芝麻素对氟胁迫下斑马鱼头部抗氧化功能和免疫相关酶活性的影响
|
摘要
为了探讨芝麻素对氟胁迫下斑马鱼头部抗氧化功能和非特异性免疫的影响,采用半静态法,将驯养后的斑马鱼,随机分为对照组(CK)、1 g/kg芝麻素组(S1)、2 g/kg芝麻素组(S2),80 mg/L氟胁迫组(F)、1 g/kg芝麻素组+80 mg/L氟胁迫组(FS1)、2 g/kg芝麻素组+80 mg/L氟胁迫组(FS2),分别在试验进行45、90 d时取斑马鱼头部,并对头部组织中的活性氧(ROS)、超氧化物歧化酶(SOD)、丙二醛(MDA)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)进行测定。结果表明,与F组比较,饲粮中添加芝麻素的FS1和FS2组斑马鱼头部ROS、MDA含量明显下降(P<0.05),而SOD活性明显上升(P<0.05),免疫相关酶ACP和AKP活性明显上升(P<0.05)。由此可见,饵料中添加一定剂量的芝麻素后,可以在一定程度上缓解氟胁迫对斑马鱼头部造成的氧化应激和免疫毒性作用。
In order to investigate the effect of sesamin on the antioxidant function and non-specific immunity of head in zebrafish exposed to fluoride, zebrafishes were randomly divided into control group(CK), 1 g/kg sesamin group(S1), 2 g/kg sesamin group(S2), 80 mg/L fluoride group(F),1 g/kg sesamin + 80 mg/L fluoride group(FS1), and 2 g/kg sesamin + 80 mg/L fluoride group(FS2), respectively. After exposure for 45 and 90 days, the reactive oxygen species(ROS), superoxide dismutase(SOD), malondialdehyde(MDA),alkaline phosphatase(AKP) and acid phosphatase(ACP) in the head of zebrafish were measured. The results showed that ROS and MDA in FS1 and FS2 groups were significantly decreased compared with the F group(P <0.05), while SOD activity, the activities of ACP and AKP in FS1 and FS2 groups was remarkably increased compared with the F group(P<0.05). All above results demonstrated that the addition of a certain dose of sesamin could alleviate the oxidative stress and immunotoxicity of head in zebrafish caused by fluoride to some extent.
In order to investigate the effect of sesamin on the antioxidant function and non-specific immunity of head in zebrafish exposed to fluoride, zebrafishes were randomly divided into control group(CK), 1 g/kg sesamin group(S1), 2 g/kg sesamin group(S2), 80 mg/L fluoride group(F),1 g/kg sesamin + 80 mg/L fluoride group(FS1), and 2 g/kg sesamin + 80 mg/L fluoride group(FS2), respectively. After exposure for 45 and 90 days, the reactive oxygen species(ROS), superoxide dismutase(SOD), malondialdehyde(MDA),alkaline phosphatase(AKP) and acid phosphatase(ACP) in the head of zebrafish were measured. The results showed that ROS and MDA in FS1 and FS2 groups were significantly decreased compared with the F group(P <0.05), while SOD activity, the activities of ACP and AKP in FS1 and FS2 groups was remarkably increased compared with the F group(P<0.05). All above results demonstrated that the addition of a certain dose of sesamin could alleviate the oxidative stress and immunotoxicity of head in zebrafish caused by fluoride to some extent.
引文
[1]陈剑杰,曹谨玲,李潇,等.氟对中华圆田螺肝胰脏抗氧化酶活性和MDA含量的影响[J].生态毒理学报,2018,13(1):268-273.
[2]郭文静,王天玉,薛文娟,等.氟对斑马鱼甲状腺内分泌功能的干扰效应[J].环境科学学报,2019,39(4):1340-1347.
[3] Cao J L, Chen J J, Wang J D, et al. Effects of sodium fluoride on MAPKs signaling pathway in the gills of a freshwater teleost, Cyprinus carpio[J].Aquatic Toxicol., 2014, 158:164-172.
[4] Dhar V, Bhatnagar M. Physiology and toxicity of fluoride[J]. Indian J.Dent. Res., 2009, 20:350-355.
[5] Zhang J, Zhu Y, Shi Y, et al. Fluoride-Induced Autophagy via the Regulation of Phosphorylation of Mammalian Targets of Rapamycin in Mice Leydig Cells[J]. Journal of Agricultural and Food Chemistry, 2017,65(40):8966-8976.
[6]柳照应.氟化物对家蚕细胞色素P450影响的研究[D].重庆:西南大学,2009.
[7]毕翻,魏娜,万珑.氟对大鼠海马神经细胞磷酸化-NMDAR蛋白表达的影响[J].中华地方病学杂志,2019,38(1):4-7.
[8]刘洋,李艳菊,王宁,等.p53、p21在氟中毒雌鼠卵巢早衰中的表达变化[J].重庆医学,2018, 47(13):1712-1715.
[9] Cao J L, Chen J J, Wang J D, et al. Effects of fluoride on liver apoptosis and Bcl-2, Bax protein expression in freshwater teleost, Cyprinus carpio[J]. Chemosphere, 2013, 91:1203-1212.
[10]李学会,夏曙华,刘咏梅,等.低蛋白摄入对燃煤型氟中毒雌性大鼠卵巢胰岛素样生长因子-1表达的影响[J].环境与健康杂志,2018,35(5):402-404.
[11]BhatnagarC,BhatnagarM,RegarBC.Fluoride-induced histopathological changes in gill, kidney, and intestine of fresh water teleost, Labeo rohita[J]. Fluoride, 2007, 40(1):55-61.
[12] Cao J L, Chen J J, Wang J D, et al. Tissue distributions of fluoride and its toxicity in the gills of a freshwater teleost, Cyprinus carpio[J]. Aquatic Toxicol., 2013, 130:68-76.
[13]李俊骏,赵巧湜,李悦,等.胚胎期氟暴露对斑马鱼骨骼发育的影响[J].中华地方病学杂志,2018,37(1):24-29.
[14] Singh R, Khatri P, Srivastava N, et al. Fluoride exposure abates proinflammatory response and induces in vivo apoptosis rendering zebrafish(Danio rerio)susceptible to bacterial infections[J]. Fish&Shellfish Immunology, 2017, 63(4):314-321.
[15] Sasimol U, Chayanut K, Peraphan P, et al. Sesamin suppresses LPSinduced microglial activation via regulation of TLR4 expression[J].Journal of Functional Foods, 2018, 49:32-43.
[16] Cao J L, Chen J J, Xie L T, et al. Protective properties of sesamin against fluoride-induced oxidative stress and apoptosis in kidney of carp(Cyprinus carpio)via JNK signaling pathway[J]. Aquatic Toxicology,2015, 167:180-190.
[17]李萍,刘华钢.氯化两面针碱和芝麻素对斑马鱼胚胎的毒性效应研究[J].中药药理与临床,2018,34(1):101-104.
[18]汪五三,宋建国.芝麻素保肝作用试验研究[J].中药药理与临床,2006,22(3):27-32.
[19]田俊芝,张荣,张宏学,等.芝麻素对氯化镉心肌损伤的保护作用[J].中华劳动卫生职业病杂志,2014,32(5):357-360.
[20]杨丽珍,张国刚.氧化应激与动脉粥样硬化[J].医学与哲学,2013(5):54-56.
[21]陈修报,刘洪波,苏彦平,等.镉对“标准化”背角无齿蚌的急性毒性及脂质过氧化和DNA损伤的影响[J].农业环境科学学报,2017,36(10):1960-1967.
[22]陈家长,孟顺龙,胡庚东,等.低浓度阿特拉津对鲫鱼过氧化氢酶活性的影响[J].农业环境科学学报,2008,27(3):1151-1156.
[23]陈剑杰,曹谨玲,宋杰,等.氟对鲤鱼肾抗氧化酶和Na+-K+-ATPase水平的影响[J].核农学报,2016,30(5):1030-1035.
[24]吴红松.长期铈喂养对鲤鱼肝胰脏、肾脏抗氧化酶活性的影响[J].核农学报,2017,31(1):201-207.
[25]余日安,夏涛,王爱国,等.氟中毒对大鼠肾脏脂质过氧化、细胞坏死、凋亡及增殖周期的影响[J].环境与健康杂志,2001,18(6):336-338.
[26]王飞清,李艳菊,王宁,等.氧化应激-炎性因子对氟中毒卵巢早衰的影响[J].现代预防医学,2017,44(12):2296-2299.
[27]张璟,章子贵,申秀英,等.慢性氟中毒与脑损伤关系研究进展[J].中国公共卫生,2010,26(2):251-253.
[28]刚亚栋.氟致大鼠肾脏毒性及其机理研究[D].兰州:兰州大学,2010.
[29]彭金砖,汪学德,管军军.芝麻木酚素抗氧化性的研究进展[J].粮油食品科技,2013,21(6):38-40.
[30]张鑫,李方方,朱宇旌,等.芝麻木酚素的抗氧化性及其机制研究[J].猪业科学,2015,6:84-85.
[31]李先伟,杨解人,朱萧俊.芝麻素对肾性高血压大鼠血流动力学、心肌一氧化氮和内皮素-1的影响[J].中国试验方剂学杂志,2007(4):33-36.
[32]郑书国,任尤楠,赵梦秋,等.芝麻素含药血清对血管紧张素Ⅱ诱导大鼠心肌细胞凋亡的影响[J].中药材,2015,38(5):1013-1017.
[33]饶海军,唐向阳,周远辉,等.芝麻素通过抑制氧化应激以及抗凋亡作用保护大鼠脊髓损伤[J].神经损伤与功能重建,2018,13(5):244-246.
[34]李赫,宋文华,于翔,等.几种免疫增强剂对草鱼SOD、CAT及AKP活性的影响[J].水产学杂志,2010,23(4):6-9.
[35]罗莎,王秋举,鞠雪,等.氧化鱼油对草鱼幼鱼磷酸酶和转氨酶活性的影响[J].吉林农业大学学报,2016,38(3):357-363.
[36]高举,赵欣平,詹付凤,等.铅对鲫鱼碱性磷酸酶和酸性磷酸酶活性的影响[J].四川动物,2008,27(2):201-204.
[37]陈志伟,贾秀英.氟对家蚕血液及中肠磷酸酶活性的影响[J].农业环境科学学报,2002,21(2):130-133.
[38]刘彦群,延光海,秦向征,等.芝麻素通过调节p38和NF-κB激活抑制肥大细胞炎症细胞因子释放[J].免疫学杂志,2014,30(12):1073-1076.
[39]赵洪伟,崔银锋,姜京植,等.芝麻素通过PKCα/NF-κB信号途径抑制肥大细胞活化[J].中国免疫学杂志,2018,34(2):167-171.
[2]郭文静,王天玉,薛文娟,等.氟对斑马鱼甲状腺内分泌功能的干扰效应[J].环境科学学报,2019,39(4):1340-1347.
[3] Cao J L, Chen J J, Wang J D, et al. Effects of sodium fluoride on MAPKs signaling pathway in the gills of a freshwater teleost, Cyprinus carpio[J].Aquatic Toxicol., 2014, 158:164-172.
[4] Dhar V, Bhatnagar M. Physiology and toxicity of fluoride[J]. Indian J.Dent. Res., 2009, 20:350-355.
[5] Zhang J, Zhu Y, Shi Y, et al. Fluoride-Induced Autophagy via the Regulation of Phosphorylation of Mammalian Targets of Rapamycin in Mice Leydig Cells[J]. Journal of Agricultural and Food Chemistry, 2017,65(40):8966-8976.
[6]柳照应.氟化物对家蚕细胞色素P450影响的研究[D].重庆:西南大学,2009.
[7]毕翻,魏娜,万珑.氟对大鼠海马神经细胞磷酸化-NMDAR蛋白表达的影响[J].中华地方病学杂志,2019,38(1):4-7.
[8]刘洋,李艳菊,王宁,等.p53、p21在氟中毒雌鼠卵巢早衰中的表达变化[J].重庆医学,2018, 47(13):1712-1715.
[9] Cao J L, Chen J J, Wang J D, et al. Effects of fluoride on liver apoptosis and Bcl-2, Bax protein expression in freshwater teleost, Cyprinus carpio[J]. Chemosphere, 2013, 91:1203-1212.
[10]李学会,夏曙华,刘咏梅,等.低蛋白摄入对燃煤型氟中毒雌性大鼠卵巢胰岛素样生长因子-1表达的影响[J].环境与健康杂志,2018,35(5):402-404.
[11]BhatnagarC,BhatnagarM,RegarBC.Fluoride-induced histopathological changes in gill, kidney, and intestine of fresh water teleost, Labeo rohita[J]. Fluoride, 2007, 40(1):55-61.
[12] Cao J L, Chen J J, Wang J D, et al. Tissue distributions of fluoride and its toxicity in the gills of a freshwater teleost, Cyprinus carpio[J]. Aquatic Toxicol., 2013, 130:68-76.
[13]李俊骏,赵巧湜,李悦,等.胚胎期氟暴露对斑马鱼骨骼发育的影响[J].中华地方病学杂志,2018,37(1):24-29.
[14] Singh R, Khatri P, Srivastava N, et al. Fluoride exposure abates proinflammatory response and induces in vivo apoptosis rendering zebrafish(Danio rerio)susceptible to bacterial infections[J]. Fish&Shellfish Immunology, 2017, 63(4):314-321.
[15] Sasimol U, Chayanut K, Peraphan P, et al. Sesamin suppresses LPSinduced microglial activation via regulation of TLR4 expression[J].Journal of Functional Foods, 2018, 49:32-43.
[16] Cao J L, Chen J J, Xie L T, et al. Protective properties of sesamin against fluoride-induced oxidative stress and apoptosis in kidney of carp(Cyprinus carpio)via JNK signaling pathway[J]. Aquatic Toxicology,2015, 167:180-190.
[17]李萍,刘华钢.氯化两面针碱和芝麻素对斑马鱼胚胎的毒性效应研究[J].中药药理与临床,2018,34(1):101-104.
[18]汪五三,宋建国.芝麻素保肝作用试验研究[J].中药药理与临床,2006,22(3):27-32.
[19]田俊芝,张荣,张宏学,等.芝麻素对氯化镉心肌损伤的保护作用[J].中华劳动卫生职业病杂志,2014,32(5):357-360.
[20]杨丽珍,张国刚.氧化应激与动脉粥样硬化[J].医学与哲学,2013(5):54-56.
[21]陈修报,刘洪波,苏彦平,等.镉对“标准化”背角无齿蚌的急性毒性及脂质过氧化和DNA损伤的影响[J].农业环境科学学报,2017,36(10):1960-1967.
[22]陈家长,孟顺龙,胡庚东,等.低浓度阿特拉津对鲫鱼过氧化氢酶活性的影响[J].农业环境科学学报,2008,27(3):1151-1156.
[23]陈剑杰,曹谨玲,宋杰,等.氟对鲤鱼肾抗氧化酶和Na+-K+-ATPase水平的影响[J].核农学报,2016,30(5):1030-1035.
[24]吴红松.长期铈喂养对鲤鱼肝胰脏、肾脏抗氧化酶活性的影响[J].核农学报,2017,31(1):201-207.
[25]余日安,夏涛,王爱国,等.氟中毒对大鼠肾脏脂质过氧化、细胞坏死、凋亡及增殖周期的影响[J].环境与健康杂志,2001,18(6):336-338.
[26]王飞清,李艳菊,王宁,等.氧化应激-炎性因子对氟中毒卵巢早衰的影响[J].现代预防医学,2017,44(12):2296-2299.
[27]张璟,章子贵,申秀英,等.慢性氟中毒与脑损伤关系研究进展[J].中国公共卫生,2010,26(2):251-253.
[28]刚亚栋.氟致大鼠肾脏毒性及其机理研究[D].兰州:兰州大学,2010.
[29]彭金砖,汪学德,管军军.芝麻木酚素抗氧化性的研究进展[J].粮油食品科技,2013,21(6):38-40.
[30]张鑫,李方方,朱宇旌,等.芝麻木酚素的抗氧化性及其机制研究[J].猪业科学,2015,6:84-85.
[31]李先伟,杨解人,朱萧俊.芝麻素对肾性高血压大鼠血流动力学、心肌一氧化氮和内皮素-1的影响[J].中国试验方剂学杂志,2007(4):33-36.
[32]郑书国,任尤楠,赵梦秋,等.芝麻素含药血清对血管紧张素Ⅱ诱导大鼠心肌细胞凋亡的影响[J].中药材,2015,38(5):1013-1017.
[33]饶海军,唐向阳,周远辉,等.芝麻素通过抑制氧化应激以及抗凋亡作用保护大鼠脊髓损伤[J].神经损伤与功能重建,2018,13(5):244-246.
[34]李赫,宋文华,于翔,等.几种免疫增强剂对草鱼SOD、CAT及AKP活性的影响[J].水产学杂志,2010,23(4):6-9.
[35]罗莎,王秋举,鞠雪,等.氧化鱼油对草鱼幼鱼磷酸酶和转氨酶活性的影响[J].吉林农业大学学报,2016,38(3):357-363.
[36]高举,赵欣平,詹付凤,等.铅对鲫鱼碱性磷酸酶和酸性磷酸酶活性的影响[J].四川动物,2008,27(2):201-204.
[37]陈志伟,贾秀英.氟对家蚕血液及中肠磷酸酶活性的影响[J].农业环境科学学报,2002,21(2):130-133.
[38]刘彦群,延光海,秦向征,等.芝麻素通过调节p38和NF-κB激活抑制肥大细胞炎症细胞因子释放[J].免疫学杂志,2014,30(12):1073-1076.
[39]赵洪伟,崔银锋,姜京植,等.芝麻素通过PKCα/NF-κB信号途径抑制肥大细胞活化[J].中国免疫学杂志,2018,34(2):167-171.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |