,et al.Impact of Asian dust events on airborne bacterial community assessed by molecular analyses[J].Atmospheric Environment,2011,45(25):4313-4321
[23]Fahlgren C,Bratbak G,Sandaa R A,et al.Diversity of airborne bacteria in samples collected using different devices for aerosol collection[J].Aerobiologia,2011,27(2):107-120
[24]Griffin D W.Atmospheric movement of microorganisms in clouds of desert dust and implications for human health[J].Clinical Microbiology Reviews,2007,20(3):459-477
[25]Agranovski I E,Safatov A S,Borodulin A I,et al.Inactivation of viruses in bubbling processes utilized for personal bioaerosol monitoring[J].Applied and Environmental Microbiology,2004,70(12):6963-6967
[26]王彦杰,李琳,许光素,等.微生物气溶胶采集技术的特点及应用[J].微生物学通报,2017,44(3):701-709Wang Y J,Li L,Xu G S,et al.The application and characteristics of technologies for bioaerosols collection[J].Microbiology China,2017,44(3):701-709(in Chinese)
[27]Cao C,Jiang W,Wang B,et al.Inhalable microorganisms in Beijings PM2.5and PM10pollutants during a severe smog event[J].Environment Science and Technology,2014,48(3):1499-1507
[28]Xu C H,Wei M,Chen J M,et al.Bacterial characterization in ambient submicron particles during severe haze episodes at Ji’nan,China[J].Science of the Total Environment,2017,580:188-196
[29]苟欢歌,谢纯斌,童延斌,等.石河子市春季TSP和PM10中微生物群落特征[J].环境工程学报,2017,11(4):2343-2349Gou H G,Xie C B,Tong Y B,et al.Assessment of microbial communities in TSP and PM10of Shihezi during spring[J].Chinese Journal of Environmental Engineering,2017,11(4):2343-2349(in Chinese)
[30]Gou H G,Lu J J,Li S M,et al.Assessment of microbial communities in PM1and PM10of Urumqi during winter[J].Environmental Pollution,2016,214:202-210
[31]曹军骥.PM2.5与环境[M].北京:科学出版社,2014:20
[32]贺克斌.大气颗粒物与区域复合污染[M].北京:科学出版社,2011:58
[33]Chow J C.Measurement methods to determine compliance with ambient air quality standards for suspended particles[J].Journal of the Air and Waste Management Association,1995,45(5):320-382
[34]Awad A H,Gibbs S G,Tarwater P M,et al.Coarse and fine culturable fungal air concentrations in urban and rural homes in Egypt[J].International Journal of Environmental Research and Public Health,2013,10(3):936-949
[35]Chang C W,Hung N T,Chen N T.Optimization and application of propidium monoazide quantitative PCR method for viable bacterial bioaerosols[J].Journal of Aerosol Science,2017,104:90-99
[36]Emerson J B,Keady P B,Brewer T E,et al.Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood[J].Environment Science and Technology,2015,49(5):2675-2684
[37]孙欣,高莹,杨云锋.环境微生物的宏基因组学研究新进展[J].生物多样性,2013,21(4):393-400Sun X,Gao Y,Yang Y F.Recent advancement in microbial environmental research using metagenomics tools[J].Biodiversity Science,2013,21(4):393-400(in Chinese)
[38]Ravi A,Avershina E,Angell I L,et al.Comparison of reduced metagenome and 16S r RNA gene sequencing for determination of genetic diversity and mother-child overlap of the gut associated microbiota[J].Journal of Microbiological Methods,2018,149:44-52
[39]Lee T,Grinshpun S A,Kim K Y,et al.Relationship between indoor and outdoor bioaerosols collected with a button inhalable aerosol sampler in urban homes[J].Indoor Air,2006,16(1):37-47
[40]Xie Y Y,Fajardo O A,Yan W Z,et al.Six-day measurement of size-resolved indoor fluorescent bioaerosols of outdoor origin in an office[J].Particuology,2017,31(2):161-169
[41]Gao M,Jia R,Qiu T,et al.Seasonal size distribution of airborne culturable bacteria and fungi and preliminary estimation of their deposition in human lungs during nonhaze and haze days[J].Atmospheric Environment,2015,118:203-210
[42]Shade A,Hogan C S,Klimowicz A K,et al.Culturing captures members of the soil rare biosphere[J].Environmental Microbiology,2012,14(9):2247-2252
[43]Duan J C,Yu Y,Li Y,et al.Comprehensive understanding of PM2.5on gene and micro RNA expression patterns in zebrafish(Danio rerio)model[J].Science of the Total Environment,2017,586:666-674
[44]Bertolini V,Gandolfi I,Ambrosini R,et al.Temporal variability and effect of environmental variables on airborne bacterial communities in an urban area of Northern Italy[J].Applied Microbiology and Biotechnology,2013,97(14):6561-6570
[45]刘驰,李家宝,芮俊鹏,等.16S r RNA基因在微生物生态学中的应用[J].生态学报,2015,35(9):2769-2788Liu C,Li J B,Rui J P,et al.The applications of the 16Sr RNA gene in microbial ecology:Current situation and problems[J].Acta Ecologica Sinica,2015,35(9):2769-2788(in Chinese)
[46]Mandal M,Banerjee P S,Garg R,et al.Genetic characterization and phylogenetic relationships based on 18S r RNAand ITS1 region of small form of canine Babesia spp.from India[J].Infection Genetics and Evolution,2014,27(3):325-331
[47]周均亮,赵瑞琳.真菌DNA条形码技术研究进展[J].微生物学通报,2013,40(8):1468-1477Zhou J L,Zhao R L.Advances on DNA barcoding in fungi[J].Microbiology China,2013,40(8):1468-1477(in Chinese)
[48]Rittenour W R,Park J H,Coxganser J M,et al.Comparison of DNA extraction methodologies used for assessing fungal diversity via ITS sequencing[J].Journal of Environmental Monitoring,2012,14(3):766-774
[49]孟学平,申欣,程汉良,等.双壳纲贝类18S r RNA基因序列变异及系统发生[J].生态学报,2011,31(5):1393-1403Meng X P,Shen X,Cheng H L.18S r RNA gene variation and phylogenetic analysis among 6 orders of Bivalvia class[J].Acta Ecologica Sinica,2011,31(5):1393-1403(in Chinese)
[50]蔡俊龙.中国西南地区猕猴感染内阿米巴的分子流行病学调查[D].上海:复旦大学,2012:5-6Cai J L.Molecular identification of Entamoeba spp.among macaques in Southwest China[D].Shanghai:Fudan University,2012:5-6(in Chinese)
[51]Degois J,Clerc F,Simon X,et al.First metagenomic survey of the microbial diversity in bioaerosols emitted in waste sorting plants[J].Annals of Work Exposures and Health,2017,61(9):1076-1086
[52]刘洋荧,王尚,厉舒祯,等.基于功能基因的微生物碳循环分子生态学研究进展[J].微生物学通报,2017,44(7):1676-1689Liu Y Y,Wang S,Li S Z,et al.Advances in molecular ecology on microbial functional genes of carbon cycle[J].Microbiology China,2017,44(7):1676-1689(in Chinese)
[53]García-Gudi1o I,Yllescas-Medrano E,Maida-Claros R,et al.Microbiological comparison of blood culture and amplification of 16S r DNA methods in combination with DGGE for detection of neonatal sepsis in blood samples[J].European Journal of Pediatrics,2017,155-165:1-9
[54]Yergeau E,Hogues H,Whyte L G,et al.The functional potential of high Arctic permafrost revealed by metagenomic sequencing,q PCR and microarray analyses[J].ISME,2010,4(9):1206-1214
[55]Johny T K,Bhat S G.PCR in metagenomics[J].Methods in Molecular Biology,2017,1620:249-265
[56]廖旭,胡安谊,杨晓永,等.厦门冬季PM2.5颗粒物中细菌和真核微型生物群落组成及其来源分析[J].生态环境学报,2013,22(8):1395-1400Liao X,Hu A Y,Yang X Y,et al.Community composition and environmental sources of bacteria and eukaryotic microorganisms in fine air particulate(PM2.5)of Xiamen in winter[J].Ecology and Environmental Sciences,2013,22(8):1395-1400(in Chinese)
[57]Hirsch P R,Mauchline T H,Clark I M.Culture-independent molecular techniques for soil microbial ecology[J].Soil Biology and Biochemistry,2010,42(6):878-887
[58]Cong J,Liu X D,Lu H,et al.Analyses of the influencing factors of soil microbial functional gene diversity in tropical rainforest based on Geo Chip 5.0[J].Genomics Data,2015,5:397-398
[59]Tringe S G,Hugenholtz P.A renaissance for the pioneering 16S r RNA gene[J].Current Opinion in Microbiology,2008,11(5):442-446
[60]Ravin N V,Mardanov A V,Skryabin K G.Metagenomics as a tool for the investigation of uncultured microorganisms[J].Russian Journal of Genetics,2015,51(5):431-439
[61]魏子艳,金德才,邓晔.环境微生物宏基因组学研究中的生物信息学方法[J].微生物学通报,2015,42(5):890-901Wei Z Y,Jin D C,Deng Y.Bioinformatics tools and applications in the study of environmental microbial metagenomics[J].Microbiology China,2015,42(5):890-901(in Chinese)
[62]Bertaux J,Gloger U,Schmid M,et al.Routine flourescence in situ hybridization in soil[J].Journal of Microbiological Methods,2007,69(3):451-460
[63]Wagner M,Horn M,Daim S H.Fluorescence in situ hybridization for the identification and characterization of prokaryotes[J].Current Opinion in Microbiology,2003,6(3):302-309
[64]冯娇娇.流式细胞术在植物上的应用[D].杨凌:西北农林科技大学,2017:11-16Feng J J.Applications of flow cytometry on plant research[D].Yangling:Northwest Sci-tech University of Agriculture and Forestry,2017:11-16(in Chinese)
[65]Fierer N,Liu Z Z,Mari R H,et al.Short-term temporal variability in airborne bacterial and fungal populations[J].Applied and Environmental Microbiology,2008,74(1):200-207
[66]陈铭夏,金龙山,孙振海,等.南京市生物气溶胶浓度垂直分布和日变化规律[J].中国环境科学,2001,21(2):97-100Chen M X,Jin L S,Sun Z H,et al.The vertical distribution and diurnal variation law of the bioaerosol concentration in Nanjing City[J].China Environmental Science,2001,21(2):97-100(in Chinese)
[67]Gandolfi I,Bertolini V,Bestetti G,et al.Spatio-temporal variability of airborne bacterial communities and their correlation with particulate matter chemical composition across two urban areas[J].Applied Microbiology and Biotechnology,2015,99(11):1-11
[68]Bowers R M,Clements N,Emerson J B,et al.Seasonal variability in bacterial and fungal diversity of the nearsurface atmosphere[J].Environmental Science and Technology,2013,47(21):12097-12106
[69]Zhang J,Huang S,Tian M,et al.Microbial aerosol in Beijing-Tianjin-Hebei region of Eastern China[J].International Journal of Environment and Pollution,2017,61(2):89-97
[70]Woo A C,Brar M S,Chan Y,et al.Temporal variation in airborne microbial populations and microbially-derived allergens in a tropical urban landscape[J].Atmospheric Environment,2013,74(2):291-300
[71]Bowers R M,Sullivan A P,Costello E K,et al.Sources of bacteria in outdoor air across cities in the midwestern U-nited States[J].Applied and Environmental Microbiology,2011,77(18):6350-6356
[72]Gao M,Yan X,Qiu T,et al.Variation of correlations between factors and culturable airborne bacteria and fungi[J].Atmospheric Environment,2016,128:10-19
[73]韩晨,谢绵测,祁建华,等.青岛市不同空气质量下可培养生物气溶胶分布特征及影响因素[J].环境科学研究,2016,29(9):1264-1271Han C,Xie M C,Qi J H,et al.Size distribution characteristics of culturable bioaerosols in relation to air quality levels in Qingdao[J].Research of Environmental Sciences,2016,29(9):1264-1271(in Chinese)
[74]Bauer H,Kasper-Giebl A,L9flund M,et al.The contribution of bacteria and fungal spores to the organic carbon content of cloud water,precipitation and aerosols[J].Atmospheric Research,2002,64(1-4):109-119
[75]Adhikari A,Reponen T,Grinshpun S A,et al.Correlation of ambient inhalable bioaerosols with particulate matter and ozone:A two-year study[J].Environment Pollution,2006,140(1):16-28
[76]Ho H M,Rao C Y,Hsu H H,et al.Characteristics and determinants of ambient fungal spores in Hualien,Taiwan[J].Atmospheric Environment,2005,39(32):5839-5850
[77]Zuraimi M S,Fang L,Tan T K,et al.Airborne fungi in low and high allergic prevalence child care centers[J].Atmospheric Environment,2009,43(15):2391-2400
[78]Raisi L,Aleksandropoulou V,Lazaridis M,et al.Size distribution of viable,cultivable,airborne microbe sand their relationship to particulate matter concentrations and meteorological conditions in a Mediterranean site[J].Aerobiologia,2013,29(2):233-248
[79]Gao M,Qiu T L,Jia R Z,et al.Concentration and size distribution of bioaerosols at non-haze and haze days in Beijing[J].Environmental Science and Pollution Research,2015,22(6):4359-4368
[80]李婉欣,路瑞,谢铮胜,等.西安市秋冬季不同空气质量下可培养微生物气溶胶浓度和粒径分布[J].环境科学,2017,11(38):4494-4500Li W X,Lu R,Xie Z S,et al.Concentration and size distribution characteristics of culturable bioaerosols at various air quality levels during fall and winter in Xian,China[J].Environmental Science,2017,11(38):4494-4500(in Chinese)
[81]黄琼.南昌市PM10和PM2.5中微生物群落的分布及其相关性分析[D].南昌:南昌大学,2015:66-69Huang Q.The distribution and correlation of microflora in PM2.5and PM10in Nanchang[D].Nanchang:Nanchang University,2015:66-69(in Chinese)
[82]Alghamdi M A,Shamy M,Redal M A,et al.Microorganisms associated particulate matter:A preliminary study[J].Science of Total Environment,2014,479-480:109-116
[83]Yan D,Zhang T,Su J,et al.Diversity and composition of airborne fungal community associated with particulate matters in Beijing during haze and non-haze days[J].Frontiers in Microbiology,2016,7:487
[84]Li Y P,Wang W,Guo X,et al.Assessment of airborne bacteria and fungi in various university indoor environments:A case study in Changan University.China[J].Environment Engineering Science,2015,32(4):273-283
[85]Han Y P,Li L,Liu J X,et al.Microbial structure and chemical components of aerosols caused by rotating brushes in a wastewater treatment plant[J].Environmental Science and Pollution Research International,2012,19(9):4097-4108
[86]Zhen Q,Deng Y,Wang Y,et al.Meteorological factors had more impact on airborne bacterial communities than air pollutants[J].Science of the Total Environment,2017,601-602:703-712
[87]Dong L J,Qi J H,Shao C C,et al.Concentration and size distribution of total airborne microbes in hazy and foggy weather[J].Science of the Total Environment,2016,541:1011-1018
[88]Xie Z S,Li Y P,Lu R,et al.Characteristics of total airborne microbes at various air quality levels[J].Journal of Aerosol Science,2018,116:57-65
[89]Hwang S H,Park J B.Comparison of culturable airborne bacteria and related environmental factors at underground subway stations between 2006 and 2013[J].Atmospheric Environment,2014,84(2):289-293
[90]Smets W,Moretti S,Denys S,et al.Airborne bacteria in the atmosphere:Presence,purpose,and potential[J].Atmospheric Environment,2016,139:214-221
[91]Amato F,Schaap M,Hugo A C,et al.Effect of rain events on the mobility of road dust load in two Dutch and Spanish roads[J].Atmospheric Environment,2012,62(15):352-358
[92]Liang L L,Engling G,Cheng Y,et al.Rapid detection and quantification of fungal spores in the urban atmosphere by flow cytometry[J].Aerosol Science,2013,66(4):179-186
[93]Lu R,Li Y,Li W,et al.Bacterial community structure in atmospheric particulate matters of different sizes during the haze days in Xi'an,China[J].Science of the Total Environment,2018,637-638:244-252
[94]Hwang G B,Jung J H,Jeong T G,et al.Effect of hybrid UV-thermal energy stimuli on inactivation of S.epidermidis and B.subtilis bacterial bioaerosols[J].Science of Total Environment,2010,408(23):5903-5909
[95]许芸松,刘伟健,赵永志,等.黄、渤海滨海带大气颗粒物时空分布与来源特征[J].生态毒理学报,2017,12(3):346-357Xu Y S,Liu W J,Zhao Y Z,et al.Spatial and temporal distribution of atmospheric particle matter and source characteristics in the Littoral Areas of Bohai Sea and Yellow Sea[J].Asian Journal of Ecotoxicology,2017,12(3):346-357(in Chinese)
[96]Yu X,Wang Z,Zhang M,et al.Ambient measurement of fluorescent aerosol particles with a WIBS in the Yangtze River Delta of China:Potential impacts of combustion-related aerosol particles[J].Atmospheric Chemistry and Physics,2016,16(17):11337-11348
[97]Wolf R,El-Haddad I,Slowik J G,et al.Contribution of bacteria-like particles to PM2.5aerosol in urban and rural environments[J].Atmospheric Environment,2017,160:97-106
[2]Gandolfi I,Bertolini V,Ambrosini R,et al.Unravelling the bacterial diversity in the atmosphere[J].Applied Microbiology and Biotechnology,2013,97(11):4727-4736
[3]Singh D K,Gupta T.Role of transition metals with water soluble organic carbon in the formation of secondary organic aerosol and metallo-organics in PM1sampled during post monsoon and pre-winter time[J].Journal of Aerosol Science,2016,94:56-69
[4]Liu H,Zhang X,Zhang H,et al.Effect of air pollution on the total bacteria and pathogenic bacteria in different sizes of particulate matter[J].Environmental Pollution,2018,233:483-493
[5]薛银刚,刘菲,王利平,等.气溶胶中抗生素抗性基因研究进展:以养殖场和医院为例[J].生态毒理学报,2017,12(6):27-37Xue Y G,Liu F,Wang L P,et al.Progress in research on antibiotic resistance genes in aerosols:Take farms and hospitals as examples[J].Asian Journal of Ecotoxicology,2017,12(6):27-37(in Chinese)
[6]Yoo K,Lee T K,Choi E J,et al.Molecular approaches for the detection and monitoring of microbial communities in bioaerosols:A review[J].Journal of Environmental Sciences,2017,51(1):234-247
[7]Mbareche H,Brisebois E,Veillette M,et al.Bioaerosol sampling and detection methods based on molecular approaches:No pain no gain[J].Science of the Total Environment,2017,599:2095-2104
[8]廖旭.厦门市城区空气中的微生物组成及分布特征[D].厦门:集美大学,2013:18-19Liao X.Study on the community composition and distribution characteristics of microorganisms in the air of Xiamen City[D].Xiamen:Jimei University,2013:18-19(in Chinese)
[9]Haas D,Galler H,Luxner J,et al.The concentrations of culturable microorganisms in relation to particulate matter in urban air[J].Atmospheric Environment,2013,65(2):215-222
[10]Tu Q C,Yu H,He Z L,et al.Geo Chip 4:A functional gene-array-based high-throughput environmental technology for microbial community analysis[J].Molecular E-cology Resources,2014,14(5):914-928
[11]Qi J H,Zhong X,Shao Q.Microbial activity levels in atmospheric bioaerosols in Qingdao[J].Aerobiologia,2015,31(3):353-365
[12]Zhong X,Qi J H,Li H T,et al.Seasonal distribution of microbial activity in bioaerosols in the outdoor environment of the Qingdao coastal region[J].Atmospheric Environment,2016,140:506-513
[13]Hara K,Zhang D Z.Bacterial abundance and viability in long-range transported dust[J].Atmospheric Environment,2012,47(47):20-25
[14]房文艳,高新磊,李继,等.城市社区农贸市场空气微生物及抗生素抗性基因研究[J].生态毒理学报,2015,10(5):95-99Fang W Y,Gao X L,Li J,et al.Investigation on airborne bacteria and antibiotic resistance genes in urban community farmers market[J].Asian Journal of Ecotoxicology,2015,10(5):95-99(in Chinese)
[15]Lee S H,Lee H J,Kim S J,et al.Identification of airborne bacterial and fungal community structures in an urban area by T-RFLP analysis and quantitative real-time PCR[J].Science of Total Environment,2010,408(6):1349-1357
[16]宋森.兰州不同功能区及重点污染区大气微生物气溶胶分布特征研究[D].兰州:兰州交通大学,2017:31-77Song S.Study on distribution characteristics of atmospheric microbial aerosols in different functional areas and key polluted areas in Lanzhou City[D].Lanzhou:Lanzhou Jiaotong University,2017:31-77(in Chinese)
[17]Wang W F,Ma Y T,Ma X,et al.Seasonal variations of airborne bacteria in the Mogao Grottoes,Dunhuang,China[J].International Biodeterioration and Biodegradation,2010,64(4):309-315
[18]Qi J H,Shao Q,Xu W B,et al.Seasonal distribution of bioaerosols in the coastal region of Qingdao[J].Journal of Ocean University of China,2014,13(1):57-65
[19]王琳.青岛市市区街道和人工湿地空气微生物群落结构研究[D].青岛:青岛理工大学,2014:36-89Wang L.Studies on air microbial community structure at urban streets and wetland in Qingdao[D].Qingdao:Qingdao Technological University,2014:36-89(in Chinese)
[20]李彦鹏,刘鹏霞,谢铮胜,等.霾污染天气大气微生物气溶胶特性的研究进展[J].科学通报,2018,63(10):940-953Li Y P,Liu P X,Xie Z S,et al.Advances in research on atmospheric microbial aerosol characteristics in hazy days[J].Chinese Science Bulletin,2018,63(10):940-953(in Chinese)
[21]Li M F,Qi J H,Zhang H D,et al.Concentration and size distribution of bioaerosols in an outdoor environment in the Qingdao coastal region[J].Science of Total Environment,2011,409(19):3812-3819
[22]Jeon E M,Kim H J,Jung K,et al.Impact of Asian dust events on airborne bacterial community assessed by molecular analyses[J].Atmospheric Environment,2011,45(25):4313-4321
[23]Fahlgren C,Bratbak G,Sandaa R A,et al.Diversity of airborne bacteria in samples collected using different devices for aerosol collection[J].Aerobiologia,2011,27(2):107-120
[24]Griffin D W.Atmospheric movement of microorganisms in clouds of desert dust and implications for human health[J].Clinical Microbiology Reviews,2007,20(3):459-477
[25]Agranovski I E,Safatov A S,Borodulin A I,et al.Inactivation of viruses in bubbling processes utilized for personal bioaerosol monitoring[J].Applied and Environmental Microbiology,2004,70(12):6963-6967
[26]王彦杰,李琳,许光素,等.微生物气溶胶采集技术的特点及应用[J].微生物学通报,2017,44(3):701-709Wang Y J,Li L,Xu G S,et al.The application and characteristics of technologies for bioaerosols collection[J].Microbiology China,2017,44(3):701-709(in Chinese)
[27]Cao C,Jiang W,Wang B,et al.Inhalable microorganisms in Beijings PM2.5and PM10pollutants during a severe smog event[J].Environment Science and Technology,2014,48(3):1499-1507
[28]Xu C H,Wei M,Chen J M,et al.Bacterial characterization in ambient submicron particles during severe haze episodes at Ji’nan,China[J].Science of the Total Environment,2017,580:188-196
[29]苟欢歌,谢纯斌,童延斌,等.石河子市春季TSP和PM10中微生物群落特征[J].环境工程学报,2017,11(4):2343-2349Gou H G,Xie C B,Tong Y B,et al.Assessment of microbial communities in TSP and PM10of Shihezi during spring[J].Chinese Journal of Environmental Engineering,2017,11(4):2343-2349(in Chinese)
[30]Gou H G,Lu J J,Li S M,et al.Assessment of microbial communities in PM1and PM10of Urumqi during winter[J].Environmental Pollution,2016,214:202-210
[31]曹军骥.PM2.5与环境[M].北京:科学出版社,2014:20
[32]贺克斌.大气颗粒物与区域复合污染[M].北京:科学出版社,2011:58
[33]Chow J C.Measurement methods to determine compliance with ambient air quality standards for suspended particles[J].Journal of the Air and Waste Management Association,1995,45(5):320-382
[34]Awad A H,Gibbs S G,Tarwater P M,et al.Coarse and fine culturable fungal air concentrations in urban and rural homes in Egypt[J].International Journal of Environmental Research and Public Health,2013,10(3):936-949
[35]Chang C W,Hung N T,Chen N T.Optimization and application of propidium monoazide quantitative PCR method for viable bacterial bioaerosols[J].Journal of Aerosol Science,2017,104:90-99
[36]Emerson J B,Keady P B,Brewer T E,et al.Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood[J].Environment Science and Technology,2015,49(5):2675-2684
[37]孙欣,高莹,杨云锋.环境微生物的宏基因组学研究新进展[J].生物多样性,2013,21(4):393-400Sun X,Gao Y,Yang Y F.Recent advancement in microbial environmental research using metagenomics tools[J].Biodiversity Science,2013,21(4):393-400(in Chinese)
[38]Ravi A,Avershina E,Angell I L,et al.Comparison of reduced metagenome and 16S r RNA gene sequencing for determination of genetic diversity and mother-child overlap of the gut associated microbiota[J].Journal of Microbiological Methods,2018,149:44-52
[39]Lee T,Grinshpun S A,Kim K Y,et al.Relationship between indoor and outdoor bioaerosols collected with a button inhalable aerosol sampler in urban homes[J].Indoor Air,2006,16(1):37-47
[40]Xie Y Y,Fajardo O A,Yan W Z,et al.Six-day measurement of size-resolved indoor fluorescent bioaerosols of outdoor origin in an office[J].Particuology,2017,31(2):161-169
[41]Gao M,Jia R,Qiu T,et al.Seasonal size distribution of airborne culturable bacteria and fungi and preliminary estimation of their deposition in human lungs during nonhaze and haze days[J].Atmospheric Environment,2015,118:203-210
[42]Shade A,Hogan C S,Klimowicz A K,et al.Culturing captures members of the soil rare biosphere[J].Environmental Microbiology,2012,14(9):2247-2252
[43]Duan J C,Yu Y,Li Y,et al.Comprehensive understanding of PM2.5on gene and micro RNA expression patterns in zebrafish(Danio rerio)model[J].Science of the Total Environment,2017,586:666-674
[44]Bertolini V,Gandolfi I,Ambrosini R,et al.Temporal variability and effect of environmental variables on airborne bacterial communities in an urban area of Northern Italy[J].Applied Microbiology and Biotechnology,2013,97(14):6561-6570
[45]刘驰,李家宝,芮俊鹏,等.16S r RNA基因在微生物生态学中的应用[J].生态学报,2015,35(9):2769-2788Liu C,Li J B,Rui J P,et al.The applications of the 16Sr RNA gene in microbial ecology:Current situation and problems[J].Acta Ecologica Sinica,2015,35(9):2769-2788(in Chinese)
[46]Mandal M,Banerjee P S,Garg R,et al.Genetic characterization and phylogenetic relationships based on 18S r RNAand ITS1 region of small form of canine Babesia spp.from India[J].Infection Genetics and Evolution,2014,27(3):325-331
[47]周均亮,赵瑞琳.真菌DNA条形码技术研究进展[J].微生物学通报,2013,40(8):1468-1477Zhou J L,Zhao R L.Advances on DNA barcoding in fungi[J].Microbiology China,2013,40(8):1468-1477(in Chinese)
[48]Rittenour W R,Park J H,Coxganser J M,et al.Comparison of DNA extraction methodologies used for assessing fungal diversity via ITS sequencing[J].Journal of Environmental Monitoring,2012,14(3):766-774
[49]孟学平,申欣,程汉良,等.双壳纲贝类18S r RNA基因序列变异及系统发生[J].生态学报,2011,31(5):1393-1403Meng X P,Shen X,Cheng H L.18S r RNA gene variation and phylogenetic analysis among 6 orders of Bivalvia class[J].Acta Ecologica Sinica,2011,31(5):1393-1403(in Chinese)
[50]蔡俊龙.中国西南地区猕猴感染内阿米巴的分子流行病学调查[D].上海:复旦大学,2012:5-6Cai J L.Molecular identification of Entamoeba spp.among macaques in Southwest China[D].Shanghai:Fudan University,2012:5-6(in Chinese)
[51]Degois J,Clerc F,Simon X,et al.First metagenomic survey of the microbial diversity in bioaerosols emitted in waste sorting plants[J].Annals of Work Exposures and Health,2017,61(9):1076-1086
[52]刘洋荧,王尚,厉舒祯,等.基于功能基因的微生物碳循环分子生态学研究进展[J].微生物学通报,2017,44(7):1676-1689Liu Y Y,Wang S,Li S Z,et al.Advances in molecular ecology on microbial functional genes of carbon cycle[J].Microbiology China,2017,44(7):1676-1689(in Chinese)
[53]García-Gudi1o I,Yllescas-Medrano E,Maida-Claros R,et al.Microbiological comparison of blood culture and amplification of 16S r DNA methods in combination with DGGE for detection of neonatal sepsis in blood samples[J].European Journal of Pediatrics,2017,155-165:1-9
[54]Yergeau E,Hogues H,Whyte L G,et al.The functional potential of high Arctic permafrost revealed by metagenomic sequencing,q PCR and microarray analyses[J].ISME,2010,4(9):1206-1214
[55]Johny T K,Bhat S G.PCR in metagenomics[J].Methods in Molecular Biology,2017,1620:249-265
[56]廖旭,胡安谊,杨晓永,等.厦门冬季PM2.5颗粒物中细菌和真核微型生物群落组成及其来源分析[J].生态环境学报,2013,22(8):1395-1400Liao X,Hu A Y,Yang X Y,et al.Community composition and environmental sources of bacteria and eukaryotic microorganisms in fine air particulate(PM2.5)of Xiamen in winter[J].Ecology and Environmental Sciences,2013,22(8):1395-1400(in Chinese)
[57]Hirsch P R,Mauchline T H,Clark I M.Culture-independent molecular techniques for soil microbial ecology[J].Soil Biology and Biochemistry,2010,42(6):878-887
[58]Cong J,Liu X D,Lu H,et al.Analyses of the influencing factors of soil microbial functional gene diversity in tropical rainforest based on Geo Chip 5.0[J].Genomics Data,2015,5:397-398
[59]Tringe S G,Hugenholtz P.A renaissance for the pioneering 16S r RNA gene[J].Current Opinion in Microbiology,2008,11(5):442-446
[60]Ravin N V,Mardanov A V,Skryabin K G.Metagenomics as a tool for the investigation of uncultured microorganisms[J].Russian Journal of Genetics,2015,51(5):431-439
[61]魏子艳,金德才,邓晔.环境微生物宏基因组学研究中的生物信息学方法[J].微生物学通报,2015,42(5):890-901Wei Z Y,Jin D C,Deng Y.Bioinformatics tools and applications in the study of environmental microbial metagenomics[J].Microbiology China,2015,42(5):890-901(in Chinese)
[62]Bertaux J,Gloger U,Schmid M,et al.Routine flourescence in situ hybridization in soil[J].Journal of Microbiological Methods,2007,69(3):451-460
[63]Wagner M,Horn M,Daim S H.Fluorescence in situ hybridization for the identification and characterization of prokaryotes[J].Current Opinion in Microbiology,2003,6(3):302-309
[64]冯娇娇.流式细胞术在植物上的应用[D].杨凌:西北农林科技大学,2017:11-16Feng J J.Applications of flow cytometry on plant research[D].Yangling:Northwest Sci-tech University of Agriculture and Forestry,2017:11-16(in Chinese)
[65]Fierer N,Liu Z Z,Mari R H,et al.Short-term temporal variability in airborne bacterial and fungal populations[J].Applied and Environmental Microbiology,2008,74(1):200-207
[66]陈铭夏,金龙山,孙振海,等.南京市生物气溶胶浓度垂直分布和日变化规律[J].中国环境科学,2001,21(2):97-100Chen M X,Jin L S,Sun Z H,et al.The vertical distribution and diurnal variation law of the bioaerosol concentration in Nanjing City[J].China Environmental Science,2001,21(2):97-100(in Chinese)
[67]Gandolfi I,Bertolini V,Bestetti G,et al.Spatio-temporal variability of airborne bacterial communities and their correlation with particulate matter chemical composition across two urban areas[J].Applied Microbiology and Biotechnology,2015,99(11):1-11
[68]Bowers R M,Clements N,Emerson J B,et al.Seasonal variability in bacterial and fungal diversity of the nearsurface atmosphere[J].Environmental Science and Technology,2013,47(21):12097-12106
[69]Zhang J,Huang S,Tian M,et al.Microbial aerosol in Beijing-Tianjin-Hebei region of Eastern China[J].International Journal of Environment and Pollution,2017,61(2):89-97
[70]Woo A C,Brar M S,Chan Y,et al.Temporal variation in airborne microbial populations and microbially-derived allergens in a tropical urban landscape[J].Atmospheric Environment,2013,74(2):291-300
[71]Bowers R M,Sullivan A P,Costello E K,et al.Sources of bacteria in outdoor air across cities in the midwestern U-nited States[J].Applied and Environmental Microbiology,2011,77(18):6350-6356
[72]Gao M,Yan X,Qiu T,et al.Variation of correlations between factors and culturable airborne bacteria and fungi[J].Atmospheric Environment,2016,128:10-19
[73]韩晨,谢绵测,祁建华,等.青岛市不同空气质量下可培养生物气溶胶分布特征及影响因素[J].环境科学研究,2016,29(9):1264-1271Han C,Xie M C,Qi J H,et al.Size distribution characteristics of culturable bioaerosols in relation to air quality levels in Qingdao[J].Research of Environmental Sciences,2016,29(9):1264-1271(in Chinese)
[74]Bauer H,Kasper-Giebl A,L9flund M,et al.The contribution of bacteria and fungal spores to the organic carbon content of cloud water,precipitation and aerosols[J].Atmospheric Research,2002,64(1-4):109-119
[75]Adhikari A,Reponen T,Grinshpun S A,et al.Correlation of ambient inhalable bioaerosols with particulate matter and ozone:A two-year study[J].Environment Pollution,2006,140(1):16-28
[76]Ho H M,Rao C Y,Hsu H H,et al.Characteristics and determinants of ambient fungal spores in Hualien,Taiwan[J].Atmospheric Environment,2005,39(32):5839-5850
[77]Zuraimi M S,Fang L,Tan T K,et al.Airborne fungi in low and high allergic prevalence child care centers[J].Atmospheric Environment,2009,43(15):2391-2400
[78]Raisi L,Aleksandropoulou V,Lazaridis M,et al.Size distribution of viable,cultivable,airborne microbe sand their relationship to particulate matter concentrations and meteorological conditions in a Mediterranean site[J].Aerobiologia,2013,29(2):233-248
[79]Gao M,Qiu T L,Jia R Z,et al.Concentration and size distribution of bioaerosols at non-haze and haze days in Beijing[J].Environmental Science and Pollution Research,2015,22(6):4359-4368
[80]李婉欣,路瑞,谢铮胜,等.西安市秋冬季不同空气质量下可培养微生物气溶胶浓度和粒径分布[J].环境科学,2017,11(38):4494-4500Li W X,Lu R,Xie Z S,et al.Concentration and size distribution characteristics of culturable bioaerosols at various air quality levels during fall and winter in Xian,China[J].Environmental Science,2017,11(38):4494-4500(in Chinese)
[81]黄琼.南昌市PM10和PM2.5中微生物群落的分布及其相关性分析[D].南昌:南昌大学,2015:66-69Huang Q.The distribution and correlation of microflora in PM2.5and PM10in Nanchang[D].Nanchang:Nanchang University,2015:66-69(in Chinese)
[82]Alghamdi M A,Shamy M,Redal M A,et al.Microorganisms associated particulate matter:A preliminary study[J].Science of Total Environment,2014,479-480:109-116
[83]Yan D,Zhang T,Su J,et al.Diversity and composition of airborne fungal community associated with particulate matters in Beijing during haze and non-haze days[J].Frontiers in Microbiology,2016,7:487
[84]Li Y P,Wang W,Guo X,et al.Assessment of airborne bacteria and fungi in various university indoor environments:A case study in Changan University.China[J].Environment Engineering Science,2015,32(4):273-283
[85]Han Y P,Li L,Liu J X,et al.Microbial structure and chemical components of aerosols caused by rotating brushes in a wastewater treatment plant[J].Environmental Science and Pollution Research International,2012,19(9):4097-4108
[86]Zhen Q,Deng Y,Wang Y,et al.Meteorological factors had more impact on airborne bacterial communities than air pollutants[J].Science of the Total Environment,2017,601-602:703-712
[87]Dong L J,Qi J H,Shao C C,et al.Concentration and size distribution of total airborne microbes in hazy and foggy weather[J].Science of the Total Environment,2016,541:1011-1018
[88]Xie Z S,Li Y P,Lu R,et al.Characteristics of total airborne microbes at various air quality levels[J].Journal of Aerosol Science,2018,116:57-65
[89]Hwang S H,Park J B.Comparison of culturable airborne bacteria and related environmental factors at underground subway stations between 2006 and 2013[J].Atmospheric Environment,2014,84(2):289-293
[90]Smets W,Moretti S,Denys S,et al.Airborne bacteria in the atmosphere:Presence,purpose,and potential[J].Atmospheric Environment,2016,139:214-221
[91]Amato F,Schaap M,Hugo A C,et al.Effect of rain events on the mobility of road dust load in two Dutch and Spanish roads[J].Atmospheric Environment,2012,62(15):352-358
[92]Liang L L,Engling G,Cheng Y,et al.Rapid detection and quantification of fungal spores in the urban atmosphere by flow cytometry[J].Aerosol Science,2013,66(4):179-186
[93]Lu R,Li Y,Li W,et al.Bacterial community structure in atmospheric particulate matters of different sizes during the haze days in Xi'an,China[J].Science of the Total Environment,2018,637-638:244-252
[94]Hwang G B,Jung J H,Jeong T G,et al.Effect of hybrid UV-thermal energy stimuli on inactivation of S.epidermidis and B.subtilis bacterial bioaerosols[J].Science of Total Environment,2010,408(23):5903-5909
[95]许芸松,刘伟健,赵永志,等.黄、渤海滨海带大气颗粒物时空分布与来源特征[J].生态毒理学报,2017,12(3):346-357Xu Y S,Liu W J,Zhao Y Z,et al.Spatial and temporal distribution of atmospheric particle matter and source characteristics in the Littoral Areas of Bohai Sea and Yellow Sea[J].Asian Journal of Ecotoxicology,2017,12(3):346-357(in Chinese)
[96]Yu X,Wang Z,Zhang M,et al.Ambient measurement of fluorescent aerosol particles with a WIBS in the Yangtze River Delta of China:Potential impacts of combustion-related aerosol particles[J].Atmospheric Chemistry and Physics,2016,16(17):11337-11348
[97]Wolf R,El-Haddad I,Slowik J G,et al.Contribution of bacteria-like particles to PM2.5aerosol in urban and rural environments[J].Atmospheric Environment,2017,160:97-106
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |