蟛蜞菊水提液对水华蓝藻——卵孢金孢藻的化感作用研究
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摘要
为探究陆生植物对水华蓝藻的化感作用,本文采用不同浓度的蟛蜞菊(Wedelia chinensis)水提液对卵孢金孢藻(Chrysosporum ovalisporum)进行了为期6 d的室内培养试验,结果发现:蟛蜞菊水提液对卵孢金孢藻的化感作用总体上呈"低促高抑";当蟛蜞菊水提液浓度大于6 g·L~(-1)时,将显著降低卵孢金孢藻光合作用有效量子效率(YⅡ)、最大量子产量(Fv/Fm)、叶绿素a(Chla)含量和藻细胞生物量(P<0.01);当浓度大于8 g·L~(-1)时,单位藻细胞内的超氧化物歧化酶(SOD)、谷胱甘肽-S-转移酶(GST)活性显著高于对照组(P<0.05),表明蓝藻细胞受到较强的胁迫作用;透射电镜观察结果表明:高浓度蟛蜞菊水提液会破坏细胞膜,导致藻细胞内的类囊体、羧基体、气囊等超微结构受损,并最终导致藻细胞溶解、藻丝断裂和死亡。此外,本文利用Logisitic模型研究了蟛蜞菊水提液对卵孢金孢藻的半效应浓度(EC50),其24 h、48 h、72 h和144 h的EC50分别为:3.78 g·L~(-1)、4.47 g·L~(-1)、4.72 g·L~(-1)和6.55 g·L~(-1)。本研究为中国湖泊蓝藻水华的防治增添了基础数据和新的技术思路。
In order to explore the allelopathic effects of terrestrial plants on cyanobacterial species that cause algal bloom, Chrysosporum ovalisporum was exposed to different concentrations of Wedelia chinensis water extracts during a 6-day incubation. The results showed that low concentration extracts of W. chinensis significantly improved the growth of C. ovalisporum, while the high concentrations extracts showed significantly negative effect on C. ovalisporum. The values of potential maximal photochemical efficiency of PSⅡ(Fv/Fm), effective photochemical quantum yield of PSⅡ(YⅡ) and chlorophyll-a(Chl-a) were significantly reduced by the extracts when its concentrations were greater than 6 g·L~(-1)(P<0.01). Compared with the control group, the superoxide dismutase(SOD) and gutathione-S-transferase(GST) activity of C. ovalisporum in high concentration W. chinensis extracts treatment group(8 g·L~(-1)) increased significantly(P<0.05), which indicated that C. ovalisporum survive extreme environmental stress.Transmission electron microscope(TEM) pictures showed that ultra-structures of C. ovalisporum cells, such as thylakoid, carboxyl, gas vesicles and cell membranes were destroyed by the extracts of W. chinensis, consequently, resulting in the dissolution, fracture of C. ovalisporum filaments. In addition, the median effect concentration(EC50) of W. chinensis water extracts in 24 h, 48 h, 72 h and 144 h were 3.78 g·L~(-1), 4.47 g·L~(-1), 4.72 g·L~(-1) and 6.55 g·L~(-1),respectively, estimated by using Logisitic growth model. Our study contributes to the existing knowledge and data of freshwater cyanobacterial bloom, and provides insights for its control and freshwater system restoration infected by cyanobacterial bloom.
In order to explore the allelopathic effects of terrestrial plants on cyanobacterial species that cause algal bloom, Chrysosporum ovalisporum was exposed to different concentrations of Wedelia chinensis water extracts during a 6-day incubation. The results showed that low concentration extracts of W. chinensis significantly improved the growth of C. ovalisporum, while the high concentrations extracts showed significantly negative effect on C. ovalisporum. The values of potential maximal photochemical efficiency of PSⅡ(Fv/Fm), effective photochemical quantum yield of PSⅡ(YⅡ) and chlorophyll-a(Chl-a) were significantly reduced by the extracts when its concentrations were greater than 6 g·L~(-1)(P<0.01). Compared with the control group, the superoxide dismutase(SOD) and gutathione-S-transferase(GST) activity of C. ovalisporum in high concentration W. chinensis extracts treatment group(8 g·L~(-1)) increased significantly(P<0.05), which indicated that C. ovalisporum survive extreme environmental stress.Transmission electron microscope(TEM) pictures showed that ultra-structures of C. ovalisporum cells, such as thylakoid, carboxyl, gas vesicles and cell membranes were destroyed by the extracts of W. chinensis, consequently, resulting in the dissolution, fracture of C. ovalisporum filaments. In addition, the median effect concentration(EC50) of W. chinensis water extracts in 24 h, 48 h, 72 h and 144 h were 3.78 g·L~(-1), 4.47 g·L~(-1), 4.72 g·L~(-1) and 6.55 g·L~(-1),respectively, estimated by using Logisitic growth model. Our study contributes to the existing knowledge and data of freshwater cyanobacterial bloom, and provides insights for its control and freshwater system restoration infected by cyanobacterial bloom.
引文
[1]秦伯强,杨柳燕,陈非洲,等.湖泊富营养化发生机制与控制技术及其应用[J].科学通报,2006,51(16):1857-1866
[2]王扬才,陆开宏.蓝藻水华的危害及治理动态[J].水产学杂志,2004,17(1):90-94Wang Y C,Lu K H.Harmful effects of cyanobacteria and its control development[J].Chinese Journal of Fisheries,2004,17(1):90-94(in Chinese)
[3]Paul V J.Global warming and cyanobacterial harmful algal blooms[J].Advances in Experimental Medicine&Biology,2008,619:239-257
[4]Kosten S,Huszar V L M,Bécares E,et al.Warmer climates boost cyanobacterial dominance in shallow lakes[J].Global Change Biology,2012,18(1):118-126
[5]孔繁翔.湖泊富营养化治理与蓝藻水华控制[J].江苏科技信息,2007(9):7-11
[6]陈识文,毛涛,袁科平,等.水华治理方法研究进展[J].长江大学学报:自科版,2014,11(35):69-73
[7]宋益峰,兰林,吴江.我国典型浅水湖泊蓝藻水华治理技术研究进展[J].中国水运(下半月),2010,10(8):154-155
[8]Trochine C,Guerrieri M,Liboriussen L,et al.Filamentous green algae inhibit phytoplankton with enhanced effects when lakes get warmer[J].Freshwater Biology,2011,56(3):541-553
[9]Gao Y N,Dong J,Fu Q Q,et al.Allelopathic effects of submerged macrophytes on phytoplankton[J].Allelopathy Journal,2013,40(1):1-22
[10]Hilt S,Gross E M.Can allelopathically active submerged macrophytes stabilise clear-water states in shallow lakes?[J].Basic&Applied Ecology,2008,9(4):422-432
[11]Bonn M,Schmeck H.Allelopathic effect of the aquatic macrophyte,Stratiotes aloides,on natural phytoplankton[J].Freshwater Biology,2010,51(3):554-561
[12]Wu Y,Liu J,Yang L,et al.Allelopathic control of cyanobacterial blooms by periphyton biofilms[J].Environmental Microbiology,2011,13(3):604-615
[13]张维昊,周连凤,吴小刚,等.菖蒲对铜绿微囊藻的化感作用[J].中国环境科学,2006,26(3):355-358Zhang W H,Zhou L F,Wu X G,et al.Allelopathic effect of Acorus calamus on Microcystis aeruginosa[J].China Environmental Science,2006,26(3):355-358(in Chinese)
[14]李锋民,胡洪营.芦苇抑藻化感物质的分离及其抑制蛋白核小球藻效果研究[J].环境科学,2004,25(5):89-92Li F M,Hu H Y.Isolation and effects on green alga Chlorella pyrenoidosa of algal-inhibiting allelochemicals in the macrophyte,Phragmites communis Tris[J].Environmental Science,2004,25(5):89-92(in Chinese)
[15]李锋民,胡洪营,种云霄,等.芦苇化感物质EMA对铜绿微囊藻生理特性的影响[J].中国环境科学,2007,27(3):377-381Li F M,Hu H Y,Zhong Y X,et al.Influence of EMA isolated from Phragmites communis on physiological characters of Microcystis aeruginosa[J].China Environmental Science,2007,27(3):377-381(in Chinese)
[16]汪丽,王国祥,唐晓燕,等.荇菜(Nymphoides peltatum)对铜绿微囊藻(Microcystis aeruginosa)生长的抑制效应及其机制[J].生态与农村环境学报,2010,26(3):257-263Wang L,Wang G X,Tang X Y,et al.Inhibitory effect of Nymphoides peltatum on Microcystis aeruginosa and its mechanism[J].Journal of Ecology and Rural Environment,2010,26(3):257-263(in Chinese)
[17]董颖娜,冯彬,王冰璇,等.香蒲水浸提液对铜绿微囊藻的化感作用[J].生态学杂志,2018,37(2):498-505Dong Y N,Feng B,Wang B X,et al.Allelopathy of aqueous extract of cattail on Microcystis aeruginosa[J].Chinese Journal of Ecology,2018,37(2):498-505(in Chinese)
[18]吴富勤,刘天猛,王祖涛,等.滇池凤眼莲生长对水生植物的影响[J].安徽农业科学,2011,39(15):9167-9168Wu F Q,Liu T M,Wang Z T,et al.Effects of Eichhornia crassipes growth on aquatic plant in Dianchi Lake[J].Journal of Anhui Agricutural Science,2011,39(15):9167-9168(in Chinese)
[19]陈卫民,张清敏,戴树桂,等.苦草与铜绿微囊藻的相互化感作用[J].中国环境科学,2009,29(2):147-151Chen W M,Zhang Q M,Dai S G,et al.The mutual allelopathy of Vallisneria spiralis Linn.and Microcystis aeruginosa[J].China Environmental Science,2009,29(2):147-151(in Chinese)
[20]吴晓辉.常见眼子菜科沉水植物对浮游藻类的化感作用研究[D].北京:中国科学院研究生院,2005:1-154Wu X H.Studies on the allelopathy of several submerged Potamogetonaceae species on planktonic algae[D].Beijing:Graduate School of the Chinese Academy of Sciences,2005:1-154(in Chinese)
[21]肖溪,楼莉萍,李华,等.沉水植物化感作用控藻能力评述[J].应用生态学报,2009,20(3):705-712Xiao X,Lou L P,Li H,et al.Algal control ability of allelopathically active submerged macrophytes:A review[J].Chinese Journal of Applied Ecology,2009,20(3):705-712(in Chinese)
[22]白羽,黄莹莹,孔海南,等.加拿大一枝黄花化感抑藻效应的初步研究[J].生态环境学报,2014,21(7):1296-1303Bai Y,Huang Y Y,Kong H N,et al.Studies on the allelopathic effects of Solidago canadensis L on algae[J].Ecology and Environmental Sciences,2014,21(7):1296-1303(in Chinese)
[23]靳翠丽,周晓见,李楠,等.广玉兰提取物对铜绿微囊藻抑制作用的机制研究[J].环境污染与防治,2011,33(5):50-53Jin C L,Zhou X J,Li N,et al.The inhibitory mechanism of Magnolia grandiflora Linn.extracts on Microcystis aeruginosa[J].Environmental Pollution&Control,2011,33(5):50-53(in Chinese)
[24]王聪,张饮江,李岩,等.陆生植物化感作用抑制有害藻应用研究进展[J].环境科学与技术,2012,35(1):115-121Wang C,Zhang Y J,Li Y,et al.Research progress on allelopathic effects of terrestrial plants for inhibitting harmful algae[J].Environmental Science&Technology,2012,35(1):115-121(in Chinese)
[25]刘念江,吴莉宇,吴声敢.蟛蜞菊化感作用的初步研究[J].热带农业科学,2006,26(6):24-25Liu N J,Wu L Y,Wu S G.Preliminary study on allelopathic effects of Wedelia chinensis(Osbeck)Merrill[J].Chinese Journal of Tropical Agriculiure,2006,26(6):24-25(in Chinese)
[26]张玉虎,刘梅芳,凌铁军,等.三裂蟛蜞菊中的倍半萜内酯成分及其化感作用[J].热带亚热带植物学报,2004,12(6):533-537Zhang Y H,Liu M F,Ling T J,et al.Allelopathic sesquiterpene lactones from Wedelia chinensis[J].Journal of Tropical and Subtropical Botany,2004,12(6):533-537(in Chinese)
[27]曾任森,林象联,骆世明,等.蟛蜞菊的生化他感作用及生化他感作用物的分离鉴定[J].生态学报,1996,16(1):20-27Zeng R S,Lin X L,Luo S M,et al.Allelopathic potential of Wedelia chinensis and its allelochemicals[J].Acta Ecologica Sinica,1996,16(1):20-27(in Chinese)
[28]孔江红,谢钦铭,刘襄河,等.蟛蜞菊对铜绿微囊藻生长抑制作用研究[J].湖北农业科学,2011,50(4):712-716Kong J H,Xie Q M,Liu X H,et al.Study on the inhibition effect of Wedelia chinensis on the growth of Microcystis aeruginosa[J].Hubei Agricultural Sciences,2011,50(4):712-716(in Chinese)
[29]Gkelis S,Moustakagouni M,Sivonen K,et al.First report of the cyanobacterium Aphanizomenon ovalisporum Forti in two Greek lakes and cyanotoxin occurrence[J].Journal of Plankton Research,2005,27(12):167-174
[30]Messineo V,Melchiorre S,Corcia A D,et al.Seasonal succession of Cylindrospermopsis raciborskii and Aphanizomenon ovalisporum blooms with cylindrospermopsin occurrence in the volcanic Lake Albano,Central Italy[J].Environmental Toxicology,2010,25(1):18-27
[31]Quesada A,Moreno E,Carrasco D,et al.Toxicity of Aphanizomenon ovalisporum(Cyanobacteria)in a Spanish water reservoir[J].European Journal of Phycology,2006,41(1):39-45
[32]Pollingher U,Hadas O,Yacobi Y Z,et al.Aphanizomenon ovalisporum(Forti)in Lake Kinneret,Israel[J].Journal of Plankton Research,1998,20(7):1321-1339
[33]Cirés S,W9rmer L,Wiedner C,et al.Temperature-dependent dispersal strategies of Aphanizomenon ovalisporum(Nostocales,Cyanobacteria):Implications for the annual life cycle[J].Microbial Ecology,2013,65(1):12-21
[34]Mehnert G,Leunert F,Cirés S,et al.Competitiveness of invasive and native cyanobacteria from temperate freshwaters under various light and temperature conditions[J].Journal of Plankton Research,2010,32(7):1009-1021
[35]Pham M N,Tan H T W,Mitrovic S,et al.A Checklist of the Algae of Singapore[M].Singapore:Raffles Museum of Biodiversity Research,National University of Singapore,2011:1-100
[36]Medvedeva L A,Nikulina T V.Catalogue of Freshwater Algae of the Southern Part of the Russian Far East[M].Vladivostok:Dalnauka,2014:1-273
[37]Vaccarino M A,Johansen J R.The algal flora of Acadia National Park,Maine[J].Northeastern Naturalist,2015,18(4):457-474
[38]Zhang W,Jeppesen E,Wang M,et al.Allelopathic effect boosts Chrysosporum ovalisporum dominance in summer at the expense of Microcystis panniformis in a shallow coastal water body[J].Environmental Science and Pollution Research International,2017,24(5):1-10
[39]Juneau P,Harrison P J.Comparison by PAM fluorometry of photosynthetic activity of nine marine phytoplankton grown under identical conditions[J].Photochemistry&Photobiology,2005,81(3):649-653
[40]Padgett M P,Krogmann D W.Large scale preparation of pure phycobiliproteins[J].Photosynthesis Research,1987,11(3):225-235
[41]国家环境保护总局.水和废水监测分析方法.第4版[M].北京:中国环境科学出版社,2002:722-723
[42]Pesce S,Margoum C,Montuelle B.In situ relationships between spatio-temporal variations in diuron concentrations and phototrophic biofilm tolerance in a contaminated river[J].Water Research,2010,44(6):1941
[43]张庭廷,吴安平,何梅,等.酚酸类物质对水华藻类的化感作用及其机理[J].中国环境科学,2007,27(4):472-476Zhang T T,Wu A P,He M,et al.The allelopathy and its mechanism of phenolic acids on water-bloom algae[J].China Environmental Science,2007,27(4):472-476(in Chinese)
[44]王家春,陈应华,吴常文.加拿大一枝黄花有机溶剂提取液对东海原甲藻化感作用的研究[J].中国水运(月刊),2016,16(5):144-148
[45]Nakai S,Inoue Y,Hosomi M.Algal growth inhibition effects and inducement modes by plant-producing phenols[J].Water Research,2001,35(7):1855-1859
[46]李小路,潘慧云,徐洁,等.金鱼藻与铜绿微囊藻共生情况下的化感作用[J].环境科学学报,2008,28(11):2243-2249Li X L,Pan H Y,Xu J,et al.Allelopathic effects of Ceratophyllum demersum and Microcystis aeruginosa in co-cultivation[J].Acta Scientiae Circumstantiae,2008,28(11):2243-2249(in Chinese)
[47]吴程,常学秀,吴锋,等.高等水生植物对集胞藻(Synahocystis sp.)的化感作用研究[J].云南大学学报:自然科学版,2008,30(5):535-540Wu C,Chang X X,Wu F,et al.Studies on allelopathy of aquatic macrophytes on Synahocystis sp.[J].Journal of Yunnan University:Natural Sciences Edition,2008,30(5):535-540(in Chinese)
[48]Walsby A E,Hayes P K,Boje R,et al.The selective advantage of buoyancy provided by gas vesicles for planktonic cyanobacteria in the Baltic Sea[J].New Phytologist,1997,136(3):407-417
[49]Noyma N P,Silva T P,Chiarinigarcia H,et al.Potential effects of UV radiation on photosynthetic structures of the bloom-forming cyanobacterium Cylindrospermopsis raciborskii CYRF-01[J].Frontiers in Microbiology,2015,6(63):1202
[50]裴毅,杜宪,聂江力,等.没食子酸对铜绿微囊藻的化感作用[J].植物研究,2014,34(6):840-844Pei Y,Du X,Nie J L,et al.Allelopathy of gallic acid on Microcystis aeruginos[J].Bullein of Botanical Research,2014,34(6):840-844(in Chinese)
[51]Habig W H,Pabst M J,Jakoby W B.Glutathione S-transferases.The first enzymatic step in mercapturic acid formation[J].Journal of Biological Chemistry,1974,249(22):7130
[52]Felisa W,Daniel G,Oscar S,et al.The role and evolution of superoxide dismutases in algae[J].Journal of Phycology,2010,41(3):453-465
[53]李庆华,郭沛涌,田美燕,等.柳树叶浸提液对蛋白核小球藻的化感作用[J].生态学杂志,2009,28(5):884-888Li Q H,Guo P Y,Tian M Y,et al.Allelopathy of Salix babylonica leaf extracts on Chlorella pyrenoidos[J].Chinese Journal of Ecology,2009,28(5):884-888(in Chinese)
[2]王扬才,陆开宏.蓝藻水华的危害及治理动态[J].水产学杂志,2004,17(1):90-94Wang Y C,Lu K H.Harmful effects of cyanobacteria and its control development[J].Chinese Journal of Fisheries,2004,17(1):90-94(in Chinese)
[3]Paul V J.Global warming and cyanobacterial harmful algal blooms[J].Advances in Experimental Medicine&Biology,2008,619:239-257
[4]Kosten S,Huszar V L M,Bécares E,et al.Warmer climates boost cyanobacterial dominance in shallow lakes[J].Global Change Biology,2012,18(1):118-126
[5]孔繁翔.湖泊富营养化治理与蓝藻水华控制[J].江苏科技信息,2007(9):7-11
[6]陈识文,毛涛,袁科平,等.水华治理方法研究进展[J].长江大学学报:自科版,2014,11(35):69-73
[7]宋益峰,兰林,吴江.我国典型浅水湖泊蓝藻水华治理技术研究进展[J].中国水运(下半月),2010,10(8):154-155
[8]Trochine C,Guerrieri M,Liboriussen L,et al.Filamentous green algae inhibit phytoplankton with enhanced effects when lakes get warmer[J].Freshwater Biology,2011,56(3):541-553
[9]Gao Y N,Dong J,Fu Q Q,et al.Allelopathic effects of submerged macrophytes on phytoplankton[J].Allelopathy Journal,2013,40(1):1-22
[10]Hilt S,Gross E M.Can allelopathically active submerged macrophytes stabilise clear-water states in shallow lakes?[J].Basic&Applied Ecology,2008,9(4):422-432
[11]Bonn M,Schmeck H.Allelopathic effect of the aquatic macrophyte,Stratiotes aloides,on natural phytoplankton[J].Freshwater Biology,2010,51(3):554-561
[12]Wu Y,Liu J,Yang L,et al.Allelopathic control of cyanobacterial blooms by periphyton biofilms[J].Environmental Microbiology,2011,13(3):604-615
[13]张维昊,周连凤,吴小刚,等.菖蒲对铜绿微囊藻的化感作用[J].中国环境科学,2006,26(3):355-358Zhang W H,Zhou L F,Wu X G,et al.Allelopathic effect of Acorus calamus on Microcystis aeruginosa[J].China Environmental Science,2006,26(3):355-358(in Chinese)
[14]李锋民,胡洪营.芦苇抑藻化感物质的分离及其抑制蛋白核小球藻效果研究[J].环境科学,2004,25(5):89-92Li F M,Hu H Y.Isolation and effects on green alga Chlorella pyrenoidosa of algal-inhibiting allelochemicals in the macrophyte,Phragmites communis Tris[J].Environmental Science,2004,25(5):89-92(in Chinese)
[15]李锋民,胡洪营,种云霄,等.芦苇化感物质EMA对铜绿微囊藻生理特性的影响[J].中国环境科学,2007,27(3):377-381Li F M,Hu H Y,Zhong Y X,et al.Influence of EMA isolated from Phragmites communis on physiological characters of Microcystis aeruginosa[J].China Environmental Science,2007,27(3):377-381(in Chinese)
[16]汪丽,王国祥,唐晓燕,等.荇菜(Nymphoides peltatum)对铜绿微囊藻(Microcystis aeruginosa)生长的抑制效应及其机制[J].生态与农村环境学报,2010,26(3):257-263Wang L,Wang G X,Tang X Y,et al.Inhibitory effect of Nymphoides peltatum on Microcystis aeruginosa and its mechanism[J].Journal of Ecology and Rural Environment,2010,26(3):257-263(in Chinese)
[17]董颖娜,冯彬,王冰璇,等.香蒲水浸提液对铜绿微囊藻的化感作用[J].生态学杂志,2018,37(2):498-505Dong Y N,Feng B,Wang B X,et al.Allelopathy of aqueous extract of cattail on Microcystis aeruginosa[J].Chinese Journal of Ecology,2018,37(2):498-505(in Chinese)
[18]吴富勤,刘天猛,王祖涛,等.滇池凤眼莲生长对水生植物的影响[J].安徽农业科学,2011,39(15):9167-9168Wu F Q,Liu T M,Wang Z T,et al.Effects of Eichhornia crassipes growth on aquatic plant in Dianchi Lake[J].Journal of Anhui Agricutural Science,2011,39(15):9167-9168(in Chinese)
[19]陈卫民,张清敏,戴树桂,等.苦草与铜绿微囊藻的相互化感作用[J].中国环境科学,2009,29(2):147-151Chen W M,Zhang Q M,Dai S G,et al.The mutual allelopathy of Vallisneria spiralis Linn.and Microcystis aeruginosa[J].China Environmental Science,2009,29(2):147-151(in Chinese)
[20]吴晓辉.常见眼子菜科沉水植物对浮游藻类的化感作用研究[D].北京:中国科学院研究生院,2005:1-154Wu X H.Studies on the allelopathy of several submerged Potamogetonaceae species on planktonic algae[D].Beijing:Graduate School of the Chinese Academy of Sciences,2005:1-154(in Chinese)
[21]肖溪,楼莉萍,李华,等.沉水植物化感作用控藻能力评述[J].应用生态学报,2009,20(3):705-712Xiao X,Lou L P,Li H,et al.Algal control ability of allelopathically active submerged macrophytes:A review[J].Chinese Journal of Applied Ecology,2009,20(3):705-712(in Chinese)
[22]白羽,黄莹莹,孔海南,等.加拿大一枝黄花化感抑藻效应的初步研究[J].生态环境学报,2014,21(7):1296-1303Bai Y,Huang Y Y,Kong H N,et al.Studies on the allelopathic effects of Solidago canadensis L on algae[J].Ecology and Environmental Sciences,2014,21(7):1296-1303(in Chinese)
[23]靳翠丽,周晓见,李楠,等.广玉兰提取物对铜绿微囊藻抑制作用的机制研究[J].环境污染与防治,2011,33(5):50-53Jin C L,Zhou X J,Li N,et al.The inhibitory mechanism of Magnolia grandiflora Linn.extracts on Microcystis aeruginosa[J].Environmental Pollution&Control,2011,33(5):50-53(in Chinese)
[24]王聪,张饮江,李岩,等.陆生植物化感作用抑制有害藻应用研究进展[J].环境科学与技术,2012,35(1):115-121Wang C,Zhang Y J,Li Y,et al.Research progress on allelopathic effects of terrestrial plants for inhibitting harmful algae[J].Environmental Science&Technology,2012,35(1):115-121(in Chinese)
[25]刘念江,吴莉宇,吴声敢.蟛蜞菊化感作用的初步研究[J].热带农业科学,2006,26(6):24-25Liu N J,Wu L Y,Wu S G.Preliminary study on allelopathic effects of Wedelia chinensis(Osbeck)Merrill[J].Chinese Journal of Tropical Agriculiure,2006,26(6):24-25(in Chinese)
[26]张玉虎,刘梅芳,凌铁军,等.三裂蟛蜞菊中的倍半萜内酯成分及其化感作用[J].热带亚热带植物学报,2004,12(6):533-537Zhang Y H,Liu M F,Ling T J,et al.Allelopathic sesquiterpene lactones from Wedelia chinensis[J].Journal of Tropical and Subtropical Botany,2004,12(6):533-537(in Chinese)
[27]曾任森,林象联,骆世明,等.蟛蜞菊的生化他感作用及生化他感作用物的分离鉴定[J].生态学报,1996,16(1):20-27Zeng R S,Lin X L,Luo S M,et al.Allelopathic potential of Wedelia chinensis and its allelochemicals[J].Acta Ecologica Sinica,1996,16(1):20-27(in Chinese)
[28]孔江红,谢钦铭,刘襄河,等.蟛蜞菊对铜绿微囊藻生长抑制作用研究[J].湖北农业科学,2011,50(4):712-716Kong J H,Xie Q M,Liu X H,et al.Study on the inhibition effect of Wedelia chinensis on the growth of Microcystis aeruginosa[J].Hubei Agricultural Sciences,2011,50(4):712-716(in Chinese)
[29]Gkelis S,Moustakagouni M,Sivonen K,et al.First report of the cyanobacterium Aphanizomenon ovalisporum Forti in two Greek lakes and cyanotoxin occurrence[J].Journal of Plankton Research,2005,27(12):167-174
[30]Messineo V,Melchiorre S,Corcia A D,et al.Seasonal succession of Cylindrospermopsis raciborskii and Aphanizomenon ovalisporum blooms with cylindrospermopsin occurrence in the volcanic Lake Albano,Central Italy[J].Environmental Toxicology,2010,25(1):18-27
[31]Quesada A,Moreno E,Carrasco D,et al.Toxicity of Aphanizomenon ovalisporum(Cyanobacteria)in a Spanish water reservoir[J].European Journal of Phycology,2006,41(1):39-45
[32]Pollingher U,Hadas O,Yacobi Y Z,et al.Aphanizomenon ovalisporum(Forti)in Lake Kinneret,Israel[J].Journal of Plankton Research,1998,20(7):1321-1339
[33]Cirés S,W9rmer L,Wiedner C,et al.Temperature-dependent dispersal strategies of Aphanizomenon ovalisporum(Nostocales,Cyanobacteria):Implications for the annual life cycle[J].Microbial Ecology,2013,65(1):12-21
[34]Mehnert G,Leunert F,Cirés S,et al.Competitiveness of invasive and native cyanobacteria from temperate freshwaters under various light and temperature conditions[J].Journal of Plankton Research,2010,32(7):1009-1021
[35]Pham M N,Tan H T W,Mitrovic S,et al.A Checklist of the Algae of Singapore[M].Singapore:Raffles Museum of Biodiversity Research,National University of Singapore,2011:1-100
[36]Medvedeva L A,Nikulina T V.Catalogue of Freshwater Algae of the Southern Part of the Russian Far East[M].Vladivostok:Dalnauka,2014:1-273
[37]Vaccarino M A,Johansen J R.The algal flora of Acadia National Park,Maine[J].Northeastern Naturalist,2015,18(4):457-474
[38]Zhang W,Jeppesen E,Wang M,et al.Allelopathic effect boosts Chrysosporum ovalisporum dominance in summer at the expense of Microcystis panniformis in a shallow coastal water body[J].Environmental Science and Pollution Research International,2017,24(5):1-10
[39]Juneau P,Harrison P J.Comparison by PAM fluorometry of photosynthetic activity of nine marine phytoplankton grown under identical conditions[J].Photochemistry&Photobiology,2005,81(3):649-653
[40]Padgett M P,Krogmann D W.Large scale preparation of pure phycobiliproteins[J].Photosynthesis Research,1987,11(3):225-235
[41]国家环境保护总局.水和废水监测分析方法.第4版[M].北京:中国环境科学出版社,2002:722-723
[42]Pesce S,Margoum C,Montuelle B.In situ relationships between spatio-temporal variations in diuron concentrations and phototrophic biofilm tolerance in a contaminated river[J].Water Research,2010,44(6):1941
[43]张庭廷,吴安平,何梅,等.酚酸类物质对水华藻类的化感作用及其机理[J].中国环境科学,2007,27(4):472-476Zhang T T,Wu A P,He M,et al.The allelopathy and its mechanism of phenolic acids on water-bloom algae[J].China Environmental Science,2007,27(4):472-476(in Chinese)
[44]王家春,陈应华,吴常文.加拿大一枝黄花有机溶剂提取液对东海原甲藻化感作用的研究[J].中国水运(月刊),2016,16(5):144-148
[45]Nakai S,Inoue Y,Hosomi M.Algal growth inhibition effects and inducement modes by plant-producing phenols[J].Water Research,2001,35(7):1855-1859
[46]李小路,潘慧云,徐洁,等.金鱼藻与铜绿微囊藻共生情况下的化感作用[J].环境科学学报,2008,28(11):2243-2249Li X L,Pan H Y,Xu J,et al.Allelopathic effects of Ceratophyllum demersum and Microcystis aeruginosa in co-cultivation[J].Acta Scientiae Circumstantiae,2008,28(11):2243-2249(in Chinese)
[47]吴程,常学秀,吴锋,等.高等水生植物对集胞藻(Synahocystis sp.)的化感作用研究[J].云南大学学报:自然科学版,2008,30(5):535-540Wu C,Chang X X,Wu F,et al.Studies on allelopathy of aquatic macrophytes on Synahocystis sp.[J].Journal of Yunnan University:Natural Sciences Edition,2008,30(5):535-540(in Chinese)
[48]Walsby A E,Hayes P K,Boje R,et al.The selective advantage of buoyancy provided by gas vesicles for planktonic cyanobacteria in the Baltic Sea[J].New Phytologist,1997,136(3):407-417
[49]Noyma N P,Silva T P,Chiarinigarcia H,et al.Potential effects of UV radiation on photosynthetic structures of the bloom-forming cyanobacterium Cylindrospermopsis raciborskii CYRF-01[J].Frontiers in Microbiology,2015,6(63):1202
[50]裴毅,杜宪,聂江力,等.没食子酸对铜绿微囊藻的化感作用[J].植物研究,2014,34(6):840-844Pei Y,Du X,Nie J L,et al.Allelopathy of gallic acid on Microcystis aeruginos[J].Bullein of Botanical Research,2014,34(6):840-844(in Chinese)
[51]Habig W H,Pabst M J,Jakoby W B.Glutathione S-transferases.The first enzymatic step in mercapturic acid formation[J].Journal of Biological Chemistry,1974,249(22):7130
[52]Felisa W,Daniel G,Oscar S,et al.The role and evolution of superoxide dismutases in algae[J].Journal of Phycology,2010,41(3):453-465
[53]李庆华,郭沛涌,田美燕,等.柳树叶浸提液对蛋白核小球藻的化感作用[J].生态学杂志,2009,28(5):884-888Li Q H,Guo P Y,Tian M Y,et al.Allelopathy of Salix babylonica leaf extracts on Chlorella pyrenoidos[J].Chinese Journal of Ecology,2009,28(5):884-888(in Chinese)
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